| 质粒名称: | pEB3-mfRFP-N1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000328 |
| 产品用途: | Expresses EB30-mfRFP in mammalian cells culture |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | N1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: |
EB3, mfRFP
|
| 物种来源: | Human, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A Set of Novel Far-Red Fluorescent Proteins for Temporal Domain Multiplexing and Super-Resolution Imaging
Olumayowa F., Rong Z., Wang R., Papadaki S., Wang X., Cao J., ubach F.V., Koster R.W., Namikawa K., Piatkevich K.D.
bioRxiv 2025.06.01.657196 (2025)
|
pEB3-mfRFP-N1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pEB3-mfRFP-N1载体质粒基本信息
pEB3-mfRFP-N1质粒图谱载体图谱和pEB3-mfRFP-N1载体序列质粒序列多克隆位点信息
pEB3-mfRFP-N1质粒载体简介
pEB3-mfRFP-N1质粒序列载体序列
LOCUS pEB3-mfRFP-N1 5587 bp DNA circular SYN 22-NOV-2023
DEFINITION In vivo visualization of microtuble plus ends (can be used for
colocalization studies).
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5587)
AUTHORS Chertkova AO, Mastop M, Postma M, van Bommel N, van der Niet S,
Batenburg KL, Joosen L, Gadella TWJ, Okada Y, Goedhart J
TITLE Robust and Bright Genetically Encoded Fluorescent Markers for
Highlighting Structures and Compartments in Mammalian Cells
JOURNAL bioRxiv 160374
REFERENCE 2 (bases 1 to 5587)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv
160374"
FEATURES Location/Qualifiers
source 1..5587
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 646..655
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 652..1494
/codon_start=1
/label=EB3
/translation="MAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYC
QFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDN
FEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSP
TGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHETDAQILELNQQLVDLKLTVDGLEKE
RDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY
"
primer_bind 1483..1533
/label=0908-EB3-F
regulatory 1510..1515
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1516..2247
/codon_start=1
/label=mfRFP
/translation="MVSKGEELIKENMPLKLYMEGTVNNHHFKCTAEGEGKPYEGTQTQ
RIKVVEGGPLPFAFDILATSFMYGSHTFIDHTPGIPDFWKQSFPEGFTWERVTTYEDGG
VLIATQDTSLHDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEAHTELLYPTDGGLEGRTA
LALKLVGGGHLLCNFKTIYRSKKPAKNLKMPGVYYVDYRLERIEEADNETYVEQHEVAV
ARYCDLPTKLGHKLKGMDELYK"
primer_bind complement(2226..2265)
/label=0908-EB3-R
polyA_signal 2375..2496
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2412..2431)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2466..2485
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2503..2958)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2640..2661)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2852..2871
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2985..3089
/gene="bla"
/label=AmpR promoter
promoter 3091..3448
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3114..3134)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3299..3434
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3361..3380
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3384..3395)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3483..4277
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3537..3556)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4147..4166
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4465..4484)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4509..4556
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4885..5473
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5374..5393
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgactgccac catggccgtc
661 aatgtgtact ccacatctgt gaccagtgaa aatctgagtc gccatgatat gcttgcatgg
721 gtcaacgact ccctgcacct caactatacc aagatagaac agctttgttc aggggcagcc
781 tactgccagt tcatggacat gctcttcccc ggctgtgtgc acttgaggaa agtgaagttc
841 caggccaaac tagagcatga atacatccac aacttcaagg tgctgcaagc agctttcaag
901 aagatgggtg ttgacaaaat cattcctgta gagaaattag tgaaaggaaa attccaagat
961 aattttgagt ttattcagtg gtttaagaaa ttctttgacg caaactatga tggaaaggat
1021 tacaaccctc tgctggcgcg gcagggccag gacgtagcgc cacctcctaa cccaggtgat
1081 cagatcttca acaaatccaa gaaactcatt ggcacagcag ttccacagag gacgtccccc
1141 acaggcccaa aaaacatgca gacctctggc cggctgagca atgtggcccc cccctgcatt
1201 ctccggaaga atcctccatc agcccgaaat ggcggccatg agactgatgc ccaaattctt
1261 gaactcaacc aacagctggt ggacttgaag ctgacagtgg atgggctgga gaaggaacgt
1321 gacttctact tcagcaaact tcgtgacatc gagctcatct gccaggagca tgaaagtgaa
1381 aacagccctg ttatctcagg catcattggc atcctctatg ccacagagga aggattcgca
1441 ccccctgagg acgatgagat tgaagagcat caacaagaag accaggacga gtacctggat
1501 ccaccggtcg ccaccatggt gagcaagggc gaggagctga ttaaggagaa catgccactg
1561 aagctgtaca tggagggcac cgttaacaat caccacttca agtgcacagc tgagggcgaa
1621 ggcaagccct acgagggcac ccagacccag agaatcaagg tggtcgaggg cggccctctc
1681 cccttcgcct tcgacatcct ggctaccagt ttcatgtacg gcagtcacac cttcatcgac
1741 cacaccccgg gcatccccga cttctggaag cagtccttcc ccgagggctt cacgtgggag
1801 agagtcacca catacgaaga cgggggcgtc ctgatcgcga cccaggacac cagcctccat
1861 gacggctgcc tcatctacaa cgtcaagttg agaggggtga acttcccatc caacggccct
1921 gtgatgcaaa agaaaacact cggctgggag gcacacaccg agcttttgta ccccactgac
1981 ggcggtctgg aaggcagaac cgcgctggcc ctgaagcttg tgggcggggg ccacctattg
2041 tgtaacttca agactatata cagatccaag aaacccgcta agaacctcaa gatgcccggc
2101 gtctactatg tggactacag actggaaaga atcgaggagg ccgacaatga gacctacgtc
2161 gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctaccaaact ggggcacaag
2221 cttaagggca tggacgagct gtacaagtaa gaattggccg cgactctaga tcataatcag
2281 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
2341 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
2401 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
2461 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
2521 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
2581 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
2641 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
2701 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
2761 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
2821 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
2881 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
2941 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
3001 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
3061 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
3121 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
3181 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
3241 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
3301 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
3361 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
3421 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
3481 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
3541 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
3601 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
3661 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
3721 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
3781 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
3841 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
3901 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
3961 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
4021 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
4081 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
4141 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
4201 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
4261 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
4321 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
4381 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
4441 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
4501 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
4561 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
4621 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
4681 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
4741 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
4801 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
4861 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
4921 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
4981 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
5041 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
5101 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
5161 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
5221 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
5281 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
5341 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
5401 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
5461 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
5521 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
5581 catgcat
//
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