| 出品公司: | Stratagene |
|---|---|
| 载体名称: | pBluescript KS(+) |
| 质粒类型: | 细菌表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶 |
| 启动子: | -- |
| 载体大小: | 2958 bp |
| 5' 测序引物及序列: | M13 fwd |
| 3' 测序引物及序列: | M13 rev |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 标准克隆载体（从lambda ZAP中分离的噬菌体）。f1+的方向可以拯救感觉链ssDNA。pBluescript KS（+）和pBluescript SK（+）因MCS的方向不同而不同。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pBluescript KS(+)载体质粒基本信息
pBluescript KS(+)质粒图谱载体图谱和pBluescript KS(+)载体序列质粒序列多克隆位点信息
载体图谱1.png)
pBluescript KS(+)质粒载体简介
pBluescript KS(+)质粒序列载体序列
LOCUS pBluescript KS(+) 2958 bp ds-DNA circular SYN 25-NOV-2013
DEFINITION Standard cloning vector (phagemid excised from lambda ZAP). The f1
(+) orientation allows rescue of sense strand ssDNA. pBluescript
SK(+) has a reversed MCS.
ACCESSION X52331
VERSION .
KEYWORDS pBluescript KS(+)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2958)
AUTHORS Short JM, Fernandez JM, Sorge JA, Huse WD.
TITLE Lambda ZAP: a bacteriophage lambda expression vector with in vivo
excision properties
JOURNAL Nucleic Acids Res. 16 (15), 7583-7600 (1988)
PUBMED 2970625
REFERENCE 2 (bases 1 to 2958)
AUTHORS Stratagene
TITLE Direct Submission
JOURNAL Exported Saturday, January 11, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2958
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
rep_origin complement(6..461)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(244..816)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSSKLTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPLV
LERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGF
PRQALNRGLPLGFRFSALRHLDPKKLD"
primer_bind 603..619
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 653..760
/note="MCS"
/note="pBluescript multiple cloning site"
primer_bind 677..693
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(727..743)
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(773..791)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(812..828)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 836..852
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(860..890)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(1214..1802)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1973..2833)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2834..2938)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
721 gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt
781 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
1921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
1981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
2041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
2101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
2161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
2221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
2281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
2341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
2401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
2461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
2521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
2581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
2641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
2701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
2761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
2821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
2881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
2941 catttccccg aaaagtgc
//
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