| 质粒名称: | pAAVLINK-EF1a-HA-5'-CLASP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001494 |
| 产品用途: | 5' AAVLINK plasmid for CLASP1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CLASP1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-CLASP1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-CLASP1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CLASP1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CLASP1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CLASP1质粒载体简介
pAAVLINK-EF1a-HA-5'-CLASP1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CLASP1 6294 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6294)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6294
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3434
/codon_start=1
/label=CLASP1
/translation="MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDKQKSADLEHDQT
MLDKLVDGLATSWVNSSNYKVVLLGMDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAK
DSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKNFRTREGICLCLIATLNASGAQTL
TLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKGLPQSRLNVIFTK
FDEVQKSGNMIQSANDKNFDDEDSVDGNRPSSASSTSSKAPPSSRRNVGMGTTRRLGSS
TLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQ
RVNALKKIRSLLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVL
GNKFDHGAEAIMPTIFNLIPNSAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSV
AVRRRCFEFLDLLLQEWQTHSLERHISVLAETIKKGIHDADSEARIEARKCYWGFHSHF
SREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQESLNRPLSAKRSPTGS
TTSRASTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGTTPFSSAAALPPGSYA
SLGRIRTRRQSSGSATNVASTPDNRGRSRAKVVSQSQRSRSANPAGAGSRSSSPGKLLG
SGYGGLTGGSSRGPPVTPSSEKRSKIPRSQGCSRETSPNRIGLARSSRIPRPSMSQGCS
RDTSRESSRDTSPARGFPPLASRRHSRSTSALSTAESVGQSDRFGLGQPGRIPGSVNAM
RVLSTSTDLEAAVADALKKPVRRRYEPYGMYSDDDANSDASSVCSERSYGSRNGGIPHY
LRQTEDVAEVLNHCASSNWSERKE"
misc_feature 2337..2342
/label=SDS
misc_feature 3248..3253
/label=SDS
intron 3435..3475
/label=Splicing Donor
misc_feature 3479..3512
/label=lox2272-JT1571
repeat_region 3557..3697
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3557..3686
/label=AAV2 ITR
rep_origin 3772..4227
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4509..4613
/gene="bla"
/label=AmpR promoter
CDS 4614..5474
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5645..6233
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggagcctcgc atggagtcct gcctggcgca
781 ggtgttgcag aaggatgtgg ggaaacgatt gcaggttggc caagaactga tagactattt
841 ctcagacaaa cagaagtctg ctgaccttga gcatgaccag accatgttag ataaacttgt
901 ggatggactt gctacctctt gggtgaactc tagcaattac aaggtggttc tgctgggcat
961 ggacatcctg tccgccctgg tgacccggct gcaggatcgg ttcaaggcgc agatcggcac
1021 agtgctgcca agtctaatag acagactagg agatgctaaa gactctgtga gggagcagga
1081 ccaaactctg ctgctaaaga tcatggatca agctgctaat ccccagtacg tatgggacag
1141 aatgcttgga ggcttcaaac acaagaattt ccgtactcga gaaggcatct gtctctgcct
1201 tatagcaaca ctcaatgcct ctggagcaca gactttaaca ctaagcaaga ttgtgccaca
1261 tatatgcaac ttacttggag atccaaacag ccaggttcga gatgcagcaa taaacagctt
1321 agtggaaatt tacagacatg taggagaacg tgtgagggca gatctcagta aaaaaggatt
1381 gccacagtcc cggttgaatg taatttttac aaaatttgat gaagtccaga aatctggaaa
1441 catgatacaa tctgcaaatg ataaaaattt cgacgatgaa gattctgtgg atggtaacag
1501 accttcctct gctagttcta catcatccaa ggctccacca agttctcgga gaaacgttgg
1561 aatgggaacc acccgccggc ttggttcatc cacccttgga tccaagtctt cagctgcaaa
1621 agaaggagct ggtgctgttg atgaagagga ttttattaaa gcatttgatg atgtacctgt
1681 agtacagatt tattccagcc gagaccttga ggaatccata aacaaaatta gggaaatatt
1741 atctgatgac aagcatgatt gggagcagag agtaaatgct ctaaaaaaga ttagatcttt
1801 acttttggct ggtgctgctg agtatgataa cttctttcaa catttgcgtc ttttggatgg
1861 agcctttaaa ctctctgcta aggacctgcg gtctcaggta gtgcgggagg cttgtatcac
1921 gttggggcat ctgtcatcag ttctggggaa taagtttgac catggagctg aagccattat
1981 gccaactatc tttaatttaa ttccaaacag tgccaaaatt atggccacat ctggtgttgt
2041 agctgttagg ttaattattc ggcacacaca catccctagg ttaatacctg tcataacaag
2101 caactgtacc tctaagtctg tcgcagttag aaggcgctgt tttgaatttt tagatttgct
2161 tttacaagaa tggcagacac attcactaga acgacacata tcagtattag ctgaaacaat
2221 aaagaaggga atacatgatg ctgattccga agcaagaata gaagccagaa aatgttactg
2281 gggtttccac agtcacttca gcagagaagc agagcacttg taccacacct tggagtcctc
2341 ctaccagaaa gccctgcagt cccacctgaa gaactcagac agcatagtgt ctctgcctca
2401 gtcagaccgc tcatcttcca gctctcaaga gagtctaaat cgtccgctgt ctgccaaaag
2461 aagtcctact ggaagtacca catctagagc ttctacagtt agtaccaaat ctgtgtcaac
2521 gactgggtcc ctccagcgat ctcgaagtga tattgatgtg aacgcagcag ccagtgccaa
2581 atccaaagtc tcctcatctt cgggcacgac gcctttcagc tctgcagcag ctttgcctcc
2641 agggtcatac gcatccttag gtcggatccg cacaagacgg caaagctctg ggagtgccac
2701 caacgtcgcc tctacacctg ataaccgggg ccgcagtcgc gctaaagtgg tttcacagtc
2761 ccagcgatcc agatctgcta atcctgctgg tgctggcagc cggtcaagtt ccccaggaaa
2821 attgttggga agtggttatg gtggacttac tgggggctcc tcacgaggcc cacctgtgac
2881 accgtcttca gaaaagcgaa gcaagattcc caggagccag ggatgtagcc gggaaacaag
2941 tccaaaccga ataggattag cacggagcag ccgtatccct cgacccagca tgagtcaggg
3001 gtgcagccgc gataccagcc gtgagagcag ccgagataca agccctgctc ggggctttcc
3061 tccacttgcc tctcgacgtc attccaggtc cactagtgct ctctccactg ctgaatctgt
3121 tgggcagtca gatcggtttg ggcttggcca gccaggaaga atacctggtt ctgtgaatgc
3181 catgagagtt ctgagcacaa gtacagatct tgaagctgct gttgctgatg ctttgaagaa
3241 gcctgtgagg aggagatatg agccgtatgg gatgtattct gacgatgatg ccaacagtga
3301 tgcctcaagt gtttgctctg agcgctcata tggctccagg aatggtggca ttccccatta
3361 tctgcggcag actgaggatg tagcagaagt tctcaaccac tgtgctagtt caaactggtc
3421 agaaaggaaa gaaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta
3481 ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt gtggcacgtg
3541 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
3601 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
3661 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
3721 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
3781 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3841 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
3901 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
3961 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
4021 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4081 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
4141 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4201 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
4261 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
4321 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
4381 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
4441 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
4501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4561 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
4621 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
4681 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
4741 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
4801 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
4861 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
4921 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
4981 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
5041 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
5101 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
5161 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
5221 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
5281 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
5341 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
5401 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
5461 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
5521 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
5581 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
5641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
5701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
5761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
5821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
5881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
5941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
6001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
6061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
6121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
6181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
6241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
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