| 质粒名称: | pAAVLINK-EF1a-HA-5'-CHD1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001539 |
| 产品用途: | 5' AAVLINK plasmid for CHD1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-CHD1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-CHD1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CHD1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CHD1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CHD1质粒载体简介
pAAVLINK-EF1a-HA-5'-CHD1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CHD1 6422 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6422)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6422
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3584
/codon_start=1
/label=CHD1
/translation="NGHSDEESVRNSSGESSQSDDDSGSASGSGSGSSSGSSSDGSSSQ
SGSSDSDSGSESGSQSESESDTSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAILKKQ
QQQQQQQQHQASSNSGSEEDSSSSEDSDDSSSEVKRKKHKDEDWQMSGSGSPSQSGSDS
ESEEEREKSSCDETESDYEPKNKVKSRKPQNRSKSKNGKKILGQKKRQIDSSEEDDDEE
DYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEEEEFETIERFMDCRI
GRKGATGATTTIYAVEADGDPNAGFEKNKEPGEIQYLIKWKGWSHIHNTWETEETLKQQ
NVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQ
KSAAGYPDYYCKWQGLPYSECSWEDGALISKKFQACIDEYFSRNQSKTTPFKDCKVLKQ
RPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTIS
FLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWT
HHQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSN
HRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLL
RRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMME
LKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQM
VRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGIN
LASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMV
LDHLVIQRMDTTGKTVLHT"
misc_feature 1383..1388
/label=SDS
misc_feature 1461..1466
/label=SDS
misc_feature 3063..3068
/label=SDS
intron 3585..3625
/label=Splicing Donor
misc_feature 3629..3662
/label=lox2272-JT1571
repeat_region 3707..3825
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3707..3814
/label=AAV2 ITR
rep_origin 3900..4355
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4637..4741
/gene="bla"
/label=AmpR promoter
CDS 4742..5602
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5773..6361
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctaatg gacacagtga tgaagaaagt gttagaaaca gtagtggaga
781 atcaagccag tcggatgatg attctgggtc agcttcaggc tctggatctg gttcgagttc
841 tggaagcagt agtgatggaa gcagtagcca gtcaggtagc agtgactctg actccggatc
901 tgaatcaggc agtcagtcag agtctgagtc agacacttcc cgagaaaaca aagttcaagc
961 aaaaccaccg aaagttgatg gagctgagtt ttggaaatct agtcctagta ttctggccgt
1021 tcagagatct gcaatcctca agaagcagca acagcagcag cagcaacaac aacatcaagc
1081 ctcatctaat agcggatcag aagaggattc ctctagcagt gaagattccg atgactcatc
1141 aagtgaggtc aaaaggaaaa agcataaaga tgaagattgg caaatgtctg ggtcaggatc
1201 tccatctcag tctggttcag attcagaatc tgaagaagag agagagaaaa gcagttgtga
1261 tgaaacagaa tctgattatg agccaaaaaa caaagtcaaa agcagaaaac ctcaaaatag
1321 atctaagtca aaaaatggaa agaagattct tggacaaaaa aagagacaga ttgattcatc
1381 tgaggaggat gatgatgaag aagattatga taatgataaa agaagttctc gtcgccaagc
1441 aactgttaat gttagctata aggaggatga agaaatgaaa acagattctg atgacctact
1501 ggaagtctgt ggagaggatg ttcctcaacc tgaggaagag gaatttgaaa ccatagaaag
1561 atttatggat tgtcggattg ggagaaaagg agctactggt gctactacaa ccatctatgc
1621 agttgaagca gatggtgacc caaatgcagg ctttgaaaaa aacaaagaac caggagagat
1681 tcagtattta attaaatgga aaggatggtc ccatatccac aacacttggg agacagaaga
1741 aaccctcaag cagcagaatg ttagaggaat gaaaaaattg gataattata agaaaaaaga
1801 tcaggaaaca aaaagatggt tgaaaaatgc ctctccagaa gatgtggaat attataattg
1861 ccagcaagaa cttacagatg atctacataa acagtatcaa atagtggaac gtataattgc
1921 tcattccaat caaaagtcag cagctggtta tcctgattat tactgcaaat ggcagggcct
1981 tccatactca gagtgcagct gggaagatgg agctctcatt tccaaaaagt ttcaagcatg
2041 cattgatgag tattttagca ggaaccaatc aaaaaccact ccttttaaag attgcaaagt
2101 attaaaacaa aggccaaggt ttgtagccct gaagaagcag ccatcctata ttggaggaca
2161 tgagggctta gaattaagag attatcaact gaatggttta aattggcttg ctcattcttg
2221 gtgcaaagga aatagttgca tactcgctga tgaaatgggc cttggaaaaa caatacagac
2281 gatctcattt ctgaattatt tgtttcatga acatcaatta tatggacctt ttttattggt
2341 agtaccgctc tccactctta cttcctggca aagggaaatt cagacttggg cttctcaaat
2401 gaatgctgtg gtttatttag gtgacattaa cagcagaaac atgataagaa ctcatgaatg
2461 gacgcatcat cagaccaaac ggttaaaatt taatatattg ttaacaactt atgaaatttt
2521 attaaaagat aaggcattcc ttggaggtct aaattgggca tttataggtg ttgatgaagc
2581 acaccgatta aagaatgatg actcccttct gtataaaact ttaatagatt ttaaatccaa
2641 tcatcgtctc cttatcactg gaactcctct acagaattcc ctcaaagagc tctggtcttt
2701 gctacatttc attatgccag aaaagttttc ttcctgggaa gattttgaag aagaacatgg
2761 caaagggaga gaatatggtt atgcaagcct tcacaaggag cttgagccat ttctgttacg
2821 ccgagttaag aaagatgtgg aaaaatctct tcctgccaag gttgagcaga ttttaagaat
2881 ggaaatgagt gctttacaga aacaatatta caaatggatt ttaactagga attacaaagc
2941 cctcagcaaa ggttccaagg gcagtacctc aggctttttg aacattatga tggagctaaa
3001 gaaatgttgt aaccattgct acctcattaa accaccagat aataatgaat tctataataa
3061 acaggaggcc ttacaacact taattcgtag tagcggaaaa ttgattcttc ttgacaagct
3121 attaattcgc ctaagagaac gaggcaatcg agttcttatt ttttcacaaa tggtgcggat
3181 gttagatata cttgcagaat atttgaaata tcgtcaattc ccctttcaaa gattagatgg
3241 atcaataaaa ggagaactga ggaaacaagc tctagatcat tttaatgctg agggatcaga
3301 ggatttttgc tttttgctgt ccacaagagc tggaggtcta gggattaatt tagcctctgc
3361 tgacactgtt gttatatttg attccgattg gaatccacag aatgatcttc aggcacaggc
3421 tagagcccat cgaattgggc aaaagaaaca ggtgaatatt tatcgtctag ttacaaaggg
3481 atcagttgaa gaagatattc ttgaaagggc gaaaaagaag atggttttag atcatcttgt
3541 aattcaaaga atggacacaa ctgggaagac agtactacat acaggtaagt atcaaggtta
3601 caagacaggt ttaaggagac caataagcta ttattcgtat aggatacttt atacgaagtt
3661 attaagttat ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg
3721 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
3781 gtcgcccggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
3841 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
3901 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
3961 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
4021 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
4081 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
4141 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
4201 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
4261 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
4321 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
4381 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
4441 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
4501 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
4561 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
4621 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
4681 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
4741 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
4801 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
4861 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
4921 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
4981 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
5041 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
5101 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
5161 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
5221 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
5281 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
5341 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
5401 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
5461 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
5521 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
5581 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
5641 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
5701 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
5761 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
5821 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
5881 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
5941 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
6001 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
6061 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
6121 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
6181 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
6241 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
6301 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
6361 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
6421 gt
//
