| 质粒名称: | pLXSN16E6E7 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 121441 |
| 存储实验室: | Denise Galloway |
|
载体骨架基本信息 |
|
| 载体名称: | pLXSN |
| 空载体大小: | 5874 bp |
| 完整质粒大小: | 6700bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | Retroviral |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HPV16 E6E7 |
| 插入大小: | 770 bp |
| GenBank编号 | NP_041325.1 NP_041326.1 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI(未破坏) |
| 3’克隆位点: | BamHI (未破坏) |
| 5’测序引物 | pLXSNF |
| 3’测序引物 | pLXSNR |
|
文献引用方法 |
|
| 材料和方法部分: | pLXSN16E6E7 was a gift from Denise Galloway (Addgene plasmid # 52394 ; http://n2t.net/addgene:52394 ; RRID:Addgene_52394) |
| 参考文献部分: | The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. Halbert CL, Demers GW, Galloway DA. J Virol. 1991 Jan;65(1):473-8. PubMed 1845902 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLXSN16E6E7 6883 bp ds-DNA circular SYN 06-SEP-2022
DEFINITION The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant
retrovirus providing efficient gene transfers and expression of the
HPV16 E6 and E7 genes..
ACCESSION .
VERSION .
KEYWORDS pLXSN16E6E7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6883)
AUTHORS Halbert CL, Demers GW, Galloway DA
TITLE The E7 gene of human papillomavirus type 16 is sufficient for
immortalization of human epithelial cells.
JOURNAL J Virol. 1991 Jan;65(1):473-8.
PUBMED 1845902
REFERENCE 2 (bases 1 to 6883)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 6, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6883
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 588..763
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 826..1025
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1226..1642
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1572..1594
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1610..1626
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(2512..2532)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2517..2846
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2697..2832
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2759..2778
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2906..3700
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2960..2979)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3570..3589
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
LTR 3929..4522
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(4625..4647)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 4733..4873
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 4788..4807
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4888..4905)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5059..5647)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5139..5158)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5818..6678)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6441..6460
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6679..6783)
/gene="bla"
/label=AmpR promoter
primer_bind 6851..6869
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
1621 cctcactcct tctctaggcg ccggaattcg agctcggtac ccccggttag tataaaagca
1681 gacattttat gcaccaaaag agaactgcaa tgtttcagga cccacaggag cgacccagaa
1741 agttaccaca gttatgcaca gagctgcaaa caactataca tgatataata ttagaatgtg
1801 tgtactgcaa gcaacagtta ctgcgacgtg aggtatatga ctttgctttt cgggatttat
1861 gcatagtata tagagatggg aatccatatg ctgtatgtga taaatgttta aagttttatt
1921 ctaaaattag tgagtataga cattattgtt atagtttgta tggaacaaca ttagaacagc
1981 aatacaacaa accgttgtgt gatttgttaa ttaggtgtat taactgtcaa aagccactgt
2041 gtcctgaaga aaagcaaaga catctggaca aaaagcaaag attccataat ataaggggtc
2101 ggtggaccgg tcgatgtatg tcttgttgca gatcatcaag aacacgtaga gaaacccagc
2161 tgtaatcatg catggagata cacctacatt gcatgaatat atgttagatt tgcaaccaga
2221 gacaactgat ctctactgtt atgagcaatt aaatgacagc tcagaggagg aggatgaaat
2281 agatggtcca gctggacaag cagaaccgga cagagcccat tacaatattg taaccttttg
2341 ttgcaagtgt gactctacgc ttcggttgtg cgtacaaagc acacacgtag acattcgtac
2401 tttggaagac ctgttaatgg gcacactagg aattgtgtgc cccatctgtt ctcagaaacc
2461 ataatctacc atggctgatc ctgcaggcat gcaagctggg gatccggctg tggaatgtgt
2521 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
2581 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
2641 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
2701 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
2761 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
2821 tttttggagg cctaggcttt tgcaaaaagc ttgggctgca ggtcgaggcg gatctgatca
2881 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
2941 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
3001 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
3061 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
3121 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
3181 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
3241 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
3301 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
3361 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
3421 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
3481 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
3541 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
3601 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
3661 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
3721 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
3781 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
3841 ggggatctca tgctggagtt cttcgcccac cccaacacga gccatagata aaataaaaga
3901 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
3961 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt
4021 tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
4081 taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa
4141 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
4201 agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
4261 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
4321 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg
4381 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
4441 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
4501 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
4561 accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg
4621 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
4681 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
4741 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
4801 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
4861 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
4921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
4981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
5041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
5101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
5161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
5221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
5281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
5341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
5401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
5461 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
5521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
5581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
5641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
5701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
5761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
5821 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
5881 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
5941 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
6001 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
6061 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
6121 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
6181 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
6241 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
6301 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
6361 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
6421 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
6481 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
6541 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
6601 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
6661 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
6721 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
6781 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
6841 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caa
//
