| 质粒名称: | pAAVone-AAV-PHP.eB-CMV-eGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 230929 |
| 存储实验室: | Qizhao Wang |
|
载体骨架基本信息 |
|
| 载体名称: | pAAVone |
| 载体出品公司: | Clontech |
| 空载体大小: | 15817 bp |
| 完整质粒大小: | 15817bp |
| 载体类型: | AAV |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | eGFP |
| 物种来源: | Synthetic |
| 启动子: | CMV |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | pAAVone-AAV-PHP.eB-CMV-eGFP was a gift from Qizhao Wang (Addgene plasmid # 230929 ; http://n2t.net/addgene:230929 ; RRID:Addgene_230929) |
| 参考文献部分: | AAVone: A cost-effective, single-plasmid solution for efficient AAV production with reduced DNA impurities. Yang R, Tran NT, Chen T, Cui M, Wang Y, Sharma T, Liu Y, Zhang J, Yuan X, Zhang D, Chen C, Shi Z, Wang L, Dai Y, Zaidi H, Liang J, Chen M, Jaijyan D, Hu H, Wang B, Xu C, Hu W, Gao G, Yu D, Tai PWL, Wang Q. Mol Ther Nucleic Acids. 2025 May 14;36(2):102563. doi: 10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10. 10.1016/j.omtn.2025.102563 PubMed 40503174 |
买家导航
pAAVone-AAV-PHP.eB-CMV-eGFP载体质粒基本信息
pAAVone-AAV-PHP.eB-CMV-eGFP质粒图谱载体图谱和pAAVone-AAV-PHP.eB-CMV-eGFP载体序列质粒序列多克隆位点信息
pAAVone-AAV-PHP.eB-CMV-eGFP质粒载体简介
pAAVone-AAV-PHP.eB-CMV-eGFP质粒序列载体序列
LOCUS pAAVone-AAV-PHP.eB-CMV-eGFP 15817 bp DNA circular SYN 15-DEC-2025
DEFINITION pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB
serotype, utilizing the AAVone single-plasmid AAV production
system..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15817)
AUTHORS Yang R, Tran NT, Chen T, Cui M, Wang Y, Sharma T, Liu Y, Zhang J,
Yuan X, Zhang D, Chen C, Shi Z, Wang L, Dai Y, Zaidi H, Liang J,
Chen M, Jaijyan D, Hu H, Wang B, Xu C, Hu W, Gao G, Yu D, Tai PWL,
Wang Q
TITLE AAVone: A cost-effective, single-plasmid solution for efficient AAV
production with reduced DNA impurities.
JOURNAL Mol Ther Nucleic Acids. 2025 May 14;36(2):102563. doi:
10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10.
PUBMED 40503174
REFERENCE 2 (bases 1 to 15817)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther
Nucleic Acids."; date: "2025-05-14"; volume: "36(2):102563. doi";
pages: " 10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10"
COMMENT Sequence Label: pAAVone-AAV-PHP.eB-CMV-eGFP
FEATURES Location/Qualifiers
source 1..15817
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 134..512
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 667..687
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
intron 717..813
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
regulatory 818..827
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(824..826,827..829,830..1543)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(824..826,827..829,830..1540)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(869..890)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1130..1149)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1477..1498
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 1568..2156
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(1621..1641)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2039..2050)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2188..2664
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(2387..2406)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 2673..2802
/label=AAV2 ITR
CDS complement(4050..4061)
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
rep_origin 13534..13988
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(13620..13639)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 13830..13851
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 14014..14118
/gene="bla"
/label=AmpR promoter
CDS 14119..14913
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 14119..14913
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(14173..14192)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 14783..14802
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
rep_origin 15084..15672
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 15573..15592
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc
61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
121 cggccgcacg cgtacattga ttattgacta gttattaata gtaatcaatt acggggtcat
181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc acctgcgtaa
721 gtttagtctt tttgtctttt atttcaggtc ccggatccgg tggtggtgca aatcaaagaa
781 ctgctcctca gtggatgttg cctttacttc taggcgcgcc accatggtga gcaagggcga
841 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
901 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
961 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
1021 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
1081 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
1141 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
1201 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
1261 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
1321 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
1381 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
1441 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
1501 cgccgccggg atcactctcg gcatggacga gctgtacaag taacgtacgg atatcaagct
1561 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
1621 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
1681 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
1741 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
1801 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
1861 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
1921 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
1981 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
2041 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
2101 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
2161 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
2221 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
2281 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
2341 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
2401 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
2461 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
2521 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
2581 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
2641 accactgctc ccttccctgt ccttttaatt aaaggaaccc ctagtgatgg agttggccac
2701 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
2761 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgccaac cggtgtcgac
2821 gtaatccgta gatgtacctg gacatccagg tgatgccggc ggcggtggtg gaggcgcgcg
2881 gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa aaagtgctcc atggtcggga
2941 cgctctggcc ggtgaggcgt gcgcagtcgt tgacgctcta gaccgtgcaa aaggagagcc
3001 tgtaagcggg cactcttccg tggtctggtg gataaattcg caagggtatc atggcggacg
3061 accggggttc gaaccccgga tccggccgtc cgccgtgatc catgcggtta ccgcccgcgt
3121 gtcgaaccca ggtgtgcgac gtcagacaac gggggagcgc tccttttggc ttccttccag
3181 gcgcggcggc tgctgcgcta gcttttttgg ccactggccg cgcgcggcgt aagcggttag
3241 gctggaaagc gaaagcatta agtggctcgc tccctgtagc cggagggtta ttttccaagg
3301 gttgagtcgc aggacccccg gttcgagtct cgggccggcc ggactgcggc gaacgggggt
3361 ttgcctcccc gtcatgcaag accccgcttg caaattcctc cggaaacagg gacgagcccc
3421 ttttttgctt ttcccagatg catccggtgc tgcggcagat gcgcccccct cctcagcagc
3481 ggcaagagca agagcagcgg cagacatgca gggcaccctc cccttctcct accgcgtcag
3541 gaggggcaac atcctacatc gatagatctg cagaattcgg cttggcggct gcgcgttcaa
3601 acctcccgct tcaaaatgga gaccctgcgt gctcactcgg gcttaaatac ccagcgtgac
3661 cacatggtgt cgcaaaatgt cgcaaaacac tcacgtgacc tctaatacag gacctctaga
3721 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaaag ccgaattcca
3781 gcacactggc ggccgttact agtggatcct agagcatgga aactagataa gaaagaaata
3841 cgcagagacc aaagttcaac tgaaacgaat taaacggttt attgattaac aagcgatcgt
3901 tattacagat tacgagtcag gtatctggtg ccaatggggc ggggttcact atatacacct
3961 tcagtattaa cagcaaattc aacattatta gacttgtaat agttggaagt gtactggatc
4021 tccgggttcc agcgcttgct gttttccttc tgcagctccc actcgatctc cacgctgact
4081 tggccagtag aatactgggt gatgaaagag ttcagcttgt ccttgttgaa ggccgttgga
4141 ggatccgcag gtacaggtgt gtttttgatg aggatctgag gaggcgggtg cttcattcca
4201 aaccctccca tcagcggaga agggtgaaag ttgccgtccg tgtgaggaat tttggcccaa
4261 atgggtcctt gcaggtacac atctctgtcc tgccaaacca tacccggaag tattccttgg
4321 ttttgaaccc agccggtctg cgcctgtgcc ttaaaaggca ccgccaaagt cccatcactc
4381 tggtggtttg tggccacttg tccataggac tccgttgcta ccgggttagt agttttaatt
4441 tcttcttcgt tggttatcat gactttgtcc gcatccacgt tgtctcttcc agttccttgt
4501 ttgccaaaaa ttaaagatcc agacaaagga aagaaacggt cctctccttc tttgtggctg
4561 gccatagcag gtccaggatt catcaagcta ttacgtccat tgagagccca agaagaagct
4621 ccaggccaag caaattcgct gttgttgttt tgagtcacag tggttgagac acgttgttgt
4681 cggtagctgg gtccaggtat gtagtttctt ccctggacag ccatgttgct gggtccggcc
4741 acactgaatt ttagcgtttg ttgattctgt ccagaaccgt taatagtctt tgagagatag
4801 tacaagtatt ggtcgatgag tggattcatt agtcggtcca ggctttggct gtgagcgtag
4861 ctgctatgga aaggtacgtt ctcaaactcg tagctgaact ggaagttgtt acccgttctt
4921 agcatttgcg acgggaaata ttccaggcag taaaaggacg aacgacccac ggcctggctt
4981 ccatcattaa gcgtcagata cccgtactga ggaatcatga aaacgtccgc tgggaacggc
5041 gggaggcagc cctcgtgagc cgacccgagc acgtacggga gctgatagtc tgagtccgtg
5101 aagacctgga ccgtgctggt aaggttattg gcgatggtct tgactccatt gttgtccgta
5161 acctctttga cctgaatgtt gaagagcttg aagttgagtc gcttaggccg gaatccccag
5221 ttgttgttga tgagtcgctg ccagtcacgt ggtgagaagt ggcagtggaa tctgttgaag
5281 tcaaaatacc cccagggggt gctgtagccg aagtaggcgt tgtcatttga agatcctcca
5341 gatgtgctgt tggagatttg cttgtagagg tgattgttgt aggtgggcag ggcccaggtt
5401 cgggtgctgg tggtgatgac tctgtccccc agccattggg aatcgcaatg ccaatttccc
5461 gaggaactac ccactccatc ggcaccttcg ttattgtctg ccactggtgc gccaccacct
5521 gaagccattg taagagatcc cacacctgag ggggctgcgg gaggttctcc gattggttga
5581 gggtctggga ctgactctgt gtcgccagtc tgaccgaaat tgagtctctt tttagcgggc
5641 tgtgcacccg atttgccaat acccgcggag gagtccggtt cctgaggaga ctgctctaca
5701 ggcctcttct ttccaggagc cgtcttagcc gcttcctcaa ccagaccaag aggttcaaga
5761 agcctctttt tggcctggaa gactgctcgc ccgaggttgc ccccaaaaga cgtatcttct
5821 ttgagccgct cctggaactc ggcgtcggcg tggttgtact tgaggtacgg gttgtctccg
5881 gccttgagct gctggtcgta ggccttgtcg tgctcgaggg ccgccgcgtc tgctgcgttg
5941 accggctccc ccttgtcgag tccgttgccg ggtccaaggt atttgtaacc cggaagcaca
6001 agacctcgag cgttgtcttg atgttgttga tttgccttgg gttgaggggc tccaggtttc
6061 aaagcccacc actcgcgaat tccttcacta aggttgtcct cgagccaatc tggaagataa
6121 ccatcggcag ccatacctga tttaaatcat ttattgttca aagatgcagt catccaaatc
6181 cacattgacc agatcgcagg cagtgcaagc gtctggcacc tttcccatga tatgatgaat
6241 gtagcacagt ttctgatacg cctttttgac gacagaaacg ggttgagatt ctgacacggg
6301 aaagcactct aaacagtctt tctgtccgtg agtgaagcag atatttgaat tctgattcat
6361 tctctcgcat tgtctgcagg gaaacagcat cagattcatg cccacgtgac gagaacattt
6421 gtttacgtac ctgtctgcgt agttgatcga agcttccgcg tctgacgtcg atggctgcgc
6481 aactgactcg cgcacccgtt tgggctcact tatatctgcg tcactggggg cgggtctttt
6541 cttggctcca ccctttttga cgtagaattc atgctccacc tcaaccacgt gatcctttgc
6601 ccaccggaaa aagtctttga cttcctgctt ggtgaccttc ccaaagtcat gatccagacg
6661 gcgggtgagt tcaaatttga acatccggtc ttgcaacggc tgctggtgtt cgaaggtcgt
6721 tgagttcccg tcaatcacgg cgcacatgtt ggtgttggag gtgacgatca cgggagtcgg
6781 gtctatctgg gccgaggact tgcatttctg gtccacgcgc accttgcttc ctccgagaat
6841 ggctttggcc gactccacga ccttggcggt catcttcccc tcctcccacc agatcaccat
6901 cttgtcgaca cagtcgttga agggaaagtt ctcattggtc cagtttacgc acccgtagaa
6961 gggcacagtg tgggctatgg cctccgcgat gttggtcttc ccggtagttg caggcccaaa
7021 cagccagatg gtgttcctct tgccgaactt tttcgtggcc catcccagaa agacggaagc
7081 cgcatattgg ggatcgtacc cgtttagttc caaaatttta taaatccgat tgctggaaat
7141 gtcctccacg ggctgctggc ccaccaggta gtcgggggcg gttttagtca ggctcataat
7201 ctttcccgca ttgtccaagg cagccttgat ttgggaccgc gagttggagg ccgcattgaa
7261 ggagatgtat gaggcctggt cctcctggat ccactgcttc tccgaggtaa tccccttgtc
7321 cacgagccac ccgaccagct ccatgtacct ggctgaagtt tttgatctga tcaccggcgc
7381 atcagaattg ggattctgat tctctttgtt ctgctcctgc gtctgcgaca cgtgcgtcag
7441 atgctgcgcc accaaccgtt tacgctccgt gagattcaaa caggcgctta aatactgttc
7501 catattagtc cacgcccact ggagctcagg ctgggttttg gggagcaagt aattggggat
7561 gtagcactca tccaccacct tgttcccgcc tccggcgcca tttctggtct ttgtgaccgc
7621 gaaccagttt ggcaaagtcg gctcgatccc gcggtaaatt ctctgaatca gtttttcgcg
7681 aatctgactc aggaaacgtc ccaaaaccat ggatttcacc ccggtggttt ccacgagcac
7741 gtgcatgtgg aagtagctct ctcccttctc aaattgcaca aagaaaagag cctccggtgc
7801 cttactcaca cggcgccatt ccgtcagaaa gtcgcgctgc agcttctcgg ccacggtcag
7861 gggtgcctgc tcaatcagat tcagatccat gtcagaatct ggcggcaact cccattcctt
7921 ctcggccacc cagttcacaa agctgtcaga aatgccgggc agatgctcgt caaggtcgct
7981 ggggacctta atcacaatct cgtaaaaccc cggcatggcg gctgctcgtt caaatttcgc
8041 ggtcaaaatg gaggttgcat cctgctcgct cactcggcca tatatacctc agcgtgacca
8101 catggtgtcg caaaatgtcg caaaacactc acgttgacct ctaatacagg accgcggccg
8161 ctctagtgaa tccacagaaa ctagcgaggt aagcacttac tctatgtctt ttacatggtc
8221 ctgggaaagt ggaaaataca ccactgaaac ttttgctacc aactcttaca ccttctccta
8281 cattgcccag gaataaagaa tcgtgaacct gttgcatgtt atgtttcaac gtgtttattt
8341 ttcaattgca gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata
8401 gcttatattg atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac
8461 ctccctccca acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca
8521 tatcatgggt aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca
8581 aacgctcatc agtgatatta ataaactccc cgggcagctc gcttaagttc atgtcgctgt
8641 ccagctgctg agccacaggc tgctgtccaa cttgcggttg ctcaacgggc ggcgaagggg
8701 aagtccatgc ctacatgggg gtagagccat aatcgtgcat caggataggg cggtggtgct
8761 gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg
8821 cagtggtctc ctcagcgatg attcgcaccg cccgcagcat gagacgcctt gtcctccggg
8881 cacagcagcg caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa
8941 tattgttcaa aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag
9001 aacccacgtg gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca
9061 cgctggacat aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata
9121 taaacctctg attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct
9181 gcccgccggc tatgcactgc agggaaccgg gactggaaca atgacagtgg agagcccagg
9241 actcgtaacc atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca
9301 cgtgcataca cttcctcagg attacaagct cctcccgcgt cagaaccata tcccagggaa
9361 caacccattc ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca
9421 cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag
9481 cgcgggtctc tgtctcaaaa ggaggtaggc gatccctact gtacggagtg cgccgagaca
9541 accgagatcg tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc
9601 ctgaagcaaa accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgtcgctta
9661 gctcgctctg tgtagtagtt gtagtatatc cactctctca aagcatccag gcgcctaggc
9721 aaaatagcac cctcccgctc cagaacaaca tacagcgctt ccacagcggc agccataaca
9781 gtcagcctta ccagtaaaaa aacctattaa aaaacaccac tcgacacggc accagctcaa
9841 tcagtcacag tgtaaaaagg gccaagtaca gagcgagtat atataggact aaaaaatgac
9901 gtaacggtta aagtccacaa aaaacaccca gaaaaccgca cgcgaaccta cgcccagaaa
9961 cgaaagccaa aaaacccaca acttcctcaa atcttcactt ccgttttccc acgatacgtc
10021 acttcccatt ttaaaaaaac tacaattccc aatacatgca agttactccg ccctaaaacg
10081 cccgggcgac cgcaccctgt gacgaaagcc gcccgcaagc tgcgcccctg agttagtcat
10141 ctgaacttcg gcctgggcgt ctctgggaag taccacagtg gtgggagcgg gactttcctg
10201 gtacaccagg gcagcgggcc aactacgggg attaaggtta ttacgaggtg tggtggtaat
10261 agccgcctgt tcgaggagaa ttcggtttcg gtgggcgcgg attccgttga cccgggatat
10321 catgtggggt cccgcgctca tgtagtttat tcgggttgag tagtcttggg cagctccagc
10381 cgcaagtccc atttgtggct ggtaactcca catgtagggc gtgggaattt ccttgctcat
10441 aatggcgctg acgacaggtg ctggcgccgg gtgtggccgc tggagatgac gtagttttcg
10501 cgcttaaatt tgagaaaggg cgcgaaacta gtccttaaga gtcagcgcgc agtatttgct
10561 gaagagagcc tccgcgtctt ccagcgtgcg ccgaagctga tcttcgcttt tgtgatacag
10621 gcagctgcgg gtgagggagc gcagagacct gttttttatt ttcagctctt gttcttggcc
10681 cctgctttgt tgaaatatag catacagagt gggaaaaatc ctatttctaa gctcgcgggt
10741 cgatacgggt tcgttgggcg ccagacgcag cgctcctcct cctgctgctg ccgccgctgt
10801 ggatttcttg ggctttgtca gagtcttgct atccggtcgc ctttgcttct gtgtgaccgc
10861 tgctgttgct gccgctgccg ctgccgccgg tgcagtaggg gctgtagaga tgacggtagt
10921 aatgcaggat gttacggggg aaggccacgc cgtgatggta gagaagaaag cggcgggcga
10981 aggagatgtt gcccccacag tcttgcaagc aagcaactat ggcgttcttg tgcccgcgcc
11041 acgagcggta gccttggcgc tgttgttgct cttgggctaa cggcggcggc tgcttagact
11101 taccggccct ggttccagtg gtgtcccatc tacggttggg tcggcgaaca ggcagtgccg
11161 gcggcgcctg aggagcggag gttgtagcga tgctgggaac ggttgccaat ttctggggcg
11221 ccggcgaggg gaatgcgacc gagggtgacg gtgtttcgtc tgacacctct tcggcctcgg
11281 aagcttcgtc taggctgtcc cagtcttcca tcatctcctc ctcctcgtcc aaaacctcct
11341 ctgcctgact gtcccagtat tcctcctcgt ccgtgggtgg cggcggcggc agctgcagct
11401 tctttttggg tgccatcctg ggaagcaagg gcccgcggct gctgataggg ctgcggcggc
11461 ggggggattg ggttgagctc ctcgccggac tgggggtcca ggtaaacccc ccgtcccttt
11521 cgtagcagaa actcttggcg ggctttgttg atggcttgca attggccaag gatgtggccc
11581 tgggtaatga cgcaggcggt aagctccgca tttggcgggc gggattggtc ttcgtagaac
11641 ctaatctcgt gggcgtggta gtcctcaggg agaaggaaat ggccagtcgg gaagaggagc
11701 agcgcgaaac cacccccgag cgcggacgcg gtgcggcgcg acgtccacca accatggagg
11761 acgtgtcgtc cccgtcgccg tcgccgccgc ctccccgcgc gcccccaaaa aagagactgc
11821 ggagacgcct ggagtccgag gacgaggaag acagttccca ggacgctctg gtgcctagga
11881 caccatctcc aagaccctcc acttccaccg ccgacctggc cattgccagc aaaaaaaaga
11941 agaagaggcc ctcccctaaa cctgaacgac cacccagccc agaagtgatc gtggactcag
12001 aggaagagcg cgaggacgtc gctctgcaga tggtgggatt cagcaatcca cctgtgctca
12061 tcaagcacgg caaaggggga aaacggaccg tgcgcaggct gaacgaggat gatcctgtgg
12121 caaggggcat gaggacacag gaggagaagg aagaatcctc cgaggccgag agcgagagca
12181 cagtgattaa tccactgtcc ctgccaattg tgagcgcttg ggagaaggga atggaggccg
12241 cccgggccct gatggacaag taccacgtgg acaacgacct gaaggctaat ttcaaactgc
12301 tgcctgacca ggtggaggcc ctggctgccg tgtgcaagac atggctgaat gaggagcata
12361 gagggctgca gctgaccttt acatcaaaca agacctttgt gaccatgatg ggcaggtttc
12421 tgcaggccta cctgcaatct ttcgccgaag tgacttacaa gcaccacgag cccaccggct
12481 gcgccctgtg gctgcacaga tgcgccgaga ttgagggcga gctgaaatgc ctgcacggct
12541 ctatcatgat taacaaggag cacgtgatcg agatggatgt gacttccgaa aacggccagc
12601 gcgccctgaa ggagcagtcc agcaaggcca agatcgtgaa gaacaggtgg ggcaggaacg
12661 tggtgcagat ttctaacaca gacgccagat gttgcgtgca tgacgccgcc tgccctgcca
12721 atcagtttag cggcaagagc tgcggaatgt tcttcagcga gggagccaag gcgcaagtgg
12781 ccttcaagca gatcaaggcc ttcatgcaag ccctgtatcc aaatgcccag accggccacg
12841 gccacctcct gatgcccctg cgctgcgaat gtaactccaa gcccggccac gcccctttcc
12901 tgggcaggca gctgccaaaa ctgacccctt ttgccctgag caacgccgaa gatctggatg
12961 ccgatctgat cagcgacaag tccgtgctgg cctccgttca ccaccccgcc ctgatcgtgt
13021 tccagtgctg caatcccgtg tacaggaata gcagagctca gggtggaggc cccaactgcg
13081 acttcaagat ctccgcccca gatctgctga acgctctcgt gatggtgcgg agcctgtggt
13141 ctgagaactt cacagagctg ccccgcatgg tggtgcccga gttcaagtgg agcactaagc
13201 atcagtaccg gaatgtgtct ctgcccgtgg cccactctga cgccagacag aatccttttg
13261 acttctgaac ggcgcagacg gcaagggtgg ggggtaaata atcacccgag agtgtacaaa
13321 taaaaacatt tgcctttatt gaaagtgtct cctagtacat tatttttaca tgtttttcaa
13381 gtgacaaaaa gaagtggcgc tcctaatctg cgcactgtgg ctgcggaagt agggcgagtg
13441 gcgctccagg aagctgtaga gctgttcctg gttgcgacgc agggtgggct gtacctgggg
13501 actgttaagc atggagttgg gtaccggatc catcgcgccc tgtagcggcg cattaagcgc
13561 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
13621 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
13681 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
13741 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
13801 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
13861 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
13921 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
13981 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
14041 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
14101 tattgaaaaa ggaagagtat gattgaacaa gatggattgc acgcaggttc tccggccgct
14161 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
14221 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
14281 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
14341 gttccttgcg cagctgtact cgacgttgtc actgaagcgg gaagggactg gctgctattg
14401 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
14461 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
14521 caccaagcga aacatcgcat cgagagagca cgtactcgga tggaagccgg tcttgtcgat
14581 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
14641 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
14701 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
14761 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
14821 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
14881 gccttctatc gccttcttga cgagttcttc tgactgtcag accaagttta ctcatatata
14941 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
15001 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
15061 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
15121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
15181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
15241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
15301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
15361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
15421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
15481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
15541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
15601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
15661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
15721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
15781 tttgagtgag ctgatcatat ggtttaaacg ttggcag
//
pAAVone-AAV-PHP.eB-CMV-eGFP载体图谱质粒图谱pdf版和pAAVone-AAV-PHP.eB-CMV-eGFP载体序列质粒序列等相关资料下载
pAAVone-AAV-PHP.eB-CMV-eGFP质粒载体应用举例
- 上一篇：CMV-Tol2 transposase
- 下一篇：没有了
