pLZ415

LOCUS       pLZ415             8859 bp DNA     circular SYN 10-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=oMHS-CMV-F
     misc_feature    829
                     /label=putative transcriptional start
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_recomb     905..938
                     /label=LoxP
     regulatory      1001..1010
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1007..1069
                     /label=Igk secretion signal
     CDS             1076..1663
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     misc_feature    1670..2413
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     primer_bind     1673..1694
                     /label=193-WKG-615-F
     misc_feature    1943..1945
                     /label=F93A
                     /note="F93A"
     misc_feature    2397..2413
                     /label=EpoR-D1_D2_TM
     misc_feature    2423..3251
                     /label=IL6ST
     misc_feature    2450..2476
                     /label=box1
     misc_feature    2774..2776
                     /label=Y759A
     polyA_signal    3280..3504
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      3513..3697
                     /label=SV40 late polyA
     protein_bind    complement(3734..3767)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3780..3848
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
     CDS             3867..4379
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    3900..3902
                     /label=cyt-STOP (TGG->TGA)
     terminator      4386..4408
                     /label=SV40 late polyA
     polyA_signal    4456..4680
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4726..5154
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     primer_bind     complement(4740..4764)
                     /label=184-WKG-599-R
     primer_bind     5025..5044
                     /label=189-WKG-612-F
     promoter        5168..5497
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    5400..5449
                     /label=P_SV40 TSS-region
     misc_feature    5464..5469
                     /label=SDS
     misc_feature    5564..6355
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     polyA_signal    6532..6653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6698..6718)
                     /label=M13-rev
     primer_bind     complement(6702..6718)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(6724..6746)
                     /label=LacO
     protein_bind    6726..6742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6750..6780)
                     /label=lac promoter
     primer_bind     complement(6763..6786)
                     /label=190-WKG-612-R
     protein_bind    6795..6816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6922..7862
                     /label=ColE1
                     /note="ColE1"
     misc_feature    complement(7866..8723)
                     /label=AmpR
                     /label=nonstandard type: resistance
     promoter        complement(8724..8828)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gcttataact tcgtatagca tacattatac gaagttatcg gcgcgccgcg gccgcgatca
      961 attcggtacc gaagttccta ttccgaattc ctagtggccg gccaccatgg agaccgatac
     1021 cttattactg tgggtgcttc ttctgtgggt tcccggtagc accggagacg gatccaagaa
     1081 gaagggcagc gtggtgatcg tgggcagaat caacctgagc ggcgacaccg cctacagcca
     1141 gcagaccaga ggcctggagg gctgccagga gaccagccag accggcagag acaagaacca
     1201 ggtggagggc gaggtgcagg tggtgagcac cgccacccag agcttcctgg ccaccagcat
     1261 caacggcgtg ctgtggaccg tgtaccacgg cgccggcacc agaaccatcg ccagccccaa
     1321 gggccccgtg acccagatgt acacgaacgt ggacaaggac ttagtgggct ggcaggcccc
     1381 ccaaggcagc agaagcctga ccccctgcac ctgcggcagc agcgacctgt acctggtgac
     1441 cagacacgcc gacgtgatcc ccgtgagaag aagaggcgac agcagaggca gcctgctgag
     1501 ccccagaccc atcagctatc tgaaaggctc aagcggcggc cccctgttgt gtcccgccgg
     1561 ccatgccgtg ggcatcttca gagccgccgt gagcaccaga ggcgtggcca aggccgtgga
     1621 cttcatcccc gtggagagcc tggagaccac catgagaagc cccgaattcg caccttcacc
     1681 cagcctcccg gaccccaagt ttgagagcaa agcggccctg ctggcatccc ggggctccga
     1741 agaacttctg tgcttcaccc aacgcttgga agacttggtg tgtttctggg aggaagcggc
     1801 gagctccggg atggacttca actacagctt ctcataccag ctcgagggtg agtcacgaaa
     1861 gtcatgtagc ctgcaccagg ctcccaccgt ccgcggctcc gtgcgtttct ggtgttcact
     1921 gccaacagcg gacacatcga gtgctgtgcc gctggagctg caggtgacgg aggcgtccgg
     1981 ttctcctcgc tatcaccgca tcatccatat caatgaagta gtgctcctgg acgcccccgc
     2041 ggggctgctg gcgcgccggg cagaagaggg cagccacgtg gtgctgcgct ggctgccacc
     2101 tcctggagca cctatgacca cccacatccg atatgaagtg gacgtgtcgg caggcaaccg
     2161 ggcaggaggg acacaaaggg tggaggtcct ggaaggccgc actgagtgtg ttctgagcaa
     2221 cctgcggggc gggacgcgct acaccttcgc tgttcgagcg cgcatggccg agccgagctt
     2281 cagcggattc tggagtgcct ggtctgagcc cgcgtcacta ctgaccgcta gcgacctgga
     2341 ccctctcatc ttgacgctgt ctctcattct ggtcctcatc tcgttgttgc tgacggttct
     2401 ggccctgctg tccgcagcag caaataagcg agacctaatt aaaaaacaca tctggcctaa
     2461 tgttccagat ccttcaaaga gtcatattgc ccagtggtca cctcacactc ctccaaggca
     2521 caattttaat tcaaaagatc aaatgtattc agatggcaat ttcactgatg taagtgttgt
     2581 ggaaatagaa gcaaatgaca aaaagccttt tccagaagat ctgaaatcat tggacctgtt
     2641 caaaaaggaa aaaattaata ctgaaggaca cagcagtggt attggggggt cttcatgcat
     2701 gtcatcttct aggccaagca tttctagcag tgatgaaaat gaatcttcac aaaacacttc
     2761 gagcactgtc caggcttcta ccgtggtaca cagtggctac agacaccaag ttccgtcagt
     2821 ccaagtcttc tcaagatccg agtctaccca gcccttgtta gattcagagg agcggccaga
     2881 agatctacaa ttagtagatc atgtagatgg cggtgatggt atttttccca ggcaacagta
     2941 cttcaaacag aactgcagtc agcatgaatc cagtccagat atttcacatt ttgaaaggtc
     3001 aaagcaagtt tcatcagtca atgaggaaga ttttgttaga cttaaacagc agatttcaga
     3061 tcatatttca caatcctgtg gatctgggca aatgaaaatg tttcaggaag tttctgcagc
     3121 agatgctttt ggtccaggta ctgagggaca agtagaaaga tttgaaacag ttggcatgga
     3181 ggctgcgact gatgaaggca tgcctaaaag ttacttacca cagactgtac ggcaaggcgg
     3241 ctacatgcct cagtaacctg cagggctgat cagcctcgac tgtgccttct agttgccagc
     3301 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3361 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3421 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3481 ctggggatgc ggtgggctct atggcttctg aggataagat acattgatga gtttggacaa
     3541 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3601 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3661 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcgcttggta ccgtctttcc
     3721 agtggttaat taaataactt cgtatagcat acattatacg aagttatctc gaggccacca
     3781 tgactagtga gacagacaca ctcctgctat gggtactgct gctctgggtt ccaggttcca
     3841 ctggtgacgc tagtggtggt tctggtatgg tcttcacact cgaagatttc gttggggact
     3901 ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt gtgtccagtt
     3961 tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg agcggtgaaa
     4021 atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc ggcgaccaaa
     4081 tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat cactttaagg
     4141 tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg atcgactatt
     4201 tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact gtaacaggga
     4261 ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac ggctccctgc
     4321 tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc attctggcga
     4381 tcgatagaca tgataagata cattgatgtc tagaagcttc tagagggccc gtttaaaccc
     4441 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4501 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4561 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4621 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4681 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     4741 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4801 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4861 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4921 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4981 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5041 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5101 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     5161 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5221 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5281 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5341 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5401 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     5461 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     5521 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5581 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5641 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5701 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     5761 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5821 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5881 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5941 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6001 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6061 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     6121 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6181 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6241 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6301 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6361 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6421 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6481 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6661 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6721 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6781 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6841 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6901 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7501 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7861 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7921 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7981 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8041 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8161 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8281 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8701 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8821 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//