pRS413

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Stratagene(Agilent)
载体名称: pRS413
质粒类型: 酿酒酵母载体;酵母着丝粒质粒;穿梭载体
克隆方法: 多克隆位点,限制性内切酶
高拷贝/低拷贝: 单拷贝
启动子: --
载体大小: 4970 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素Ampicillin
筛选标记: --
克隆宿主: XL1-Blue
备注: 含a beta-gal报告基因;宿主菌株YPH499,YPH500,YPH501
产品目录号: --
稳定性: --
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pRS413载体图谱



载体简介

载体序列

pRS413, 4970 bp                               version 013001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 

   1  TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG  
  51  GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG  
 101  TCAGGGCGCG TCAGCGCGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG  
 151  CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAATT CCCGTTTTAA  
 201  GAGCTTGGTG AGCGCTAGGA GTCACTGCCA GGTATCGTTT GAACACGGCA  
 251  TTAGTCAGGG AAGTCATAAC ACAGTCCTTT CCCGCAATTT TCTTTTTCTA  
 301  TTACTCTTGG CCTCCTCTAG TACACTCTAT ATTTTTTTAT GCCTCGGTAA  
 351  TGATTTTCAT TTTTTTTTTT CCCCTAGCGG ATGACTCTTT TTTTTTCTTA  
 401  GCGATTGGCA TTATCACATA ATGAATTATA CATTATATAA AGTAATGTGA  
 451  TTTCTTCGAA GAATATACTA AAAAATGAGC AGGCAAGATA AACGAAGGCA  
 501  AAGATGACAG AGCAGAAAGC CCTAGTAAAG CGTATTACAA ATGAAACCAA  
 551  GATTCAGATT GCGATCTCTT TAAAGGGTGG TCCCCTAGCG ATAGAGCACT  
 601  CGATCTTCCC AGAAAAAGAG GCAGAAGCAG TAGCAGAACA GGCCACACAA  
 651  TCGCAAGTGA TTAACGTCCA CACAGGTATA GGGTTTCTGG ACCATATGAT  
 701  ACATGCTCTG GCCAAGCATT CCGGCTGGTC GCTAATCGTT GAGTGCATTG  
 751  GTGACTTACA CATAGACGAC CATCACACCA CTGAAGACTG CGGGATTGCT  
 801  CTCGGTCAAG CTTTTAAAGA GGCCCTACTG GCGCGTGGAG TAAAAAGGTT  
 851  TGGATCAGGA TTTGCGCCTT TGGATGAGGC ACTTTCCAGA GCGGTGGTAG  
 901  ATCTTTCGAA CAGGCCGTAC GCAGTTGTCG AACTTGGTTT GCAAAGGGAG  
 951  AAAGTAGGAG ATCTCTCTTG CGAGATGATC CCGCATTTTC TTGAAAGCTT  
1001  TGCAGAGGCT AGCAGAATTA CCCTCCACGT TGATTGTCTG CGAGGCAAGA  
1051  ATGATCATCA CCGTAGTGAG AGTGCGTTCA AGGCTCTTGC GGTTGCCATA  
1101  AGAGAAGCCA CCTCGCCCAA TGGTACCAAC GATGTTCCCT CCACCAAAGG  
1151  TGTTCTTATG TAGTGACACC GATTATTTAA AGCTGCAGCA TACGATATAT  
1201  ATACATGTGT ATATATGTAT ACCTATGAAT GTCAGTAAGT ATGTATACGA  
1251  ACAGTATGAT ACTGAAGATG ACAAGGTAAT GCATCATTCT ATACGTGTCA  
1301  TTCTGAACGA GGCGCGCTTT CCTTTTTTCT TTTTGCTTTT TCTTTTTTTT  
1351  TCTCTTGAAC TCGACGGATC TATGCGGTGT GAAATACCGC ACAGATGCGT  
1401  AAGGAGAAAA TACCGCATCA GGAAATTGTA AACGTTAATA TTTTGTTAAA  
1451  ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG  
1501  AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG  
1551  AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC  
1601  CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG  
1651  AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA  
1701  AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC  
1751  GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA  
1801  GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC  
1851  GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT  
1901  GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC  
1951  AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA  
2001  GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCGTA  
2051  ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC CTCGAGGTCG  
2101  ACGGTATCGA TAAGCTTGAT ATCGAATTCC TGCAGCCCGG GGGATCCACT  
2151  AGTTCTAGAG CGGCCGCCAC CGCGGTGGAG CTCCAGCTTT TGTTCCCTTT  
2201  AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT  
2251  GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAG GAGCCGGAAG  
2301  CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGGTAA CTCACATTAA  
2351  TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG  
2401  CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG  
2451  GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC  
2501  TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA  
2551  GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA  
2601  GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC  
2651  GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA  
2701  AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT  
2751  CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT  
2801  TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT  
2851  CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC  
2901  CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT  
2951  CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC  
3001  AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG  
3051  GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC  
3101  TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC  
3151  AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT  
3201  TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG  
3251  GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG  
3301  AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG  
3351  TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC  
3401  AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA  
3451  TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG  
3501  CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC  
3551  CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC  
3601  AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG  
3651  CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG  
3701  TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT  
3751  TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT  
3801  GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA  
3851  GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT  
3901  TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA  
3951  CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT  
4001  GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA  
4051  GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT  
4101  ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT  
4151  CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA  
4201  AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT  
4251  ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT  
4301  GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA  
4351  GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGGGT CCTTTTCATC  
4401  ACGTGCTATA AAAATAATTA TAATTTAAAT TTTTTAATAT AAATATATAA  
4451  ATTAAAAATA GAAAGTAAAA AAAGAAATTA AAGAAAAAAT AGTTTTTGTT  
4501  TTCCGAAGAT GTAAAAGACT CTAGGGGGAT CGCCAACAAA TACTACCTTT  
4551  TATCTTGCTC TTCCTGCTCT CAGGTATTAA TGCCGAATTG TTTCATCTTG  
4601  TCTGTGTAGA AGACCACACA CGAAAATCCT GTGATTTTAC ATTTTACTTA  
4651  TCGTTAATCG AATGTATATC TATTTAATCT GCTTTTCTTG TCTAATAAAT  
4701  ATATATGTAA AGTACGCTTT TTGTTGAAAT TTTTTAAACC TTTGTTTATT  
4751  TTTTTTTCTT CATTCCGTAA CTCTTCTACC TTCTTTATTT ACTTTCTAAA  
4801  ATCCAAATAC AAAACATAAA AATAAATAAA CACAGAGTAA ATTCCCAAAT  
4851  TATTCCATCA TTAAAAGATA CGAGGCGCGT GTAAGTTACA GGCAAGCGAT  
4901  CCGTCCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG  
4951  TATCACGAGG CCCTTTCGTC 

载体质粒图谱pdf版和相关资料下载

载体应用举例

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