| 质粒名称: | pAAVLINK-EF1a-5'-PXDN |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001476 |
| 产品用途: | 5' AAVLINK plasmid for PXDN expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PXDN |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-PXDN
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-PXDN载体质粒基本信息
pAAVLINK-EF1a-5'-PXDN质粒图谱载体图谱和pAAVLINK-EF1a-5'-PXDN载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-PXDN质粒载体简介
pAAVLINK-EF1a-5'-PXDN质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-PXDN 6374 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6374)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6374
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
regulatory 695..704
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 701..3514
/codon_start=1
/label=PXDN
/translation="MAKRSRGPGRRCLLALVLFCAWGTLAVVAQKPGAGCPSRCLCFRT
TVRCMHLLLEAVPAVAPQTSILDLRFNRIREIQPGAFRRLRNLNTLLLNNNQIKRIPSG
AFEDLENLKYLYLYKNEIQSIDRQAFKGLASLEQLYLHFNQIETLDPDSFQHLPKLERL
FLHNNRITHLVPGTFNHLESMKRLRLDSNTLHCDCEILWLADLLKTYAESGNAQAAAIC
EYPRRIQGRSVATITPEELNCERPRITSEPQDADVTSGNTVYFTCRAEGNPKPEIIWLR
NNNELSMKTDSRLNLLDDGTLMIQNTQETDQGIYQCMAKNVAGEVKTQEVTLRYFGSPA
RPTFVIQPQNTEVLVGESVTLECSATGHPPPRISWTRGDRTPLPVDPRVNITPSGGLYI
QNVVQGDSGEYACSATNNIDSVHATAFIIVQALPQFTVTPQDRVVIEGQTVDFQCEAKG
NPPPVIAWTKGGSQLSVDRRHLVLSSGTLRISGVALHDQGQYECQAVNIIGSQKVVAHL
TVQPRVTPVFASIPSDTTVEVGANVQLPCSSQGEPEPAITWNKDGVQVTESGKFHISPE
GFLTINDVGPADAGRYECVARNTIGSASVSMVLSVNVPDVSRNGDPFVATSIVEAIATV
DRAINSTRTHLFDSRPRSPNDLLALFRYPRDPYTVEQARAGEIFERTLQLIQEHVQHGL
MVDLNGTSYHYNDLVSPQYLNLIANLSGCTAHRRVNNCSDMCFHQKYRTHDGTCNNLQH
PMWGASLTAFERLLKSVYENGFNTPRGINPHRLYNGHALPMPRLVSTTLIGTETVTPDE
QFTHMLMQWGQFLDHDLDSTVVALSQARFSDGQHCSNVCSNDPPCFSVMIPPNDSRARS
GARCMFFVRSSPVCGSGMTSLLMNSVYPREQINQLTSYIDASNVYGSTEHEARSIRDLA
SHRGLLRQ"
misc_feature 3420..3425
/label=SDS
intron 3515..3555
/label=Splicing Donor
misc_feature 3559..3592
/label=lox2272-JT1571
repeat_region 3637..3777
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3637..3766
/label=AAV2 ITR
rep_origin 3852..4307
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4589..4693
/gene="bla"
/label=AmpR promoter
CDS 4694..5554
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5725..6313
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atggccaagc gctccagggg
721 ccccgggcgc cgctgcctgt tggcgctcgt gctgttctgc gcctggggga cgctggccgt
781 ggtggcccag aagccgggcg cagggtgtcc gagccgctgc ctgtgcttcc gcaccaccgt
841 gcgctgcatg catctgctgc tggaggccgt gcccgccgtg gcgccgcaga cctccatcct
901 agatcttcgc tttaacagaa tcagagagat ccaacctggg gcattcaggc ggctgaggaa
961 cttgaacaca ttgcttctca ataataatca gatcaagagg atacctagtg gagcatttga
1021 agacttggaa aatttaaaat atctctatct gtacaagaat gagatccagt caattgacag
1081 gcaagcattt aagggacttg cctctctaga gcaactatac ctgcacttta atcagataga
1141 aactttggac ccagattcgt tccagcatct cccgaagctc gagaggctat ttttgcataa
1201 caaccggatt acacatttag ttccagggac atttaatcac ttggaatcta tgaagagatt
1261 gcgactggac tcaaacacac ttcactgcga ctgtgaaatc ctgtggttgg cggatttgct
1321 gaaaacctac gcggagtcgg ggaacgcgca ggcagcggcc atctgtgaat atcccagacg
1381 catccaggga cgctcagtgg caaccatcac cccggaagag ctgaactgtg aaaggccccg
1441 gatcacctcc gagccccagg acgcagatgt gacctcgggg aacaccgtgt acttcacctg
1501 cagagccgaa ggcaacccca agcctgagat catctggctg cgaaacaata atgagctgag
1561 catgaagaca gattcccgcc taaacttgct ggacgatggg accctgatga tccagaacac
1621 acaggagaca gaccagggta tctaccagtg catggcaaag aacgtggccg gagaggtgaa
1681 gacgcaagag gtgaccctca ggtacttcgg gtctccagct cgacccactt ttgtaatcca
1741 gccacagaat acagaggtgc tggttgggga gagcgtcacg ctggagtgca gcgccacagg
1801 ccaccccccg ccgcggatct cctggacgag aggtgaccgc acacccttgc cagttgaccc
1861 gcgggtgaac atcacgcctt ctggcgggct ttacatacag aacgtcgtac agggggacag
1921 cggagagtat gcgtgctctg cgaccaacaa cattgacagc gtccatgcca ccgctttcat
1981 catcgtccag gctcttcctc agttcactgt gacgcctcag gacagagtcg ttattgaggg
2041 ccagaccgtg gatttccagt gtgaagccaa gggcaacccg ccgcccgtca tcgcctggac
2101 caagggaggg agccagctct ccgtggaccg gcggcacctg gtcctgtcat cgggaacact
2161 tagaatctct ggtgttgccc tccacgacca gggccagtac gaatgccagg ctgtcaacat
2221 catcggctcc cagaaggtcg tggcccacct gactgtgcag cccagagtca ccccagtgtt
2281 tgccagcatt cccagcgaca caacagtgga ggtgggcgcc aatgtgcagc tcccgtgcag
2341 ctcccagggc gagcccgagc cagccatcac ctggaacaag gatggggttc aggtgacaga
2401 aagtggaaaa tttcacatca gccctgaagg attcttgacc atcaatgacg ttggccctgc
2461 agacgcaggt cgctatgagt gtgtggcccg gaacaccatt gggtcggcct cggtgagcat
2521 ggtgctcagt gtgaacgttc ctgacgtcag tcgaaatgga gatccgtttg tagctacctc
2581 catcgtggaa gcgattgcga ctgttgacag agctataaac tcaacccgaa cacatttgtt
2641 tgacagccgt cctcgttctc caaatgattt gctggccttg ttccggtatc cgagggatcc
2701 ttacacagtt gaacaggcac gggcgggaga aatctttgaa cggacattgc agctcattca
2761 ggagcatgta cagcatggct tgatggtcga cctcaacgga acaagttacc actacaacga
2821 cctggtgtct ccacagtacc tgaacctcat cgcaaacctg tcgggctgta ccgcccaccg
2881 gcgcgtgaac aactgctcgg acatgtgctt ccaccagaag taccggacgc acgacggcac
2941 ctgtaacaac ctgcagcacc ccatgtgggg cgcctcgctg accgccttcg agcgcctgct
3001 gaaatccgtg tacgagaatg gcttcaacac ccctcggggc atcaaccccc accgactgta
3061 caacgggcac gcccttccca tgccgcgcct ggtgtccacc accctgatcg ggacggagac
3121 cgtcacaccc gacgagcagt tcacccacat gctgatgcag tggggccagt tcctggacca
3181 cgacctcgac tccacggtgg tggccctgag ccaggcacgc ttctccgacg gacagcactg
3241 cagcaacgtg tgcagcaacg accccccctg cttctctgtc atgatccccc ccaatgactc
3301 ccgggccagg agcggggccc gctgcatgtt cttcgtgcgc tccagccctg tgtgcggcag
3361 cggcatgact tcgctgctca tgaactccgt gtacccgcgg gagcagatca accagctcac
3421 ctcctacatc gacgcatcca acgtgtacgg gagcacggag catgaggccc gcagcatccg
3481 cgacctggcc agccaccgcg gcctgctgcg gcaggtaagt atcaaggtta caagacaggt
3541 ttaaggagac caataagcta ttattcgtat aggatacttt atacgaagtt attaagttat
3601 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
3661 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
3721 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
3781 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
3841 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
3901 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
3961 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
4021 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
4081 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4141 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
4201 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
4261 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
4321 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
4381 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
4441 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
4501 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
4561 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
4621 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
4681 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
4741 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
4801 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
4861 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
4921 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
4981 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
5041 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
5101 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
5161 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
5221 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
5281 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5341 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5401 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
5461 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
5521 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
5581 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
5641 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
5701 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
5761 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
5821 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
5881 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
5941 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
6001 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
6061 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
6121 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
6181 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
6241 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6301 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6361 ttttgctcac atgt
//
