pIVT-iCre

LOCUS pIVT-iCre 3369 bp DNA circular SYN 26-MAR-2026 DEFINITION IVT template for making modRNA encoding iCre. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3369) AUTHORS Blanch-Asensio A, Ploessl DS, Wang NB, Mummery CL, Galloway KE, Davis RP TITLE STRAIGHT-IN Dual: a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells. JOURNAL bioRxiv [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 10.1101/2024.10.17.616637. PUBMED 39464154 REFERENCE 2 (bases 1 to 3369) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 26, 2026 from SnapGene Server 8.0.1 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 10.1101/2024.10.17.616637." COMMENT Sequence Label: pIVT-iCre FEATURES Location/Qualifiers source 1..3369 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 22..126 /gene="bla" /label=AmpR promoter CDS join(127..195,196..987) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(345..364) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1158..1746 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1647..1666 /label=pBR322ori-F /note="pBR322 origin, forward primer" regulatory 1818..1827 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 1830..1850 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal join(2996..3369,1..21) /label=beta-globin poly(A) /note="human beta-globin polyadenylation signal" ORIGIN 1 gttttgctat gctgtatttt acgcggaacc cctatttgtt tatttttcta aatacattca 61 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 121 aagagtatga gtattcaaca tttccgtgtc gctcttattc ccttttttgc ggcattttgc 181 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 241 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 301 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 361 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 421 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 481 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 541 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 601 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 661 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 721 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 781 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 841 gggtatcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 901 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 961 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 1021 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 1081 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 1141 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 1201 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 1261 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 1321 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 1381 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 1441 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 1501 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 1561 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 1621 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 1681 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 1741 tggaaactcc tgggcaacgt gctggtctgt gtgctggccc atcactttgg caaagcacgc 1801 taccggtcac gcgtgccgcc accatggtgc ccaagaagaa gaggaaagtc tccaacctgc 1861 tgactgtgca ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga 1921 agaacctgat ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc 1981 tcctgtctgt gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc 2041 ctgctgaacc tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg 2101 tgaagaccat ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc 2161 ctcgcccttc tgactccaat gctgtgtccc tggtgatgag gagaatcaga aaggagaatg 2221 tggatgctgg ggagagagcc aagcaggccc tggcctttga acgcactgac tttgaccaag 2281 tcagatccct gatggagaac tctgacagat gccaggacat caggaacctg gccttcctgg 2341 gcattgccta caacaccctg ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca 2401 tctcccgcac cgatggtggg agaatgctga tccacattgg caggaccaag accctggtgt 2461 ccacagctgg tgtggagaag gccctgtccc tgggggttac caagctggtg gagagatgga 2521 tctctgtgtc tggtgtggct gatgacccca acaactacct gttctgccgg gtcagaaaga 2581 atggtgtggc tgccccttct gccacctccc aactgtccac ccgggccctg gaagggatct 2641 ttgaggccac ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg 2701 cctggtctgg ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt 2761 ccatccctga aatcatgcag gctggtggct ggaccaatgt gaacattgtg atgaactaca 2821 tcagaaacct ggactctgag actggggcca tggtgaggct gctcgaggat ggggactgag 2881 cggccgcact cctcaggtct cgagctcaag cttcgaattc accccaccag tgcaggctgc 2941 ctatcagaaa gtggtggctg gtgtggctaa tgccctggcc cacaagtatc actaagctcg 3001 ctttcttgct gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac 3061 tgggggatat tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt 3121 tcattgcaat gatgtattta aattatttct gaatatttta ctaaaaaggg aatgtgggag 3181 gtcagtgcat ttaaaacata aagaaatgaa gagctagttc aaaccttggg aaaatacact 3241 atatcttaaa ctccatgaaa gaaggtgagg ctgcaaacag ctaatgcaca ttggcaacag 3301 cccctgatgc ctatgcctta ttcatccctc agaaaaggat tcaagtagag gcttgatttg 3361 gaggttaaa //