pAAV-CAG-RFP

价格:1100元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pAAV-CAG-RFP
质粒类型: 腺病毒载体;荧光蛋白报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CAG (CMV enhancer/chicken β-actin promoter)
载体大小: 7218 bp
5' 测序引物及序列: pCAG fwd primer 5’CAACGTGCTGGTTATTGTG 3’
3' 测序引物及序列: SP6 fwd primer 5’ATTTAGGTGACACTATAG 3’
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: DsRedExpress
克隆菌株: Stbl3 或 NEB Stble
宿主细胞(系): 常规哺乳动物细胞系
备注: --
产品目录号: #22910
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pAAV-CAG-RFP载体图谱



载体简介

pAAV-CAG-RFP载体

This AAV is a RFP construct driven by the ubiquitous CAG promoter.

The roughly 1.8Kb CAG promoter (also known as CBA promoter or CAGGS promoter) is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian cells. The CAG promoter is composed of the following regulatory elements: (C) cytomegalovirus (CMV) early enhancer element; (A) the promoter region, the first exon, and the first intron of chicken beta-Actin gene, and (G) the splice acceptor of the rabbit beta-Globin gene. Like the EF1a promoter, the CAG promoter is commonly used as an alternative for the CMV promoter, from which the expression is decreased due to methylation/silencing. In many cell types tested, the CAG and EF1a promoters give much higher levels of expression than other commonly used cellular promoters such as the UBC and PGK promoters.

RFP has an Excitation/Emission spectrum of 500nm/580nm. Other fluorescent proteins with similar spectrums include Kusabira Orange , mOrange, mOrange2, dTomato, tdTomato, DsRed, DsRed2, TurboRFP, and TagRFP

载体序列

LOCUS       pAAV-CAG-RFP Sequencing Result	7218 bp 	DNA	SYN
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pAAV-CAG-RFP|
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="pAAV-CAG-RFP Sequencing Result"
                     /mol_type="other DNA"
     CDS             complement(108..716)
                     /label="ORF frame 2"
                     /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
                     HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                     PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
                     QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
     misc_feature    1051..1070
                     /label="pCAG_F_primer"
                     /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
                     HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                     PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
                     QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
     misc_feature    1291..1674
                     /label="bGlob_int"
                     /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
                     HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                     PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
                     QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
     CDS             1600..2454
                     /label="ORF frame 1"
                     /translation="MFIPLIFLPQLLGNVLVCVLAHHFGKAREFEDLAASASRTSGSP
                     GLQEFDATGRHHGLLRGRHQGVHALQGAHGGLRERPRVRDRGRGRGPPLRGHPDRQAE
                     GDQGRPPALRLGHPVPPVPVRLQGVREAPRRHPRLQEAVLPRGLQVGARDELRGRRRG
                     DRDPGLLPAGRLLHLQGEVHRRELPLRRPRNAEEDYGLGGLHRAPVPPRRRAEGRDPQ
                     GPEAEGRRPLPGGVQVHLHGQEARAAARLLLRGLQAGHHLPQRGLHHRGAVRARRGPP
                     PPVPVAAK*"
     misc_feature    1711..1727
                     /label="pBluescriptSK_primer"
                     /translation="MFIPLIFLPQLLGNVLVCVLAHHFGKAREFEDLAASASRTSGSP
                     GLQEFDATGRHHGLLRGRHQGVHALQGAHGGLRERPRVRDRGRGRGPPLRGHPDRQAE
                     GDQGRPPALRLGHPVPPVPVRLQGVREAPRRHPRLQEAVLPRGLQVGARDELRGRRRG
                     DRDPGLLPAGRLLHLQGEVHRRELPLRRPRNAEEDYGLGGLHRAPVPPRRRAEGRDPQ
                     GPEAEGRRPLPGGVQVHLHGQEARAAARLLLRGLQAGHHLPQRGLHHRGAVRARRGPP
                     PPVPVAAK*"
     CDS             1748..2443
                     /label="ORF frame 2"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     gene            1766..2443
                     /label="DsRed_Express_2"
                     /gene="DsRed_Express_2"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     gene            1769..2440
                     /label="DsRed_Express"
                     /gene="DsRed_Express"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     misc_feature    complement(1946..1966)
                     /label="dsRed1_N_primer"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     misc_feature    2358..2381
                     /label="dsRed1_C_primer"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     misc_feature    complement(2486..2503)
                     /label="Sp6_primer"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     promoter        complement(2487..2504)
                     /label="Sp6_promoter"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     misc_feature    complement(2523..2540)
                     /label="BGH_rev_primer"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     terminator      2526..2717
                     /label="bGH_PA_terminator"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     rep_origin      3301..3607
                     /label="f1_origin"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     misc_feature    4398..4420
                     /label="pGEX_3_primer"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     promoter        4579..4607
                     /label="AmpR_promoter"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     gene            4649..5509
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     CDS             4649..5509
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5664..6283
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6666..6953
                     /label="CAG_enhancer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC
   61 GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA CTCCCACAGG
  121 TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT TAATGACGGC
  181 TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT CCGGGAGGGC CCTTTGTGCG
  241 GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCT
  301 CCGCGCTGCC CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC GGGGCTTTGT GCGCTCCGCA
  361 GTGTGCGCGA GGGGAGCGCG GCCGGGGGCG GTGCCCCGCG GTGCGGGGGG GGCTGCGAGG
  421 GGAACAAAGG CTGCGTGCGG GGTGTGTGCG TGGGGGGGTG AGCAGGGGGT GTGGGCGCGT
  481 CGGTCGGGCT GCAACCCCCC CTGCACCCCC CTCCCCGAGT TGCTGAGCAC GGCCCGGCTT
  541 CGGGTGCGGG GCTCCGTACG GGGCGTGGCG CGGGGCTCGC CGTGCCGGGC GGGGGGTGGC
  601 GGCAGGTGGG GGTGCCGGGC GGGGCGGGGC CGCCTCGGGC CGGGGAGGGC TCGGGGGAGG
  661 GGCGCGGCGG CCCCCGGAGC GCCGGCGGCT GTCGAGGCGC GGCGAGCCGC AGCCATTGCC
  721 TTTTATGGTA ATCGTGCGAG AGGGCGCAGG GACTTCCTTT GTCCCAAATC TGTGCGGAGC
  781 CGAAATCTGG GAGGCGCCGC CGCACCCCCT CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG
  841 CCGGCAGGAA GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC
  901 TCCCTCTCCA GCCTCGGGGC TGTCCGCGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA
  961 GGGCGGGGTT CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC
 1021 ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC
 1081 ATTTTGGCAA AGAATTGTGG CGGCCCGACC GCGGGCTAGC GGCGCGCCGA GCTCGGATCC
 1141 TGAGAACTTC AGGGTGAGTC TATGGGACCC TTGATGTTTT CTTTCCCCTT CTTTTCTATG
 1201 GTTAAGTTCA TGTCATAGGA AGGGGAGAAG TAACAGGGTA CACATATTGA CCAAATCAGG
 1261 GTAATTTTGC ATTTGTAATT TTAAAAAATG CTTTCTTCTT TTAATATACT TTTTTGTTTA
 1321 TCTTATTTCT AATACTTTCC CTAATCTCTT TCTTTCAGGG CAATAATGAT ACAATGTATC
 1381 ATGCCTCTTT GCACCATTCT AAAGAATAAC AGTGATAATT TCTGGGTTAA GGCAATAGCA
 1441 ATATTTCTGC ATATAAATAT TTCTGCATAT AAATTGTAAC TGATGTAAGA GGTTTCATAT
 1501 TGCTAATAGC AGCTACAATC CAGCTACCAT TCTGCTTTTA TTTTATGGTT GGGATAAGGC
 1561 TGGATTATTC TGAGTCCAAG CTAGGCCCTT TTGCTAATCA TGTTCATACC TCTTATCTTC
 1621 CTCCCACAGC TCCTGGGCAA CGTGCTGGTC TGTGTGCTGG CCCATCACTT TGGCAAAGCA
 1681 CGTGAATTCG AAGATCTGGC CGCCTCGGCC TCTAGAACTA GTGGATCCCC CGGGCTGCAG
 1741 GAATTCGATG CTACCGGTCG CCACCATGGC CTCCTCCGAG GACGTCATCA AGGAGTTCAT
 1801 GCGCTTCAAG GTGCGCATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA
 1861 GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGCGG
 1921 CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCCCAGTTC CAGTACGGCT CCAAGGTGTA
 1981 CGTGAAGCAC CCCGCCGACA TCCCCGACTA CAAGAAGCTG TCCTTCCCCG AGGGCTTCAA
 2041 GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC
 2101 CCTGCAGGAC GGCTCCTTCA TCTACAAGGT GAAGTTCATC GGCGTGAACT TCCCCTCCGA
 2161 CGGCCCCGTA ATGCAGAAGA AGACTATGGG CTGGGAGGCC TCCACCGAGC GCCTGTACCC
 2221 CCGCGACGGC GTGCTGAAGG GCGAGATCCA CAAGGCCCTG AAGCTGAAGG ACGGCGGCCA
 2281 CTACCTGGTG GAGTTCAAGT CCATCTACAT GGCCAAGAAG CCCGTGCAGC TGCCCGGCTA
 2341 CTACTACGTG GACTCCAAGC TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA
 2401 GCAGTACGAG CGCGCCGAGG GCCGCCACCA CCTGTTCCTG TAGCGGCCAA ATGAATTCCC
 2461 AAACCGTACG GGAAACGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG CTCGCTGATC
 2521 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC
 2581 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC
 2641 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG
 2701 GGAGGATTGG GAAGACAATA GCGCGGCCGC TCTAGAGCAT GGCTACGTAG ATAAGTAGCA
 2761 TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT
 2821 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC
 2881 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGGCGT AATAGCGAAG AGGCCCGCAC
 2941 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGATTCC GTTGCAATGG
 3001 CTGGCGGTAA TATTGTTCTG GATATTACCA GCAAGGCCGA TAGTTTGAGT TCTTCTACTC
 3061 AGGCAAGTGA TGTTATTACT AATCAAAGAA GTATTGCGAC AACGGTTAAT TTGCGTGATG
 3121 GACAGACTCT TTTACTCGGT GGCCTCACTG ATTATAAAAA CACTTCTCAG GATTCTGGCG
 3181 TACCGTTCCT GTCTAAAATC CCTTTAATCG GCCTCCTGTT TAGCTCCCGC TCTGATTCTA
 3241 ACGAGGAAAG CACGTTATAC GTGCTCGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC
 3301 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC
 3361 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
 3421 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC
 3481 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG
 3541 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT
 3601 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT
 3661 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
 3721 ATATTAACGC TTACAATTTA AATATTTGCT TATACAATCT TCCTGTTTTT GGGGCTTTTC
 3781 TGATTATCAA CCGGGGTACA TATGATTGAC ATGCTAGTTT TACGATTACC GTTCATCGAT
 3841 TCTCTTGTTT GCTCCAGACT CTCAGGCAAT GACCTGATAG CCTTTGTAGA GACCTCTCAA
 3901 AAATAGCTAC CCTCTCCGGC ATGAATTTAT CAGCTAGAAC GGTTGAATAT CATATTGATG
 3961 GTGATTTGAC TGTCTCCGGC CTTTCTCACC CGTTTGAATC TTTACCTACA CATTACTCAG
 4021 GCATTGCATT TAAAATATAT GAGGGTTCTA AAAATTTTTA TCCTTGCGTT GAAATAAAGG
 4081 CTTCTCCCGC AAAAGTATTA CAGGGTCATA ATGTTTTTGG TACAACCGAT TTAGCTTTAT
 4141 GCTCTGAGGC TTTATTGCTT AATTTTGCTA ATTCTTTGCC TTGCCTGTAT GATTTATTGG
 4201 ATGTTGGAAT CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG
 4261 CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA
 4321 CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG
 4381 ACAAGCTGTG ACCGTCTccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
 4441 acgcgcgagA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT
 4501 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
 4561 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT
 4621 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT
 4681 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT
 4741 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG
 4801 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA
 4861 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG
 4921 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT
 4981 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC
 5041 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA
 5101 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT
 5161 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT
 5221 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC
 5281 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA
 5341 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG
 5401 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG
 5461 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA
 5521 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA
 5581 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
 5641 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
 5701 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
 5761 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
 5821 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
 5881 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
 5941 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
 6001 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
 6061 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
 6121 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
 6181 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
 6241 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
 6301 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
 6361 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
 6421 CAGCGAGTCA GTGAGCGAGC GAGCGCGCAG AGAGGGAGTG GCCAACTCCA TCACTAGGGG
 6481 TTCCTTGTAG TTAATGATTA ACCCGCCATG CTACTTATCT ACGTAGCCAT GCTCTAGAGG
 6541 ATCCTTCGAA AAGCTTGTAT ACGGCCGGCC ACGCGTTCGC CACTCGACAT TGATTATTGA
 6601 CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC
 6661 GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT
 6721 TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC
 6781 AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC
 6841 CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT
 6901 ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA
 6961 CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC CCCTCCCCAC
 7021 CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG GCGGGGGGGG
 7081 GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG GCGGGGCGGG GCGAGGCGGA
 7141 GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA GTTTCCTTTT ATGGCGAGGC
 7201 GGCGGCGGCG GCGGCCCT
//

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