pAdEasy-2

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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pAdEasy2载体基本信息

质粒名称: pAdEasy-2 
出品公司: 爱迪基因
目录编号: 16401
存储实验室: Bert Vogelstein

载体骨架基本信息
载体名称: pAdEasy-2
载体出品公司: --
载体抗性: 氨苄青霉素
空载体大小: 30767 bp
完整质粒大小: --
载体修饰: --
载体类型: 哺乳动物表达,腺病毒
筛选标记: --
高拷贝/低拷贝: 低拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: --
物种来源: --
插入大小: --
基因突变: --
GenBank编号 AY370909
启动子 --
标签/融合蛋白: --
克隆方法: 限制性内切酶
5' 克隆位点: --
3' 克隆位点: --
5' 测序引物 --
3' 测序引物 --

文献引用方法
材料和方法部分: pAdEasy-2 was a gift from Bert Vogelstein (Addgene plasmid # 16401 ; http://n2t.net/addgene:16401 ; RRID:Addgene_16401)
参考文献部分: A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916

pAdEasy2载体图谱质粒图谱和多克隆位点信息

pAdEasy-2质粒图谱

pAdEasy2载体简介

pAdEasy2载体序列

LOCUS       pAdEasy-2               30829 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Adenoviral vector for use in AdEasy System, for use with large 
            inserts..
ACCESSION   .
VERSION     .
KEYWORDS    pAdEasy-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 30829)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 30829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..30829
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3262..3365
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             complement(10458..10469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      17941..17950
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     repeat_region   26763..26865
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     CDS             28156..28347
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(28341..28363)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    28449..28589
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     28504..28523
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(28604..28621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(28775..29363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(28855..28874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(29534..30394)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     30157..30176
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(30395..30499)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctc cgccgccgct tcagccgctg cagccaccgc ccgcgggatt gtgactgact
       61 ttgctttcct gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc cgcgatgaca
      121 agttgacggc tcttttggca caattggatt ctttgacccg ggaacttaat gtcgtttctc
      181 agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc cctcccaatg
      241 cggtttaaaa cataaataaa aaaccagact ctgtttggat ttggatcaag caagtgtctt
      301 gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg tctcggtcgt
      361 tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg actctggatg ttcagataca
      421 tgggcataag cccgtctctg gggtggaggt agcaccactg cagagcttca tgctgcgggg
      481 tggtgttgta gatgatccag tcgtagcagg agcgctgggc gtggtgccta aaaatgtctt
      541 tcagtagcaa gctgattgcc aggggcaggc ccttggtgta agtgtttaca aagcggttaa
      601 gctgggatgg gtgcatacgt ggggatatga gatgcatctt ggactgtatt tttaggttgg
      661 ctatgttccc agccatatcc ctccggggat tcatgttgtg cagaaccacc agcacagtgt
      721 atccggtgca cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg aagaacttgg
      781 agacgccctt gtgacctcca agattttcca tgcattcgtc cataatgatg gcaatgggcc
      841 cacgggcggc ggcctgggcg aagatatttc tgggatcact aacgtcatag ttgtgttcca
      901 ggatgagatc gtcataggcc atttttacaa agcgcgggcg gagggtgcca gactgcggta
      961 taatggttcc atccggccca ggggcgtagt taccctcaca gatttgcatt tcccacgctt
     1021 tgagttcaga tggggggatc atgtctacct gcggggcgat gaagaaaacg gtttccgggg
     1081 taggggagat cagctgggaa gaaagcaggt tcctgagcag ctgcgactta ccgcagccgg
     1141 tgggcccgta aatcacacct attaccggct gcaactggta gttaagagag ctgcagctgc
     1201 cgtcatccct gagcaggggg gccacttcgt taagcatgtc cctgactcgc atgttttccc
     1261 tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag cagttcttgc aaggaagcaa
     1321 agtttttcaa cggtttgaga ccgtccgccg taggcatgct tttgagcgtt tgaccaagca
     1381 gttccaggcg gtcccacagc tcggtcacct gctctacggc atctcgatcc agcatatctc
     1441 ctcgtttcgc gggttggggc ggctttcgct gtacggcagt agtcggtgct cgtccagacg
     1501 ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct gggtcacggt
     1561 gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg tcctgctggt
     1621 gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg tagcatttga ccatggtgtc
     1681 atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg aggaggcgcc
     1741 gcacgagggg cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa ataccgattc
     1801 cggggagtag gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca cgagccaggt
     1861 gagctctggc cgttcggggt caaaaaccag gtttccccca tgctttttga tgcgtttctt
     1921 acctctggtt tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt ccgtgtcccc
     1981 gtatacagac ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct cgtatagaaa
     2041 ctcggaccac tctgagacaa aggctcgcgt ccaggccagc acgaaggagg ctaagtggga
     2101 ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa gacacatgtc
     2161 gccctcttcg gcatcaagga aggtgattgg tttgtaggtg taggccacgt gaccgggtgt
     2221 tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct cttccgcatc
     2281 gctgtctgcg agggccagct gttggggtga gtactccctc tgaaaagcgg gcatgacttc
     2341 tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt
     2401 gatgcctttg agggtggccg catccatctg gtcagaaaag acaatctttt tgttgtcaag
     2461 cttggtggca aacgacccgt agagggcgtt ggacagcaac ttggcgatgg agcgcagggt
     2521 ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca cgtattcgcg
     2581 cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca ggtgcacgcg
     2641 ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc cgcgtaggcg
     2701 ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag aatggcggta gggggtctag
     2761 ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc ccgggcagca ggcgcgcgtc
     2821 gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc gggcggcaag
     2881 cgcgcgctcg tatgggttga gtgggggacc ccatggcatg gggtgggtga gcgcggaggc
     2941 gtacatgccg caaatgtcgt aaacgtagag gggctctctg agtattccaa gatatgtagg
     3001 gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt gcgagggagc
     3061 gaggaggtcg ggaccgaggt tgctacgggc gggctgctct gctcggaaga ctatctgcct
     3121 gaagatggca tgtgagttgg atgatatggt tggacgctgg aagacgttga agctggcgtc
     3181 tgtgagacct accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct tgttgaccag
     3241 ctcggcggtg acctgcacgt ctagggcgca gtagtccagg gtttccttga tgatgtcata
     3301 cttatcctgt cccttttttt tccacagctc gcggttgagg acaaactctt cgcggtcttt
     3361 ccagtactct tggatcggaa acccgtcggc ctccgaacgg taagagccta gcatgtagaa
     3421 ctggttgacg gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt atgcctgcgc
     3481 ggccttccgg agcgaggtgt gggtgagcgc aaaggtgtcc ctgaccatga ctttgaggta
     3541 ctggtatttg aagtcagtgt cgtcgcatcc gccctgctcc cagagcaaaa agtccgtgcg
     3601 ctttttggaa cgcggatttg gcagggcgaa ggtgacatcg ttgaagagta tctttcccgc
     3661 gcgaggcata aagttgcgtg tgatgcggaa gggtcccggc acctcggaac ggttgttaat
     3721 tacctgggcg gcgagcacga tctcgtcaaa gccgttgatg ttgtggccca caatgtaaag
     3781 ttccaagaag cgcgggatgc ccttgatgga aggcaatttt ttaagttcct cgtaggtgag
     3841 ctcttcaggg gagctgagcc cgtgctctga aagggcccag tctgcaagat gagggttgga
     3901 agcgacgaat gagctccaca ggtcacgggc cattagcatt tgcaggtggt cgcgaaaggt
     3961 cctaaactgg cgacctatgg ccattttttc tggggtgatg cagtagaagg taagcgggtc
     4021 ttgttcccag cggtcccatc caaggttcgc ggctaggtct cgcgcggcag tcactagagg
     4081 ctcatctccg ccgaacttca tgaccagcat gaagggcacg agctgcttcc caaaggcccc
     4141 catccaagta taggtctcta catcgtaggt gacaaagaga cgctcggtgc gaggatgcga
     4201 gccgatcggg aagaactgga tctcccgcca ccaattggag gagtggctat tgatgtggtg
     4261 aaagtagaag tccctgcgac gggccgaaca ctcgtgctgg cttttgtaaa aacgtgcgca
     4321 gtactggcag cggtgcacgg gctgtacatc ctgcacgagg ttgacctgac gaccgcgcac
     4381 aaggaagcag agtgggaatt tgagcccctc gcctggcggg tttggctggt ggtcttctac
     4441 ttcggctgct tgtccttgac cgtctggctg ctcgagggga gttacggtgg atcggaccac
     4501 cacgccgcgc gagcccaaag tccagatgtc cgcgcgcggc ggtcggagct tgatgacaac
     4561 atcgcgcaga tgggagctgt ccatggtctg gagctcccgc ggcgtcaggt caggcgggag
     4621 ctcctgcagg tttacctcgc atagacgggt cagggcgcgg gctagatcca ggtgatacct
     4681 aatttccagg ggctggttgg tggcggcgtc gatggcttgc aagaggccgc atccccgcgg
     4741 cgcgactacg gtaccgcgcg gcgggcggtg ggccgcgggg gtgtccttgg atgatgcatc
     4801 taaaagcggt gacgcgggcg agcccccgga ggtagggggg gctccggacc cgccgggaga
     4861 gggggcaggg gcacgtcggc gccgcgcgcg ggcaggagct ggtgctgcgc gcgtaggttg
     4921 ctggcgaacg cgacgacgcg gcggttgatc tcctgaatct ggcgcctctg cgtgaagacg
     4981 acgggcccgg tgagcttgaa cctgaaagag agttcgacag aatcaatttc ggtgtcgttg
     5041 acggcggcct ggcgcaaaat ctcctgcacg tctcctgagt tgtcttgata ggcgatctcg
     5101 gccatgaact gctcgatctc ttcctcctgg agatctccgc gtccggctcg ctccacggtg
     5161 gcggcgaggt cgttggaaat gcgggccatg agctgcgaga aggcgttgag gcctccctcg
     5221 ttccagacgc ggctgtagac cacgccccct tcggcatcgc gggcgcgcat gaccacctgc
     5281 gcgagattga gctccacgtg ccgggcgaag acggcgtagt ttcgcaggcg ctgaaagagg
     5341 tagttgaggg tggtggcggt gtgttctgcc acgaagaagt acataaccca gcgtcgcaac
     5401 gtggattcgt tgatatcccc caaggcctca aggcgctcca tggcctcgta gaagtccacg
     5461 gcgaagttga aaaactggga gttgcgcgcc gacacggtta actcctcctc cagaagacgg
     5521 atgagctcgg cgacagtgtc gcgcacctcg cgctcaaagg ctacaggggc ctcttcttct
     5581 tcttcaatct cctcttccat aagggcctcc ccttcttctt cttctggcgg cggtggggga
     5641 ggggggacac ggcggcgacg acggcgcacc gggaggcggt cgacaaagcg ctcgatcatc
     5701 tccccgcggc gacggcgcat ggtctcggtg acggcgcggc cgttctcgcg ggggcgcagt
     5761 tggaagacgc cgcccgtcat gtcccggtta tgggttggcg gggggctgcc atgcggcagg
     5821 gatacggcgc taacgatgca tctcaacaat tgttgtgtag gtactccgcc gccgagggac
     5881 ctgagcgagt ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc taaccagtca
     5941 cagtcgcaag gtaggctgag caccgtggcg ggcggcagcg ggcggcggtc ggggttgttt
     6001 ctggcggagg tgctgctgat gatgtaatta aagtaggcgg tcttgagacg gcggatggtc
     6061 gacagaagca ccatgtcctt gggtccggcc tgctgaatgc gcaggcggtc ggccatgccc
     6121 caggcttcgt tttgacatcg gcgcaggtct ttgtagtagt cttgcatgag cctttctacc
     6181 ggcacttctt cttctccttc ctcttgtcct gcatctcttg catctatcgc tgcggcggcg
     6241 gcggagtttg gccgtaggtg gcgccctctt cctcccatgc gtgtgacccc gaagcccctc
     6301 atcggctgaa gcagggctag gtcggcgaca acgcgctcgg ctaatatggc ctgctgcacc
     6361 tgcgtgaggg tagactggaa gtcatccatg tccacaaagc ggtggtatgc gcccgtgttg
     6421 atggtgtaag tgcagttggc cataacggac cagttaacgg tctggtgacc cggctgcgag
     6481 agctcggtgt acctgagacg cgagtaagcc ctcgagtcaa atacgtagtc gttgcaagtc
     6541 cgcaccaggt actggtatcc caccaaaaag tgcggcggcg gctggcggta gaggggccag
     6601 cgtagggtgg ccggggctcc gggggcgaga tcttccaaca taaggcgatg atatccgtag
     6661 atgtacctgg acatccaggt gatgccggcg gcggtggtgg aggcgcgcgg aaagtcgcgg
     6721 acgcggttcc agatgttgcg cagcggcaaa aagtgctcca tggtcgggac gctctggccg
     6781 gtcaggcgcg cgcaatcgtt gacgctctag cgtgcaaaag gagagcctgt aagcgggcac
     6841 tcttccgtgg tctggtggat aaattcgcaa gggtatcatg gcggacgacc ggggttcgag
     6901 ccccgtatcc ggccgtccgc cgtgatccat gcggttaccg cccgcgtgtc gaacccaggt
     6961 gtgcgacgtc agacaacggg ggagtgctcc ttttggcttc cttccaggcg cggcggctgc
     7021 tgcgctagct tttttggcca ctggccgcgc gcagcgtaag cggttaggct ggaaagcgaa
     7081 agcattaagt ggctcgctcc ctgtagccgg agggttattt tccaagggtt gagtcgcggg
     7141 acccccggtt cgagtctcgg accggccgga ctgcggcgaa cgggggtttg cctccccgtc
     7201 atgcaagacc ccgcttgcaa attcctccgg aaacagggac gagccccttt tttgcttttc
     7261 ccagatgcat ccggtgctgc ggcagatgcg cccccctcct cagcagcggc aagagcaaga
     7321 gcagcggcag acatgcaggg caccctcccc tcctcctacc gcgtcaggag gggcgacatc
     7381 cgcggttgac gcggcagcag atggtgatta cgaacccccg cggcgccggg cccggcacta
     7441 cctggacttg gaggagggcg agggcctggc gcggctagga gcgccctctc ctgagcggca
     7501 cccaagggtg cagctgaagc gtgatacgcg tgaggcgtac gtgccgcggc agaacctgtt
     7561 tcgcgaccgc gagggagagg agcccgagga gatgcgggat cgaaagttcc acgcagggcg
     7621 cgagctgcgg catggcctga atcgcgagcg gttgctgcgc gaggaggact ttgagcccga
     7681 cgcgcgaacc gggattagtc ccgcgcgcgc acacgtggcg gccgccgacc tggtaaccgc
     7741 atacgagcag acggtgaacc aggagattaa ctttcaaaaa agctttaaca accacgtgcg
     7801 tacgcttgtg gcgcgcgagg aggtggctat aggactgatg catctgtggg actttgtaag
     7861 cgcgctggag caaaacccaa atagcaagcc gctcatggcg cagctgttcc ttatagtgca
     7921 gcacagcagg gacaacgagg cattcaggga tgcgctgcta aacatagtag agcccgaggg
     7981 ccgctggctg ctcgatttga taaacatcct gcagagcata gtggtgcagg agcgcagctt
     8041 gagcctggct gacaaggtgg ccgccatcaa ctattccatg cttagcctgg gcaagtttta
     8101 cgcccgcaag atataccata ccccttacgt tcccatagac aaggaggtaa agatcgaggg
     8161 gttctacatg cgcatggcgc tgaaggtgct taccttgagc gacgacctgg gcgtttatcg
     8221 caacgagcgc atccacaagg ccgtgagcgt gagccggcgg cgcgagctca gcgaccgcga
     8281 gctgatgcac agcctgcaaa gggccctggc tggcacgggc agcggcgata gagaggccga
     8341 gtcctacttt gacgcgggcg ctgacctgcg ctgggcccca agccgacgcg ccctggaggc
     8401 agctggggcc ggacctgggc tggcggtggc acccgcgcgc gctggcaacg tcggcggcgt
     8461 ggaggaatat gacgaggacg atgagtacga gccagaggac ggcgagtact aagcggtgat
     8521 gtttctgatc agatgatgca agacgcaacg gacccggcgg tgcgggcggc gctgcagagc
     8581 cagccgtccg gccttaactc cacggacgac tggcgccagg tcatggaccg catcatgtcg
     8641 ctgactgcgc gcaatcctga cgcgttccgg cagcagccgc aggccaaccg gctctccgca
     8701 attctggaag cggtggtccc ggcgcgcgca aaccccacgc acgagaaggt gctggcgatc
     8761 gtaaacgcgc tggccgaaaa cagggccatc cggcccgacg aggccggcct ggtctacgac
     8821 gcgctgcttc agcgcgtggc tcgttacaac agcggcaacg tgcagaccaa cctggaccgg
     8881 ctggtggggg atgtgcgcga ggccgtggcg cagcgtgagc gcgcgcagca gcagggcaac
     8941 ctgggctcca tggttgcact aaacgccttc ctgagtacac agcccgccaa cgtgccgcgg
     9001 ggacaggagg actacaccaa ctttgtgagc gcactgcggc taatggtgac tgagacaccg
     9061 caaagtgagg tgtaccagtc tgggccagac tattttttcc agaccagtag acaaggcctg
     9121 cagaccgtaa acctgagcca ggctttcaaa aacttgcagg ggctgtgggg ggtgcgggct
     9181 cccacaggcg accgcgcgac cgtgtctagc ttgctgacgc ccaactcgcg cctgttgctg
     9241 ctgctaatag cgcccttcac ggacagtggc agcgtgtccc gggacacata cctaggtcac
     9301 ttgctgacac tgtaccgcga ggccataggt caggcgcatg tggacgagca tactttccag
     9361 gagattacaa gtgtcagccg cgcgctgggg caggaggaca cgggcagcct ggaggcaacc
     9421 ctaaactacc tgctgaccaa ccggcggcag aagatcccct cgttgcacag tttaaacagc
     9481 gaggaggagc gcattttgcg ctacgtgcag cagagcgtga gccttaacct gatgcgcgac
     9541 ggggtaacgc ccagcgtggc gctggacatg accgcgcgca acatggaacc gggcatgtat
     9601 gcctcaaacc ggccgtttat caaccgccta atggactact tgcatcgcgc ggccgccgtg
     9661 aaccccgagt atttcaccaa tgccatcttg aacccgcact ggctaccgcc ccctggtttc
     9721 tacaccgggg gattcgaggt gcccgagggt aacgatggat tcctctggga cgacatagac
     9781 gacagcgtgt tttccccgca accgcagacc ctgctagagt tgcaacagcg cgagcaggca
     9841 gaggcggcgc tgcgaaagga aagcttccgc aggccaagca gcttgtccga tctaggcgct
     9901 gcggccccgc ggtcagatgc tagtagccca tttccaagct tgatagggtc tcttaccagc
     9961 actcgcacca cccgcccgcg cctgctgggc gaggaggagt acctaaacaa ctcgctgctg
    10021 cagccgcagc gcgaaaaaaa cctgcctccg gcatttccca acaacgggat agagagccta
    10081 gtggacaaga tgagtagatg gaagacgtac gcgcaggagc acagggacgt gccaggcccg
    10141 cgcccgccca cccgtcgtca aaggcacgac cgtcagcggg gtctggtgtg ggaggacgat
    10201 gactcggcag acgacagcag cgtcctggat ttgggaggga gtggcaaccc gtttgcgcac
    10261 cttcgcccca ggctggggag aatgttttaa aaaaaaaaaa gcatgatgca aaataaaaaa
    10321 ctcaccaagg ccatggcacc gagcgttggt tttcttgtat tccccttagt atgcggcgcg
    10381 cggcgatgta tgaggaaggt cctcctccct cctacgagag tgtggtgagc gcggcgccag
    10441 tggcggcggc gctgggttct cccttcgatg ctcccctgga cccgccgttt gtgcctccgc
    10501 ggtacctgcg gcctaccggg gggagaaaca gcatccgtta ctctgagttg gcacccctat
    10561 tcgacaccac ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca tccctgaact
    10621 accagaacga ccacagcaac tttctgacca cggtcattca aaacaatgac tacagcccgg
    10681 gggaggcaag cacacagacc atcaatcttg acgaccggtc gcactggggc ggcgacctga
    10741 aaaccatcct gcataccaac atgccaaatg tgaacgagtt catgtttacc aataagttta
    10801 aggcgcgggt gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag ctgaaatacg
    10861 agtgggtgga gttcacgctg cccgagggca actactccga gaccatgacc atagacctta
    10921 tgaacaacgc gatcgtggag cactacttga aagtgggcag acagaacggg gttctggaaa
    10981 gcgacatcgg ggtaaagttt gacacccgca acttcagact ggggtttgac cccgtcactg
    11041 gtcttgtcat gcctggggta tatacaaacg aagccttcca tccagacatc attttgctgc
    11101 caggatgcgg ggtggacttc acccacagcc gcctgagcaa cttgttgggc atccgcaagc
    11161 ggcaaccctt ccaggagggc tttaggatca cctacgatga tctggagggt ggtaacattc
    11221 ccgcactgtt ggatgtggac gcctaccagg cgagcttgaa agatgacacc gaacagggcg
    11281 ggggtggcgc aggcggcagc aacagcagtg gcagcggcgc ggaagagaac tccaacgcgg
    11341 cagccgcggc aatgcagccg gtggaggaca tgaacgatca tgccattcgc ggcgacacct
    11401 ttgccacacg ggctgaggag aagcgcgctg aggccgaagc agcggccgaa gctgccgccc
    11461 ccgctgcgca acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa cccctgacag
    11521 aggacagcaa gaaacgcagt tacaacctaa taagcaatga cagcaccttc acccagtacc
    11581 gcagctggta ccttgcatac aactacggcg accctcagac cggaatccgc tcatggaccc
    11641 tgctttgcac tcctgacgta acctgcggct cggagcaggt ctactggtcg ttgccagaca
    11701 tgatgcaaga ccccgtgacc ttccgctcca cgcgccagat cagcaacttt ccggtggtgg
    11761 gcgccgagct gttgcccgtg cactccaaga gcttctacaa cgaccaggcc gtctactccc
    11821 aactcatccg ccagtttacc tctctgaccc acgtgttcaa tcgctttccc gagaaccaga
    11881 ttttggcgcg cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt cctgctctca
    11941 cagatcacgg gacgctaccg ctgcgcaaca gcatcggagg agtccagcga gtgaccatta
    12001 ctgacgccag acgccgcacc tgcccctacg tttacaaggc cctgggcata gtctcgccgc
    12061 gcgtcctatc gagccgcact ttttgagcaa gcatgtccat ccttatatcg cccagcaata
    12121 acacaggctg gggcctgcgc ttcccaagca agatgtttgg cggggccaag aagcgctccg
    12181 accaacaccc agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg cacaaacgcg
    12241 gccgcactgg gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag gaggcgcgca
    12301 actacacgcc cacgccgcca ccagtgtcca cagtggacgc ggccattcag accgtggtgc
    12361 gcggagcccg gcgctatgct aaaatgaaga gacggcggag gcgcgtagca cgtcgccacc
    12421 gccgccgacc cggcactgcc gcccaacgcg cggcggcggc cctgcttaac cgcgcacgtc
    12481 gcaccggccg acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt attgtcactg
    12541 tgccccccag gtccaggcga cgagcggccg ccgcagcagc cgcggccatt agtgctatga
    12601 ctcagggtcg caggggcaac gtgtattggg tgcgcgactc ggttagcggc ctgcgcgtgc
    12661 ccgtgcgcac ccgccccccg cgcaactaga ttgcaagaaa aaactactta gactcgtact
    12721 gttgtatgta tccagcggcg gcggcgcgca acgaagctat gtccaagcgc aaaatcaaag
    12781 aagagatgct ccaggtcatc gcgccggaga tctatggccc cccgaagaag gaagagcagg
    12841 attacaagcc ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat gatgatgaac
    12901 ttgacgacga ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta cagtggaaag
    12961 gtcgacgcgt aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg cccggtgagc
    13021 gctccacccg cacctacaag cgcgtgtatg atgaggtgta cggcgacgag gacctgcttg
    13081 agcaggccaa cgagcgcctc ggggagtttg cctacggaaa gcggcataag gacatgctgg
    13141 cgttgccgct ggacgagggc aacccaacac ctagcctaaa gcccgtaaca ctgcagcagg
    13201 tgctgcccgc gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag tctggtgact
    13261 tggcacccac cgtgcagctg atggtaccca agcgccagcg actggaagat gtcttggaaa
    13321 aaatgaccgt ggaacctggg ctggagcccg aggtccgcgt gcggccaatc aagcaggtgg
    13381 cgccgggact gggcgtgcag accgtggacg ttcagatacc cactaccagt agcaccagta
    13441 ttgccaccgc cacagagggc atggagacac aaacgtcccc ggttgcctca gcggtggcgg
    13501 atgccgcggt gcaggcggtc gctgcggccg cgtccaagac ctctacggag gtgcaaacgg
    13561 acccgtggat gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg aagtacggcg
    13621 ccgccagcgc gctactgccc gaatatgccc tacatccttc cattgcgcct acccccggct
    13681 atcgtggcta cacctaccgc cccagaagac gagcaactac ccgacgccga accaccactg
    13741 gaacccgccg ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc gtgcgcaggg
    13801 tggctcgcga aggaggcagg accctggtgc tgccaacagc gcgctaccac cccagcatcg
    13861 tttaaaagcc ggtctttgtg gttcttgcag atatggccct cacctgccgc ctccgtttcc
    13921 cggtgccggg attccgagga agaatgcacc gtaggagggg catggccggc cacggcctga
    13981 cgggcggcat gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt cgcatgcgcg
    14041 gcggtatcct gcccctcctt attccactga tcgccgcggc gattggcgcc gtgcccggaa
    14101 ttgcatccgt ggccttgcag gcgcagagac actgattaaa aacaagttgc atgtggaaaa
    14161 atcaaaataa aaagtctgga ctctcacgct cgcttggtcc tgtaactatt ttgtagaatg
    14221 gaagacatca actttgcgtc tctggccccg cgacacggct cgcgcccgtt catgggaaac
    14281 tggcaagata tcggcaccag caatatgagc ggtggcgcct tcagctgggg ctcgctgtgg
    14341 agcggcatta aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc ctggaacagc
    14401 agcacaggcc agatgctgag ggataagttg aaagagcaaa atttccaaca aaaggtggta
    14461 gatggcctgg cctctggcat tagcggggtg gtggacctgg ccaaccaggc agtgcaaaat
    14521 aagattaaca gtaagcttga tccccgccct cccgtagagg agcctccacc ggccgtggag
    14581 acagtgtctc cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga agaaactctg
    14641 gtgacgcaaa tagacgagcc tccctcgtac gaggaggcac taaagcaagg cctgcccacc
    14701 acccgtccca tcgcgcccat ggctaccgga gtgctgggcc agcacacacc cgtaacgctg
    14761 gacctgcctc cccccgccga cacccagcag aaacctgtgc tgccaggccc gaccgccgtt
    14821 gttgtaaccc gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc gcgatcgttg
    14881 cggcccgtag ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg tctgggggtg
    14941 caatccctga agcgccgacg atgcttctga tagctaacgt gtcgtatgtg tgtcatgtat
    15001 gcgtccatgt cgccgccaga ggagctgctg agccgccgcg cgcccgcttt ccaagatggc
    15061 taccccttcg atgatgccgc agtggtctta catgcacatc tcgggccagg acgcctcgga
    15121 gtacctgagc cccgggctgg tgcagtttgc ccgcgccacc gagacgtact tcagcctgaa
    15181 taacaagttt agaaacccca cggtggcgcc tacgcacgac gtgaccacag accggtccca
    15241 gcgtttgacg ctgcggttca tccctgtgga ccgtgaggat actgcgtact cgtacaaggc
    15301 gcggttcacc ctagctgtgg gtgataaccg tgtgctggac atggcttcca cgtactttga
    15361 catccgcggc gtgctggaca ggggccctac ttttaagccc tactctggca ctgcctacaa
    15421 cgccctggct cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg ctactgctct
    15481 tgaaataaac ctagaagaag aggacgatga caacgaagac gaagtagacg agcaagctga
    15541 gcagcaaaaa actcacgtat ttgggcaggc gccttattct ggtataaata ttacaaagga
    15601 gggtattcaa ataggtgtcg aaggtcaaac acctaaatat gccgataaaa catttcaacc
    15661 tgaacctcaa ataggagaat ctcagtggta cgaaacagaa attaatcatg cagctgggag
    15721 agtcctaaaa aagactaccc caatgaaacc atgttacggt tcatatgcaa aacccacaaa
    15781 tgaaaatgga gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag aaagtcaagt
    15841 ggaaatgcaa tttttctcaa ctactgaggc agccgcaggc aatggtgata acttgactcc
    15901 taaagtggta ttgtacagtg aagatgtaga tatagaaacc ccagacactc atatttctta
    15961 catgcccact attaaggaag gtaactcacg agaactaatg ggccaacaat ctatgcccaa
    16021 caggcctaat tacattgctt ttagggacaa ttttattggt ctaatgtatt acaacagcac
    16081 gggtaatatg ggtgttctgg cgggccaagc atcgcagttg aatgctgttg tagatttgca
    16141 agacagaaac acagagcttt cataccagct tttgcttgat tccattggtg atagaaccag
    16201 gtacttttct atgtggaatc aggctgttga cagctatgat ccagatgtta gaattattga
    16261 aaatcatgga actgaagatg aacttccaaa ttactgcttt ccactgggag gtgtgattaa
    16321 tacagagact cttaccaagg taaaacctaa aacaggtcag gaaaatggat gggaaaaaga
    16381 tgctacagaa ttttcagata aaaatgaaat aagagttgga aataattttg ccatggaaat
    16441 caatctaaat gccaacctgt ggagaaattt cctgtactcc aacatagcgc tgtatttgcc
    16501 cgacaagcta aagtacagtc cttccaacgt aaaaatttct gataacccaa acacctacga
    16561 ctacatgaac aagcgagtgg tggctcccgg gctagtggac tgctacatta accttggagc
    16621 acgctggtcc cttgactata tggacaacgt caacccattt aaccaccacc gcaatgctgg
    16681 cctgcgctac cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc acatccaggt
    16741 gcctcagaag ttctttgcca ttaaaaacct ccttctcctg ccgggctcat acacctacga
    16801 gtggaacttc aggaaggatg ttaacatggt tctgcagagc tccctaggaa atgacctaag
    16861 ggttgacgga gccagcatta agtttgatag catttgcctt tacgccacct tcttccccat
    16921 ggcccacaac accgcctcca cgcttgaggc catgcttaga aacgacacca acgaccagtc
    16981 ctttaacgac tatctctccg ccgccaacat gctctaccct atacccgcca acgctaccaa
    17041 cgtgcccata tccatcccct cccgcaactg ggcggctttc cgcggctggg ccttcacgcg
    17101 ccttaagact aaggaaaccc catcactggg ctcgggctac gacccttatt acacctactc
    17161 tggctctata ccctacctag atggaacctt ttacctcaac cacaccttta agaaggtggc
    17221 cattaccttt gactcttctg tcagctggcc tggcaatgac cgcctgctta cccccaacga
    17281 gtttgaaatt aagcgctcag ttgacgggga gggttacaac gttgcccagt gtaacatgac
    17341 caaagactgg ttcctggtac aaatgctagc taactataac attggctacc agggcttcta
    17401 tatcccagag agctacaagg accgcatgta ctccttcttt agaaacttcc agcccatgag
    17461 ccgtcaggtg gtggatgata ctaaatacaa ggactaccaa caggtgggca tcctacacca
    17521 acacaacaac tctggatttg ttggctacct tgcccccacc atgcgcgaag gacaggccta
    17581 ccctgctaac ttcccctatc cgcttatagg caagaccgca gttgacagca ttacccagaa
    17641 aaagtttctt tgcgatcgca ccctttggcg catcccattc tccagtaact ttatgtccat
    17701 gggcgcactc acagacctgg gccaaaacct tctctacgcc aactccgccc acgcgctaga
    17761 catgactttt gaggtggatc ccatggacga gcccaccctt ctttatgttt tgtttgaagt
    17821 ctttgacgtg gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg tgtacctgcg
    17881 cacgcccttc tcggccggca acgccacaac ataaagaagc aagcaacatc aacaacagct
    17941 gccgccatgg gctccagtga gcaggaactg aaagccattg tcaaagatct tggttgtggg
    18001 ccatattttt tgggcaccta tgacaagcgc tttccaggct ttgtttctcc acacaagctc
    18061 gcctgcgcca tagtcaatac ggccggtcgc gagactgggg gcgtacactg gatggccttt
    18121 gcctggaacc cgcactcaaa aacatgctac ctctttgagc cctttggctt ttctgaccag
    18181 cgactcaagc aggtttacca gtttgagtac gagtcactcc tgcgccgtag cgccattgct
    18241 tcttcccccg accgctgtat aacgctggaa aagtccaccc aaagcgtaca ggggcccaac
    18301 tcggccgcct gtggactatt ctgctgcatg tttctccacg cctttgccaa ctggccccaa
    18361 actcccatgg atcacaaccc caccatgaac cttattaccg gggtacccaa ctccatgctc
    18421 aacagtcccc aggtacagcc caccctgcgt cgcaaccagg aacagctcta cagcttcctg
    18481 gagcgccact cgccctactt ccgcagccac agtgcgcaga ttaggagcgc cacttctttt
    18541 tgtcacttga aaaacatgta aaaataatgt actagagaca ctttcaataa aggcaaatgc
    18601 ttttatttgt acactctcgg gtgattattt acccccaccc ttgccgtctg cgccgtttaa
    18661 aaatcaaagg ggttctgccg cgcatcgcta tgcgccactg gcagggacac gttgcgatac
    18721 tggtgtttag tgctccactt aaactcaggc acaaccatcc gcggcagctc ggtgaagttt
    18781 tcactccaca ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc cgatatcttg
    18841 aagtcgcagt tggggcctcc gccctgcgcg cgcgagttgc gatacacagg gttgcagcac
    18901 tggaacacta tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc ggagatcaga
    18961 tccgcgtcca ggtcctccgc gttgctcagg gcgaacggag tcaactttgg tagctgcctt
    19021 cccaaaaagg gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg catcaaaagg
    19081 tgaccgtgcc cggtctgggc gttaggatac agcgcctgca taaaagcctt gatctgctta
    19141 aaagccacct gagcctttgc gccttcagag aagaacatgc cgcaagactt gccggaaaac
    19201 tgattggccg gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt ggagatctgc
    19261 accacatttc ggccccaccg gttcttcacg atcttggcct tgctagactg ctccttcagc
    19321 gcgcgctgcc cgttttcgct cgtcacatcc atttcaatca cgtgctcctt atttatcata
    19381 atgcttccgt gtagacactt aagctcgcct tcgatctcag cgcagcggtg cagccacaac
    19441 gcgcagcccg tgggctcgtg atgcttgtag gtcacctctg caaacgactg caggtacgcc
    19501 tgcaggaatc gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt cagctgcaac
    19561 ccgcggtgct cctcgttcag ccaggtcttg catacggccg ccagagcttc cacttggtca
    19621 ggcagtagtt tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc catcagcgcg
    19681 cgcgcagcct ccatgccctt ctcccacgca gacacgatcg gcacactcag cgggttcatc
    19741 accgtaattt cactttccgc ttcgctgggc tcttcctctt cctcttgcgt ccgcatacca
    19801 cgcgccactg ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc tttgccatgc
    19861 ttgattagca ccggtgggtt gctgaaaccc accatttgta gcgccacatc ttctctttct
    19921 tcctcgctgt ccacgattac ctctggtgat ggcgggcgct cgggcttggg agaagggcgc
    19981 ttctttttct tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg ccgcgggctg
    20041 ggtgtgcgcg gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga ctcgatacgc
    20101 cgcctcatcc gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga cggggacgac
    20161 acgtcctcca tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg ggtggtttcg
    20221 cgctgctcct cttcccgact ggccatttcc ttctcctata ggcagaaaaa gatcatggag
    20281 tcagtcgaga agaaggacag cctaaccgcc ccctctgagt tcgccaccac cgcctccacc
    20341 gatgccgcca acgcgcctac caccttcccc gtcgaggcac ccccgcttga ggaggaggaa
    20401 gtgattatcg agcaggaccc aggttttgta agcgaagacg acgaggaccg ctcagtacca
    20461 acagaggata aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca agtcgggcgg
    20521 ggggacgaaa ggcatggcga ctacctagat gtgggagacg acgtgctgtt gaagcatctg
    20581 cagcgccagt gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt gcccctcgcc
    20641 atagcggatg tcagccttgc ctacgaacgc cacctattct caccgcgcgt accccccaaa
    20701 cgccaagaaa acggcacatg cgagcccaac ccgcgcctca acttctaccc cgtatttgcc
    20761 gtgccagagg tgcttgccac ctatcacatc tttttccaaa actgcaagat acccctatcc
    20821 tgccgtgcca accgcagccg agcggacaag cagctggcct tgcggcaggg cgctgtcata
    20881 cctgatatcg cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg acgcgacgag
    20941 aagcgcgcgg caaacgctct gcaacaggaa aacagcgaaa atgaaagtca ctctggagtg
    21001 ttggtggaac tcgagggtga caacgcgcgc ctagccgtac taaaacgcag catcgaggtc
    21061 acccactttg cctacccggc acttaaccta ccccccaagg tcatgagcac agtcatgagt
    21121 gagctgatcg tgcgccgtgc gcagcccctg gagagggatg caaatttgca agaacaaaca
    21181 gaggagggcc tacccgcagt tggcgacgag cagctagcgc gctggcttca aacgcgcgag
    21241 cctgccgact tggaggagcg acgcaaacta atgatggccg cagtgctcgt taccgtggag
    21301 cttgagtgca tgcagcggtt ctttgctgac ccggagatgc agcgcaagct agaggaaaca
    21361 ttgcactaca cctttcgaca gggctacgta cgccaggcct gcaagatctc caacgtggag
    21421 ctctgcaacc tggtctccta ccttggaatt ttgcacgaaa accgccttgg gcaaaacgtg
    21481 cttcattcca cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg cgtttactta
    21541 tttctatgct acacctggca gacggccatg ggcgtttggc agcagtgctt ggaggagtgc
    21601 aacctcaagg agctgcagaa actgctaaag caaaacttga aggacctatg gacggccttc
    21661 aacgagcgct ccgtggccgc gcacctggcg gacatcattt tccccgaacg cctgcttaaa
    21721 accctgcaac agggtctgcc agacttcacc agtcaaagca tgttgcagaa ctttaggaac
    21781 tttatcctag agcgctcagg aatcttgccc gccacctgct gtgcacttcc tagcgacttt
    21841 gtgcccatta agtaccgcga atgccctccg ccgctttggg gccactgcta ccttctgcag
    21901 ctagccaact accttgccta ccactctgac ataatggaag acgtgagcgg tgacggtcta
    21961 ctggagtgtc actgtcgctg caacctatgc accccgcacc gctccctggt ttgcaattcg
    22021 cagctgctta acgaaagtca aattatcggt acctttgagc tgcagggtcc ctcgcctgac
    22081 gaaaagtccg cggctccggg gttgaaactc actccggggc tgtggacgtc ggcttacctt
    22141 cgcaaatttg tacctgagga ctaccacgcc cacgagatta ggttctacga agaccaatcc
    22201 cgcccgccta atgcggagct taccgcctgc gtcattaccc agggccacat tcttggccaa
    22261 ttgcaagcca tcaacaaagc ccgccaagag tttctgctac gaaagggacg gggggtttac
    22321 ttggaccccc agtccggcga ggagctcaac ccaatccccc cgccgccgca gccctatcag
    22381 cagcagccgc gggcccttgc ttcccaggat ggcacccaaa aagaagctgc agctgccgcc
    22441 gccacccacg gacgaggagg aatactggga cagtcaggca gaggaggttt tggacgagga
    22501 ggaggaggac atgatggaag actgggagag cctagacgag gaagcttccg aggtcgaaga
    22561 ggtgtcagac gaaacaccgt caccctcggt cgcattcccc tcgccggcgc cccagaaatc
    22621 ggcaaccggt tccagcatgg ctacaacctc cgctcctcag gcgccgccgg cactgcccgt
    22681 tcgccgaccc aaccgtagat gggacaccac tggaaccagg gccggtaagt ccaagcagcc
    22741 gccgccgtta gcccaagagc aacaacagcg ccaaggctac cgctcatggc gcgggcacaa
    22801 gaacgccata gttgcttgct tgcaagactg tgggggcaac atctccttcg cccgccgctt
    22861 tcttctctac catcacggcg tggccttccc ccgtaacatc ctgcattact accgtcatct
    22921 ctacagccca tactgcaccg gcggcagcgg cagcaacagc agcggccaca cagaagcaaa
    22981 ggcgaccgga tagcaagact ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag
    23041 gaggaggagc gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag cttagaaaca
    23101 ggatttttcc cactctgtat gctatatttc aacagagcag gggccaagaa caagagctga
    23161 aaataaaaaa caggtctctg cgatccctca cccgcagctg cctgtatcac aaaagcgaag
    23221 atcagcttcg gcgcacgctg gaagacgcgg aggctctctt cagtaaatac tgcgcgctga
    23281 ctcttaagga ctagtttcgc gccctttctc aaatttaagc gcgaaaacta cgtcatctcc
    23341 agcggccaca cccggcgcca gcacctgttg tcagcgccat tatgagcaag gaaattccca
    23401 cgccctacat gtggagttac cagccacaaa tgggacttgc ggctggagct gcccaagact
    23461 actcaacccg aataaactac atgagcgcgg gaccccacat gatatcccgg gtcaacggaa
    23521 tacgcgccca ccgaaaccga attctcctgg aacaggcggc tattaccacc acacctcgta
    23581 ataaccttaa tccccgtagt tggcccgctg ccctggtgta ccaggaaagt cccgctccca
    23641 ccactgtggt acttcccaga gacgcccagg ccgaagttca gatgactaac tcaggggcgc
    23701 agcttgcggg cggctttcgt cacagggtgc ggtcgcccgg gcagggtata actcacctga
    23761 caatcagagg gcgaggtatt cagctcaacg acgagtcggt gagctcctcg cttggtctcc
    23821 gtccggacgg gacatttcag atcggcggcg ccggccgctc ttcattcacg cctcgtcagg
    23881 caatcctaac tctgcagacc tcgtcctctg agccgcgctc tggaggcatt ggaactctgc
    23941 aatttattga ggagtttgtg ccatcggtct actttaaccc cttctcggga cctcccggcc
    24001 actatccgga tcaatttatt cctaactttg acgcggtaaa ggactcggcg gacggctacg
    24061 actgaatgtt aagtggagag gcagagcaac tgcgcctgaa acacctggtc cactgtcgcc
    24121 gccacaagtg ctttgcccgc gactccggtg agttttgcta ctttgaattg cccgaggatc
    24181 atatcgaggg cccggcgcac ggcgtccggc ttaccgccca gggagagctt gcccgtagcc
    24241 tgattcggga gtttacccag cgccccctgc tagttgagcg ggacagggga ccctgtgttc
    24301 tcactgtgat ttgcaactgt cctaaccctg gattacatca agatcctcta gttaatgtca
    24361 ggtcgcctaa gtcgattaac tagagtaccc ggggatctta ttccctttaa ctaataaaaa
    24421 aaaataataa agcatcactt acttaaaatc agttagcaaa tttctgtcca gtttattcag
    24481 cagcacctcc ttgccctcct cccagctctg gtattgcagc ttcctcctgg ctgcaaactt
    24541 tctccacaat ctaaatggaa tgtcagtttc ctcctgttcc tgtccatccg cacccactat
    24601 cttcatgttg ttgcagatga agcgcgcaag accgtctgaa gataccttca accccgtgta
    24661 tccatatgac acggaaaccg gtcctccaac tgtgcctttt cttactcctc cctttgtatc
    24721 ccccaatggg tttcaagaga gtccccctgg ggtactctct ttgcgcctat ccgaacctct
    24781 agttacctcc aatggcatgc ttgcgctcaa aatgggcaac ggcctctctc tggacgaggc
    24841 cggcaacctt acctcccaaa atgtaaccac tgtgagccca cctctcaaaa aaaccaagtc
    24901 aaacataaac ctggaaatat ctgcacccct cacagttacc tcagaagccc taactgtggc
    24961 tgccgccgca cctctaatgg tcgcgggcaa cacactcacc atgcaatcac aggccccgct
    25021 aaccgtgcac gactccaaac ttagcattgc cacccaagga cccctcacag tgtcagaagg
    25081 aaagctagcc ctgcaaacat caggccccct caccaccacc gatagcagta cccttactat
    25141 cactgcctca ccccctctaa ctactgccac tggtagcttg ggcattgact tgaaagagcc
    25201 catttataca caaaatggaa aactaggact aaagtacggg gctcctttgc atgtaacaga
    25261 cgacctaaac actttgaccg tagcaactgg tccaggtgtg actattaata atacttcctt
    25321 gcaaactaaa gttactggag ccttgggttt tgattcacaa ggcaatatgc aacttaatgt
    25381 agcaggagga ctaaggattg attctcaaaa cagacgcctt atacttgatg ttagttatcc
    25441 gtttgatgct caaaaccaac taaatctaag actaggacag ggccctcttt ttataaactc
    25501 agcccacaac ttggatatta actacaacaa aggcctttac ttgtttacag cttcaaacaa
    25561 ttccaaaaag cttgaggtta acctaagcac tgccaagggg ttgatgtttg acgctacagc
    25621 catagccatt aatgcaggag atgggcttga atttggttca cctaatgcac caaacacaaa
    25681 tcccctcaaa acaaaaattg gccatggcct agaatttgat tcaaacaagg ctatggttcc
    25741 taaactagga actggcctta gttttgacag cacaggtgcc attacagtag gaaacaaaaa
    25801 taatgataag ctaactttgt ggaccacacc agctccatct cctaactgta gactaaatgc
    25861 agagaaagat gctaaactca ctttggtctt aacaaaatgt ggcagtcaaa tacttgctac
    25921 agtttcagtt ttggctgtta aaggcagttt ggctccaata tctggaacag ttcaaagtgc
    25981 tcatcttatt ataagatttg acgaaaatgg agtgctacta aacaattcct tcctggaccc
    26041 agaatattgg aactttagaa atggagatct tactgaaggc acagcctata caaacgctgt
    26101 tggatttatg cctaacctat cagcttatcc aaaatctcac ggtaaaactg ccaaaagtaa
    26161 cattgtcagt caagtttact taaacggaga caaaactaaa cctgtaacac taaccattac
    26221 actaaacggt acacaggaaa caggagacac aactccaagt gcatactcta tgtcattttc
    26281 atgggactgg tctggccaca actacattaa tgaaatattt gccacatcct cttacacttt
    26341 ttcatacatt gcccaagaat aaagaatcgt ttgtgttatg tttcaacgtg cctaggcaaa
    26401 atagcaccct cccgctccag aacaacatac agcgcttcca cagcggcagc cataacagtc
    26461 agccttacca gtaaaaaaga aaacctatta aaaaaacacc actcgacacg gcaccagctc
    26521 aatcagtcac agtgtaaaaa agggccaagt gcagagcgag tatatatagg actaaaaaat
    26581 gacgtaacgg ttaaagtcca caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag
    26641 aaacgaaagc caaaaaaccc acaacttcct caaatcgtca cttccgtttt cccacgttac
    26701 gtcacttccc attttaagaa aactacaatt cccaacacat acaagttact ccgccctaaa
    26761 acctacgtca cccgccccgt tcccacgccc cgcgccacgt cacaaactcc accccctcat
    26821 tatcatattg gcttcaatcc aaaataaggt atattattga tgatgttaat taacatgcat
    26881 ggatcctacg tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
    26941 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
    27001 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
    27061 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
    27121 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
    27181 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
    27241 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
    27301 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
    27361 cctaacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
    27421 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
    27481 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
    27541 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
    27601 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
    27661 cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg
    27721 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
    27781 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
    27841 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
    27901 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
    27961 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
    28021 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
    28081 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct
    28141 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
    28201 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
    28261 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
    28321 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    28381 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    28441 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
    28501 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    28561 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
    28621 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    28681 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    28741 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    28801 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    28861 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    28921 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    28981 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    29041 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    29101 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    29161 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    29221 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    29281 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    29341 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    29401 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    29461 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    29521 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    29581 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    29641 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    29701 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    29761 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    29821 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
    29881 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    29941 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    30001 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    30061 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    30121 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
    30181 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    30241 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    30301 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    30361 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    30421 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    30481 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgtctag ctacgaattc
    30541 ttcgacagct tcgaaactag aatcgatttc gaaactagct taagggtggg aaagaatata
    30601 taaggtgggg gtcttatgta gttttgtatc tgttttgcag cagccgccgc cgccatgagc
    30661 accaactcgt ttgatggaag cattgtgagc tcatatttga caacgcgcat gcccccatgg
    30721 gccggggtgc gtcagaatgt gatgggctcc agcattgatg gtcgccccgt cctgcccgca
    30781 aactctacta ccttgaccta cgagaccgtg tctggaacgc cgttggaga
//

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