pMETαC

价格:1500元

联系方式:I47-825O-882O

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pMETalpha C载体基本信息

出品公司: Invitrogen
载体名称: pMETalpha C, pMETα C, pMETalphaC, pMETαC
质粒类型: 毕赤酵母蛋白表达载体(methanoloica酵母)
表达水平: 高拷贝
诱导方法: 甲醇诱导
启动子: AUG1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8049 bp
5' 测序引物及序列: AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´;  alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´
3' 测序引物及序列: AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´
载体标签: C-V5, C-His,N-alpha分泌因子
载体抗性: 氨苄
筛选标记: ADE2
备注: 含有alpha factor酵母分泌信号肽,利用甲醇诱导蛋白在methanoloica酵母中分泌表达蛋白。
产品目录号: K1780-01
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

pMETalpha C载体质粒图谱和多克隆位点信息

pMETalpha C载体图谱

pMETalpha C多克隆位点

pMETalpha C载体简介

pMETalpha C载体序列

LOCUS       pMET\alpha\C            8049 bp    DNA     circular      1-AUG-2013
DEFINITION  pMET alpha C.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     http://www.biofeng.com/
COMMENT     VNTNAME|pMET alpha C|
COMMENT     VNTOAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    1106..1130
                     /vntifkey="21"
                     /label=AUG1_fwd_primer
     misc_feature    complement(1668..1689)
                     /vntifkey="21"
                     /label=AUG1_rev_primer
     CDS             3091..3666
                     /vntifkey="4"
                     /label=ORF\frame\1
     CDS             3672..4805
                     /vntifkey="4"
                     /label=ORF\frame\3
     CDS             4969..5967
                     /vntifkey="4"
                     /label=ORF\frame\1
     gene            complement(6780..7640)
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             complement(6780..7640)
                     /vntifkey="4"
                     /label=ORF\frame\2
     promoter        complement(7682..7710)
                     /vntifkey="30"
                     /label=AmpR_promoter
     sig_peptide     1197..1463
                     /vntifkey="94"
                     /label=Alpha\factor\secretion\signal
BASE COUNT     2363 a      1741 c      1568 g      2377 t 
ORIGIN
        1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag 
       61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt 
      121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac 
      181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca 
      241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac 
      301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa 
      361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc 
      421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa 
      481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg 
      541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc 
      601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa 
      661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct 
      721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct 
      781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg 
      841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa 
      901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat 
      961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct 
     1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt 
     1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac 
     1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga 
     1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag 
     1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact 
     1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg 
     1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc 
     1441 tcgagaaaag agaggctgaa gcatcgatga attcacgtgg cccagccggc cgtggatcca 
     1501 cgcgtcgtcg acccgcggcg gccgccagct tactagtagg taagcctatc cctaaccctc 
     1561 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga tctagtatac 
     1621 aattctaggg ctgcctgttt ggatattttt ataatttttg agagtttgcc aactaatgtt 
     1681 tttctcttct atgatattta tcatgtagtt ggatatcttt ttttctttga aaagttttta 
     1741 tttagttctt tctctctttt ttataatgtt ttcagtttct ttcctttata ttgaaaatac 
     1801 gatgttcttt gtctttcttt tttaaatcct caattataat gtgtaccgtt tgatcaactt 
     1861 gtaaaattgt ttgtagtcct gtaatcttaa ttgacgattg gttgatagtt taccccagta 
     1921 tattgtgcgg ctgagatatt acccgattcg gaagattgat tttttcaaca gacataaacc 
     1981 cataggagat ttagtacagc gacatatgct aattgcctga taatgctcac taacattttc 
     2041 tccttttaat ctggtatatg catacaggta tgttgatatg gccttgttga tcaaagctac 
     2101 gaaagtgtag actatttccc aaacagcccc caaccaccac ctcacacgcg cacactgcga 
     2161 agtgaaagcg ctcccttatt ttcttctccg ctggttcaaa acaaaaaaga atctaatctg 
     2221 tagacacacg ccccgtaaaa cccaatcttc atcatcgtaa ttttcagtga cggacgatcg 
     2281 atcaaccata tagtttgtta gtgactgagc ttcagtagtt gacctcttga agaaataacg 
     2341 tcttgaacaa catactttat acctaccaac attcgccatc aacaacagtg tgacactgac 
     2401 ataactttac atccattcat cgacaaaaaa atgtctctag ttcggattca tgcttatggg 
     2461 ttagctattt cgcccaatgt gtccatctga cattactatt ttgcatttta atttaattag 
     2521 aacttgacta gcgcactacc agcatatcat ctcatttccg taaataccaa atgtattata 
     2581 tattgaaagc ttttgaccag gttattataa aagaaacttc atgctcgaaa aagatcattt 
     2641 cgaaaagttg cctagtttca tgaaatttta aagcagttta tataaatttt accttttgat 
     2701 gcggaattga ctttttcttg aataatacat aacttttctt aaaagaatca aagacagata 
     2761 aaatttaaga gatattaaat attagtgaga agccgagaat tttgtaacac caacataaca 
     2821 ctgacatctt taacaacttt taattatgat acatttctta cgtcatgatt gattattaca 
     2881 gctatgctga caaatgactc ttgttgcatg gctacgaacc gggtaatact aagtgattga 
     2941 ctcttgctga ccttttatta agaactaaat ggacaatatt atggagcatt tcatgtataa 
     3001 attggtgcgt aaaatcgttg gatctctctt ctaagtacat cctactataa caatcaagaa 
     3061 aaacaagaaa atcggacaaa acaatcaagt atggattcta gaacagttgg tatattagga 
     3121 gggggacaat tgggacgtat gattgttgag gcagcaaaca ggctcaacat taagacggta 
     3181 atactagatg ctgaaaattc tcctgccaaa caaataagca actccaatga ccacgttaat 
     3241 ggctcctttt ccaatcctct tgatatcgaa aaactagctg aaaaatgtga tgtgctaacg 
     3301 attgagattg agcatgttga tgttcctaca ctaaagaatc ttcaagtaaa acatcccaaa 
     3361 ttaaaaattt acccttctcc agaaacaatc agattgatac aagacaaata tattcaaaaa 
     3421 gagcatttaa tcaaaaatgg tatagcagtt acccaaagtg ttcctgtgga acaagccagt 
     3481 gagacgtccc tattgaatgt tggaagagat ttgggttttc cattcgtctt gaagtcgagg 
     3541 actttggcat acgatggaag aggtaacttc gttgtaaaga ataaggaaat gattccggaa 
     3601 gctttggaag tactgaagga tcgtcctttg tcgccgaaaa atgggcacca tttactaaag 
     3661 aattagcagt catgattgtg agatctgtta acggtttagt gttttcttac ccaattgtag 
     3721 agactatcca caaggacaat atttgtgact tatgttatgc gcctgctaga gttccggact 
     3781 ccgttcaact taaggcgaag ttgttggcag aaaatgcaat caaatctttt cccggttgtg 
     3841 gtatatttgg tgtggaaatg ttctatttag aaacagggga attgcttatt aacgaaattg 
     3901 ccccaaggcc tcacaactct ggacattata ccattgatgc ttgcgtcact tctcaatttg 
     3961 aagctcattt gagatcaata ttggatttgc caatgccaaa gaatttcaca tctttctcca 
     4021 ccattacaac gaacgccatt atgctaaatg ttcttggaga caaacataca aaagataaag 
     4081 agctagaaac ttgcgaaaga gcattggcga ctccaggttc ctcagtgtac ttatatggaa 
     4141 aagagtctag acctaacaga aaagtaggtc acataaatat tattgcctcc agtatggcgg 
     4201 aatgtgaaca aaggctgaac tacattacag gtagaactga tattccaatc aaaatctctg 
     4261 tcgctcaaaa gttggacttg gaagcaatgg tcaaaccatt ggttggaatc atcatgggat 
     4321 cagactctga cttgccggta atgtctgccg catgtgcggt tttaaaagat tttggcgttc 
     4381 catttgaagt gacaatagtc tctgctcata gaactccaca taggatgtca gcatatgcta 
     4441 tttccgcaag caagcgtgga attaaaacaa ttatcgctgg agctggtggg gctgctcact 
     4501 tgccaggtat ggtggctgca atgacaccac ttcctgtcat cggtgtgccc gtaaaaggtt 
     4561 cttgtctaga tggagtagat tctttacatt caattgtgca aatgcctaga ggtgttccag 
     4621 tagctaccgt cgctattaat aatagtacga acgctgcgct gttggctgtc agactgcttg 
     4681 gcgcttatga ttcaagttat acaacgaaaa tggaacagtt tttattaaag caagaagaag 
     4741 aagttcttgt caaagcacaa aagttagaaa ctgtcggtta cgaagcttat ctagaaaaca 
     4801 agtaatatat aagtttattg atatacttgt acagcaaata attataaaat gatataccta 
     4861 ttttttaggc tttgttatga ttacatcaaa tgtggacttc atacatagaa atcaacgctt 
     4921 acaggtgtcc ttttttaaga atttcataca taagatctac tagaatcaat gttaaacctg 
     4981 ttatcattag aacagccatc tccagaaggt tctatccaag tgaaaacttt ggtcttcaag 
     5041 ccaaagactg ctaaaacaca aaccccagtc ccaattattg ttgttgctga aacttctact 
     5101 tctactccgg gtggtgttat agccaaagct tcgggcacta aagagccaag attagcaaac 
     5161 gatgaattga ccaaccaatt ctttggttct cctgctaaag agttttctat tgccagctta 
     5221 tcaaacgaac acaaagacgg taaagtcaag atcattattg atgctgggat caaagatgat 
     5281 gactcgacta attacgttat ttccaacact gttgctaagt tagttttaac cggtaagcaa 
     5341 ttattaggtt tcttatccaa ctacgaacca accatcgttg attttactgc tgaagctgca 
     5401 accgctgccc caactccagt tgttaagaaa gaacctgctg catctggtaa gcttgatgat 
     5461 gccaaattaa ttggtgttac tgctgacaag gaaaaagatt tctccaactg gtactcccaa 
     5521 attctaatga agggtgaaat gttagactac tacgatgtct ctggttgtta cattatgaga 
     5581 ccagcttctt acacaatttg ggaaaagatt caatcttggt tcgattcgaa gattaaagaa 
     5641 gatggtgtta ctaatgctta tttcccaatg tttgtctcat ccaaggtttt agaaaaggaa 
     5701 aaggaccaca tcgatggttt cgctcctgaa gttgcttggg ttaccagagc tggtaaatct 
     5761 gaattagaag aaccaattgc tattagacca acttctgaaa ctgttatggg taccctgcag 
     5821 gggccggcct taattaaggc gcgcccttcc tcgctcactg actcgctgcg ctcggtcgtt 
     5881 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     5941 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     6001 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     6061 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     6121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     6181 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     6241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     6301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     6361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     6421 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 
     6481 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     6541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     6601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     6661 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     6721 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     6781 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     6841 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     6901 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     6961 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     7021 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     7081 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     7141 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     7201 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     7261 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     7321 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     7381 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     7441 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     7501 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     7561 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     7621 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     7681 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     7741 ccgcgcacat ttccccgaaa agtgccacct gtatgcggtg tgaaataccg cacagatgcg 
     7801 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt 
     7861 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 
     7921 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 
     7981 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc aagggcgatg 
     8041 gcccactac 
//

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