| 质粒名称: | pAAVLINK-EF1a-HA-5'-HECW2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001500 |
| 产品用途: | 5' AAVLINK plasmid for HECW2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | HECW2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-HECW2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-HECW2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-HECW2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-HECW2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-HECW2质粒载体简介
pAAVLINK-EF1a-HA-5'-HECW2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-HECW2 6610 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6610)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6610
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 169..711
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 198..409
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 422..690
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 516..521
/label=SDS
CDS 727..753
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 760..3750
/codon_start=1
/label=HECW2
/translation="ASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRA
NSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANF
WDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAV
MMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPT
CAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLT
IPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGV
NSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSFRTSSTLEIDTEELT
STSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNA
MLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEA
STHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPEPQPSADQGSAELCGSQEVDQPT
SGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGES
DLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAE
GSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQEELGEVWQRRGSLEGAAAAAESPP
QEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRSLPSVRKDVSRYQRVDEALPPNW
EARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTN
ERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRITLLLQSPPVKFLISPEFF
TVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPWG
WEMKHDHQ"
misc_feature 1007..1012
/label=SDS
misc_feature 2204..2209
/label=SDS
misc_feature 2207..2212
/label=SDS
misc_feature 2520..2525
/label=SDS
misc_feature 2786..2791
/label=SDS
misc_feature 2789..2794
/label=SDS
misc_feature 2945..2950
/label=SDS
misc_feature 3074..3079
/label=SDS
misc_feature 3077..3082
/label=SDS
intron 3751..3791
/label=Splicing Donor
misc_feature 3795..3828
/label=lox2272-JT1571
repeat_region 3873..4013
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3873..4002
/label=AAV2 ITR
rep_origin 4088..4543
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4825..4929
/gene="bla"
/label=AmpR promoter
CDS 4930..5790
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5961..6549
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
721 accatgtacc cttatgacgt acctgactat gctggatctg ctagttcagc ccgggagcac
781 ctgctttttg tgaggcgtcg aaatccccag atgcggtaca cattgagccc agagaacctc
841 cagagccttg ccgcccagag ctccatgcca gagaacatga ccctgcagcg ggccaacagc
901 gacaccgacc tggtgacttc tgagagccgc tccagcttaa ctgccagcat gtacgagtac
961 acgctggggc aagcccagaa cctcattatc ttctgggaca ttaaagagga ggtggacccc
1021 agtgattgga ttggacttta tcatatagat gagaattctc cagccaactt ctgggattct
1081 aagaacaggg gtgtgactgg aacacaaaaa gggcaaattg tatggagaat tgagcctggg
1141 ccctatttca tggaaccgga gataaaaatc tgttttaaat attaccacgg cattagtgga
1201 gccctgcgag ccacgacccc ctgcatcacc gtgaagaacc cagctgtgat gatgggggca
1261 gaaggcatgg agggaggtgc ttcaggaaac ctgcattctc gaaaacttgt tagctttaca
1321 ttgtcagatc ttagggcagt tgggctaaag aaagggatgt tcttcaatcc tgacccttat
1381 cttaagatgt caattcagcc aggaaagaag agcagtttcc ccacctgtgc ccaccacggg
1441 caggagagac ggtctactat catcagtaac accaccaatc caatttggca ccgagagaaa
1501 tattcctttt ttgcacttct tactgatgtc ttagaaattg aaattaaaga caaatttgcc
1561 aagagccgtc ccatcatcaa gcgttttctg gggaaactaa ccattccagt ccagaggctg
1621 ctggagcgac aagccatcgg tgatcaaatg ctcagctaca accttggcag aaggctccca
1681 gctgaccacg tgagtgggta cctccagttt aaagtggagg ttacgtcttc tgttcatgaa
1741 gatgcctctc cagaagctgt tggcacaata cttggagtca attctgtgaa tggagactta
1801 ggtagccctt ccgatgacga ggacatgcca gggagccatc acgacagcca ggtgtgctct
1861 aatgggccag tttctgagga cagtgctgcc gatggaaccc ccaagcattc attcaggact
1921 agctccactc tggaaataga cacagaggaa ttaacctcta cgtcttcaag gacctcacct
1981 cccagaggac gtcaggattc actcaatgat tacttagatg ctatcgaaca caatggccac
2041 tccaggccag ggacagcgac ctgctctgag aggtccatgg gtgcctctcc gaaactgagg
2101 agtagttttc ccactgacac aagactcaat gccatgcttc atattgattc agatgaagaa
2161 gatcacgagt tccagcaaga cctgggttac ccatcttcct tggaggagga gggaggcctg
2221 atcatgttta gcagggcatc cagagctgat gatggaagcc tgacatctca gacaaagctg
2281 gaggacaacc ctgttgagaa tgaggaagcc tccacacacg aagctgcttc ctttgaggat
2341 aagccagaaa atcttccaga gcttgcagag agctccttac ctgcaggccc agccccagag
2401 gaaggtgaag gcggcccaga gcctcaaccc agtgctgacc agggcagtgc tgaactgtgt
2461 ggctctcaag aggtagatca gcccacaagt ggcgcagaca cagggacttc cgatgcatca
2521 ggaggaagcc gaagagccgt cagtgagacc gagtctctcg atcagggctc tgagccttcc
2581 caggtgtcct ctgaaacaga acccagtgat cctgccagga cagagagtgt gagcgaagcc
2641 agcaccaggc ctgagggaga gagtgacctg gaatgcgctg acagctcctg caatgagagt
2701 gtgaccacgc agctgtcctc tgtggacaca cggtgctcct ctcttgagag cgcacggttt
2761 cctgaaaccc cagccttttc ttctcaggag gaggaagacg gagcctgtgc agcagagccc
2821 accagcagtg gccctgccga agggtcgcag gaatccgtgt gcactgctgg ttctttacct
2881 gtggtacagg tgcccagtgg ggaggatgaa gggccagggg cagaatcggc cactgtacct
2941 gaccaggagg agctggggga ggtctggcag cggaggggga gcctggaggg agctgcagct
3001 gctgccgaga gcccaccgca agaagaaggc agtgctgggg aggcccaagg cacctgtgaa
3061 ggggcaactg cccaggagga gggcgctact ggaggttctc aagccaacgg ccaccagcca
3121 ctgcgatcac taccttcagt gcgcaaggat gttagccggt accagagggt ggacgaggct
3181 ctcccaccaa actgggaggc acgcattgac agccacggca ggatcttcta cgtggatcac
3241 gtaaacagaa ccacgacgtg gcagcgaccg acagctcccc cagccccgca ggtgctgcag
3301 agatctaact ccatacagca aatggagcag ctgaaccggc gatatcagag tatccgcaga
3361 accatgacca atgagaggcc tgaggaaaat accaatgcta ttgatggggc aggggaggaa
3421 gctgactttc accaagccag tgcagatttc cgacgggaga acatcctgcc tcactctacc
3481 tccagatcca ggatcacgtt gctgttacag tctccccccg tgaagttcct catcagccca
3541 gagttcttca ccgtgctgca ttctaaccct agtgcctacc gcatgtttac aaacaacacg
3601 tgtttgaagc acatgatcac caaagtccgg agggacaccc accactttga acgctaccag
3661 cataaccgcg accttgtggg attcctcaac atgttcgcga acaaacagct agagctgccg
3721 tggggatggg aaatgaaaca tgatcaccag gtaagtatca aggttacaag acaggtttaa
3781 ggagaccaat aagctattat tcgtatagga tactttatac gaagttatta agttatggag
3841 accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
3901 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
3961 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
4021 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
4081 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
4141 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
4201 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
4261 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
4321 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
4381 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
4441 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
4501 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
4561 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
4621 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
4681 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
4741 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
4801 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
4861 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
4921 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
4981 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
5041 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
5101 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
5161 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
5221 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
5281 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
5341 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
5401 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
5461 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
5521 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
5581 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
5641 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
5701 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
5761 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
5821 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
5881 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
5941 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
6001 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
6061 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
6121 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
6181 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
6241 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
6301 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
6361 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
6421 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
6481 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
6541 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
6601 gctcacatgt
//
