pLV-mCherry

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联系方式:I47-825O-882O

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载体基本信息

质粒名称: pLV-mCherry 
出品公司: Addgene
目录编号: 36084
存储实验室: Pantelis Tsoulfas
用途: 第三代慢病毒载体,表达mCherry荧光蛋白

载体骨架基本信息
载体名称: pLenti-MP2
载体抗性: 氨苄霉素
空载体大小: 6782 bp
质粒大小: 7413 bp
载体类型: 哺乳细胞表达,慢病毒
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: mCherry
物种来源: 合成
插入大小: 704 bp
克隆方法: 限制性内切酶
5' 克隆位点: BamHI(未破坏)
3' 克隆位点: SalI(未破坏)
5' 测序引物 CMV-F
3' 测序引物 WPRE-R

文献引用方法
材料和方法部分: pLV-mCherry was a gift from Pantelis Tsoulfas (Addgene plasmid # 36084)

载体质粒图谱和多克隆位点信息

pLV-mCherry载体图谱

载体简介

载体序列

LOCUS       pLV-mCherry	7413 bp 	DNA	SYN
DEFINITION  pLV-mCherry
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="pLV-mCherry"
                     /mol_type="other DNA"
     CDS             15..725
                     /label="ORF frame 3"
     gene            18..722
                     /label="mCherry"
                     /gene="mCherry"
     misc_feature    195..248
                     /label="Q66M (mCherry)"
     misc_feature    734..1309
                     /label="WPRE"
     CDS             822..1346
                     /label="ORF frame 3"
     misc_feature    1390..1411
                     /label="U3PPT"
     misc_feature    1390..1405
                     /label="cPPT"
     misc_feature    1407..1459
                     /label="delta_U3"
     misc_feature    1460..1640
                     /label="HIV-1_5_LTR"
     misc_feature    1460..1640
                     /label="truncHIV-1_3_LTR"
     terminator      1715..1834
                     /label="SV40_PA_terminator"
     misc_feature    1803..1822
                     /label="EBV_rev_primer"
     rep_origin      1852..1950
                     /label="SV40_origin"
     misc_feature    1935..1954
                     /label="SV40pro_F_primer"
     promoter        complement(2029..2047)
                     /label="T7_promoter"
     promoter        complement(2057..2073)
                     /label="M13_forward20_primer"
     misc_feature    complement(2066..2088)
                     /label="M13_pUC_fwd_primer"
     misc_feature    2066..2214
                     /label="lacZ_a"
     rep_origin      2232..2538
                     /label="f1_origin"
     promoter        2731..2759
                     /label="AmpR_promoter"
     gene            2801..3661
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             2801..3661
                     /label="ORF frame 2"
     rep_origin      3816..4435
                     /label="pBR322_origin"
     promoter        4744..4773
                     /label="lac_promoter"
     misc_feature    4787..4809
                     /label="M13_pUC_rev_primer"
     promoter        4808..4826
                     /label="M13_reverse_primer"
     promoter        4843..4862
                     /label="T3_promoter"
     misc_feature    5116..5296
                     /label="HIV-1_5_LTR"
     misc_feature    5116..5296
                     /label="truncHIV-1_3_LTR"
     misc_feature    5407..5451
                     /label="HIV-1_psi_pack"
     CDS             5839..7026
                     /label="ORF frame 1"
     misc_feature    5961..6194
                     /label="RRE"
     misc_feature    6694..6709
                     /label="cPPT"
     promoter        6826..7330
                     /label="CMV_immearly_promoter"
     misc_feature    6833..7120
                     /label="CAG_enhancer"
     misc_feature    7287..7307
                     /label="CMV_fwd_primer"
     promoter        7288..7357
                     /label="CMV_promoter"
     misc_feature    7331..7350
                     /label="pCEP_fwd_primer"
     misc_feature    7333..7357
                     /label="LNCX_primer"
ORIGIN
    1 gatccgccgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
   61 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
  121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
  181 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
  241 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
  301 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
  361 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
  421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
  481 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
  541 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
  601 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
  661 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
  721 agtaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
  781 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
  841 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
  901 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
  961 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
 1021 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
 1081 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
 1141 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
 1201 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
 1261 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
 1321 ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
 1381 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
 1441 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
 1501 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
 1561 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
 1621 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
 1681 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
 1741 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
 1801 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
 1861 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
 1921 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
 1981 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
 2041 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
 2101 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
 2161 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
 2221 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
 2281 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
 2341 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
 2401 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
 2461 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
 2521 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
 2581 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
 2641 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
 2701 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
 2761 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
 2821 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
 2881 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
 2941 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
 3001 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
 3061 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
 3121 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
 3181 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
 3241 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
 3301 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
 3361 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
 3421 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
 3481 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
 3541 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
 3601 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
 3661 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
 3721 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
 3781 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
 3841 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
 3901 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
 3961 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
 4021 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
 4081 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
 4141 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
 4201 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
 4261 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
 4321 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
 4381 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
 4441 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
 4501 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
 4561 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
 4621 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
 4681 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
 4741 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
 4801 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
 4861 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
 4921 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
 4981 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
 5041 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
 5101 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
 5161 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
 5221 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
 5281 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
 5341 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
 5401 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
 5461 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
 5521 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
 5581 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
 5641 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
 5701 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
 5761 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
 5821 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
 5881 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
 5941 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
 6001 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
 6061 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
 6121 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
 6181 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
 6241 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
 6301 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
 6361 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
 6421 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
 6481 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
 6541 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
 6601 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
 6661 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
 6721 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
 6781 aaacaaatta caaaattcaa aattttatcg ataagcttgg gagttccgcg ttacataact
 6841 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
 6901 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
 6961 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
 7021 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
 7081 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
 7141 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
 7201 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
 7261 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
 7321 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
 7381 ttttgacctc catagaagac accgactcta gag
//

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