| 质粒名称: | pDA083 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 131389 |
| 存储实验室: | Kathleen Burns |
|
载体骨架基本信息 |
|
| 载体名称: | pCEP4 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 新霉素替代潮霉素 |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | eGFP |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pDA083 was a gift from Kathleen Burns (Addgene plasmid # 131389 ; http://n2t.net/addgene:131389 ; RRID:Addgene_131389) |
| 参考文献部分: | Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication. Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, Smogorzewska A, Holland AJ, Burns KH. Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 10.1038/s41594-020-0372-1. Epub 2020 Feb 10. 10.1038/s41594-020-0372-1 PubMed 32042151 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDA083 10004 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION eGFP expression.
ACCESSION .
VERSION .
KEYWORDS pDA083
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10004)
AUTHORS Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH,
Smogorzewska A, Holland AJ, Burns KH
TITLE Cell fitness screens reveal a conflict between LINE-1
retrotransposition and DNA replication.
JOURNAL Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi:
10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
PUBMED 32042151
REFERENCE 2 (bases 1 to 10004)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10004
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 581..605
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 643..652
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 649..1365
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 649..1365
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(694..715)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(955..974)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1302..1323
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 1412..1546
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1436..1455)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1490..1509
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(1689..1711)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6038..6056)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6124..6228
/gene="bla"
/label=AmpR promoter
CDS 6229..7089
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6447..6466)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7260..7848
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7749..7768
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 8279..8424
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
CDS 8467..9261
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8521..8540)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9131..9150
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 9303..9351
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
601 agatctctag aagctgggta ccagctgcta gcgctaccgg tcgccaccat ggtgagcaag
661 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
721 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
781 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
841 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
901 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
961 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
1021 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
1081 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
1141 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
1201 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
1261 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
1321 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct
1381 cgagctcaag cttcgaatag cggccgctaa ggatccagac atgataagat acattgatga
1441 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
1501 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
1561 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
1621 cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac
1681 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
1741 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
1801 gccatgaggt cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga
1861 catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact
1921 tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc
1981 tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc
2041 atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact
2101 cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga
2161 ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc
2221 ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc
2281 atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt
2341 tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc
2401 gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag
2461 ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac
2521 tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg
2581 agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc
2641 agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc
2701 tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa
2761 caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat
2821 ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg
2881 tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga
2941 aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt
3001 aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg
3061 aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta
3121 aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact
3181 tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca
3241 agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc
3301 caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg
3361 ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
3421 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
3481 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
3541 tctgggtagc atatgctatc ctaatmkaka tywgggtagt atatgctatc ctaatttata
3601 tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
3661 ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
3721 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
3781 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
3841 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca
3901 gtcagcatat gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct
3961 cccaaggggg cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag
4021 gtgaatttaa ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt
4081 cgctaatgtt ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg
4141 ggtatgccca attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa
4201 atacaccaac agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca
4261 cggcttttaa tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca
4321 ccctcatctc catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc
4381 ccttccacca taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca
4441 gtcaccctga tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc
4501 ttcaaaacct cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac
4561 tcccttagcg ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc
4621 aatggtgtaa gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg
4681 gaggtcttac tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc
4741 ctttctacgt gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc
4801 gggttggaac ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct
4861 ccatcaccct gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc
4921 tgctctatcg ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat
4981 tcaaaataat cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg
5041 cgcggtggag acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca
5101 cgtccacgac ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct
5161 ctaccccggc ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg
5221 cctccactgc ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc
5281 cctcctcctg ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt
5341 ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct ccggacacca
5401 tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc gcctcctcgt
5461 cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg aggtccactg
5521 ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt tccaggtcct
5581 gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac atctttatta
5641 gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag cccccccacc
5701 ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc catcctccga
5761 accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct gaacatcctc
5821 aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct ttttgctgga
5881 cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac cagacagaga
5941 tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc ttatcgatga
6001 taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
6061 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
6121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
6181 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
6241 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
6301 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
6361 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
6421 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
6481 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
6541 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
6601 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
6661 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
6721 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
6781 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
6841 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
6901 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
6961 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
7021 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
7081 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
7141 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
7201 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
7261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
7321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
7381 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
7441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
7501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
7561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
7621 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
7681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
7741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
7801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
7861 gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg atggtcgtca
7921 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
7981 agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc
8041 agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
8101 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
8161 ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg tggcccgttg
8221 ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat
8281 gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg
8341 gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga
8401 gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa
8461 tgagatatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
8521 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
8581 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
8641 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
8701 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
8761 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
8821 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
8881 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
8941 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
9001 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
9061 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
9121 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
9181 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
9241 cttcttgacg agttcttcta aaacacggaa ggagacaata ccggaaggaa cccgcgctat
9301 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
9361 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
9421 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
9481 tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg ggttagggac
9541 ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg gcgttgcgtg
9601 gggtcaggtc cacgactgga ctgagcagac agacccatgg tttttggatg gcctgggcat
9661 ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa acacccccga
9721 cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct agtcgaccaa ttctcatgtt
9781 tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg
9841 gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg
9901 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa
9961 tatgtacatt tatattggct catgtccaat atgaccgcca tgtt
//
