pAAV-cFos2-mAvicFP1-iPAK4

LOCUS       pAAV-cFos2-mAvicFP1-iPAK4                6653 bp DNA     circular SYN 23-APR-2024
DEFINITION  Express FlicR1 preferentially in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul 
            A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE
  TITLE     A Bright and Fast Red Fluorescent Protein Voltage Indicator That 
            Reports Neuronal Activity in Organotypic Brain Slices.
  JOURNAL   J Neurosci. 2016 Feb 24;36(8):2458-72. doi: 
            10.1523/JNEUROSCI.3484-15.2016.
  PUBMED    26911693
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3484-15"; journalName: "J Neurosci"; date: 
            "2016-02-24- 24"; volume: "36"; issue: "8"; pages: "2458-72"
FEATURES             Location/Qualifiers
     source          1..6653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2074..3150
                     /mol_type="genomic DNA"
                     /organism="."
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        265..295
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   381..510
                     /label=AAV2 ITR
     misc_feature    577..1337
                     /label=cfos-Luo liqun
     CDS             complement(1277..1288)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(1336..1359)
                     /label=MCS-cfos-R
     primer_bind     1338..1375
                     /label=Primer 1
     CDS             1357..2073
                     /codon_start=1
                     /product="https://www.addgene.org/browse/article/28203833/"
                     /label=mAvicFP1
                     /translation="MVSKGEELFNGIVPILIELNGDVHGHKFSVRGEGEGDAGSGKIEI
                     KFVCTTGTLPVPWPTLVTTLCYGVQCFTRYPEHMKQHDFYKSAMPDGYIQKRTISFQDD
                     GHYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGNKMEYNYNSHSVYVLSDKANNGIK
                     VNFKIRHNLKGEGIQLADHDQQNIPIGDGPVLLPDYHYLSTQTKITKDPNEKRDHMNLV
                     EFVTACGITHGMDELYK"
     regulatory      1357..1360
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2053..2099
                     /label=GFP-iPAK4-F
     primer_bind     2053..2099
                     /label=Primer 1a
     CDS             2086..2199
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             2206..3147
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(2299..2317)
                     /label=PAK4-R
     primer_bind     complement(3130..3172)
                     /label=HindIII-PAK4-R
     misc_feature    3163..3751
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3216..3236)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3634..3645)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3754..3770)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3755..3771)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3800..3921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3811..3830)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3865..3884
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3903..3921)
                     /label=SV40-polyA
     repeat_region   4083..4212
                     /label=AAV2 ITR
     primer_bind     complement(4231..4248)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4231..4247)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4240..4262)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4389..4844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4476..4495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4686..4707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4870..4974
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4975..5835
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5193..5212)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6006..6594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6495..6514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccagatt
      361 taattaaggc cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc
      421 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg
      481 gagtggccaa ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact
      541 tatctacgta gccatgctct aggaagatct ctgcagaagc tttcctttag gaacagaggc
      601 ttcgagcctt taaggctgcg tacttgcttc tcctaatacc agagactcaa aaaaaaaaaa
      661 aaagttccag attgctggac aatgacccgg gtctcatccc ttgaccctgg gaaccgggtc
      721 cacattgaat caggtgcgaa tgttcgctcg ccttctctgc ctttcccgcc tcccctcccc
      781 cggccgcggc cccggttccc cccctgcgct gcaccctcag agttggctgc agccggcgag
      841 ctgttcccgt caatccctcc ctcctttaca caggatgtcc atattaggac atctgcgtca
      901 gcaggtttcc acggccggtc cctgttgttc tggggggggg accatctccg aaatcctaca
      961 cgcggaaggt ctaggagacc ccctaagatc ccaaatgtga acactcatag gtgaaagatg
     1021 tatgccaaga cgggggttga aagcctgggg cgtagagttg acgacagagc gcccgcagag
     1081 ggccttgggg cgcgcttccc cccccttcca gttccgccca gtgacgtagg aagtccatcc
     1141 attcacagcg cttctataaa ggcgccagct gaggcgccta ctactccaac cgcgactgca
     1201 gcgagcaact gagaagactg gatagagccg gcggttccgc gaacgagcag tgaccgcgct
     1261 cccacccagc tctgctctgc agctcccacc agtgtctacc cctggacccc ttgccgggct
     1321 ttccccaaac ttcgacctcg aattcgggga tccaccatgg tgagcaaggg cgaggagctg
     1381 ttcaacggta tcgttcctat cctgatcgaa ctgaacggtg acgttcatgg tcataagttc
     1441 tctgttcgtg gtgaaggtga aggtgacgct ggttctggta agatcgaaat caagttcgtt
     1501 tgcaccaccg gtaccctgcc tgttccttgg cctaccctgg ttaccacctt atgctacggt
     1561 gttcagtgct tcacccgtta ccctgaacat atgaagcagc atgacttcta caagtctgct
     1621 atgcctgacg gttacatcca gaagcgtacc atctctttcc aggacgacgg tcattacaag
     1681 acccgtgctg aagttaagtt cgaaggtgac accctggtta accgtatcga actgaagggt
     1741 atcgacttca aggaagacgg taacatcctg ggtaacaaga tggaatacaa ctacaactct
     1801 cattctgttt acgttctgtc tgacaaggct aacaacggta tcaaggttaa cttcaagatc
     1861 cgtcataacc tgaagggtga aggtatccag ctggctgacc atgaccagca gaacatccct
     1921 atcggtgacg gtcctgttct gctgcctgac taccattacc tgtctaccca gaccaagatc
     1981 accaaggacc ctaacgaaaa gcgtgaccat atgaacctgg ttgaattcgt taccgcttgc
     2041 ggtatcaccc atggcatgga cgagctgtac aagtcaggtg gatccgaagc agaggactgg
     2101 acagcagccc tactgaacag gggtcgcagt cgccagcccc tggtactagg ggacaattgc
     2161 tttgctgact tggtgcacaa ctggatggag ctgcctgagg aattccccgc cgcccctgct
     2221 gttcctgggc cccctggccc ccgctcacca cagcgggagc cacagcgagt atcccatgag
     2281 cagttccggg ctgccctgca gctggtggtg gacccaggcg acccccgctc ctacctggac
     2341 aacttcatca agattggcga gggctccacg ggcatcgtgt gcatcgccac cgtgcgcagc
     2401 tcgggcaagc tggtggccgt caagaagatg gacctgcgca agcagcagag gcgcgagctg
     2461 ctcttcaacg aggtggtaat catgagggac taccagcacg agaatgtggt ggagatgtac
     2521 aacagctacc tggtggggga cgagctctgg gtggtcatgg agttcctgga aggaggcgcc
     2581 ctcaccgaca tcgtcaccca caccaggatg aacgaggagc agatcgcagc cgtgtgcctt
     2641 gcagtgctgc aggccctgtc ggtgctccac gcccagggcg tcatccaccg ggacatcaag
     2701 agcgactcga tcctgctgac ccatgatggc agggtgaagc tgtcagactt tgggttctgc
     2761 gcccaggtga gcaaggaagt gccccgaagg aagtcgctgg tcggcacgcc ctactggatg
     2821 gccccagagc tcatctcccg ccttccctac gggccagagg tagacatctg gtcgctgggg
     2881 ataatggtga ttgagatggt ggacggagag cccccctact tcaacgagcc acccctcaaa
     2941 gccatgaaga tgattcggga caacctgcca ccccgactga agaacctgca caaggtgtcg
     3001 ccatccctga agggcttcct ggaccgcctg ctggtgcgag accctgccca gcgggccacg
     3061 gcagccgagc tgctgaagca cccattcctg gccaaggcag ggccgcctgc cagcatcgtg
     3121 cccctcatgc gccagaaccg caccagatga aagcttatcg ataatcaacc tctggattac
     3181 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3241 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3301 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3361 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3421 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3481 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3541 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     3601 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3661 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3721 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg acccgggcgg
     3781 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     3841 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     3901 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     3961 ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     4021 aaggatcttc ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta
     4081 caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     4141 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4201 gcgagcgcgc agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg
     4261 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4321 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4381 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4441 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4501 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4561 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4621 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4681 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4741 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4801 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     4861 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4921 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4981 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5041 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5101 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5161 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5221 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5281 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5341 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5401 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5461 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5521 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5581 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5641 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5701 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5761 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5821 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5881 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5941 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6001 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6061 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6121 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6361 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6601 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca cat
//