| 质粒名称: | pAAVLINK-EF1a-HA-5'-ATP10A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001485 |
| 产品用途: | 5' AAVLINK plasmid for ATP10A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
EF1a
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
ATP10A
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-ATP10A载体质粒基本信息
pAAVLINK-EF1a-HA-5'-ATP10A质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-ATP10A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-ATP10A质粒载体简介
pAAVLINK-EF1a-HA-5'-ATP10A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-ATP10A 5906 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5906)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5906
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3046
/codon_start=1
/label=ATP10A
/translation="MEREPAGTEEPGPPGRRRRREGRTRTVRSNLLPPPGAEDPAAGAA
KGERRRRRGCAQHLADNRLKTTKYTLLSFLPKNLFEQFHRPANVYFVFIALLNFVPAVN
AFQPGLALAPVLFILAITAFRDLWEDYSRHRSDHKINHLGCLVFSREEKKYVNRFWKEI
HVGDFVRLRCNEIFPADILLLSSSDPDGLCHIETANLDGETNLKRRQVVRGFSELVSEF
NPLTFTSVIECEKPNNDLSRFRGCIIHDNGKKAGLYKENLLLRGCTLRNTDAVVGIVIY
AGHETKALLNNSGPRYKRSKLERQMNCDVLWCVLLLVCMSLFSAVGHGLWIWRYQEKKS
LFYVPKSDGSSLSPVTAAVYSFLTMIIVLQVLIPISLYVSIEIVKACQVYFINQDMQLY
DEETDSQLQCRALNITEDLGQIQYIFSDKTGTLTENKMVFRRCTVSGVEYSHDANAQRL
ARYQEADSEEEEVVPRGGSVSQRGSIGSHQSVRVVHRTQSTKSHRRTGSRAEAKRASML
SKHTAFSSPMEKDITPDPKLLEKVSECDKSLAVARHQEHLLAHLSPELSDVFDFFIALT
ICNTVVVTSPDQPRTKVRVRFELKSPVKTIEDFLRRFTPSCLTSGCSSIGSLAANKSSH
KLGSSFPSTPSSDGMLLRLEERLGQPTSAIASNGYSSQADNWASELAQEQESERELRYE
AESPDEAALVYAARAYNCVLVERLHDQVSVELPHLGRLTFELLHTLGFDSVRKRMSVVI
RHPLTDEINVYTK"
misc_feature 2148..2153
/label=SDS
misc_feature 2151..2156
/label=SDS
misc_feature 2154..2159
/label=SDS
intron 3047..3087
/label=Splicing Donor
misc_feature 3091..3124
/label=lox2272-JT1571
repeat_region 3169..3309
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3169..3298
/label=AAV2 ITR
rep_origin 3384..3839
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4121..4225
/gene="bla"
/label=AmpR promoter
CDS 4226..5086
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5257..5845
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggagcgggag ccggcgggga ccgaggagcc
781 cgggcctccg ggacggcgga ggcgccgaga gggcaggacg cgcacggtgc gctccaacct
841 gctgccgccc ccgggcgccg aggaccctgc ggctggcgcg gccaagggcg agcggcgacg
901 gcggcgcggg tgtgcccagc acctggccga caaccggctc aagactacca agtacacgct
961 gctgtccttc ctgcccaaga acctgttcga gcagttccac cgcccggcca acgtgtactt
1021 tgtcttcatc gcgctgctca acttcgtgcc ggcggtgaac gccttccagc ccggcctggc
1081 actggcgccg gtgctcttca tcctggccat cacggccttc agggacctgt gggaggacta
1141 cagccgccac cgctccgacc acaagatcaa ccacctgggc tgcctggtct tcagcaggga
1201 agaaaagaaa tacgtgaacc gattctggaa agaaatccac gtgggagact ttgtgcgtct
1261 tcgctgcaac gaaatcttcc ctgcggacat tctgctgctc tcctccagtg accccgacgg
1321 gctatgccac atcgagaccg ccaacctgga tggagagacc aacctgaagc ggcggcaggt
1381 ggtccgcggc ttctcggagc ttgtctccga attcaatcct ttgacgttca ccagcgtgat
1441 cgaatgcgag aagccaaaca acgacctgag taggtttcgc ggctgcatca tacatgacaa
1501 cgggaaaaag gccgggctgt ataaagaaaa cctgctgctg aggggctgca cccttaggaa
1561 cacggacgca gtcgtcggca ttgtcatcta cgcaggacat gaaaccaagg ctctgctgaa
1621 caacagtggg ccccgctaca agcgcagcaa gctggagagg cagatgaact gcgacgtgct
1681 ctggtgtgtc ctgctccttg tttgcatgtc tctgttttca gcagtcggac atggactgtg
1741 gatatggcgg tatcaagaga agaagtcatt attttatgtc cccaagtctg atggaagctc
1801 cttatcccca gtcacagctg cagtttactc atttttaaca atgataatag ttctgcaggt
1861 tttgatccca atttccttat acgtttccat tgaaattgtt aaagcatgcc aagtgtactt
1921 cattaaccag gacatgcagt tgtatgacga agaaacagac tcgcagctgc agtgccgagc
1981 tctgaacatc acggaagact taggacagat acagtacatt ttctcagata aaactggcac
2041 tttgacagag aataagatgg ttttccgaag atgcactgtg tctggtgtag aatattctca
2101 tgatgcaaat gcgcagcgtc tggccaggta ccaagaggca gactcggagg aggaggaggt
2161 ggtgcccaga gggggctcgg tgtcccagcg cggcagcatc ggcagccacc agagtgtccg
2221 ggtggtgcac agaacccaga gcaccaagtc ccaccggcgc acgggcagcc gggccgaggc
2281 caagagggcc agcatgctgt ccaagcacac ggccttcagc agccccatgg agaaggatat
2341 cacgcccgac ccaaagctgc tggagaaggt gagtgagtgt gacaagagcc tagccgtggc
2401 gaggcatcag gagcacctgc tggcccacct ctcgcctgag ctgtctgacg tctttgattt
2461 cttcatcgca ctcaccatct gcaacacagt cgtcgtcacg tccccggatc agccacgaac
2521 aaaggtgagg gtgaggtttg agctgaagtc cccggtgaag acgatagaag acttcctgcg
2581 gaggttcaca cccagctgcc tgacctcagg ctgcagcagc atcgggagcc tggccgccaa
2641 caagtccagc cacaagttgg gctccagctt cccgtccacc ccgtccagcg acggcatgct
2701 tctcaggctg gaggagaggc tgggccagcc cacctcggcc atcgccagca acggctacag
2761 cagccaggcg gacaactggg cctcggagct tgctcaggag caggagtcag agcgcgagct
2821 gcggtacgag gcggagagcc cggatgaggc cgcactggtg tatgcggcca gagcctacaa
2881 ctgcgtgctt gtggagcggc tgcacgacca agtgtcagtg gagctgcccc acctgggcag
2941 gctcaccttc gagctcctgc acacactggg tttcgattcc gtccgcaaga ggatgtcagt
3001 ggtgatccgg cacccgctta ccgatgagat caacgtctac accaaggtaa gtatcaaggt
3061 tacaagacag gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag
3121 ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
3181 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
3241 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
3301 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
3361 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
3421 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
3481 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
3541 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
3601 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
3661 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
3721 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
3781 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
3841 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
3901 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
3961 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
4021 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
4081 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
4141 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
4201 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
4261 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
4321 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
4381 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
4441 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
4501 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
4561 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
4621 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
4681 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
4741 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
4801 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
4861 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
4921 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
4981 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
5041 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
5101 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
5161 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
5221 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
5281 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
5341 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
5401 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
5461 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
5521 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
5581 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
5641 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
5701 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
5761 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
5821 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
5881 cttttgctgg ccttttgctc acatgt
//
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pAAVLINK-EF1a-HA-5'-ATP10A质粒载体应用举例
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