| 质粒名称: | pAAVLINK-EF1a-HA-5'-DIP2C |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001497 |
| 产品用途: | 5' AAVLINK plasmid for DIP2C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
EF1a
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DIP2C |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-DIP2C
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-DIP2C载体质粒基本信息
pAAVLINK-EF1a-HA-5'-DIP2C质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-DIP2C载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-DIP2C质粒载体简介
pAAVLINK-EF1a-HA-5'-DIP2C质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-DIP2C 6163 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6163)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6163
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3325
/codon_start=1
/label=DIP2C
/translation="ADRSLEGMALPLEVRARLAELELELSEGDITQKGYEKKRSKLIGA
YLPQPPRVDQALPQERRAPVTPSSASRYHRRRSSGSRDERYRSDVHTEAVQAALAKHKE
RKMAVPMPSKRRSLVVQTSMDAYTPPDTSSGSEDEGSVQGDSQGTPTSSQGSINMEHWI
SQAIHGSTTSTTSSSSTQSGGSGAAHRLADVMAQTHIENHSAPPDVTTYTSEHSIQVER
PQGSTGSRTAPKYGNAELMETGDGVPVSSRVSAKIQQLVNTLKRPKRPPLREFFVDDFE
ELLEVQQPDPNQPKPEGAQMLAMRGEQLGVVTNWPPSLEAALQRWGTISPKAPCLTTMD
TNGKPLYILTYGKLWTRSMKVAYSILHKLGTKQEPMVRPGDRVALVFPNNDPAAFMAAF
YGCLLAEVVPVPIEVPLTRKDAGSQQIGFLLGSCGVTVALTSDACHKGLPKSPTGEIPQ
FKGWPKLLWFVTESKHLSKPPRDWFPHIKDANNDTAYIEYKTCKDGSVLGVTVTRTALL
THCQALTQACGYTEAETIVNVLDFKKDVGLWHGILTSVMNMMHVISIPYSLMKVNPLSW
IQKVCQYKAKVACVKSRDMHWALVAHRDQRDINLSSLRMLIVADGANPWSISSCDAFLN
VFQSKGLRQEVICPCASSPEALTVAIRRPTDDSNQPPGRGVLSMHGLTYGVIRVDSEEK
LSVLTVQDVGLVMPGAIMCSVKPDGVPQLCRTDEIGELCVCAVATGTSYYGLSGMTKNT
FEVFPMTSSGAPISEYPFIRTGLLGFVGPGGLVFVVGKMDGLMVVSGRRHNADDIVATA
LAVEPMKFVYRGRIAVFSVTVLHDERIVIVAEQRPDSTEEDSFQWMSRVLQ"
misc_feature 1260..1265
/label=SDS
misc_feature 1352..1357
/label=SDS
misc_feature 2667..2672
/label=SDS
intron 3326..3366
/label=Splicing Donor
misc_feature 3370..3403
/label=lox2272-JT1571
repeat_region 3448..3566
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3448..3555
/label=AAV2 ITR
rep_origin 3641..4096
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4378..4482
/gene="bla"
/label=AmpR promoter
CDS 4483..5343
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5514..6102
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgcgg accgcagcct ggagggcatg gcgctgcccc tggaggtgcg
781 ggcgcgcctg gccgagctgg agctggagct gtcggaaggt gacatcacac aaaaaggata
841 tgaaaagaag aggtcaaagt taattggagc ctaccttccg cagcctccga gggtggacca
901 agctttgccg caagaacgcc gggctcctgt cactccttcc tccgcctctc gctaccaccg
961 ccgacggtct tcagggtcac gagatgagcg ctatcggtca gacgtccaca cggaagctgt
1021 ccaggcggct ctggccaaac acaaagagcg gaagatggca gtgcctatgc cttccaaacg
1081 caggtccctg gtcgtgcaga cctcgatgga cgcctacacc cctccagata cctcttctgg
1141 ctcagaagat gaaggctcag tgcaggggga ctcccagggc acccccacct ccagccaggg
1201 cagcatcaat atggagcact ggatcagcca ggccatccac ggctccacca cgtccaccac
1261 ctcctcgtcc tctacgcaga gcgggggcag cggggctgcc cacaggctgg cggacgtcat
1321 ggctcagacc cacatagaaa atcattctgc acctcctgac gtaaccacgt acacctcaga
1381 gcactcgata caggtggaga gaccgcaggg ttccacgggg tcccggacag cgcccaagta
1441 cggcaacgcc gagctcatgg agaccgggga tggagtacca gtaagtagcc gggtgtcagc
1501 aaaaatccag cagcttgtca ataccctcaa acgaccgaaa cgaccacctt tacgagaatt
1561 ctttgtcgat gactttgaag aattattaga agttcaacaa ccggatccga accaaccaaa
1621 gccggagggg gcccagatgc tggccatgcg cggagagcag ctgggcgtgg tcacgaactg
1681 gccgccgtcg ctggaggccg cactgcagag gtggggcacc atctcgccca aggcgccctg
1741 cctgaccacc atggacacca acgggaagcc cctctacatc ctcacttacg gcaagctgtg
1801 gacaagaagt atgaaggtcg cttacagcat tctacacaaa ttaggcacaa agcaggaacc
1861 catggtccgg cctggagata gggtggcact ggtgttcccc aacaatgatc cggctgcctt
1921 catggcggct ttctacggct gcctgctggc cgaggtggtc cccgtgccca tcgaggtgcc
1981 actcaccagg aaggacgcag ggagccagca gataggtttc ttgcttggaa gctgtggagt
2041 tactgtagcc ttgactagtg acgcctgcca taaaggactt ccaaaaagcc caacgggaga
2101 gatcccacag tttaaaggtt ggccaaagct gctgtggttt gtcacagagt ctaaacatct
2161 ctccaaaccg ccccgagact ggttcccaca cattaaagat gccaataacg acactgcgta
2221 tattgagtac aagacgtgta aggatggcag tgtgctgggt gtgacggtga cgaggactgc
2281 gctgctgaca cactgccagg ccctgacgca ggcgtgtggc tacacggaag ctgaaaccat
2341 tgtgaatgtg ctggatttca agaaggacgt cgggctctgg catggcatcc tgacaagcgt
2401 catgaacatg atgcatgtga tcagcatccc gtactcgctg atgaaggtga accctctctc
2461 ctggatccag aaggtctgcc agtacaaagc aaaagtggcg tgtgtgaaat cgagggatat
2521 gcattgggca ttagtagcac acagagatca gagagacatc aacctctcct ctctgcgaat
2581 gctgatagtg gcggacggcg cgaacccctg gtctatttct tcttgcgatg catttctcaa
2641 tgtcttccaa agtaaaggcc ttcgacagga ggtcatctgt ccttgtgcca gctcgccaga
2701 ggccctcact gtggccatcc ggaggcccac ggatgacagt aaccagcccc cgggccgggg
2761 tgtcctctcc atgcatggac tgacctatgg ggtcattcgt gtggactcgg aagagaagct
2821 gtccgtgctc accgtgcagg atgtcggcct cgtgatgcct ggagccatca tgtgttcagt
2881 gaagccagac ggggttcctc agctgtgcag aacggatgag atcggggagc tgtgtgtgtg
2941 tgcagttgcg acgggcacgt cctactatgg cctctctggc atgaccaaga acacctttga
3001 ggtgtttccc atgacaagct ccggggctcc gatcagtgaa tacccattca taaggacagg
3061 cttgctgggg ttcgtgggtc ccggaggcct cgtcttcgtg gtgggcaaga tggatggcct
3121 catggtggtc agcgggcgca ggcacaacgc cgacgacatc gtggccactg cgctggccgt
3181 agaacccatg aagtttgtct accggggaag gatagccgtg ttctcggtga ccgtgctgca
3241 cgacgagagg atcgtgatcg tggctgagca gaggcctgac tccacggaag aggacagttt
3301 ccagtggatg agccgtgtgc tgcaggtaag tatcaaggtt acaagacagg tttaaggaga
3361 ccaataagct attattcgta taggatactt tatacgaagt tattaagtta tggagaccgg
3421 tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
3481 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccgg cctcagtgag
3541 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
3601 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
3661 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
3721 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
3781 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
3841 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
3901 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
3961 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
4021 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
4081 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
4141 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
4201 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
4261 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
4321 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
4381 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
4441 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
4501 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
4561 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
4621 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
4681 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
4741 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
4801 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
4861 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
4921 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
4981 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
5041 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
5101 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
5161 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
5221 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
5281 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
5341 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
5401 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
5461 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
5521 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
5581 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
5641 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
5701 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
5761 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
5821 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
5881 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
5941 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
6001 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
6061 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
6121 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
