| 质粒名称: | pAAVLINK-EF1a-5'-ASTN2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001470 |
| 产品用途: | 5' AAVLINK plasmid for ASTN2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ASTN2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-ASTN2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-ASTN2载体质粒基本信息
pAAVLINK-EF1a-5'-ASTN2质粒图谱载体图谱和pAAVLINK-EF1a-5'-ASTN2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-ASTN2质粒载体简介
pAAVLINK-EF1a-5'-ASTN2质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-ASTN2 5295 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5295)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5295
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
regulatory 707..716
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 713..2435
/codon_start=1
/label=ASTN2
/translation="MAAAGARLSPGPGSGLRGRPRLCFHPGPPPLLPLLLLFLLLLPPP
PLLAGATAAASREPDSPCRLKTVTVSTLPALRESDIGWSGARAGAGAGTGAGAAAAAAS
PGSPGSAGTAAESRLLLFVRNELPGRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQ
QWLENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEEQMHILHISVMGGLIALLLLLLV
FTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARESFRSSRLQTHNSVIG
VPIRETPILDDYDCEEDEEPPRRANHVSREDEFGSQVTHTLDSLGHPGEEKVDFEKKAA
AEATQETVESLMQKFKESFRANTPIEIGQLQPPLRSTSAGKRKRRSKSRGGISFGRAKG
TSGSEADDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGSQMSCP
LTVKVTLHVPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQR
TTDACEQLCDPETGECSCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTLERGYDLVT"
misc_feature 1066..1071
/label=SDS
misc_feature 1599..1604
/label=SDS
misc_feature 1879..1884
/label=SDS
intron 2436..2476
/label=Splicing Donor
misc_feature 2480..2513
/label=lox2272-JT1571
repeat_region 2558..2698
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2558..2687
/label=AAV2 ITR
rep_origin 2773..3228
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3510..3614
/gene="bla"
/label=AmpR promoter
CDS 3615..4475
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4646..5234
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatggccgc
721 cgccggcgcc cggctcagcc ccggccccgg ctcggggctc cgggggcggc cgaggctctg
781 cttccacccg gggccgccgc cactgctgcc gctgctgctg ctgttcctgc tcctgctgcc
841 gccgccgccg ctgctggccg gcgccaccgc cgctgcctcg cgggagcccg acagcccgtg
901 ccggctgaag accgtcacgg tgtccacact gcccgccctg cgggagagcg acatcggctg
961 gagcggcgcc cgcgccgggg ccggggctgg gaccggggcc ggagccgccg ccgccgccgc
1021 gtccccgggc tctcctggct ctgccggcac cgccgccgag tcgcgcctcc tgctctttgt
1081 gcgtaacgag ctgccggggc gcatcgcggt gcaggacgac ctggacaaca ccgagctgcc
1141 cttcttcacc ctggagatgt ctggcacagc ggcggacatc tcgctggtgc actggagaca
1201 gcagtggctg gagaatggca ccttgtactt ccacgtctcc atgagcagct ccgggcagct
1261 ggcccaagcc accgccccca ctctccagga gccctcggag attgttgagg agcagatgca
1321 catcctccac atttctgtga tgggtggcct catcgcgctg ctgctgctgc tgctggtgtt
1381 caccgtggcg ctgtacgccc agcgacgttg gcagaagcgt cgccgcatcc cccagaagag
1441 cgcaagcaca gaagccactc atgagatcca ctacatccca tctgtgctgc tgggtcccca
1501 ggcgcgggag agcttccgtt catcccggct gcaaacccac aattccgtca ttggcgtgcc
1561 catccgggag actcccatcc tggatgacta tgactgtgag gaggatgagg agccacctag
1621 gcgggccaac catgtctccc gcgaggacga gtttggcagc caggtgaccc acactctgga
1681 cagtctggga catccagggg aagagaaggt ggactttgag aagaaagcag cagctgaggc
1741 gactcaggag acagtggagt ccctgatgca gaagttcaag gagagtttcc gcgctaacac
1801 gcccatcgag atcggtcagc tgcaaccacc cctgcgcagc acatcggcag ggaagaggaa
1861 gcggaggagc aagtctcgag gaggaatcag ctttgggaga gccaagggga cgtcgggctc
1921 agaggcagac gatgaaactc agctgacatt ctacacggag cagtaccgca gtcgccgccg
1981 cagcaaaggt ttgctgaaaa gcccagtgaa caagacagcc ctgacactga ttgctgtgag
2041 ttcctgcatc ctggccatgg tgtgtggcag ccagatgtct tgtccactca ctgtgaaggt
2101 gactctgcat gtgcccgagc acttcatagc agatggaagc agcttcgtgg tgagtgaagg
2161 gagctacctg gacatctccg actggttaaa cccagccaag ctttccctgt attaccagat
2221 caatgccacc tccccatggg tgagggacct ctgtggacaa aggacgacag atgcctgtga
2281 gcagctctgc gacccagaaa ccggagagtg cagctgtcat gaaggctatg cccctgaccc
2341 tgttcacaga cacctgtgtg tgcgcagtga ctggggacag agtgaaggac cttggcccta
2401 cacgacactt gagaggggct atgatctggt gacaggtaag tatcaaggtt acaagacagg
2461 tttaaggaga ccaataagct attattcgta taggatactt tatacgaagt tattaagtta
2521 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
2581 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
2641 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
2701 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
2761 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
2821 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
2881 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
2941 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
3001 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
3061 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
3121 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
3181 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
3241 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
3301 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
3361 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
3421 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
3481 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
3541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
3601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
3661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
3721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
3781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
3841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
3901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
3961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
4021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
4081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
4141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
4201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
4261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
4321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
4381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
4441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
4501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
4561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
4621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
4681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
4741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
4801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
4861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
4921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
4981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
5041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
5101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
5161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
5221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
5281 cttttgctca catgt
//
