pcDNA3-TLR9-YFP

LOCUS       pcDNA3-TLR9-YFP              9198 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3-TLR9-YFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9198)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 9198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2215..2224
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4015..4734
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4060..4081)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4321..4340)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4668..4689
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4750..4768)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4751..4768)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4788..4805)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4794..5018
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5064..5492
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5151..5170)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5361..5382
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5501..5521)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5506..5836
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5687..5822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5749..5768
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5903..6697
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5957..5976)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6567..6586
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6871..6992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6908..6927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6962..6981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7041..7057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7041..7057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7054..7076)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7065..7081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7089..7119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7134..7155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7272..7289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7443..8031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7523..7542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8202..9062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8825..8844
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9063..9167)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccatgggttt ctgccgcagc gccctgcacc cgctgtctct cctggtgcag
      961 gccatcatgc tggccatgac cctggccctg ggtaccttgc ctgccttcct accctgtgag
     1021 ctccagcccc acggcctggt gaactgcaac tggctgttcc tgaagtctgt gccccacttc
     1081 tccatggcag caccccgtgg caatgtcacc agcctttcct tgtcctccaa ccgcatccac
     1141 cacctccatg attctgactt tgcccacctg cccagcctgc ggcatctcaa cctcaagtgg
     1201 aactgcccgc cggttggcct cagccccatg cacttcccct gccacatgac catcgagccc
     1261 agcaccttct tggctgtgcc caccctggaa gagctaaacc tgagctacaa caacatcatg
     1321 actgtgcctg cgctgcccaa atccctcata tccctgtccc tcagccatac caacatcctg
     1381 atgctagact ctgccagcct cgccggcctg catgccctgc gcttcctatt catggacggc
     1441 aactgttatt acaagaaccc ctgcaggcag gcactggagg tggccccggg tgccctcctt
     1501 ggcctgggca acctcaccca cctgtcactc aagtacaaca acctcaccgt ggtgccccgc
     1561 aacctgcctt ccagcctgga gtatctgctg ttgtcctaca atcgcatcgt caaactggcg
     1621 cctgaggacc tggccaatct gaccgccctg cgtgtgctcg atgtgggcgg aaattgccgc
     1681 cgctgcgacc acgctcccaa cccctgcatg gagtgccctc gtcacttccc ccagctacat
     1741 cccgatacct tcagccacct gagccgtctt gaaggcctgg tgttgaagga cagttctctc
     1801 tcctggctga atgccagttg gttccgtggg ctgggaaacc tccgagtgct ggacctgagt
     1861 gagaacttcc tctacaaatg catcactaaa accaaggcct tccagggcct aacacagctg
     1921 cgcaagctta acctgtcctt caattaccaa aagagggtgt cctttgccca cctgtctctg
     1981 gccccttcct tcgggagcct ggtcgccctg aaggagctgg acatgcacgg catcttcttc
     2041 cgctcactcg atgagaccac gctccggcca ctggcccgcc tgcccatgct ccagactctg
     2101 cgtctgcaga tgaacttcat caaccaggcc cagctcggca tcttcagggc cttccctggc
     2161 ctgcgctacg tggacctgtc agacaaccgc atcagcggag cttcggagct gacagccacc
     2221 atgggggagg cagatggagg ggagaaggtc tggctgcagc ctggggacct tgctccggcc
     2281 ccagtggaca ctcccagctc tgaagacttc aggcccaact gcagcaccct caacttcacc
     2341 ttggatctgt cacggaacaa cctggtgacc gtgcagccgg agatgtttgc ccagctctcg
     2401 cacctgcagt gcctgcgcct gagccacaac tgcatctcgc aggcagtcaa tggctcccag
     2461 ttcctgccgc tgaccggtct gcaggtgcta gacctgtccc acaataagct ggacctctac
     2521 cacgagcact cattcacgga gctaccacga ctggaggccc tggacctcag ctacaacagc
     2581 cagccctttg gcatgcaggg cgtgggccac aacttcagct tcgtggctca cctgcgcacc
     2641 ctgcgccacc tcagcctggc ccacaacaac atccacagcc aagtgtccca gcagctctgc
     2701 agtacgtcgc tgcgggccct ggacttcagc ggcaatgcac tgggccatat gtgggccgag
     2761 ggagacctct atctgcactt cttccaaggc ctgagcggtt tgatctggct ggacttgtcc
     2821 cagaaccgcc tgcacaccct cctgccccaa accctgcgca acctccccaa gagcctacag
     2881 gtgctgcgtc tccgtgacaa ttacctggcc ttctttaagt ggtggagcct ccacttcctg
     2941 cccaaactgg aagtcctcga cctggcagga aaccagctga aggccctgac caatggcagc
     3001 ctgcctgctg gcacccggct ccggaggctg gatgtcagct gcaacagcat cagcttcgtg
     3061 gcccccggct tcttttccaa ggccaaggag ctgcgagagc tcaaccttag cgccaacgcc
     3121 ctcaagacag tggaccactc ctggtttggg cccctggcga gtgccctgca aatactagat
     3181 gtaagcgcca accctctgca ctgcgcctgt ggggcggcct ttatggactt cctgctggag
     3241 gtgcaggctg ccgtgcccgg tctgcccagc cgggtgaagt gtggcagtcc gggccagctc
     3301 cagggcctca gcatctttgc acaggacctg cgcctctgcc tggatgaggc cctctcctgg
     3361 gactgtttcg ccctctcgct gctggctgtg gctctgggcc tgggtgtgcc catgctgcat
     3421 cacctctgtg gctgggacct ctggtactgc ttccacctgt gcctggcctg gcttccctgg
     3481 cgggggcggc aaagtgggcg agatgaggat gccctgccct acgatgcctt cgtggtcttc
     3541 gacaaaacgc agagcgcagt ggcagactgg gtgtacaacg agcttcgggg gcagctggag
     3601 gagtgccgtg ggcgctgggc actccgcctg tgcctggagg aacgcgactg gctgcctggc
     3661 aaaaccctct ttgagaacct gtgggcctcg gtctatggca gccgcaagac gctgtttgtg
     3721 ctggcccaca cggaccgggt cagtggtctc ttgcgcgcca gcttcctgct ggcccagcag
     3781 cgcctgctgg aggaccgcaa ggacgtcgtg gtgctggtga tcctgagccc tgacggccgc
     3841 cgctcccgct acgtgcggct gcgccagcgc ctctgccgcc agagtgtcct cctctggccc
     3901 caccagccca gtggtcagcg cagcttctgg gcccagctgg gcatggccct gaccagggac
     3961 aaccaccact tctataaccg gaacttctgc cagggaccca cggccgaact cgagatggtg
     4021 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4081 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4141 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4201 accaccttcg gctacggcct gaagtgcttc gcccgctacc ccgaccacat gaagcagcac
     4261 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4321 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4381 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4441 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     4501 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     4561 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4621 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4681 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaatctaga
     4741 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
     4801 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4861 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4921 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4981 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5041 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5101 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5161 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5221 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5281 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5341 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5401 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
     5461 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     5521 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta
     5581 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5641 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5701 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5761 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5821 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5881 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5941 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     6001 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     6061 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     6121 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     6181 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     6241 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     6301 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     6361 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6421 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6481 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6541 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6601 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6661 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6721 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6781 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6841 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6901 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6961 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     7021 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7081 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7141 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7201 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7261 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7321 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7381 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7441 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7501 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7561 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7621 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7681 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7741 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7801 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7861 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7921 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7981 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8041 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8101 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8161 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8221 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8281 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8341 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8401 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8461 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8521 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8581 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8641 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8701 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8761 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8821 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8881 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8941 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9001 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9061 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9181 aaagtgccac ctgacgtc
//