| 质粒名称: | pETCON-IgG1/design_50 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 30301 |
| 存储实验室: | David Baker |
|
载体骨架基本信息 |
|
| 载体名称: | pETCON |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | IgG Fc Designed Binder IgG1 |
| 物种来源: | Designed Sequence |
| 插入大小: | 507 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NdeI(未破坏) |
| 3' 克隆位点: | XhoI(未破坏) |
| 5' 测序引物 | UP-GS |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pETCON-IgG1/design_50 was a gift from David Baker (Addgene plasmid # 30301 ; http://n2t.net/addgene:30301 ; RRID:Addgene_30301) |
| 参考文献部分: | Community-Wide Assessment of Protein-Interface Modeling Suggests Improvements to Design Methodology. Fleishman SJ, Whitehead TA, Strauch EM, Corn JE, Qin S, Zhou HX, Mitchell JC, Demerdash ON, Takeda-Shitaka M, Terashi G, Moal IH, Li X, Bates PA, Zacharias M, Park H, Ko JS, Lee H, Seok C, Bourquard T, Bernauer J, Poupon A, Aze J, Soner S, Ovali SK, Ozbek P, Tal NB, Haliloglu T, Hwang H, Vreven T, Pierce BG, Weng Z, Perez-Cano L, Pons C, Fernandez-Recio J, Jiang F, Yang F, Gong X, Cao L, Xu X, Liu B, Wang P, Li C, Wang C, Robert CH, Guharoy M, Liu S, Huang Y, Li L, Guo D, Chen Y, Xiao Y, London N, Itzhaki Z, Schueler-Furman O, Inbar Y, Patapov V, Cohen M, Schreiber G, Tsuchiya Y, Kanamori E, Standley DM, Nakamura H, Kinoshita K, Driggers CM, Hall RG, Morgan JL, Hsu VL, Zhan J, Yang Y, Zhou Y, Kastritis PL, Bonvin AM, Zhang W, Camacho CJ, Kilambi KP, Sircar A, Gray JJ, Ohue M, Uchikoga N, Matsuzaki Y, Ishida T, Akiyama Y, Khashan R, Bush S, Fouches D, Tropsha A, Esquivel-Rodriguez J, Kihara D, Stranges PB, Jacak R, Kuhlman B, Huang SY, Zou X, Wodak SJ, Janin J, Baker D. J Mol Biol. 2011 Sep 29. 10.1016/j.jmb.2011.09.031 PubMed 22001016 |
买家导航
pETCON-IgG1/design_50载体质粒基本信息
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pETCON-IgG1/design_50质粒载体简介
pETCON-IgG1/design_50质粒序列载体序列
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pETCON-IgG1/design_50质粒载体应用举例
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