pRSET-CFP

价格:2000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pRSET-CFP
质粒类型: 大肠杆菌表达载体;克隆载体
高拷贝/低拷贝: 高拷贝
克隆方法: 多克隆位点,限制性内切酶
启动子: T7
载体大小: 3.6 kb
5' 测序引物及序列: T7 Forward
3' 测序引物及序列: --
载体标签: His Tag (6x);Xpress Epitope Tag
载体抗性: 氨苄青霉素(Ampicillin)
克隆菌株: DH5alpha,TOP10F'
表达菌株: BL21(DE3)系列,推荐BL21Star(DE3)
备注: pRSET-EmGFP载体的功能是克隆荧光报告基因EmGFP或荧光蛋白EmGFP的表达和纯化,菌体裂解物在紫外灯下肉眼可见荧光。
含 EK (Enterokinase)识别位点
产品目录号: V35220
稳定性: --
组成型/诱导型: 诱导型(IPTG)
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pRSET-CFP载体图谱



pRSET-CFP载体特征

载体简介

载体序列

LOCUS       pRSET/CFP	3600 bp 	DNA	SYN
DEFINITION  pRSET/CFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="pRSET/CFP"
                     /mol_type="other DNA"
     promoter        9..27
                     /label="T7_promoter"
     misc_feature    64..80
                     /label="T7_transl_en_RBS"
     CDS             89..928
                     /label="ORF frame 2"
                     /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK
                     GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
                     TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ
                     LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE
                     LYK*"
     misc_feature    101..118
                     /label="6xHIS"
                     /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK
                     GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
                     TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ
                     LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE
                     LYK*"
     misc_feature    121..139
                     /label="Xpress_fwd_primer"
                     /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK
                     GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
                     TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ
                     LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE
                     LYK*"
     misc_feature    122..154
                     /label="T7_leader"
                     /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK
                     GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
                     TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ
                     LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE
                     LYK*"
     misc_feature    122..154
                     /label="T7 Tag"
                     /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK
                     GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
                     TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ
                     LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE
                     LYK*"
     misc_feature    158..181
                     /label="Xpress_EK"
                     /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK
                     GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
                     TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ
                     LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE
                     LYK*"
     CDS             187..942
                     /label="ORF frame 1"
                     /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR
                     CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP
                     GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY
                     HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR
                     PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*"
     gene            212..925
                     /label="ECFP"
                     /gene="ECFP"
                     /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR
                     CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP
                     GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY
                     HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR
                     PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*"
     misc_feature    complement(254..275)
                     /label="EGFP_N_primer"
                     /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR
                     CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP
                     GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY
                     HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR
                     PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*"
     misc_feature    392..421
                     /label="Y66W (ECFP)"
                     /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR
                     CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP
                     GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY
                     HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR
                     PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*"
     misc_feature    862..883
                     /label="EGFP_C_primer"
                     /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR
                     CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP
                     GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY
                     HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR
                     PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*"
     terminator      948..1076
                     /label="T7_terminator"
                     /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR
                     CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP
                     GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY
                     HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR
                     PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*"
     misc_feature    complement(990..1008)
                     /label="T7_Terminal_primer"
                     /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR
                     CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP
                     GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY
                     HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR
                     PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*"
     rep_origin      1165..1471
                     /label="f1_origin"
                     /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR
                     CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP
                     GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY
                     HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR
                     PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*"
     promoter        1664..1692
                     /label="AmpR_promoter"
                     /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR
                     CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP
                     GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY
                     HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR
                     PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*"
     gene            1734..2594
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR
                     CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP
                     GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY
                     HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR
                     PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*"
     CDS             1734..2594
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      2749..3368
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 CGCGAAATTA ATACGACTCA CTATAGGGAG ACCACAACGG TTTCCCTCTA GAAATAATTT
   61 TGTTTAACTT TAAGAAGGAG ATATACATAT GCGGGGTTCT CATCATCATC ATCATCATGG
  121 TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG ATGACGATAA
  181 GGATCGATGG GGATCCGAAT TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG
  241 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAGGT TCAGCGTGTC
  301 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC
  361 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTGGG GCGTGCAGTG
  421 CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA
  481 AGGCTACGTC CAGGAGCGTA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC
  541 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT
  601 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACATCA GCCACAACGT
  661 CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC CACTTCAAGA TCCGCCACAA
  721 CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA
  781 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA
  841 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
  901 TCTCGGCATG GACGAGCTGT ACAAGTAACT CGAGAAGCTT GATCCGGCTG CTAACAAAGC
  961 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG
 1021 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATCTGG
 1081 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC
 1141 GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC
 1201 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT
 1261 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC
 1321 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT
 1381 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT
 1441 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT
 1501 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA
 1561 AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAGGTGGC ACTTTTCGGG
 1621 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC
 1681 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA
 1741 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG
 1801 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG
 1861 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC
 1921 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG
 1981 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT
 2041 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG
 2101 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC
 2161 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT
 2221 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG
 2281 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC
 2341 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC
 2401 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA
 2461 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG
 2521 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA
 2581 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC
 2641 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA
 2701 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT
 2761 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC
 2821 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG
 2881 GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC
 2941 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG
 3001 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG
 3061 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA
 3121 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG
 3181 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA
 3241 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT
 3301 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA
 3361 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC
 3421 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG
 3481 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC
 3541 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGG ATCTCGATCC

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