| 质粒名称: | pAAVLINK-EF1a-HA-5'-ARHGAP5 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001488 |
| 产品用途: | 5' AAVLINK plasmid for ARHGAP5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ARHGAP5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-ARHGAP5
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-ARHGAP5载体质粒基本信息
pAAVLINK-EF1a-HA-5'-ARHGAP5质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-ARHGAP5载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-ARHGAP5质粒载体简介
pAAVLINK-EF1a-HA-5'-ARHGAP5质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-ARHGAP5 7218 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7218)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7218
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..4358
/codon_start=1
/label=ARHGAP5
/translation="MAKNKEPRPPSYTISVVGLSGTEKDKGNCGVGKSCLCNRFVRSKA
DEYYPEHTSVLSTIDFGGRVVNNDHFLYWGDIIQNSEDGVECKIHVIEQTEFIDDQTFL
PHRSTNLQPYIKRAAASKLQSAEKLMYICTDQLGLEQDFEQKQMPEGKLNVDGFLLCID
VSQGCNRKFDDQLKFVNNLFVQLSKSKKPVIIAATKCDECVDHYLREVQAFASNKKNLL
VVETSARFNVNIETCFTALVQMLDKTRSKPKIIPYLDAYKTQRQLVVTATDKFEKLVQT
VRDYHATWKTVSNKLKNHPDYEEYINLEGTRKARNTFSKHIEQLKQEHIRKRREEYINT
LPRAFNTLLPNLEEIEHLNWSEALKLMEKRADFQLCFVVLEKTPWDETDHIDKINDRRI
PFDLLSTLEAEKVYQNHVQHLISEKRRVEMKEKFKKTLEKIQFISPGQPWEEVMCFVME
DEAYKYITEADSKEVYGRHQREIVEKAKEEFQEMLFEHSELFYDLDLNATPSSDKMSEI
HTVLSEEPRYKALQKLAPDRESLLLKHIGFVYHPTKETCLSGQNCTDIKVEQLLASSLL
QLDHGRLRLYHDSTNIDKVNLFILGKDGLAQELANEIRTQSTDDEYALDGKIYELDLRP
VDAKSPYFLSQLWTAAFKPHGCFCVFNSIESLSFIGEFIGKIRTEASQIRKDKYMANLP
FTLILANQRDSISKNLPILRHQGQQLANKLQCPFVDVPAGTYPRKFNETQIKQALRGVL
ESVKHNLDVVSPIPANKDLSEADLRIVMCAMCGDPFSVDLILSPFLDSHSCSAAQAGQN
NSLMLDKIIGEKRRRIQITILSYHSSIGVRKDELVHGYILVYSAKRKASMGMLRAFLSE
VQDTIPVQLVAVTDSQADFFENEAIKELMTEGEHIATEITAKFTALYSLSQYHRQTEVF
TLFFSDVLEKKNMIENSYLSDNTRESTHQSEDVFLPSPRDCFPYNNYPDSDDDTEAPPP
YSPIGDDVQLLPTPSDRSRYRLDLEGNEYPIHSTPNCHDHERNHKVPPPIKPKPVVPKT
NVKKLDPNLLKTIEAGIGKNPRKQTSRVPLAHPEDMDPSDNYAEPIDTIFKQKGYSDEI
YVVPDDSQNRIKIRNSFVNNTQGDEENGFSDRTSKSHGERRPSKYKYKSKTLFSKAKSY
YRRTHSDASDDEAFTTSKTKRKGRHRGSEEDPLLSPVETWK"
misc_feature 1942..1947
/label=SDS
misc_feature 2009..2014
/label=SDS
misc_feature 3209..3214
/label=SDS
RBS 3697..3705
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
misc_feature 3698..3703
/label=SDS
intron 4359..4399
/label=Splicing Donor
misc_feature 4403..4436
/label=lox2272-JT1571
repeat_region 4481..4621
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4481..4610
/label=AAV2 ITR
rep_origin 4696..5151
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5433..5537
/gene="bla"
/label=AmpR promoter
CDS 5538..6398
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6569..7157
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctatgg caaaaaacaa agagcctcgt cccccatcct ataccatcag
781 tgtagttgga ctctctggga ctgaaaaaga caaaggtaac tgtggagttg gaaagtcttg
841 tttgtgcaat agatttgtac gctcaaaagc agatgaatat tatccagagc atacttctgt
901 gcttagcacc attgactttg gaggacgagt agtaaacaat gatcactttt tgtactgggg
961 tgacataata caaaatagtg aagatggagt agaatgcaaa attcatgtca ttgaacaaac
1021 agagttcatt gatgaccaga ctttcttgcc tcatcggagt acgaatttgc aaccatatat
1081 aaaacgtgca gctgcatcta aattgcagtc agcagaaaaa ctaatgtaca tttgcactga
1141 tcagctaggc ttagaacaag actttgaaca gaagcaaatg cctgaaggga agctcaacgt
1201 agatggattt ttattatgca ttgatgtaag tcaaggatgc aataggaagt ttgatgatca
1261 acttaaattt gtgaataacc tttttgtcca gttatcaaaa tcaaaaaaac ctgtaataat
1321 agcagcaact aaatgtgatg aatgcgtgga tcattatctt agagaagttc aggcatttgc
1381 ttcaaataaa aagaaccttc ttgtagtgga aacatcagca cgatttaatg tcaacattga
1441 aacatgtttt actgcactgg tacaaatgtt ggataaaact cgtagcaagc ctaaaattat
1501 tccctatttg gatgcttata aaacacagag acaacttgtt gtcacagcaa cagataagtt
1561 tgaaaaactt gtgcagactg tgagagatta tcatgcaact tggaaaactg ttagtaataa
1621 attaaaaaat catcctgatt atgaagaata catcaactta gagggaacaa gaaaggccag
1681 aaatacattc tcaaaacata tagaacaact taaacaggaa catataagaa aaaggagaga
1741 agagtatata aatactttac caagagcttt taacactctt ttgccaaatc tagaagagat
1801 tgaacatttg aattggtcag aagctttgaa gttaatggaa aagagagcag atttccagtt
1861 atgttttgtg gtgctagaaa aaactccttg ggatgaaact gaccatatag acaaaattaa
1921 tgataggcgg attccatttg acctcctgag cactttagaa gctgaaaaag tctatcagaa
1981 ccatgtacag catctgatat ccgagaagag gagggtggaa atgaaggaaa aattcaaaaa
2041 gactttggaa aaaattcaat tcatttcacc agggcagcca tgggaggaag ttatgtgctt
2101 tgttatggag gatgaagcct acaaatatat cactgaggct gatagcaaag aggtatatgg
2161 taggcatcag cgagaaatag ttgaaaaagc caaagaagag tttcaagaaa tgctttttga
2221 gcattctgaa cttttttatg atttagatct taatgcaaca cctagttcag ataaaatgag
2281 tgaaattcat acagttctga gtgaagaacc tagatataaa gctttacaga aacttgcacc
2341 tgatagggaa tcccttctac ttaagcatat aggatttgtt tatcatccca ctaaagaaac
2401 atgtcttagt ggccaaaatt gtacagacat taaagtggag cagttacttg ctagtagtct
2461 tttacagttg gatcatggcc gcttaagatt atatcacgat agtaccaata tagataaagt
2521 taaccttttt attttaggga aggatggcct tgcccaagaa ctagcaaatg agataaggac
2581 acaatccact gatgatgagt atgccttaga tggaaaaatt tatgaacttg atcttcggcc
2641 ggttgatgcc aaatcgcctt actttttgag tcagttatgg actgccgcct ttaaaccaca
2701 tgggtgcttc tgtgtattta attccattga gtcattgagt tttattgggg aatttattgg
2761 gaaaataaga actgaagctt ctcagatcag aaaagataaa tacatggcta atcttccatt
2821 tacattaatt ctggctaatc agagagattc cattagtaag aatctaccaa ttctcaggca
2881 ccaagggcag cagttggcaa acaagttgca atgtcctttt gtagatgtac ctgctggtac
2941 atatcctcgt aaatttaatg aaacccaaat aaagcaagct ctcagaggag tattggaatc
3001 agttaaacac aatttggatg tggtgagccc aattcctgcc aataaggact tatcagaagc
3061 tgacttgaga attgtcatgt gcgccatgtg tggagatcca tttagtgtgg atcttattct
3121 ttcacccttc cttgattctc attcttgcag tgctgctcaa gctggacaga ataattccct
3181 aatgcttgat aaaatcattg gtgaaaaaag gaggcgaata cagatcacaa tattatcata
3241 ccactcttca attggagtaa gaaaagatga actagttcat gggtatatat tagtttactc
3301 tgcaaaacgg aaagcttcga tgggaatgct tcgagcattt ctatcagaag ttcaagacac
3361 cattcctgta cagctggtgg cagttactga cagccaagca gatttttttg aaaatgaggc
3421 tatcaaagag ttaatgactg aaggagaaca cattgcaact gagatcactg ctaaatttac
3481 agcactgtat tctttatctc agtatcatcg gcaaactgag gtctttactc tgttttttag
3541 tgatgttcta gagaaaaaaa atatgataga aaattcttat ttgtctgata atacaaggga
3601 atcaacccat caaagtgaag atgtttttct accatctccc agagactgtt ttccctataa
3661 taactaccct gattcagatg atgacacaga agcaccacct ccttatagtc caattgggga
3721 tgatgtacag ttgcttccaa cacctagtga ccgttccaga tatagattag atttggaagg
3781 aaatgagtat cctattcata gtaccccaaa ctgtcatgac catgaacgca accataaagt
3841 gcctccacct attaaaccta aaccagttgt acctaagaca aatgtgaaaa aactcgatcc
3901 aaacctttta aaaacaattg aagctggtat tggtaaaaat ccaagaaagc agacttcccg
3961 ggtgcctttg gcacatcctg aagatatgga tccttcagat aactatgcgg aacccattga
4021 tacaattttc aaacagaagg gctattctga tgagatttat gttgtcccag atgatagtca
4081 aaatcgtatt aaaattcgaa actcatttgt aaataacacc caaggagatg aagaaaatgg
4141 gttttctgat agaacctcaa aaagtcatgg ggaacggagg ccttcaaaat acaaatataa
4201 atctaaaacc ttgtttagta aagccaagtc atactataga agaacacatt cagatgccag
4261 tgatgatgag gctttcacca cttctaaaac aaaaagaaaa ggaagacatc gtggaagtga
4321 agaagatcca cttctttctc ctgttgaaac ttggaaaggt aagtatcaag gttacaagac
4381 aggtttaagg agaccaataa gctattattc gtataggata ctttatacga agttattaag
4441 ttatggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag
4501 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
4561 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
4621 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
4681 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
4741 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
4801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
4861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
4921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
4981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
5041 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
5101 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
5161 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
5221 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
5281 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
5341 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
5401 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
5461 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
5521 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
5581 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
5641 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
5701 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
5761 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
5821 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
5881 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
5941 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
6001 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
6061 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
6121 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
6181 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
6241 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
6301 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
6361 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
6421 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
6481 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
6541 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
6601 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
6661 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
6721 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
6781 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
6841 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
6901 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
6961 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
7021 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
7081 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
7141 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
7201 ggccttttgc tcacatgt
//
