| 质粒名称: |
pAAVLINK-EF1a-HA-5'-CADPS
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001548 |
| 产品用途: | 5' AAVLINK plasmid for CADPS expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CADPS |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-CADPS
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-CADPS载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CADPS质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CADPS载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CADPS质粒载体简介
pAAVLINK-EF1a-HA-5'-CADPS质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CADPS 6030 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6030)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6030
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3170
/codon_start=1
/label=CADPS
/translation="MLDPSSSEEESDEIVEEESGKEVLGSAPSGARLSPSRTSEGSAGS
AGLGGGGAGAGAGVGAGGGGGSGASSGGGAGGLQPSSRAGGGRPSSPSPSVVSEKEKEE
LERLQKEEEERKKRLQLYVFVMRCIAYPFNAKQPTDMARRQQKISKQQLQTVKDRFQAF
LNGETQIMADEAFMNAVQSYYEVFLKSDRVARMVQSGGCSANDSREVFKKHIEKRVRSL
PEIDGLSKETVLSSWMAKFDAIYRGEEDPRKQQARMTASAASELILSKEQLYEMFQNIL
GIKKFEHQLLYNACQLDNPDEQAAQIRRELDGRLQMADQIARERKFPKFVSKEMENMYI
EELKSSVNLLMANLESMPVSKGGEFKLQKLKRSHNASIIDMGEESENQLSKSDVVLSFS
LEVVIMEVQGLKSLAPNRIVYCTMEVEGGEKLQTDQAEASKPTWGTQGDFSTTHALPAV
KVKLFTESTGVLALEDKELGRVILHPTPNSPKQSEWHKMTVSKNCPDQDLKIKLAVRMD
KPQNMKHSGYLWAIGKNVWKRWKKRFFVLVQVSQYTFAMCSYREKKAEPQELLQLDGYT
VDYTDPQPGLEGGRAFFNAVKEGDTVIFASDDEQDRILWVQAMYRATGQSHKPVPPTQV
QKLNAKGGNVPQLDAPISQFYADRAQKHGMDEFISSNPCNFDHASLFEMVQRLTLDHRL
NDSYSCLGWFSPGQVFVLDEYCARNGVRGCHRHLCYLRDLLERAENGAMIDPTLLHYSF
AFCASHVHGNRPDGIGTVTVEEKERFEEIKERLRVLLENQITHFRYCFPFGRPE"
misc_feature 795..800
/label=SDS
misc_feature 810..815
/label=SDS
misc_feature 1080..1085
/label=SDS
misc_feature 1083..1088
/label=SDS
misc_feature 2026..2031
/label=SDS
misc_feature 2538..2543
/label=SDS
intron 3171..3211
/label=Splicing Donor
misc_feature 3215..3248
/label=lox2272-JT1571
repeat_region 3293..3433
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3293..3422
/label=AAV2 ITR
rep_origin 3508..3963
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4245..4349
/gene="bla"
/label=AmpR promoter
CDS 4350..5210
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5381..5969
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gctggaccct tcgtccagcg aagaagaatc
781 ggatgagatc gtggaggagg agagcggcaa ggaggtgctc ggctcggccc cgtccggcgc
841 gcgcctgtct cccagccgta ccagcgaggg ctcggccggc agcgccgggc tggggggcgg
901 cggcgccggc gccggagccg gggtgggtgc aggcggcggc gggggcagcg gcgcgagcag
961 cggcggcggg gccggggggc tgcaacccag cagccgcgct ggcggcggcc ggccctccag
1021 ccccagcccg tcggtggtga gcgagaagga gaaggaagag ttggagcggc tgcagaaaga
1081 ggaggaggag aggaagaaga ggctgcagct gtatgtgttc gtgatgcgct gcatcgccta
1141 cccctttaat gccaagcagc ccaccgacat ggctcgccgg cagcagaaga tcagcaaaca
1201 gcagctgcag acagtcaagg accggtttca ggctttcctc aatggggaaa cccagatcat
1261 ggctgacgaa gccttcatga acgctgtgca gagttactat gaggtgttcc tgaagagcga
1321 ccgtgtggcc cgcatggttc agagtggagg ctgttccgcc aacgactccc gggaggtctt
1381 caagaagcac attgagaaga gagtgcgcag cctgcctgag attgacggcc tcagcaagga
1441 gactgtgctg agctcctgga tggccaaatt tgatgccatc taccgtggag aagaggaccc
1501 gcggaagcag caggcccgga tgacagccag cgcagcctcc gagctgattc tgagcaagga
1561 gcaactctat gagatgttcc agaacattct tgggatcaag aagttcgaac atcagctcct
1621 ttacaatgcc tgccagctgg acaatccaga tgagcaagca gcccagatca gacgagagct
1681 ggatggacgt ctacaaatgg cagaccaaat agccagggaa cgcaaatttc ccaagtttgt
1741 atccaaagaa atggaaaaca tgtacattga ggagctgaag tcatctgtca acctgctcat
1801 ggccaacttg gagagcatgc cggtatccaa aggcggggag ttcaagctcc agaaactcaa
1861 acgcagccac aatgcttcca tcatcgacat gggcgaggag agtgagaacc agctctccaa
1921 gtcagatgtc gtgctgtctt tctcattgga ggtggtaatt atggaagtcc aaggcctcaa
1981 atctttggct ccaaatcgca tcgtatattg cacaatggag gtggaaggag gagagaaact
2041 acagactgat caggccgagg cttctaaacc aacctggggc acccagggtg acttctccac
2101 aacccatgca ctgccagctg tgaaggtgaa gctgttcaca gagagcacag gcgtcctggc
2161 gttggaggac aaggagcttg ggcgggttat tctccatccc accccgaaca gccccaaaca
2221 gtcagagtgg cacaaaatga cagtctccaa aaactgcccc gaccaagatc tcaaaatcaa
2281 acttgctgtc cgaatggata agcctcaaaa catgaagcat tctgggtatt tatgggccat
2341 cggtaagaat gtctggaaga gatggaagaa aaggtttttt gtattggtgc aggtcagtca
2401 gtacacgttt gccatgtgca gttatcggga gaagaaagcg gagcctcagg aacttctaca
2461 attggatggc tacactgtgg attacaccga cccccagcca ggtttggagg gtggccgagc
2521 cttcttcaat gctgtcaagg agggagacac cgtgatattt gccagtgacg atgaacaaga
2581 ccgcatcctg tgggtccagg ccatgtatcg ggccacgggg cagtcacaca agcctgtgcc
2641 cccgacccaa gtccagaaac tcaacgccaa gggaggaaat gtacctcagc tggatgcccc
2701 tatctctcaa ttttacgcag atagagctca aaaacatggc atggatgaat ttatctcttc
2761 caacccctgt aactttgacc acgcttccct ctttgagatg gtacaacgcc ttactttgga
2821 tcacagactt aatgattcct attcttgcct gggctggttc agtcctggcc aggtgtttgt
2881 actagacgag tattgcgccc gaaatggagt ccgggggtgt caccgacatc tctgctacct
2941 cagagacttg cttgaacggg cagaaaatgg cgccatgatc gaccccaccc ttcttcacta
3001 cagctttgcc ttctgtgcat cccatgtcca tgggaacagg cctgatggaa ttggaactgt
3061 gactgttgaa gaaaaggaac gttttgaaga aatcaaagag aggctccgag ttctgctaga
3121 aaatcagatt acacatttta ggtattgctt tccatttggt cgacctgaag gtaagtatca
3181 aggttacaag acaggtttaa ggagaccaat aagctattat tcgtatagga tactttatac
3241 gaagttatta agttatggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
3301 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
3361 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
3421 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
3481 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
3541 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
3601 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
3661 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
3721 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
3781 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
3841 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
3901 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
3961 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
4021 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
4081 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
4141 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
4201 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
4261 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
4321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
4381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
4441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
4501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
4561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
4621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
4681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
4741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
4801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
4861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
4921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
4981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
5041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
5101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
5161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
5221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
5281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
5341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
5401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
5461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
5521 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
5581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
5641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
5701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
5761 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
5821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
5881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
5941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
6001 tggccttttg ctggcctttt gctcacatgt
//
