pCMV-RFP-C-HA

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pCMV-RFP-C-HA
质粒类型: 哺乳动物表达载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 4723 bp
5' 测序引物及序列: CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 测序引物及序列: BGH rev 5''TAGAAGGCACAGTCGAGG3''
载体标签: RFP、HA (C ter)
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: DH5α
宿主细胞(系): 哺乳动物细胞系
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCMV-RFP-C-HA载体图谱



pCMV-RFP-C-HA多克隆位点

pCMV-RFP-C-HA载体特征

载体简介

载体序列

LOCUS       Exported                 bp ds-DNA     circular SYN 12-AUG-2016
KEYWORDS    pCMV-RFP-C-HA
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Biofeng Lab.
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        67..370
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        625..643
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    650..652
                     /note="ATG"
     misc_feature    659..696
                     /note="MCS"
     gene            707..1399
                     /note="RFP"
     CDS             1400..1426
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     polyA_signal    1456..1581
                     /note="bGH poly(A)"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1771..2077)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2121..2225
                     /gene="bla"
                     /note="bla promoter"
     promoter        2425..2584
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      2435..2570
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             2619..3413
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3649..3692
                     /note="HSV TK poly(A)"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3998..4641
                     /direction=RIGHT
                     /note="pUC"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tcccgggagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      601 ttactggctt atcgaaattc tagataatac gactcactat agggcgaata tggccacggg
      661 cgcgccctgc aggctagcct cgagggatcc gatatcgcgg ccgcttcttt ccaaacaagt
      721 tttgcctcat gatgtgaaaa tgagatacca tatggatgga tgtgttaacg gtcacagttt
      781 cactattgag ggagaaggtg ctggtaaacc ttatgaaggc aaaaagatct tggaactgcg
      841 tgtaacaaag ggaggaccac ttccattcgc ctttgacatt ttgtccagcg ttttcactta
      901 tggtaacagg tgtttctgtg agtatccaga ggatatgccc gactacttta agcaatcatt
      961 gcccgagggt cactcttggg agagaacttt aatgtttgag gatggaggat gcggtactgc
     1021 ttcagcccat atatcgttgg acaaaaactg tttcgtgcat aagtctacgt ttcatggtgt
     1081 gaattttcct gctaatggtc cggtcatgca gaaaaaggca atgaattggg agcctagctc
     1141 tgaactgatc accgcatgtg atggtattct caaaggcgac gttaccatgt ttttgttgct
     1201 ggaaggtggt cacagattaa agtgccaatt cacaacatcc tacaaggctc ataaagcagt
     1261 gaagatgcca ccaaatcaca ttatcgagca tgtcctagtc aaaaaggaag ttgctgacgg
     1321 ttttcaaatc caggaacacg ctgttgcaaa acacttcaca gtagatgtta aggaaactcg
     1381 cggccgcgaa ttcagatctt acccctacga cgtgcccgac tacgccgtta acttatgata
     1441 aggccggcct gatcaaaaat cagcctcgac tgtgccttct agttgccagc catctgttgt
     1501 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1561 ataaaatgag gaaattgcat ctgtacatct agcattcagt tgtggtttgt ccaaactcat
     1621 caatttatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1681 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1741 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     1801 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     1861 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     1921 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     1981 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2041 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     2101 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2161 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2221 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     2281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     2341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     2461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     2521 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     2581 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     2641 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     2701 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     2761 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     2821 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     2881 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     2941 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     3001 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     3061 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     3121 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     3181 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3241 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     3301 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     3361 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     3421 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     3481 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     3541 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     3601 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     3661 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     3721 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     3781 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     3841 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     3901 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3961 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4021 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4081 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4141 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4201 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4261 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4321 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4381 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4441 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4501 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4561 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4621 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4681 gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//

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