pET-28b-Cas9-His

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pET-28b-Cas9-His
质粒类型: CRISPR/Cas9载体;细菌基因敲除载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: lac/T7
载体大小: 9375 bp
5' 测序引物及序列: T7 forward:5’TAATACGACTCACTATAGGG 3’
3' 测序引物及序列: --
载体标签: His tag (C-ter)
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
克隆菌株: DH5alpha
宿主细胞(系): 细菌,如大肠杆菌
备注: --
产品目录号: #47327
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pET-28b-Cas9-His载体图谱



载体简介

载体序列

LOCUS       pET-28b-Cas9-His Sequencing Result	9375 bp 	DNA	SYN
DEFINITION  pET-28b-Cas9-His Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="pET-28b-Cas9-His Sequencing Result"
                     /mol_type="other DNA"
     misc_feature    230..252
                     /label="pGEX_3_primer"
     misc_feature    complement(246..437)
                     /label="ROP"
     gene            complement(909..1184)
                     /label="tet (611 - 336)"
                     /gene="tet (611 - 336)"
     misc_feature    complement(1246..2337)
                     /label="lacI"
     CDS             complement(1246..2205)
                     /label="ORF frame 1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(2078..2578)
                     /label="ORF frame 3"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     gene            complement(2429..2680)
                     /label="tet (887 - 636)"
                     /gene="tet (887 - 636)"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     misc_feature    2612..2631
                     /label="pBRrevBam_primer"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     promoter        2715..2733
                     /label="T7_promoter"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     misc_feature    2733..2760
                     /label="lacO"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     misc_feature    2778..2794
                     /label="T7_transl_en_RBS"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     CDS             complement(2784..3281)
                     /label="ORF frame 2"
                     /translation="MRQREIDQPQVSLISTVYKLLPQMIYGWVLFMVRHLVHDIAKDW
                     VALVLFILLHQKGLLQPMEERVIHLSHLITKDLLQVADPILSAGISAPCCSFEPRGFG
                     RLPGVEQEGANEVLLYAVAIGIAQNFEFFARHLVLVRNDGPADAVCADIEPNGVLLVH
                     GISPS*"
     CDS             2802..6974
                     /label="ORF frame 3"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSRADPKKKRK
                     VAAALEHHHHHH*"
     CDS             complement(4206..4724)
                     /label="ORF frame 2"
                     /translation="MSVSFQAFFNHLPIFKQRKGEDNVLKNVLVLLIVQEVLVFNDFQ
                     EIVIRSQGCVEAILHSADFNRVETFNLFEIVFFELFHGNFPVRLEEEVHDSFLLLSRQ
                     ECWLSHPFCDVFDLGELVINCEVLVQQRVFRKHLFVRQIFIKVSHPFDEGLGRGPLIH
                     DFLEVPGSDGLF*"
     CDS             complement(4788..5321)
                     /label="ORF frame 2"
                     /translation="MIHVVVGEPIDVQFLIHVHVPAVLQVVQVELLILKLGVFNWVFL
                     KDLGPQFFYTLFNPLHPFPTVLLSLLGSLVLSGHLDNDILGLMPSHYFDEFIHDLNGL
                     QYSLFDSWATCKISDVLVKTVPLARNLCFLDVLLKGERVIMDQLHEVPVGKSIGLKKI
                     QDCLSTLLVSDPIDQFS*"
     CDS             complement(6315..6848)
                     /label="ORF frame 2"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    6918..6938
                     /label="SV40 NLS"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    6924..6938
                     /label="NLS"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    6954..6971
                     /label="6xHIS"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     terminator      6982..7110
                     /label="T7_terminator"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    complement(7024..7042)
                     /label="T7_Terminal_primer"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     gene            complement(7669..8484)
                     /label="KanR2"
                     /gene="KanR2"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     CDS             complement(7669..8484)
                     /label="ORF frame 1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     rep_origin      8590..9209
                     /label="pBR322_origin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
ORIGIN
    1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgCAATGGTG
   61 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG
  121 CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA
  181 CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC
  241 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA
  301 TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG
  361 AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT
  421 TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG
  481 ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG
  541 TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA
  601 ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC
  661 CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT
  721 TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC
  781 GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA
  841 GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC
  901 CGTGGGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA
  961 CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG
 1021 ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC
 1081 ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG
 1141 CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGAGAT
 1201 CCCGGTGCCT AATGAGTGAG CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
 1261 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
 1321 GGTTTGCGTA TTGGGCGCCA GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG
 1381 ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC
 1441 CAGCAGGCGA AAATCCTGTT TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC
 1501 GGTATCGTCG TATCCCACTA CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT
 1561 GGCGCGCATT GCGCCCAGCG CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT
 1621 GCCCTCATTC AGCATTTGCA TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC
 1681 CCGTTCCGCT ATCGGCTGAA TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG
 1741 CAGACGCGCC GAGACAGAAC TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA
 1801 TGCGACCAGA TGCTCCACGC CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT
 1861 GATGGGTGTC TGGTCAGAGA CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC
 1921 CACAGCAATG GCATCCTGGT CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG
 1981 CGCGAGAAGA TTGTGCACCG CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA
 2041 CACCACCACG CTGGCACCCA GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA
 2101 CGGCGCGTGC AGGGCCAGAC TGGAGGTGGC AACGCCAATC AGCAACGACT GTTTGCCCGC
 2161 CAGTTGTTGT GCCACGCGGT TGGGAATGTA ATTCAGCTCC GCCATCGCCG CTTCCACTTT
 2221 TTCCCGCGTT TTCGCAGAAA CGTGGCTGGC CTGGTTCACC ACGCGGGAAA CGGTCTGATA
 2281 AGAGACACCG GCATACTCTG CGACATCGTA TAACGTTACT GGTTTCACAT TCACCACCCT
 2341 GAATTGACTC TCTTCCGGGC GCTATCATGC CATACCGCGA AAGGTTTTGC GCCATTCGAT
 2401 GGTGTCCGGG ATCTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA
 2461 GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC
 2521 CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA
 2581 GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA
 2641 CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT
 2701 CGATCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC
 2761 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC CATGGACAAG AAGTACTCCA
 2821 TTGGGCTCGA TATCGGCACA AACAGCGTCG GCTGGGCCGT CATTACGGAC GAGTACAAGG
 2881 TGCCGAGCAA AAAATTCAAA GTTCTGGGCA ATACCGATCG CCACAGCATA AAGAAGAACC
 2941 TCATTGGCGC CCTCCTGTTC GACTCCGGGG AGACGGCCGA AGCCACGCGG CTCAAAAGAA
 3001 CAGCACGGCG CAGATATACC CGCAGAAAGA ATCGGATCTG CTACCTGCAG GAGATCTTTA
 3061 GTAATGAGAT GGCTAAGGTG GATGACTCTT TCTTCCATAG GCTGGAGGAG TCCTTTTTGG
 3121 TGGAGGAGGA TAAAAAGCAC GAGCGCCACC CAATCTTTGG CAATATCGTG GACGAGGTGG
 3181 CGTACCATGA AAAGTACCCA ACCATATATC ATCTGAGGAA GAAGCTTGTA GACAGTACTG
 3241 ATAAGGCTGA CTTGCGGTTG ATCTATCTCG CGCTGGCGCA TATGATCAAA TTTCGGGGAC
 3301 ACTTCCTCAT CGAGGGGGAC CTGAACCCAG ACAACAGCGA TGTCGACAAA CTCTTTATCC
 3361 AACTGGTTCA GACTTACAAT CAGCTTTTCG AAGAGAACCC GATCAACGCA TCCGGAGTTG
 3421 ACGCCAAAGC AATCCTGAGC GCTAGGCTGT CCAAATCCCG GCGGCTCGAA AACCTCATCG
 3481 CACAGCTCCC TGGGGAGAAG AAGAACGGCC TGTTTGGTAA TCTTATCGCC CTGTCACTCG
 3541 GGCTGACCCC CAACTTTAAA TCTAACTTCG ACCTGGCCGA AGATGCCAAG CTTCAACTGA
 3601 GCAAAGACAC CTACGATGAT GATCTCGACA ATCTGCTGGC CCAGATCGGC GACCAGTACG
 3661 CAGACCTTTT TTTGGCGGCA AAGAACCTGT CAGACGCCAT TCTGCTGAGT GATATTCTGC
 3721 GAGTGAACAC GGAGATCACC AAAGCTCCGC TGAGCGCTAG TATGATCAAG CGCTATGATG
 3781 AGCACCACCA AGACTTGACT TTGCTGAAGG CCCTTGTCAG ACAGCAACTG CCTGAGAAGT
 3841 ACAAGGAAAT TTTCTTCGAT CAGTCTAAAA ATGGCTACGC CGGATACATT GACGGCGGAG
 3901 CAAGCCAGGA GGAATTTTAC AAATTTATTA AGCCCATCTT GGAAAAAATG GACGGCACCG
 3961 AGGAGCTGCT GGTAAAGCTT AACAGAGAAG ATCTGTTGCG CAAACAGCGC ACTTTCGACA
 4021 ATGGAAGCAT CCCCCACCAG ATTCACCTGG GCGAACTGCA CGCTATCCTC AGGCGGCAAG
 4081 AGGATTTCTA CCCCTTTTTG AAAGATAACA GGGAAAAGAT TGAGAAAATC CTCACATTTC
 4141 GGATACCCTA CTATGTAGGC CCCCTCGCCC GGGGAAATTC CAGATTCGCG TGGATGACTC
 4201 GCAAATCAGA AGAGACCATC ACTCCCTGGA ACTTCGAGGA AGTCGTGGAT AAGGGGGCCT
 4261 CTGCCCAGTC CTTCATCGAA AGGATGACTA ACTTTGATAA AAATCTGCCT AACGAAAAGG
 4321 TGCTTCCTAA ACACTCTCTG CTGTACGAGT ACTTCACAGT TTATAACGAG CTCACCAAGG
 4381 TCAAATACGT CACAGAAGGG ATGAGAAAGC CAGCATTCCT GTCTGGAGAG CAGAAGAAAG
 4441 CTATCGTGGA CCTCCTCTTC AAGACGAACC GGAAAGTTAC CGTGAAACAG CTCAAAGAAG
 4501 ACTATTTCAA AAAGATTGAA TGTTTCGACT CTGTTGAAAT CAGCGGAGTG GAGGATCGCT
 4561 TCAACGCATC CCTGGGAACG TATCACGATC TCCTGAAAAT CATTAAAGAC AAGGACTTCC
 4621 TGGACAATGA GGAGAACGAG GACATTCTTG AGGACATTGT CCTCACCCTT ACGTTGTTTG
 4681 AAGATAGGGA GATGATTGAA GAACGCTTGA AAACTTACGC TCATCTCTTC GACGACAAAG
 4741 TCATGAAACA GCTCAAGAGG CGCCGATATA CAGGATGGGG GCGGCTGTCA AGAAAACTGA
 4801 TCAATGGGAT CCGAGACAAG CAGAGTGGAA AGACAATCCT GGATTTTCTT AAGTCCGATG
 4861 GATTTGCCAA CCGGAACTTC ATGCAGTTGA TCCATGATGA CTCTCTCACC TTTAAGGAGG
 4921 ACATCCAGAA AGCACAAGTT TCTGGCCAGG GGGACAGTCT TCACGAGCAC ATCGCTAATC
 4981 TTGCAGGTAG CCCAGCTATC AAAAAGGGAA TACTGCAGAC CGTTAAGGTC GTGGATGAAC
 5041 TCGTCAAAGT AATGGGAAGG CATAAGCCCG AGAATATCGT TATCGAGATG GCCCGAGAGA
 5101 ACCAAACTAC CCAGAAGGGA CAGAAGAACA GTAGGGAAAG GATGAAGAGG ATTGAAGAGG
 5161 GTATAAAAGA ACTGGGGTCC CAAATCCTTA AGGAACACCC AGTTGAAAAC ACCCAGCTTC
 5221 AGAATGAGAA GCTCTACCTG TACTACCTGC AGAACGGCAG GGACATGTAC GTGGATCAGG
 5281 AACTGGACAT CAATCGGCTC TCCGACTACG ACGTGGATCA TATCGTGCCC CAGTCTTTTC
 5341 TCAAAGATGA TTCTATTGAT AATAAAGTGT TGACAAGATC CGATAAAAAT AGAGGGAAGA
 5401 GTGATAACGT CCCCTCAGAA GAAGTTGTCA AGAAAATGAA AAATTATTGG CGGCAGCTGC
 5461 TGAACGCCAA ACTGATCACA CAACGGAAGT TCGATAATCT GACTAAGGCT GAACGAGGTG
 5521 GCCTGTCTGA GTTGGATAAA GCCGGCTTCA TCAAAAGGCA GCTTGTTGAG ACACGCCAGA
 5581 TCACCAAGCA CGTGGCCCAA ATTCTCGATT CACGCATGAA CACCAAGTAC GATGAAAATG
 5641 ACAAACTGAT TCGAGAGGTG AAAGTTATTA CTCTGAAGTC TAAGCTGGTC TCAGATTTCA
 5701 GAAAGGACTT TCAGTTTTAT AAGGTGAGAG AGATCAACAA TTACCACCAT GCGCATGATG
 5761 CCTACCTGAA TGCAGTGGTA GGCACTGCAC TTATCAAAAA ATATCCCAAG CTTGAATCTG
 5821 AATTTGTTTA CGGAGACTAT AAAGTGTACG ATGTTAGGAA AATGATCGCA AAGTCTGAGC
 5881 AGGAAATAGG CAAGGCCACC GCTAAGTACT TCTTTTACAG CAATATTATG AATTTTTTCA
 5941 AGACCGAGAT TACACTGGCC AATGGAGAGA TTCGGAAGCG ACCACTTATC GAAACAAACG
 6001 GAGAAACAGG AGAAATCGTG TGGGACAAGG GTAGGGATTT CGCGACAGTC CGGAAGGTCC
 6061 TGTCCATGCC GCAGGTGAAC ATCGTTAAAA AGACCGAAGT ACAGACCGGA GGCTTCTCCA
 6121 AGGAAAGTAT CCTCCCGAAA AGGAACAGCG ACAAGCTGAT CGCACGCAAA AAAGATTGGG
 6181 ACCCCAAGAA ATACGGCGGA TTCGATTCTC CTACAGTCGC TTACAGTGTA CTGGTTGTGG
 6241 CCAAAGTGGA GAAAGGGAAG TCTAAAAAAC TCAAAAGCGT CAAGGAACTG CTGGGCATCA
 6301 CAATCATGGA GCGATCAAGC TTCGAAAAAA ACCCCATCGA CTTTCTCGAG GCGAAAGGAT
 6361 ATAAAGAGGT CAAAAAAGAC CTCATCATTA AGCTTCCCAA GTACTCTCTC TTTGAGCTTG
 6421 AAAACGGCCG GAAACGAATG CTCGCTAGTG CGGGCGAGCT GCAGAAAGGT AACGAGCTGG
 6481 CACTGCCCTC TAAATACGTT AATTTCTTGT ATCTGGCCAG CCACTATGAA AAGCTCAAAG
 6541 GGTCTCCCGA AGATAATGAG CAGAAGCAGC TGTTCGTGGA ACAACACAAA CACTACCTTG
 6601 ATGAGATCAT CGAGCAAATA AGCGAATTCT CCAAAAGAGT GATCCTCGCC GACGCTAACC
 6661 TCGATAAGGT GCTTTCTGCT TACAATAAGC ACAGGGATAA GCCCATCAGG GAGCAGGCAG
 6721 AAAACATTAT CCACTTGTTT ACTCTGACCA ACTTGGGCGC GCCTGCAGCC TTCAAGTACT
 6781 TCGACACCAC CATAGACAGA AAGCGGTACA CCTCTACAAA GGAGGTCCTG GACGCCACAC
 6841 TGATTCATCA GTCAATTACG GGGCTCTATG AAACAAGAAT CGACCTCTCT CAGCTCGGTG
 6901 GAGACAGCAG GGCTGACCCC AAGAAGAAGA GGAAGGTGGC GGCCGCACTC GAGCACCACC
 6961 ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG
 7021 CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT
 7081 TTTTGCTGAA AGGAGGAACT ATATCCGGAT TGGCGAATGG GACGCGCCCT GTAGCGGCGC
 7141 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT
 7201 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG
 7261 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA
 7321 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT
 7381 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG
 7441 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC
 7501 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT
 7561 ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT
 7621 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA ATTAATTCTT AGAAAAACTC
 7681 ATCGAGCATC AAATGAAACT GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG
 7741 AAAAAGCCGT TTCTGTAATG AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG
 7801 ATCCTGGTAT CGGTCTGCGA TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC
 7861 CTCGTCAAAA ATAAGGTTAT CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA
 7921 GAATGGCAAA AGTTTATGCA TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC
 7981 GTCATCAAAA TCACTCGCAT CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG
 8041 ACGAAATACG CGATCGCTGT TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG
 8101 CAGGAACACT GCCAGCGCAT CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC
 8161 CTGGAATGCT GTTTTCCCGG GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG
 8221 GATAAAATGC TTGATGGTCG GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT
 8281 CTCATCTGTA ACATCATTGG CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC
 8341 ATCGGGCTTC CCATACAATC GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC
 8401 CCATTTATAC CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TAGAGCAAGA
 8461 CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG
 8521 TTTTATTGTT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC
 8581 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT
 8641 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA
 8701 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG
 8761 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC
 8821 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG
 8881 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA
 8941 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT
 9001 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG
 9061 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC
 9121 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC
 9181 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC
 9241 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG
 9301 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA
 9361 GCGAGGAAGC GGAAG
//

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