pLZ414

LOCUS       pLZ414            8970 bp DNA     circular SYN 10-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=oMHS-CMV-F
     misc_feature    829
                     /label=putative transcriptional start
     promoter        863..882
                     /label=T7 promoter
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_recomb     905..938
                     /label=LoxP
     regulatory      1001..1010
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1007..1069
                     /label=Igk secretion signal
     CDS             1075
                     /codon_start=1
                     /label=linker
                     /translation=""
     CDS             1076..1774
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    1781..2524
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     misc_feature    2054..2056
                     /label=F93A
                     /note="F93A"
     misc_feature    2508..2524
                     /label=EpoR-D1_D2_TM
     misc_feature    2534..3362
                     /label=IL6ST
     misc_feature    2561..2587
                     /label=box1
     misc_feature    2885..2887
                     /label=Y759A
     polyA_signal    3391..3615
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      3624..3808
                     /label=SV40 late polyA
     protein_bind    complement(3845..3878)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3891..3959
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
     misc_feature    3981..4490
                     /label=NLuc (nano Luciferase)
                     /label=nonstandard type:  reporter
     misc_feature    4011..4013
                     /label=cyt-STOP (TGG->TGA)
     terminator      4497..4519
                     /label=SV40 late polyA
     terminator      4564..4791
                     /label=bGH polyA
     polyA_signal    4567..4791
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4837..5265
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     primer_bind     complement(4851..4875)
                     /label=184-WKG-599-R
     primer_bind     5136..5155
                     /label=189-WKG-612-F
     promoter        5279..5608
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    5511..5560
                     /label=P_SV40 TSS-region
     CDS             5675..6466
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     polyA_signal    6643..6764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6809..6829)
                     /label=M13-rev
     primer_bind     complement(6813..6829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(6835..6857)
                     /label=LacO
     protein_bind    6837..6853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6861..6891)
                     /label=lac promoter
     protein_bind    6906..6927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7033..7973
                     /label=ColE1
                     /note="ColE1"
     misc_feature    complement(7977..8834)
                     /label=AmpR
                     /label=nonstandard type: resistance
     promoter        complement(8835..8939)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gcttataact tcgtatagca tacattatac gaagttatcg gcgcgccgcg gccgcgatca
      961 attcggtacc gaagttccta ttccgaattc ctagtggccg gccaccatgg agaccgatac
     1021 cttattactg tgggtgcttc ttctgtgggt tcccggtagc accggagacg gatccgacat
     1081 cgagaagctg tgcaagaagg cggaagagga ggccaaggag gcccaggaga aggccgacga
     1141 gctgagacaa cgacaccccg attcgcaggc ggcggaagac gcggaggatc tcgcaaatct
     1201 ggctgtcgcc gccgtgctga ccgcttgtct gctggcccag gagcatccca acgccgatat
     1261 cgctaagctg tgcatcaagg ccgcgagcga agctgcggag gccgcgtcaa aagccgccga
     1321 gctggcccaa cggcacccag acagtcaagc agccagagat gctatcaaac tcgcctctca
     1381 agccgcccgc gccgtgatcc tggccatcat gctcgccgca gagaatccca acgctgatat
     1441 tgcaaagcta tgtattaagg cggccagcga agccgcagag gctgcctcga aagctgcgga
     1501 acttgcacag cggcaccccg actcccaggc cgcccgagat gcaattaaac tggccagtca
     1561 ggccgctgag gcggtggaga gagccatctg gctggccgcc gagaacccca atgccgatat
     1621 cgccaaaaaa tgcatcaaag cggcctccga agcagccgag gaggcctcta aagctgccga
     1681 agaggctcaa agacacccag acagccagaa ggcacgcgac gagatcaagg aggcctctca
     1741 gaaggccgag gaggtgaagg agagatgcaa gagcgaattc gcaccttcac ccagcctccc
     1801 ggaccccaag tttgagagca aagcggccct gctggcatcc cggggctccg aagaacttct
     1861 gtgcttcacc caacgcttgg aagacttggt gtgtttctgg gaggaagcgg cgagctccgg
     1921 gatggacttc aactacagct tctcatacca gctcgagggt gagtcacgaa agtcatgtag
     1981 cctgcaccag gctcccaccg tccgcggctc cgtgcgtttc tggtgttcac tgccaacagc
     2041 ggacacatcg agtgctgtgc cgctggagct gcaggtgacg gaggcgtccg gttctcctcg
     2101 ctatcaccgc atcatccata tcaatgaagt agtgctcctg gacgcccccg cggggctgct
     2161 ggcgcgccgg gcagaagagg gcagccacgt ggtgctgcgc tggctgccac ctcctggagc
     2221 acctatgacc acccacatcc gatatgaagt ggacgtgtcg gcaggcaacc gggcaggagg
     2281 gacacaaagg gtggaggtcc tggaaggccg cactgagtgt gttctgagca acctgcgggg
     2341 cgggacgcgc tacaccttcg ctgttcgagc gcgcatggcc gagccgagct tcagcggatt
     2401 ctggagtgcc tggtctgagc ccgcgtcact actgaccgct agcgacctgg accctctcat
     2461 cttgacgctg tctctcattc tggtcctcat ctcgttgttg ctgacggttc tggccctgct
     2521 gtccgcagca gcaaataagc gagacctaat taaaaaacac atctggccta atgttccaga
     2581 tccttcaaag agtcatattg cccagtggtc acctcacact cctccaaggc acaattttaa
     2641 ttcaaaagat caaatgtatt cagatggcaa tttcactgat gtaagtgttg tggaaataga
     2701 agcaaatgac aaaaagcctt ttccagaaga tctgaaatca ttggacctgt tcaaaaagga
     2761 aaaaattaat actgaaggac acagcagtgg tattgggggg tcttcatgca tgtcatcttc
     2821 taggccaagc atttctagca gtgatgaaaa tgaatcttca caaaacactt cgagcactgt
     2881 ccaggcttct accgtggtac acagtggcta cagacaccaa gttccgtcag tccaagtctt
     2941 ctcaagatcc gagtctaccc agcccttgtt agattcagag gagcggccag aagatctaca
     3001 attagtagat catgtagatg gcggtgatgg tatttttccc aggcaacagt acttcaaaca
     3061 gaactgcagt cagcatgaat ccagtccaga tatttcacat tttgaaaggt caaagcaagt
     3121 ttcatcagtc aatgaggaag attttgttag acttaaacag cagatttcag atcatatttc
     3181 acaatcctgt ggatctgggc aaatgaaaat gtttcaggaa gtttctgcag cagatgcttt
     3241 tggtccaggt actgagggac aagtagaaag atttgaaaca gttggcatgg aggctgcgac
     3301 tgatgaaggc atgcctaaaa gttacttacc acagactgta cggcaaggcg gctacatgcc
     3361 tcagtaacct gcagggctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3421 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3481 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3541 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     3601 cggtgggctc tatggcttct gaggataaga tacattgatg agtttggaca aaccacaact
     3661 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3721 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3781 gttcaggggg aggtgtggga ggttttttaa agcgcttggt accgtctttc cagtggttaa
     3841 ttaaataact tcgtatagca tacattatac gaagttatct cgaggccacc atgactagtg
     3901 agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgacg
     3961 ctagtggtgg ttctggtatg gtcttcacac tcgaagattt cgttggggac tggcgacaga
     4021 cagccggcta caacctggac caagtccttg aacagggagg tgtgtccagt ttgtttcaga
     4081 atctcggggt gtccgtaact ccgatccaaa ggattgtcct gagcggtgaa aatgggctga
     4141 agatcgacat ccatgtcatc atcccgtatg aaggtctgag cggcgaccaa atgggccaga
     4201 tcgaaaaaat ttttaaggtg gtgtaccctg tggatgatca tcactttaag gtgatcctgc
     4261 actatggcac actggtaatc gacggggtta cgccgaacat gatcgactat ttcggacggc
     4321 cgtatgaagg catcgccgtg ttcgacggca aaaagatcac tgtaacaggg accctgtgga
     4381 acggcaacaa aattatcgac gagcgcctga tcaaccccga cggctccctg ctgttccgag
     4441 taaccatcaa cggagtgacc ggctggcggc tgtgcgaacg cattctggcg atcgatagac
     4501 atgataagat acattgatgt ctagaagctt ctagagggcc cgtttaaacc cgctgatcag
     4561 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4621 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4681 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4741 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     4801 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     4861 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4921 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4981 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5041 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5101 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5161 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5221 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     5281 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5341 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5401 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5461 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5521 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     5581 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     5641 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     5701 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5761 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5821 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     5881 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5941 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6001 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6061 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6121 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6181 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6241 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6301 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6361 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6421 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6481 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6541 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6601 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6661 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6721 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6781 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6841 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6901 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6961 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7021 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7081 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7141 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7201 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7261 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7321 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7381 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7441 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7501 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7561 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7621 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7681 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7741 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7801 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7861 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7921 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7981 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8041 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8101 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8161 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8221 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8281 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8341 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8401 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8461 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8521 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8581 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8641 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8701 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8761 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8821 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8881 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8941 acatttcccc gaaaagtgcc acctgacgtc
//