| 载体名称: | pCMV3-C-His |
|---|---|
| 质粒类型: | 哺乳动物细胞载体；逆病毒包装载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6.1 kb |
| 5’测序引物: | T7(TAATACGACTCACTATAGGG) |
| 3’测序引物: | BGH(TAGAAGGCACAGTCGAGG) |
| 载体标签: | C-His(CACCATCACCACCATCATCACCACCATCAC) |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 潮霉素 |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 备注: |
• CMV 启动子: 碱基 250-837
• 增强子: 碱基 838-1385
• SV40 早期启动子: 碱基 2345-2714
• 潮霉素 ORF: 碱基 2732-3757
• pUC 原点: 碱基 4400-5073
• 卡那霉素 ORF: 碱基 5147-5962
|
买家导航
pCMV3-CHis载体基本信息
pCMV3-CHis载体图谱质粒图谱和多克隆位点信息
pCMV3-CHis载体简介
pCMV3-CHis载体序列
LOCUS pCMV3-C-His 6104 bp DNA circular SYN 01-FEB-2024
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6104)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Feb 1, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|1047945600|
COMMENT VNTDBDATE|1047945600|
COMMENT VNTNAME|pCMV3-C-His|
COMMENT VNTAUTHORNAME|Ming|
FEATURES Location/Qualifiers
enhancer 253..632
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 633..836
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1386..1404
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1550..1579
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
polyA_signal 1642..1866
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1912..2340
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2354..2683
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2534..2669
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2732..3757
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
polyA_signal 3887..4008
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4057..4073)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4081..4097
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4105..4135)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4150..4171
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4459..5047)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5147..5962)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg agtacattta tattggctca tgtccaatat
241 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
301 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
361 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
421 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
481 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
541 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
601 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
661 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
721 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
781 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
841 tagtgaaccg tcagatcctc actctcttcc gcatcgctgt ctgcgagggc cagctgttgg
901 gctcgcggtt gaggacaaac tcttcgcggt ctttccagta ctcttggatc ggaaacccgt
961 cggcctccga acggtactcc gccaccgagg gacctgagcg agtccgcatc gaccggatcg
1021 gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc aaggtaggct gagcaccgtg
1081 gcgggcggca gcgggtggcg gtcggggttg tttctggcgg aggtgctgct gatgatgtaa
1141 ttaaagtagg cggtcttgag acggcggatg gtcgaggtga ggtgtggcag gcttgagatc
1201 cagctgttgg ggtgagtact ccctctcaaa agcgggcatt acttctgcgc taagattgtc
1261 agtttccaaa aacgaggagg atttgatatt cacctggccc gatctggcca tacacttgag
1321 tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg tccaagttta
1381 aactttaata cgactcacta taggggccgc caccaagctt ggtaccgcta gcggatccgt
1441 taaccttaag accggtatgg gctggtcctg catcatcctg ttcctcgtgg cgaccgcgac
1501 cggggtccac agcgatatca tcgatagcgc tcccgggggt ggaggctctc accatcacca
1561 ccatcatcac caccatcact aaactcgagt ctagagcggc cgccgaattc gggcccgttt
1621 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
1681 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
1741 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
1801 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
1861 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
1921 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
1981 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgcag
2041 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
2101 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
2161 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
2221 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
2281 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
2341 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
2401 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
2461 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
2521 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
2581 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
2641 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctct cgggagcttg
2701 tatatccatt ttcggatctg atcagcacgt gatgaaaaag cctgaactca ccgcgacgtc
2761 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
2821 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
2881 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
2941 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
3001 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
3061 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
3121 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
3181 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
3241 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
3301 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
3361 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
3421 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
3481 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
3541 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
3601 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
3661 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
3721 ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtgctacgag atttcgattc
3781 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ctggctggat
3841 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
3901 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
3961 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
4021 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
4081 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
4141 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
4201 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
4261 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
4321 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
4381 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
4441 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
4501 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
4561 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
4621 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
4681 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
4741 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
4801 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
4861 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
4921 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
4981 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
5041 atctcaagaa gatcctttga tcttttctac ggggtctgag cgcggaaccc ctatttgttt
5101 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
5161 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
5221 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
5281 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
5341 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
5401 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
5461 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
5521 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
5581 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
5641 ggaatgctgt tttcccaggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
5701 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
5761 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
5821 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
5881 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
5941 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
6001 ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
6061 gtagaaatcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
