| 质粒名称: | pLV-mCherry |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 36084 |
| 存储实验室: | Pantelis Tsoulfas |
| 用途: | 第三代慢病毒载体，表达mCherry荧光蛋白 |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti-MP2 |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 6782 bp |
| 质粒大小: | 7413 bp |
| 载体类型: | 哺乳细胞表达,慢病毒 |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mCherry |
| 物种来源: | 合成 |
| 插入大小: | 704 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | SalI(未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | pLV-mCherry was a gift from Pantelis Tsoulfas (Addgene plasmid # 36084) |
买家导航
pLV-mCherry载体质粒基本信息
pLV-mCherry质粒图谱载体图谱和pLV-mCherry载体序列质粒序列多克隆位点信息
pLV-mCherry质粒载体简介
pLV-mCherry质粒序列载体序列
LOCUS pLV-mCherry 7413 bp DNA SYN
DEFINITION pLV-mCherry
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..7413
/organism="pLV-mCherry"
/mol_type="other DNA"
CDS 15..725
/label="ORF frame 3"
gene 18..722
/label="mCherry"
/gene="mCherry"
misc_feature 195..248
/label="Q66M (mCherry)"
misc_feature 734..1309
/label="WPRE"
CDS 822..1346
/label="ORF frame 3"
misc_feature 1390..1411
/label="U3PPT"
misc_feature 1390..1405
/label="cPPT"
misc_feature 1407..1459
/label="delta_U3"
misc_feature 1460..1640
/label="HIV-1_5_LTR"
misc_feature 1460..1640
/label="truncHIV-1_3_LTR"
terminator 1715..1834
/label="SV40_PA_terminator"
misc_feature 1803..1822
/label="EBV_rev_primer"
rep_origin 1852..1950
/label="SV40_origin"
misc_feature 1935..1954
/label="SV40pro_F_primer"
promoter complement(2029..2047)
/label="T7_promoter"
promoter complement(2057..2073)
/label="M13_forward20_primer"
misc_feature complement(2066..2088)
/label="M13_pUC_fwd_primer"
misc_feature 2066..2214
/label="lacZ_a"
rep_origin 2232..2538
/label="f1_origin"
promoter 2731..2759
/label="AmpR_promoter"
gene 2801..3661
/label="Ampicillin"
/gene="Ampicillin"
CDS 2801..3661
/label="ORF frame 2"
rep_origin 3816..4435
/label="pBR322_origin"
promoter 4744..4773
/label="lac_promoter"
misc_feature 4787..4809
/label="M13_pUC_rev_primer"
promoter 4808..4826
/label="M13_reverse_primer"
promoter 4843..4862
/label="T3_promoter"
misc_feature 5116..5296
/label="HIV-1_5_LTR"
misc_feature 5116..5296
/label="truncHIV-1_3_LTR"
misc_feature 5407..5451
/label="HIV-1_psi_pack"
CDS 5839..7026
/label="ORF frame 1"
misc_feature 5961..6194
/label="RRE"
misc_feature 6694..6709
/label="cPPT"
promoter 6826..7330
/label="CMV_immearly_promoter"
misc_feature 6833..7120
/label="CAG_enhancer"
misc_feature 7287..7307
/label="CMV_fwd_primer"
promoter 7288..7357
/label="CMV_promoter"
misc_feature 7331..7350
/label="pCEP_fwd_primer"
misc_feature 7333..7357
/label="LNCX_primer"
ORIGIN
1 gatccgccgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
61 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
181 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
241 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
301 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
361 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
481 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
541 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
601 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
661 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
721 agtaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
781 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
841 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
901 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
961 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
1021 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
1081 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
1141 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
1201 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
1261 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
1321 ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
1381 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
1441 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
1501 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
1561 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
1621 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
1681 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
1741 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
1801 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
1861 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
1921 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
1981 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
2041 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
2101 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
2161 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
2221 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
2281 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
2341 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
2401 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
2461 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
2521 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
2581 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
2641 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
2701 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
2761 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
2821 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
2881 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
2941 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
3001 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
3061 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
3121 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
3181 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
3241 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
3301 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
3361 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
3421 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
3481 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
3541 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
3601 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
3661 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
3721 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
3781 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
3841 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
3901 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
3961 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
4021 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
4081 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
4141 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
4201 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
4261 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
4321 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
4381 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
4441 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
4501 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
4561 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
4621 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
4681 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
4741 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
4801 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
4861 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
4921 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
4981 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
5041 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
5101 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
5161 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
5221 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
5281 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
5341 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
5401 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
5461 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
5521 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
5581 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
5641 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
5701 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
5761 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
5821 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
5881 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
5941 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
6001 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
6061 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
6121 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
6181 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
6241 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
6301 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
6361 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
6421 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
6481 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
6541 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
6601 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
6661 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
6721 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
6781 aaacaaatta caaaattcaa aattttatcg ataagcttgg gagttccgcg ttacataact
6841 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
6901 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
6961 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
7021 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
7081 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
7141 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
7201 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
7261 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
7321 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
7381 ttttgacctc catagaagac accgactcta gag
//
