LOCUS pCDH-EF1-TfR20-SNAP-IRES-Puro 10545 bp ds-DNA circular SYN 13-JUL-2021
DEFINITION Lentiviral expression of a transferrin receptor-SNAP-tag fusion in
mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCDH-EF1-TfR20-SNAP-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10545)
AUTHORS FitzGerald LI, Aurelio L, Chen M, Yuen D, Rennick JJ, Graham B,
Johnston APR
TITLE A molecular sensor to quantify the localization of proteins, DNA and
nanoparticles in cells.
JOURNAL Nat Commun. 2020 Sep 8;11(1):4482. doi: 10.1038/s41467-020-18082-8.
PUBMED 32901011
REFERENCE 2 (bases 1 to 10545)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10545
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 6..232
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 233..413
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 460..585
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1078..1311
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1496..1540
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1807..1923
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 1966..2177
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 2190..2458
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
regulatory 4855..4864
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4861..5406
/codon_start=1
/product="human O6-alkylguanine-DNA-alkyltransferase
variant that forms covalent bonds with benzylguanine
derivatives"
/label=SNAP-tag(R)
/note="fast-reacting variant, optimized for mammalian
expression"
/translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
VKEWLLAHEGHRLGKPGLG"
misc_feature 5419..6005
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(5586..5603)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 5813..5832
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 6018..6617
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6018..6037)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6514..6534
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 6624..7212
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6677..6697)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7095..7106)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7286..7519
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7591..7712
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7628..7647)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7682..7701
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 7752..7887
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7814..7833
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(7920..7936)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7920..7936)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7933..7955)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7944..7960
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7968..7998)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8013..8034
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8151..8168)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8322..8910)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8402..8421)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9081..9941)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9704..9723
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9942..10046)
/gene="bla"
/label=AmpR promoter
primer_bind 10114..10132
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(10170..10192)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 10292..10311
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10505..10527
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 10519..10536
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 10520..10536
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
1921 tttatcgata ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
1981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
2041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
2101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
2161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
2221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
2281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
2341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
2401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
2461 agctgtgacc ggcgcctact ctagagctag cgctaccgga ctcagatctc gagcgccacc
2521 atgatggatc aagctagatc agcattctct aacttgtttg gtggagaacc attgtcatat
2581 acccggttca gcctggctcg gcaagtagat ggcgataaca gtcatgtgga gatgaaactt
2641 gctgtagatg aagaagaaaa tgctgacaat aacacaaagg ccaatgtcac aaaaccaaaa
2701 aggtgtagtg gaagtatctg ctatgggact attgctgtga tcgtcttttt cttgattgga
2761 tttatgattg gctacttggg ctattgtaaa ggggtagaac caaaaactga gtgtgagaga
2821 ctggcaggaa ccgagtctcc agtgagggag gagccaggag aggacttccc tgcagcacgt
2881 cgcttatatt gggatgacct gaagagaaag ttgtcggaga aactggacag cacagacttc
2941 accagcacca tcaagctgct gaatgaaaat tcatatgtcc ctcgtgaggc tggatctcaa
3001 aaagatgaaa atcttgcgtt gtatgttgaa aatcaatttc gtgaatttaa actcagcaaa
3061 gtctggcgtg atcaacattt tgttaagatt caggtcaaag acagcgctca aaactcggtg
3121 atcatagttg ataagaacgg tagacttgtt tacctggtgg agaatcctgg gggttatgtg
3181 gcgtatagta aggctgcaac agttactggt aaactggtcc atgctaattt tggtactaaa
3241 aaagattttg aggatttata cactcctgtg aatggatcta tagtgattgt cagagcaggg
3301 aaaatcacct ttgcagaaaa ggttgcaaat gctgaaagct taaatgcaat tggtgtgttg
3361 atatacatgg accagactaa atttcccatt gttaacgcag aactttcatt ctttggacat
3421 gctcatctgg ggacaggtga cccttacaca cctggattcc cttccttcaa ccacactcag
3481 tttccaccat ctcggtcatc aggattgcct aatatacctg tccagacaat ctccagagct
3541 gctgcagaaa agctgtttgg gaatatggaa ggagactgtc cctctgactg gaaaacagac
3601 tctacatgta ggatggtaac ctcagaaagc aagaatgtga agctcactgt gagcaatgtg
3661 ctgaaagaga taaaaattct taacatcttt ggagttatta aaggctttgt agaaccagat
3721 cactatgttg tagttggggc ccagagagat gcatggggcc ctggagctgc aaaatccggt
3781 gtaggcacag ctctcctatt gaaacttgcc cagatgttct cagatatggt cttaaaagat
3841 gggtttcagc ccagcagaag cattatcttt gccagttgga gtgctggaga ctttggatcg
3901 gttggtgcca ctgaatggct agagggatac ctttcgtccc tgcatttaaa ggctttcact
3961 tatattaatc tggataaagc ggttcttggt accagcaact tcaaggtttc tgccagccca
4021 ctgttgtata cgcttattga gaaaacaatg caaaatgtga agcatccggt tactgggcaa
4081 tttctatatc aggacagcaa ctgggccagc aaagttgaga aactcacttt agacaatgct
4141 gctttccctt tccttgcata ttctggaatc ccagcagttt ctttctgttt ttgcgaggac
4201 acagattatc cttatttggg taccaccatg gacacctata aggaactgat tgagaggatt
4261 cctgagttga acaaagtggc acgagcagct gcagaggtcg ctggtcagtt cgtgattaaa
4321 ctaacccatg atgttgaatt gaacctggac tatgagaggt acaacagcca actgctttca
4381 tttgtgaggg atctgaacca atacagagca gacataaagg aaatgggcct gagtttacag
4441 tggctgtatt ctgctcgtgg agacttcttc cgtgctactt ccagactaac aacagatttc
4501 gggaatgctg agaaaacaga cagatttgtc atgaagaaac tcaatgatcg tgtcatgaga
4561 gtggagtatc acttcctctc tccctacgta tctccaaaag agtctccttt ccgacatgtc
4621 ttctggggct ccggctctca cacgctgcca gctttactgg agaacttgaa actgcgtaaa
4681 caaaataacg gtgcttttaa tgaaacgctg ttcagaaacc agttggctct agctacttgg
4741 actattcagg gagctgcaaa tgccctctct ggtgacgttt gggacattga caatgagttt
4801 tcagaattcg gctccaccgg ctccaccggc tccaccggcg cggatccacc ggtcgccacc
4861 atggacaaag actgcgaaat gaagcgcacc accctggata gccctctggg caagctggaa
4921 ctgtctgggt gcgaacaggg cctgcaccgt atcatcttcc tgggcaaagg aacatctgcc
4981 gccgacgccg tggaagtgcc tgccccagcc gccgtgctgg gcggaccaga gccactgatg
5041 caggccaccg cctggctcaa cgcctacttt caccagcctg aggccatcga ggagttccct
5101 gtgccagccc tgcaccaccc agtgttccag caggagagct ttacccgcca ggtgctgtgg
5161 aaactgctga aagtggtgaa gttcggagag gtcatcagct acagccacct ggccgccctg
5221 gccggcaatc ccgccgccac cgccgccgtg aaaaccgccc tgagcggaaa tcccgtgccc
5281 attctgatcc cctgccaccg ggtggtgcag ggcgacctgg acgtgggggg ctacgagggc
5341 gggctcgccg tgaaagagtg gctgctggcc cacgagggcc acagactggg caagcctggg
5401 ctgggttaag cggccgcgcc cctctccctc ccccccccct aacgttactg gccgaagccg
5461 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
5521 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
5581 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
5641 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
5701 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
5761 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
5821 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
5881 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
5941 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
6001 caaccatggc gtccggaatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
6061 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
6121 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
6181 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
6241 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
6301 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
6361 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
6421 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
6481 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
6541 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
6601 gcaagcccgg tgcctaggtc gacaatcaac ctctggatta caaaatttgt gaaagattga
6661 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
6721 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
6781 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
6841 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
6901 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
6961 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
7021 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
7081 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
7141 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
7201 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
7261 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat
7321 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
7381 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
7441 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
7501 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
7561 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
7621 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
7681 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
7741 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
7801 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
7861 agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt cgtaatcatg
7921 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
7981 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
8041 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
8101 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
8161 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
8221 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
8281 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
8341 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
8401 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
8461 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
8521 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
8581 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
8641 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
8701 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
8761 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
8821 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
8881 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
8941 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
9001 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
9061 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
9121 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
9181 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
9241 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
9301 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
9361 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
9421 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
9481 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
9541 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
9601 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
9661 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
9721 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
9781 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
9841 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
9901 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
9961 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
10021 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
10081 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
10141 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
10201 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
10261 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
10321 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
10381 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
10441 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
10501 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
