| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pAAV-CAG-RFP |
| 质粒类型: | 腺病毒载体；荧光蛋白报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CAG (CMV enhancer/chicken β-actin promoter) |
| 载体大小: | 7218 bp |
| 5' 测序引物及序列: | pCAG fwd primer 5’CAACGTGCTGGTTATTGTG 3’ |
| 3' 测序引物及序列: | SP6 fwd primer 5’ATTTAGGTGACACTATAG 3’ |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | DsRedExpress |
| 克隆菌株: | Stbl3 或 NEB Stble |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: | -- |
| 产品目录号: | #22910 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pAAV-CAG-RFP载体 This AAV is a RFP construct driven by the ubiquitous CAG promoter. The roughly 1.8Kb CAG promoter (also known as CBA promoter or CAGGS promoter) is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian cells. The CAG promoter is composed of the following regulatory elements: (C) cytomegalovirus (CMV) early enhancer element; (A) the promoter region, the first exon, and the first intron of chicken beta-Actin gene, and (G) the splice acceptor of the rabbit beta-Globin gene. Like the EF1a promoter, the CAG promoter is commonly used as an alternative for the CMV promoter, from which the expression is decreased due to methylation/silencing. In many cell types tested, the CAG and EF1a promoters give much higher levels of expression than other commonly used cellular promoters such as the UBC and PGK promoters. RFP has an Excitation/Emission spectrum of 500nm/580nm. Other fluorescent proteins with similar spectrums include Kusabira Orange , mOrange, mOrange2, dTomato, tdTomato, DsRed, DsRed2, TurboRFP, and TagRFP
载体序列
LOCUS pAAV-CAG-RFP Sequencing Result 7218 bp DNA SYN
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-CAG-RFP|
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..7218
/organism="pAAV-CAG-RFP Sequencing Result"
/mol_type="other DNA"
CDS complement(108..716)
/label="ORF frame 2"
/translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
misc_feature 1051..1070
/label="pCAG_F_primer"
/translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
misc_feature 1291..1674
/label="bGlob_int"
/translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
CDS 1600..2454
/label="ORF frame 1"
/translation="MFIPLIFLPQLLGNVLVCVLAHHFGKAREFEDLAASASRTSGSP
GLQEFDATGRHHGLLRGRHQGVHALQGAHGGLRERPRVRDRGRGRGPPLRGHPDRQAE
GDQGRPPALRLGHPVPPVPVRLQGVREAPRRHPRLQEAVLPRGLQVGARDELRGRRRG
DRDPGLLPAGRLLHLQGEVHRRELPLRRPRNAEEDYGLGGLHRAPVPPRRRAEGRDPQ
GPEAEGRRPLPGGVQVHLHGQEARAAARLLLRGLQAGHHLPQRGLHHRGAVRARRGPP
PPVPVAAK*"
misc_feature 1711..1727
/label="pBluescriptSK_primer"
/translation="MFIPLIFLPQLLGNVLVCVLAHHFGKAREFEDLAASASRTSGSP
GLQEFDATGRHHGLLRGRHQGVHALQGAHGGLRERPRVRDRGRGRGPPLRGHPDRQAE
GDQGRPPALRLGHPVPPVPVRLQGVREAPRRHPRLQEAVLPRGLQVGARDELRGRRRG
DRDPGLLPAGRLLHLQGEVHRRELPLRRPRNAEEDYGLGGLHRAPVPPRRRAEGRDPQ
GPEAEGRRPLPGGVQVHLHGQEARAAARLLLRGLQAGHHLPQRGLHHRGAVRARRGPP
PPVPVAAK*"
CDS 1748..2443
/label="ORF frame 2"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
gene 1766..2443
/label="DsRed_Express_2"
/gene="DsRed_Express_2"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
gene 1769..2440
/label="DsRed_Express"
/gene="DsRed_Express"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
misc_feature complement(1946..1966)
/label="dsRed1_N_primer"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
misc_feature 2358..2381
/label="dsRed1_C_primer"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
misc_feature complement(2486..2503)
/label="Sp6_primer"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
promoter complement(2487..2504)
/label="Sp6_promoter"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
misc_feature complement(2523..2540)
/label="BGH_rev_primer"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
terminator 2526..2717
/label="bGH_PA_terminator"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
rep_origin 3301..3607
/label="f1_origin"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
misc_feature 4398..4420
/label="pGEX_3_primer"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
promoter 4579..4607
/label="AmpR_promoter"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
gene 4649..5509
/label="Ampicillin"
/gene="Ampicillin"
/translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
QYERAEGRHHLFL*"
CDS 4649..5509
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 5664..6283
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 6666..6953
/label="CAG_enhancer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC
61 GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA CTCCCACAGG
121 TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT TAATGACGGC
181 TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT CCGGGAGGGC CCTTTGTGCG
241 GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCT
301 CCGCGCTGCC CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC GGGGCTTTGT GCGCTCCGCA
361 GTGTGCGCGA GGGGAGCGCG GCCGGGGGCG GTGCCCCGCG GTGCGGGGGG GGCTGCGAGG
421 GGAACAAAGG CTGCGTGCGG GGTGTGTGCG TGGGGGGGTG AGCAGGGGGT GTGGGCGCGT
481 CGGTCGGGCT GCAACCCCCC CTGCACCCCC CTCCCCGAGT TGCTGAGCAC GGCCCGGCTT
541 CGGGTGCGGG GCTCCGTACG GGGCGTGGCG CGGGGCTCGC CGTGCCGGGC GGGGGGTGGC
601 GGCAGGTGGG GGTGCCGGGC GGGGCGGGGC CGCCTCGGGC CGGGGAGGGC TCGGGGGAGG
661 GGCGCGGCGG CCCCCGGAGC GCCGGCGGCT GTCGAGGCGC GGCGAGCCGC AGCCATTGCC
721 TTTTATGGTA ATCGTGCGAG AGGGCGCAGG GACTTCCTTT GTCCCAAATC TGTGCGGAGC
781 CGAAATCTGG GAGGCGCCGC CGCACCCCCT CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG
841 CCGGCAGGAA GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC
901 TCCCTCTCCA GCCTCGGGGC TGTCCGCGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA
961 GGGCGGGGTT CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC
1021 ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC
1081 ATTTTGGCAA AGAATTGTGG CGGCCCGACC GCGGGCTAGC GGCGCGCCGA GCTCGGATCC
1141 TGAGAACTTC AGGGTGAGTC TATGGGACCC TTGATGTTTT CTTTCCCCTT CTTTTCTATG
1201 GTTAAGTTCA TGTCATAGGA AGGGGAGAAG TAACAGGGTA CACATATTGA CCAAATCAGG
1261 GTAATTTTGC ATTTGTAATT TTAAAAAATG CTTTCTTCTT TTAATATACT TTTTTGTTTA
1321 TCTTATTTCT AATACTTTCC CTAATCTCTT TCTTTCAGGG CAATAATGAT ACAATGTATC
1381 ATGCCTCTTT GCACCATTCT AAAGAATAAC AGTGATAATT TCTGGGTTAA GGCAATAGCA
1441 ATATTTCTGC ATATAAATAT TTCTGCATAT AAATTGTAAC TGATGTAAGA GGTTTCATAT
1501 TGCTAATAGC AGCTACAATC CAGCTACCAT TCTGCTTTTA TTTTATGGTT GGGATAAGGC
1561 TGGATTATTC TGAGTCCAAG CTAGGCCCTT TTGCTAATCA TGTTCATACC TCTTATCTTC
1621 CTCCCACAGC TCCTGGGCAA CGTGCTGGTC TGTGTGCTGG CCCATCACTT TGGCAAAGCA
1681 CGTGAATTCG AAGATCTGGC CGCCTCGGCC TCTAGAACTA GTGGATCCCC CGGGCTGCAG
1741 GAATTCGATG CTACCGGTCG CCACCATGGC CTCCTCCGAG GACGTCATCA AGGAGTTCAT
1801 GCGCTTCAAG GTGCGCATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA
1861 GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGCGG
1921 CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCCCAGTTC CAGTACGGCT CCAAGGTGTA
1981 CGTGAAGCAC CCCGCCGACA TCCCCGACTA CAAGAAGCTG TCCTTCCCCG AGGGCTTCAA
2041 GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC
2101 CCTGCAGGAC GGCTCCTTCA TCTACAAGGT GAAGTTCATC GGCGTGAACT TCCCCTCCGA
2161 CGGCCCCGTA ATGCAGAAGA AGACTATGGG CTGGGAGGCC TCCACCGAGC GCCTGTACCC
2221 CCGCGACGGC GTGCTGAAGG GCGAGATCCA CAAGGCCCTG AAGCTGAAGG ACGGCGGCCA
2281 CTACCTGGTG GAGTTCAAGT CCATCTACAT GGCCAAGAAG CCCGTGCAGC TGCCCGGCTA
2341 CTACTACGTG GACTCCAAGC TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA
2401 GCAGTACGAG CGCGCCGAGG GCCGCCACCA CCTGTTCCTG TAGCGGCCAA ATGAATTCCC
2461 AAACCGTACG GGAAACGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG CTCGCTGATC
2521 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC
2581 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC
2641 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG
2701 GGAGGATTGG GAAGACAATA GCGCGGCCGC TCTAGAGCAT GGCTACGTAG ATAAGTAGCA
2761 TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT
2821 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC
2881 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGGCGT AATAGCGAAG AGGCCCGCAC
2941 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGATTCC GTTGCAATGG
3001 CTGGCGGTAA TATTGTTCTG GATATTACCA GCAAGGCCGA TAGTTTGAGT TCTTCTACTC
3061 AGGCAAGTGA TGTTATTACT AATCAAAGAA GTATTGCGAC AACGGTTAAT TTGCGTGATG
3121 GACAGACTCT TTTACTCGGT GGCCTCACTG ATTATAAAAA CACTTCTCAG GATTCTGGCG
3181 TACCGTTCCT GTCTAAAATC CCTTTAATCG GCCTCCTGTT TAGCTCCCGC TCTGATTCTA
3241 ACGAGGAAAG CACGTTATAC GTGCTCGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC
3301 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC
3361 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
3421 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC
3481 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG
3541 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT
3601 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT
3661 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
3721 ATATTAACGC TTACAATTTA AATATTTGCT TATACAATCT TCCTGTTTTT GGGGCTTTTC
3781 TGATTATCAA CCGGGGTACA TATGATTGAC ATGCTAGTTT TACGATTACC GTTCATCGAT
3841 TCTCTTGTTT GCTCCAGACT CTCAGGCAAT GACCTGATAG CCTTTGTAGA GACCTCTCAA
3901 AAATAGCTAC CCTCTCCGGC ATGAATTTAT CAGCTAGAAC GGTTGAATAT CATATTGATG
3961 GTGATTTGAC TGTCTCCGGC CTTTCTCACC CGTTTGAATC TTTACCTACA CATTACTCAG
4021 GCATTGCATT TAAAATATAT GAGGGTTCTA AAAATTTTTA TCCTTGCGTT GAAATAAAGG
4081 CTTCTCCCGC AAAAGTATTA CAGGGTCATA ATGTTTTTGG TACAACCGAT TTAGCTTTAT
4141 GCTCTGAGGC TTTATTGCTT AATTTTGCTA ATTCTTTGCC TTGCCTGTAT GATTTATTGG
4201 ATGTTGGAAT CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG
4261 CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA
4321 CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG
4381 ACAAGCTGTG ACCGTCTccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
4441 acgcgcgagA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT
4501 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
4561 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT
4621 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT
4681 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT
4741 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG
4801 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA
4861 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG
4921 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT
4981 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC
5041 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA
5101 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT
5161 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT
5221 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC
5281 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA
5341 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG
5401 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG
5461 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA
5521 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA
5581 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
5641 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
5701 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
5761 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
5821 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
5881 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
5941 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
6001 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
6061 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
6121 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
6181 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
6241 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
6301 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
6361 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
6421 CAGCGAGTCA GTGAGCGAGC GAGCGCGCAG AGAGGGAGTG GCCAACTCCA TCACTAGGGG
6481 TTCCTTGTAG TTAATGATTA ACCCGCCATG CTACTTATCT ACGTAGCCAT GCTCTAGAGG
6541 ATCCTTCGAA AAGCTTGTAT ACGGCCGGCC ACGCGTTCGC CACTCGACAT TGATTATTGA
6601 CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC
6661 GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT
6721 TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC
6781 AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC
6841 CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT
6901 ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA
6961 CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC CCCTCCCCAC
7021 CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG GCGGGGGGGG
7081 GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG GCGGGGCGGG GCGAGGCGGA
7141 GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA GTTTCCTTTT ATGGCGAGGC
7201 GGCGGCGGCG GCGGCCCT
//
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载体质粒应用举例
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