pCMV-StayGold-iPAK4

LOCUS       pCMV-StayGold-iPAK4            6601 bp DNA     circular SYN 23-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS
  TITLE     Cerulean, Venus, and VenusY67C FRET reference standards.
  JOURNAL   Biophys J. 2006 Dec 15. 91(12):L99-L101.
  PUBMED    17040988
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys J. 
            2006 Dec 15. 91(12):L99-L101."
FEATURES             Location/Qualifiers
     source          1..6601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1330..2406
                     /mol_type="genomic DNA"
                     /organism="."
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    591..671
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             679..1329
                     /label=StayGold
     primer_bind     1329..1355
                     /label=GFP-iPAK4-F
     primer_bind     1329..1355
                     /label=Primer 1a
     CDS             1342..1455
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             1462..2403
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(1555..1573)
                     /label=PAK4-R
     primer_bind     complement(2385..2425)
                     /label=NotI-PAK4-R
     polyA_signal    2528..2649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2565..2584)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2619..2638
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2656..3111)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2793..2814)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3005..3024
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3138..3242
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3226..3270
                     /label=Primer 1
     CDS             3243..4103
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4077..4121)
                     /label=Primer 3
     promoter        4105..4462
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(4128..4148)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     4308..4320
                     /label=mCherry-Flag-R
     rep_origin      4313..4448
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4375..4394
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4398..4409)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4497..5291
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4551..4570)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5161..5180
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5479..5498)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5523..5570
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5899..6487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6388..6407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaccgg tcgccaccat ggccagcacc cccttcaagt tccagctgaa gggcaccatc
      721 aacggcaaga gcttcaccgt ggagggcgag ggcgagggca acagccacga gggcagccac
      781 aagggcaagt acgtgtgcac cagcggcaag ctgcccatga gctgggccgc cctgggcacc
      841 agcttcggct acggcatgaa gtactacacc aagtacccca gcggcctgaa gaactggttc
      901 cacgaggtga tgcccgaggg cttcacctac gacaggcaca tccagtacaa gggcgacggc
      961 agcatccacg ccaagcacca gcacttcatg aagaacggca cctaccacaa catcgtggag
     1021 ttcaccggcc aggacttcaa ggagaacagc cccgtgctga ccggcgacat gaacgtgagc
     1081 ctgcccaacg aggtgcagca catccccagg gacgacggcg tggagtgccc cgtgaccctg
     1141 ctgtaccccc tgctgagcga caagagcaag tgcgtggagg cccaccagaa caccatctgc
     1201 aagcccctgc acaaccagcc cgcccccgac gtgccctacc actggatcag gaagcagtac
     1261 acccagagca aggacgacac cgaggagagg gaccacatct gccagagcga gaccctggag
     1321 gcccacctgt caggtggatc cgaagcagag gactggacag cagccctact gaacaggggt
     1381 cgcagtcgcc agcccctggt actaggggac aattgctttg ctgacttggt gcacaactgg
     1441 atggagctgc ctgaggaatt ccccgccgcc cctgctgttc ctgggccccc tggcccccgc
     1501 tcaccacagc gggagccaca gcgagtatcc catgagcagt tccgggctgc cctgcagctg
     1561 gtggtggacc caggcgaccc ccgctcctac ctggacaact tcatcaagat tggcgagggc
     1621 tccacgggca tcgtgtgcat cgccaccgtg cgcagctcgg gcaagctggt ggccgtcaag
     1681 aagatggacc tgcgcaagca gcagaggcgc gagctgctct tcaacgaggt ggtaatcatg
     1741 agggactacc agcacgagaa tgtggtggag atgtacaaca gctacctggt gggggacgag
     1801 ctctgggtgg tcatggagtt cctggaagga ggcgccctca ccgacatcgt cacccacacc
     1861 aggatgaacg aggagcagat cgcagccgtg tgccttgcag tgctgcaggc cctgtcggtg
     1921 ctccacgccc agggcgtcat ccaccgggac atcaagagcg actcgatcct gctgacccat
     1981 gatggcaggg tgaagctgtc agactttggg ttctgcgccc aggtgagcaa ggaagtgccc
     2041 cgaaggaagt cgctggtcgg cacgccctac tggatggccc cagagctcat ctcccgcctt
     2101 ccctacgggc cagaggtaga catctggtcg ctggggataa tggtgattga gatggtggac
     2161 ggagagcccc cctacttcaa cgagccaccc ctcaaagcca tgaagatgat tcgggacaac
     2221 ctgccacccc gactgaagaa cctgcacaag gtgtcgccat ccctgaaggg cttcctggac
     2281 cgcctgctgg tgcgagaccc tgcccagcgg gccacggcag ccgagctgct gaagcaccca
     2341 ttcctggcca aggcagggcc gcctgccagc atcgtgcccc tcatgcgcca gaaccgcacc
     2401 agatgaagcg gccgcgactc tagacataat cagccatacc acatttgtag aggttttact
     2461 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2521 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2581 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2641 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2701 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2761 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2821 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2881 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2941 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3001 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3061 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     3121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3181 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3241 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3301 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3361 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3421 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3481 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3541 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3601 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3661 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3721 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3781 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3841 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3901 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct
     3961 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4021 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4081 cctcactgat taagcattgg taacctgagg cggaaagaac cagctgtgga atgtgtgtca
     4141 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4201 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4261 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4321 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4381 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4441 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     4501 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     4561 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     4621 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     4681 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4741 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4801 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4861 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4921 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4981 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     5041 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5101 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5161 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5221 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5281 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5341 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5401 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5461 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5521 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     5581 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     5641 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     5701 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     5761 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5821 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5881 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5941 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6001 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6061 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6301 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6541 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     6601 t
//