| 质粒名称: | pAAVLINK-EF1a-HA-5'-CLTCL1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001512 |
| 产品用途: | 5' AAVLINK plasmid for CLTCL1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CLTCL1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-CLTCL1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CLTCL1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CLTCL1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CLTCL1质粒载体简介
pAAVLINK-EF1a-HA-5'-CLTCL1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CLTCL1 6835 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6835)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6835
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3997
/codon_start=1
/label=CLTCL1
/translation="MAQILPVRFQEHFQLQNLGINPANIGFSTLTMESDKFICIREKVG
EQAQVTIIDMSDPMAPIRRPISAESAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTM
AEEVIFWKWVSVNTVALVTETAVYHWSMEGDSQPMKMFDRHTSLVGCQVIHYRTDEYQK
WLLLVGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFAEFKMEGNAKPATLFCFAVRN
PTGGKLHIIEVGQPAAGNQPFVKKAVDVFFPPEAQNDFPVAMQIGAKHGVIYLITKYGY
LHLYDLESGVCICMNRISADTIFVTAPHKPTSGIIGVNKKGQVLSVCVEEDNIVNYATN
VLQNPDLGLRLAVRSNLAGAEKLFVRKFNTLFAQGSYAEAAKVAASAPKGILRTRETVQ
KFQSIPAQSGQASPLLQYFGILLDQGQLNKLESLELCHLVLQQGRKQLLEKWLKEDKLE
CSEELGDLVKTTDPMLALSVYLRANVPSKVIQCFAETGQFQKIVLYAKKVGYTPDWIFL
LRGVMKISPEQGLQFSRMLVQDEEPLANISQIVDIFMENSLIQQCTSFLLDALKNNRPA
EGLLQTWLLEMNLVHAPQVADAILGNKMFTHYDRAHIAQLCEKAGLLQQALEHYTDLYD
IKRAVVHTHLLNPEWLVNFFGSLSVEDSVECLHAMLSANIRQNLQLCVQVASKYHEQLG
TQALVELFESFKSYKGLFYFLGSIVNFSQDPDVHLKYIQAACKTGQIKEVERICRESSC
YNPERVKNFLKEAKLTDQLPLIIVCDRFGFVHDLVLYLYRNNLQRYIEIYVQKVNPSRT
PAVIGGLLDVDCSEEVIKHLIMAVRGQFSTDELVAEVEKRNRLKLLLPWLESQIQEGCE
EPATHNALAKIYIDSNNSPECFLRENAYYDSSVVGRYCEKRDPHLACVAYERGQCDLEL
IKVCNENSLFKSEARYLVCRKDPELWAHVLEETNPSRRQLIDQVVQTALSETRDPEEIS
VTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYISRLD
NYDALDIASIAVSSALYEEAFTVFHKFDMNASAIQ"
misc_feature 1420..1425
/label=SDS
misc_feature 2611..2616
/label=SDS
misc_feature 2680..2685
/label=SDS
misc_feature 2973..2978
/label=SDS
misc_feature 3039..3044
/label=SDS
misc_feature 3945..3950
/label=SDS
intron 3998..4038
/label=Splicing Donor
misc_feature 4042..4075
/label=lox2272-JT1571
repeat_region 4120..4238
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4120..4227
/label=AAV2 ITR
rep_origin 4313..4768
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5050..5154
/gene="bla"
/label=AmpR promoter
CDS 5155..6015
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6186..6774
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggcgcagatc ctccctgttc gctttcagga
781 gcacttccag ctccaaaacc ttggaattaa tccagctaac attggattca gcacactgac
841 catggaatct gacaagttca tatgtatccg agagaaagtt ggtgagcagg cacaggtcac
901 gatcattgac atgagtgacc caatggctcc gatccgacgg cctatctctg cagagagtgc
961 catcatgaat ccagcctcta aggtgatagc tctgaaagct gggaagacac ttcagatctt
1021 taatattgag atgaagagta aaatgaaggc tcatactatg gcagaagaag tgattttctg
1081 gaaatgggtt tctgtgaaca ctgttgcctt ggtgaccgag accgcggtct accactggag
1141 catggaaggt gactcccagc ccatgaagat gtttgataga cataccagtc tggtgggctg
1201 ccaggtgatt cactaccgga ctgatgagta ccagaagtgg ctgctgctcg taggcatctc
1261 ggctcagcaa aaccgtgtgg ttggagcaat gcagctctac tctgtggata ggaaggtttc
1321 acaacccata gaaggccatg ctgcggcttt tgcagagttc aagatggagg ggaatgccaa
1381 gcctgccacc cttttctgct ttgctgtacg taatcccaca ggaggcaagt tgcacatcat
1441 tgaagttgga cagcctgcag cgggaaacca accttttgta aagaaagcag tagatgtgtt
1501 ttttcctcca gaggcacaga atgattttcc agtggctatg cagattggag ctaaacatgg
1561 tgttatttac ttgatcacaa agtatggcta tcttcatctg tacgacctag agtctggcgt
1621 gtgcatctgc atgaaccgta ttagtgctga cacaatattt gtcactgctc cacacaaacc
1681 aacctctgga attattggtg tcaacaaaaa gggacaggta ctgtcagttt gtgttgagga
1741 agataacatt gtgaattatg caaccaacgt gcttcagaat ccagaccttg gtctgcgttt
1801 ggccgttcgt agtaacctgg ctggggcaga gaagttgttt gtgagaaaat tcaataccct
1861 ctttgcacag ggcagctatg ctgaagccgc caaagttgca gcgtctgcac caaagggaat
1921 cctgcgtacc agagagacgg tccagaaatt ccagagtata cccgctcagt ctggccaggc
1981 ttctccattg ctgcagtact tcggaatcct gctcgaccag ggtcagctca ataaacttga
2041 atccttagaa ctttgccatc tggttcttca gcaggggcgt aagcaactcc tagagaagtg
2101 gctgaaagaa gataagctgg agtgctcaga ggagctcgga gacttggtca aaaccactga
2161 ccccatgctc gctctgagtg tgtaccttcg ggcaaatgtg ccaagcaaag tgatccagtg
2221 ttttgcagaa acaggccaat tccagaaaat tgtgctctat gccaaaaagg ttgggtacac
2281 cccagactgg atctttctgc tgaggggtgt aatgaagatc agtccggaac agggcctgca
2341 gttttctcga atgctagtgc aggacgagga gccgctggcc aacattagcc agattgtgga
2401 cattttcatg gaaaacagtt taattcagca gtgtacttcc ttcttattgg atgccttgaa
2461 gaataatcgc ccagctgagg gactcctgca gacatggctg ttggagatga accttgttca
2521 tgcaccccag gttgcagatg ccatccttgg aaataaaatg tttactcatt acgaccgggc
2581 ccacattgcc cagctctgtg agaaggcagg cctcctgcag caagcactgg agcactacac
2641 cgacctctat gacatcaaga gggctgtggt ccacactcac ctcctcaatc ccgagtggct
2701 tgtcaatttc tttggctcct tatcggtgga ggattctgtg gagtgtctgc atgccatgct
2761 gtctgctaac atcagacaga accttcagct gtgtgtgcag gtggcctcta agtaccacga
2821 gcagctgggc acgcaggccc tggtggagct ctttgaatcc ttcaagagtt acaaaggcct
2881 cttctacttc ctgggctcaa tcgtgaactt cagccaagac ccagatgtgc atctgaaata
2941 cattcaggct gcctgtaaga cagggcagat caaggaggtg gagaggatat gccgagagag
3001 cagctgctac aacccagagc gtgtgaagaa cttcctgaag gaggccaagc tcacagacca
3061 gcttcccctc atcatcgtgt gtgatcgttt tggctttgtc catgaccttg tcctatattt
3121 ataccgcaac aacctgcaga ggtacattga gatctacgtg cagaaggtca accctagccg
3181 gaccccagct gtgattggag ggctgcttga tgtggattgt tctgaggaag tgattaaaca
3241 cttaatcatg gcagtgagag gacagttctc tactgatgag ttggtggctg aagtagaaaa
3301 aagaaatagg ctcaagctgc tgcttccctg gctggagtcc cagattcagg aaggctgtga
3361 ggagcctgcc actcacaatg cactggctaa aatctacatc gacagcaaca acagccccga
3421 gtgcttcctg agagagaatg cctactatga cagcagcgtg gtgggccgct actgtgagaa
3481 gcgagacccc catctggcct gtgttgccta tgagcggggg cagtgtgacc ttgagctcat
3541 caaggtgtgc aatgagaatt ctctgttcaa aagcgaggcc cgctacctgg tatgcagaaa
3601 ggatccggag ctctgggctc acgtccttga ggagaccaac ccatccagga gacagctaat
3661 tgaccaggtg gtacagacag cattgtcaga aacacgggat cctgaagaga tttcggtcac
3721 tgtcaaagcc tttatgacag ccgacctgcc taatgaactg attgaactgc tggagaagat
3781 agttctggat aactctgtct tcagcgagca caggaatcta cagaatctgt tgatcctgac
3841 tgccatcaag gcagaccgca cacgggtcat ggagtacatc agccgcctgg acaactatga
3901 cgcactggac atcgcgagca tcgctgtcag cagcgcactg tatgaggagg ccttcaccgt
3961 tttccacaag tttgatatga atgcctcagc aatccaggta agtatcaagg ttacaagaca
4021 ggtttaagga gaccaataag ctattattcg tataggatac tttatacgaa gttattaagt
4081 tatggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
4141 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
4201 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
4261 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
4321 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
4381 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
4441 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
4501 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
4561 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
4621 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
4681 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
4741 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
4801 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
4861 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
4921 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
4981 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
5041 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
5101 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
5161 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
5221 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
5281 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
5341 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
5401 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
5461 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
5521 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
5581 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
5641 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
5701 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
5761 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
5821 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
5881 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
5941 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
6001 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
6061 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
6121 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
6181 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
6241 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
6301 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
6361 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
6421 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
6481 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
6541 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
6601 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
6661 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
6721 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
6781 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
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