| 质粒名称: | HA-HIF1alpha-wt-pBabe-puro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 19365 |
| 存储实验室: | William Kaelin |
|
载体骨架基本信息 |
|
| 载体名称: | pBabe-puro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5188 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Hypoxia inducible factor 1 alpha,HIF1a |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2515 bp |
| 基因突变: | wt human cDNA |
| GenBank编号 | NM_001530 |
| 启动子 | -- |
| 标签/融合蛋白: | HA-tag（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamH I（未销毁） |
| 3' 克隆位点: | Not I（在克隆过程中被破坏） |
| 5' 测序引物 | pBabe-5' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | HA-HIF1alpha-wt-pBabe-puro was a gift from William Kaelin (Addgene plasmid # 19365 ; http://n2t.net/addgene:19365 ; RRID:Addgene_19365) |
| 参考文献部分: | The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Yan Q, Bartz S, Mao M, Li L, Kaelin WG. Mol Cell Biol. 2007 Mar . 27(6):2092-102. 10.1128/MCB.01514-06 PubMed 17220275 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS HA-HIF1alpha-wt-pBabe-puro 7623 bp ds-DNA circular SYN 18-SEP-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS HA-HIF1alpha-wt-pBabe-puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7623)
AUTHORS Yan Q, Bartz S, Mao M, Li L, Kaelin WG
TITLE The hypoxia-inducible factor 2alpha N-terminal and C-terminal
transactivation domains cooperate to promote renal tumorigenesis in
vivo.
JOURNAL Mol Cell Biol. 2007 Mar . 27(6):2092-102.
PUBMED 17220275
REFERENCE 2 (bases 1 to 7623)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 18, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7623
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 530..729
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 930..1346
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1276..1298
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1314..1330
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1378..1404
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
regulatory 1417..1426
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(3941..3961)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3946..4275
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4126..4261
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4188..4207
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4285..4884
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4285..4304)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4781..4801
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 5105..5574
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(5704..5721)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5875..6463)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5955..5974)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6623..7483)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7246..7265
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7484..7588)
/gene="bla"
/label=AmpR promoter
LTR 7619..465
/note="long terminal repeat from Moloney murine leukemia
virus"
ORIGIN
1 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
61 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
121 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
181 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
241 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
301 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
361 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
421 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
481 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca agctggccag
541 caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc
601 ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct
661 gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc
721 cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag
781 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc
841 gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga
901 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
961 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
1021 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
1081 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
1141 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
1201 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
1261 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
1321 cagccctcac tccttctcta ggcgccggcc ggatcgaaga ctaagcttac catggcctac
1381 ccctacgacg tgcccgacta cgcctccctc ggatccgcca ccatggaggg cgccggcggc
1441 gcgaacgaca agaaaaagat aagttctgaa cgtcgaaaag aaaagtctcg agatgcagcc
1501 agatctcggc gaagtaaaga atctgaagtt ttttatgagc ttgctcatca gttgccactt
1561 ccacataatg tgagttcgca tcttgataag gcctctgtga tgaggcttac catcagctat
1621 ttgcgtgtga ggaaacttct ggatgctggt gatttggata ttgaagatga catgaaagca
1681 cagatgaatt gcttttattt gaaagccttg gatggttttg ttatggttct cacagatgat
1741 ggtgacatga tttacatttc tgataatgtg aacaaataca tgggattaac tcagtttgaa
1801 ctaactggac acagtgtgtt tgattttact catccatgtg accatgagga aatgagagaa
1861 atgcttacac acagaaatgg ccttgtgaaa aagggtaaag aacaaaacac acagcgaagc
1921 ttttttctca gaatgaagtg taccctaact agccgaggaa gaactatgaa cataaagtct
1981 gcaacatgga aggtattgca ctgcacaggc cacattcacg tatatgatac caacagtaac
2041 caacctcagt gtgggtataa gaaaccacct atgacctgct tggtgctgat ttgtgaaccc
2101 attcctcacc catcaaatat tgaaattcct ttagatagca agactttcct cagtcgacac
2161 agcctggata tgaaattttc ttattgtgat gaaagaatta ccgaattgat gggatatgag
2221 ccagaagaac ttttaggccg ctcaatttat gaatattatc atgctttgga ctctgatcat
2281 ctgaccaaaa ctcatcatga tatgtttact aaaggacaag tcaccacagg acagtacagg
2341 atgcttgcca aaagaggtgg atatgtctgg gttgaaactc aagcaactgt catatataac
2401 accaagaatt ctcaaccaca gtgcattgta tgtgtgaatt acgttgtgag tggtattatt
2461 cagcacgact tgattttctc ccttcaacaa acagaatgtg tccttaaacc ggttgaatct
2521 tcagatatga aaatgactca gctattcacc aaagttgaat cagaagatac aagtagcctc
2581 tttgacaaac ttaagaagga acctgatgct ttaactttgc tggccccagc cgctggagac
2641 acaatcatat ctttagattt tggcagcaac gacacagaaa ctgatgacca gcaacttgag
2701 gaagtaccat tatataatga tgtaatgctc ccctcaccca acgaaaaatt acagaatata
2761 aatttggcaa tgtctccatt acccaccgct gaaacgccaa agccacttcg aagtagtgct
2821 gaccctgcac tcaatcaaga agttgcatta aaattagaac caaatccaga gtcactggaa
2881 ctttctttta ccatgcccca gattcaggat cagacaccta gtccttccga tggaagcact
2941 agacaaagtt cacctgagcc taatagtccc agtgaatatt gtttttatgt ggatagtgat
3001 atggtcaatg aattcaagtt ggaattggta gaaaaacttt ttgctgaaga cacagaagca
3061 aagaacccat tttctactca ggacacagat ttagacttgg agatgttagc tccctatatc
3121 ccaatggatg atgacttcca gttacgttcc ttcgatcagt tgtcaccatt agaaagcagt
3181 tccgcaagcc ctgaaagcgc aagtcctcaa agcacagtta cagtattcca gcagactcaa
3241 atacaagaac ctactgctaa tgccaccact accactgcca ccactgatga attaaaaaca
3301 gtgacaaaag accgtatgga agacattaaa atattgattg catctccatc tcctacccac
3361 atacataaag aaactactag tgccacatca tcaccatata gagatactca aagtcggaca
3421 gcctcaccaa acagagcagg aaaaggagtc atagaacaga cagaaaaatc tcatccaaga
3481 agccctaacg tgttatctgt cgctttgagt caaagaacta cagttcctga ggaagaacta
3541 aatccaaaga tactagcttt gcagaatgct cagagaaagc gaaaaatgga acatgatggt
3601 tcactttttc aagcagtagg aattggaaca ttattacagc agccagacga tcatgcagct
3661 actacatcac tttcttggaa acgtgtaaaa ggatgcaaat ctagtgaaca gaatggaatg
3721 gagcaaaaga caattatttt aataccctct gatttagcat gtagactgct ggggcaatca
3781 atggatgaaa gtggattacc acagctgacc agttatgatt gtgaagttaa tgctcctata
3841 caaggcagca gaaacctact gcagggtgaa gaattactca gagctttgga tcaagttaac
3901 tgacaattct gcagatatcc atcacagtgg tcgaccctgt ggaatgtgtg tcagttaggg
3961 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
4021 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
4081 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
4141 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
4201 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
4261 ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
4321 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
4381 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
4441 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
4501 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
4561 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
4621 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
4681 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
4741 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
4801 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
4861 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
4921 agcgcacgac cccatgcatc gataaaataa aagattttat ttagtctcca gaaaaagggg
4981 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
5041 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga
5101 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
5161 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
5221 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
5281 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
5341 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
5401 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
5461 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
5521 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca
5581 gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt
5641 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
5701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
5761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
5821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
5881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
5941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
6001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
6061 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
6121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
6181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
6241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
6301 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
6361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
6421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
6481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
6541 attatcaaaa aggatcttca cctagatcct tttgcggccg caaatcaatc taaagtatat
6601 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
6661 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
6721 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
6781 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
6841 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
6901 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
6961 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
7021 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
7081 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
7141 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
7201 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
7261 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
7321 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
7381 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
7441 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
7501 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
7561 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcagcct
7621 gaa
//
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