pBiFC-CC155

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pBiFC-CC155 
出品公司: 爱迪基因
目录编号: 22015
存储实验室: Chang-Deng Hu

载体骨架基本信息
载体名称: pCMV-HA
载体抗性: 氨苄霉素
空载体大小: 3833 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: CC155
物种来源: 维多利亚水母
插入大小: 255 bp
基因突变: N/A
标签/融合蛋白: HA(N端)
克隆方法: 限制性内切酶
5' 克隆位点: KpnI(未破坏)
3' 克隆位点: NotI (未破坏)
5' 测序引物 CMV-F
3' 测序引物 EBV-Rev

文献引用方法
材料和方法部分: pBiFC-CC155 was a gift from Chang-Deng Hu (Addgene plasmid # 22015)
参考文献部分: Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Jan . 40(1):61-6. 10.2144/000112036 PubMed 16454041

载体图谱质粒图谱和多克隆位点信息

pBiFC-CC155载体图谱

载体简介

载体序列

LOCUS       pBiFC-CC155	4088 bp 	DNA	SYN
DEFINITION  pBiFC-CC155
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="pBiFC-CC155"
                     /mol_type="other DNA"
     misc_feature    31..318
                     /label="CAG_enhancer"
     promoter        36..528
                     /label="CMV_immearly_promoter"
     misc_feature    485..505
                     /label="CMV_fwd_primer"
     promoter        486..555
                     /label="CMV_promoter"
     promoter        498..617
                     /label="CMV2_promoter"
     misc_feature    529..548
                     /label="pCEP_fwd_primer"
     misc_feature    531..555
                     /label="LNCX_primer"
     misc_feature    631..657
                     /label="pCMV_primer"
     misc_feature    672..768
                     /label="modSV40_late_16s_int"
     misc_feature    672..702
                     /label="SV40_late_19s_int"
     CDS             750..1286
                     /label="ORF frame 3"
     gene            967..1212
                     /label="ECFP (468 - 713)"
                     /gene="ECFP (468 - 713)"
     misc_feature    1149..1170
                     /label="EGFP_C_primer"
     terminator      complement(1237..1356)
                     /label="SV40_PA_terminator"
     misc_feature    complement(1251..1270)
                     /label="EBV_rev_primer"
     promoter        complement(1468..1486)
                     /label="M13_reverse_primer"
     misc_feature    complement(1485..1507)
                     /label="M13_pUC_rev_primer"
     promoter        complement(1521..1550)
                     /label="lac_promoter"
     gene            complement(2633..3493)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2633..3493)
                     /label="ORF frame 2"
     promoter        complement(3535..3563)
                     /label="AmpR_promoter"
     misc_feature    complement(3722..3744)
                     /label="pGEX_3_primer"
     misc_feature    complement(3943..4086)
                     /label="lacZ_a"
     misc_feature    4057..4079
                     /label="M13_pUC_fwd_primer"
     promoter        4072..4088
                     /label="M13_forward20_primer"
ORIGIN
    1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
   61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
  121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
  181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
  241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
  301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
  361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
  421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
  481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA
  541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC
  601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG
  661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG
  721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA
  781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GTACCCATAC
  841 GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA TTCGGTCGAC CGAGATCTCT
  901 CGAGGTACCC GTCCGGCGTG CAAAATCCCG AACGACCTGA AACAGAAAGT CATGAACCAC
  961 GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCC GCCACAACAT CGAGGACGGC
 1021 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
 1081 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG
 1141 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
 1201 GAGCTGTACA AGTAAGCGGC CGCGGGGATC CAGACATGAT AAGATACATT GATGAGTTTG
 1261 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA
 1321 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC
 1381 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTCGGATCCT CTAGAGTCGA
 1441 TCTGCAGGCA TGCTAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT
 1501 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG
 1561 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG
 1621 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC
 1681 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC
 1741 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA
 1801 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG
 1861 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
 1921 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
 1981 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
 2041 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
 2101 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
 2161 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
 2221 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
 2281 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC
 2341 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG
 2401 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC
 2461 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT
 2521 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA
 2581 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT
 2641 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT
 2701 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG
 2761 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG
 2821 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA
 2881 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG
 2941 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG
 3001 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT
 3061 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA
 3121 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG
 3181 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC
 3241 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG
 3301 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA
 3361 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG
 3421 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT
 3481 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC
 3541 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA
 3601 CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT
 3661 ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA
 3721 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA
 3781 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT
 3841 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA
 3901 GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT
 3961 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG
 4021 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
 4081 CGGCCAGT
//

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