pEF6/myc-His A

价格：2500元

联系方式：I47-825O-882O

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pEF6/myc-HisA载体基本信息

出品公司:	Invitrigen
载体名称:	pEF6/myc-His A
质粒类型:	哺乳细胞表达载体
高拷贝/低拷贝:	高拷贝
启动子:	EF-1α
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5798 bp
5' 测序引物及序列:	T7 Forward：TAATACGACTCACTATAGGG
3' 测序引物及序列:	--
载体标签:	His Tag (6x), c-Myc Epitope Tag
载体抗性:	氨苄青霉素（Ampicillin）
筛选标记:	Blasticidin
备注:	易于克隆到其它载体中去；高表达水平。
产品目录号:	V962-20
稳定性:	瞬表达
组成型:	组成型表达
病毒/非病毒:	非病毒

pEF6/myc-HisA载体图谱质粒图谱和多克隆位点信息

pEF6-Myc-His A载体图谱

pEF6/myc-HisA载体简介

pEF6/myc-HisA载体序列

LOCUS       pEF6/myc-His A	5798 bp 	DNA	SYN
DEFINITION  pEF6/myc-His A
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="pEF6/myc-His A"
                     /mol_type="other DNA"
     promoter        358..1529
                     /label="EF1a_promoter"
     CDS             complement(649..1269)
                     /label="ORF frame 3"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     misc_feature    1477..1497
                     /label="EF1a_fwd_primer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     promoter        1546..1564
                     /label="T7_promoter"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     misc_feature    complement(1765..1782)
                     /label="BGH_rev_primer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     terminator      1768..1995
                     /label="bGH_PA_terminator"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     rep_origin      2058..2364
                     /label="f1_origin"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     misc_feature    complement(2478..2498)
                     /label="pBABE_3_primer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     misc_feature    complement(2484..2699)
                     /label="SV40_enhancer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     promoter        2496..2764
                     /label="SV40_promoter"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     rep_origin      2663..2740
                     /label="SV40_origin"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     misc_feature    2725..2744
                     /label="SV40pro_F_primer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     promoter        2858..2925
                     /label="EM7_promoter"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     gene            2926..3324
                     /label="blast"
                     /gene="blast"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     terminator      3485..3604
                     /label="SV40_PA_terminator"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     misc_feature    3573..3592
                     /label="EBV_rev_primer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     promoter        complement(3648..3666)
                     /label="M13_reverse_primer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     misc_feature    complement(3665..3687)
                     /label="M13_pUC_rev_primer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     promoter        complement(3701..3730)
                     /label="lac_promoter"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     gene            complement(4802..5662)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"
     CDS             complement(4802..5662)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5704..5732)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
   61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG
  121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC
  181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT
  241 GATTATTGAC TAGGCTTTTG CAAAAAGCTT TGCAAAGATG GATAAAGTTT TAAACAGAGA
  301 GGAATCTTTG CAGCTAATGG ACCTTCTAGG TCTTGAAAGG AGTGGGAATT GGCTCCGGTG
  361 CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG
  421 GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT
  481 ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG
  541 TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT
  601 CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACCT
  661 GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA
  721 GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT
  781 GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT
  841 CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC
  901 TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG
  961 ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC
 1021 CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC
 1081 CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA GTTGCGTGAG
 1141 CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG ACGCGGCGCT
 1201 CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG
 1261 TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA
 1321 GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC
 1381 ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG
 1441 AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA
 1501 GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTAGCTT GGTACTAATA CGACTCACTA
 1561 TAGGGAGACC CAAGCTGGCT AGGTAAGCTT GGTACCGAGC TCGGATCCAC TAGTCCAGTG
 1621 TGGTGGAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT CGAGTCTAGA GGGCCCTTCG
 1681 AACAAAAACT CATCTCAGAA GAGGATCTGA ATATGCATAC CGGTCATCAT CACCATCACC
 1741 ATTGAGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG
 1801 TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT
 1861 AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG
 1921 GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG
 1981 CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC
 2041 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG
 2101 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA
 2161 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA
 2221 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC
 2281 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG
 2341 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT
 2401 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA
 2461 ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC
 2521 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC
 2581 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT
 2641 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC
 2701 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA
 2761 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC
 2821 GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA ATCATCGGCA
 2881 TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC CAAGCCTTTG
 2941 TCTCAAGAAG AATCCACCCT CATTGAAAGA GCAACGGCTA CAATCAACAG CATCCCCATC
 3001 TCTGAAGACT ACAGCGTCGC CAGCGCAGCT CTCTCTAGCG ACGGCCGCAT CTTCACTGGT
 3061 GTCAATGTAT ATCATTTTAC TGGGGGACCT TGTGCAGAAC TCGTGGTGCT GGGCACTGCT
 3121 GCTGCTGCGG CAGCTGGCAA CCTGACTTGT ATCGTCGCGA TCGGAAATGA GAACAGGGGC
 3181 ATCTTGAGCC CCTGCGGACG GTGCCGACAG GTGCTTCTCG ATCTGCATCC TGGGATCAAA
 3241 GCCATAGTGA AGGACAGTGA TGGACAGCCG ACGGCAGTTG GGATTCGTGA ATTGCTGCCC
 3301 TCTGGTTATG TGTGGGAGGG CTAAGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT
 3361 ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG
 3421 GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC
 3481 CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
 3541 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
 3601 TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT
 3661 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT
 3721 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC
 3781 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
 3841 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
 3901 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
 3961 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGGAACCGTA
 4021 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
 4081 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC
 4141 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT
 4201 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
 4261 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG
 4321 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
 4381 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA
 4441 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT
 4501 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
 4561 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA
 4621 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
 4681 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT
 4741 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA
 4801 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA
 4861 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC
 4921 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA
 4981 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC
 5041 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA
 5101 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT
 5161 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG
 5221 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC
 5281 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT
 5341 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT
 5401 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC
 5461 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT
 5521 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA
 5581 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA
 5641 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG
 5701 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG
 5761 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC
//

pVW5JI	pTALETF_v2 (NG)
IR2	pMSCG19
pNIT-3	pGFP22
pLVX-Myc-MCS-Strep-IRES-Puro	pAc5.1-V5-His A
pMIB/V5-His A	p3xFLAG-KV2.1
pSW510	pLenti CMV/TO Neo empty (w215-1)
pDR540	pEF1/His B
pBHR68	pGL4.77[hRlucP/Hygro]
pWW60-1	pEF4/His B
pSC101 and pKG2	pLVX-rHom-Sec1
pGL4.83[hRlucP/Puro]	pAX01-ComK
pTCK303	pIB/V5-His
pQE-32	pCas
pKLAC2	pHC79-2cos/tk
pHIC-PI	phCMV1
pFastBac Dual	pTrcHis2 C
pFastBac-GST-C	pET-12c(+)
pOS1	pET302/NT-His
pCMV5	pYES2-EGFP
pFastBac1	pUCDM
pME12::Tn5-259	pTYB2
pB-gyrase_TA	pcDNA4/V5-His B
pAc5.1a	pDEST10
pCTAP-Shuttle-A	pKO11
pCambia1201	pPH3
pcDNA4/HisMax A	pBad/Myc-His A
pBT-5	pcDNA3.1+P2A-eGFP
pBApo-CMV-Pur	pSilencer5.1-H1 Retro
DR2	pMYs-IRES-GFP
pJW342	pCDF1-MCS2-EF1-Puro
pGL101	pIAβ8
pCR4-35	pcDNA6.2/V5-PL-DEST
pFB-hrGFP	pZeoSV2 (+)
pE	pFA1
pET-32 EK/LIC	pYES2-kan
pMMB33	pECFP-Nuc
pcDNA3.1+/N-GST(TEV)	pCMV-3Tag-3a
pFtsH_TA	pSRE-Luc
pTT5	pIJ702-87del(78-86, 88-132)
p53-Luc	pCold-GST
pFN31K Nluc-CMV-neo-Flexi	pGL4.39[luc2P/ATF6 RE/Hygro]
pCB1535	pXIS11
pET-28a-EGFP	pSinRep5
pDP5rs	pRS426
VaD1	pPZP122
pcDNA3.1/His B	pLKO-DEST-EGFP
pGL4.25[luc2CP/minP]	pMKO.1-GFP
pCAMBIA3200	PX391
pCas-Guide-EF1a-GFP	pacAd5 9.2-100
pMV261	pG5luc

pmGFP	pHAGE2-TetOminiCMV-SKM
pCMV-2NLS-Cas9-6XMS2	pET21a-BirA
pGL4-ERSE2-luc2P-Hygro	SIRT3 Flag
PG13-luc (wt p53 binding sites)	jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcode
pHyo094 (eMutaT7)	MSP2135
mTert-pBabe-puro	pTNT-B18R-6His
pSELECT-HA-mFOXO1	pcDNA3.1 myc-NL-GW
CaV1.2	pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
Stat3 Flag pRc/CMV	pHIV-iRFP720-E2A-Luc
EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (F	pEcgRNA
pFE-sfGFP	pCMV-ABE7.9
pHAGE2-TetOminiCMV-mCherry	pcDNA3/Flag-METTL3
pGIPZ-PD-L1-EGFP	pLV-puro-N-3HA-SREBP1-C-Flag
pTK299	pCMVR8.74
mTagBFP2-TOMM20-N-10	p415-GalL-Cas9-CYC1t
pBAD18-Cm	pBS-hBAF60a
pminiTol2	pCMVInt
pEGFP-NMHC II-C	sfGFP-Cx43K258stop
pY001 (pFnCpf1_full)	pJQ200mp18
hCas9_D10A	pDA077
pNI3	TrkC-GFP
pHAGE NFkB-TA-LUC-UBC-GFP-W	pCRISPR-aBEST
pGL3 2 kb prom. CD274	pCAG Cas9-2A-Citrine
pRK GFP Paxillin	Flag-TRIM24
pCMV-HsPeg10rc3	BECLIN-HA WT
pGL3-mArg1 promoter/enhancer -31/-3810	pHAtC
DNMT3b KO Hyg	pCMV-SynA
pRK5-p18-HA	LentiGuide Cherry
pR26 GFP Dest	pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T
pEJS654 All-in-One AAV-sgRNA-hNmeCas9	pBECKP-km
pYLCRISPR/Cas9pUbi-N	pRN11
pAAV-EF1a-DIO-mCherry	pGM1202
pBabe-puro-miR-10b sponge	MSP680
pc DNA FRT TO- APPSwed/Ind	pMDIAI
xCas9(3.6)-BE3	pSBbi-GN
pMXs-hc-MYC	pHIV-Luc-ZsGreen
pET15b_Bst-LF	FUGW-PercevalHR
pCI neo-hEST2	pNLF1W
Plasmid L5 attB::Pleft* mCherry	pAAV-EF1a-DIO-HTB
pCas9	pCMVHA hEZH2
pLentipuro3/TO/V5-GW/EGFP-Firefly Lucife	pBabe-hygro PyMT
pBFC1207	pPBbsr2-4031NES
BCR/ABL P190 transgenic construct	Tcf/Lef:H2B-GFP
pUCmini-iCAP-AAV.CAP-B22	pSIN4-EF1a-LMO2-IRES-Puro
pPH37	pRJPaph-bjGFP
pC0061 PsmCas13 (B05) His6-TwinStrep-SUM	pCAS9-TPC
pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-V	pmScarlet_Giantin_C1
pLenti PGK Puro DEST (w529-2)	pcDNA3.1-mCherry
pF151 pcDNA3.1(+)SOD1WT	pMaster1
cytoplasmic EKAR (Cerulean-Venus)	pMSCV-FlagMLL-pl-ENL(5613)

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