PX393

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: PX393 或 PX393 Gluconacetobacter diazotrophicus Cas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 10346 bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
载体标签: NLS (C ter);HA、NLS (N ter)
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3
宿主细胞(系): 昆虫细胞
备注: --
产品目录号: #68330
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

PX393载体图谱



载体简介

载体序列

LOCUS       PX393 Gluconacetobacter diazotrophicus Cas9	10346 bp 	DNA	SYN
DEFINITION  PX393 Gluconacetobacter diazotrophicus Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="PX393 Gluconacetobacter diazotrophicus Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5037
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMGENMIDESLTFGIDLGIGSCGW
                     AVLRRPSAFGRKGVIEGMGSWCFDVPETSKERTPTNQIRRSNRLLRRVIRRRRNRMAA
                     IRRLLHAAGLLPSTDSDALKRPGHDPWELRARGLDKPLKPVEFAVVLGHIAKRRGFKS
                     AAKRKATNISSDDKKMLTALEATRERLGRYRTVGEMFARDPDFASRRRNREGKYDRTT
                     ARDDLEHEVHALFAAQRRLGQGFASPELEEAFTASAFHQRPMQDSERLVGFCPFERTE
                     KRAAKLTPSFERFRLLARLLNLRITTPDGERPLTVDEIALVTRDLGKTAKLSIKRVRT
                     LIGLEDNQRFTTIRPEDEDRDIVARTGGAMTGTATLRKALGEALWTDMQERPEQLDAI
                     VQVLSFFEANETITEKLREIGLTLAVLDVLLTALDAGVFAKFKGAAHISTKAARNLLP
                     HLEQGRRYDEACTMAGYDHAASRLSHHGQIVAKTQFNALVTEIGESIANPIARKALIE
                     GLKQIWAMRNHWGLPGSIHVELARDVGNSIEKRREIEKHIEKNTALRARERREVHDLL
                     DLEDVNGDTLLRYRLWKEQGGKCLYTGKAIHIRQIAATDNSVQVDHILPWSRFGDDSF
                     NNKTLCLASANQQKKRSTPYEWLSGQTGDAWNAFVQRIETNKELRGFKKRNYLLKNAK
                     EAEEKFRSRNLNDTRYAARLFAEAVKLLYAFGERQEKGGNRRVFTRPGALTAALRQAW
                     GVESLKKQDGKRINDDRHHALDALTVAAVDEAEIQRLTKSFHEWEQQGLGRPLRRVEP
                     PWESFRADVEATYPEVFVARPERRRARGEGHAATIRQVKERECTPIVFERKAVSSLKE
                     ADLERIKDGERNEAIVEAIRSWIATGRPADAPPRSPRGDIITKIRLATTIKAAVPVRG
                     GTAGRGEMVRADVFSKPNRRGKDEWYLVPVYPHQIMNRKAWPKPPMRSIVANKDEDEW
                     TEVGPEHQFRFSLYPRSNIEIIRPSGEVIEGYFVGLHRNTGALTISAHNDPKSIHSGI
                     GTKTLLAISKYQVDRFGRKSPVRKEVRTWHGEACISPTPPG*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMGENMIDESLTFGIDLGIGSCGW
                     AVLRRPSAFGRKGVIEGMGSWCFDVPETSKERTPTNQIRRSNRLLRRVIRRRRNRMAA
                     IRRLLHAAGLLPSTDSDALKRPGHDPWELRARGLDKPLKPVEFAVVLGHIAKRRGFKS
                     AAKRKATNISSDDKKMLTALEATRERLGRYRTVGEMFARDPDFASRRRNREGKYDRTT
                     ARDDLEHEVHALFAAQRRLGQGFASPELEEAFTASAFHQRPMQDSERLVGFCPFERTE
                     KRAAKLTPSFERFRLLARLLNLRITTPDGERPLTVDEIALVTRDLGKTAKLSIKRVRT
                     LIGLEDNQRFTTIRPEDEDRDIVARTGGAMTGTATLRKALGEALWTDMQERPEQLDAI
                     VQVLSFFEANETITEKLREIGLTLAVLDVLLTALDAGVFAKFKGAAHISTKAARNLLP
                     HLEQGRRYDEACTMAGYDHAASRLSHHGQIVAKTQFNALVTEIGESIANPIARKALIE
                     GLKQIWAMRNHWGLPGSIHVELARDVGNSIEKRREIEKHIEKNTALRARERREVHDLL
                     DLEDVNGDTLLRYRLWKEQGGKCLYTGKAIHIRQIAATDNSVQVDHILPWSRFGDDSF
                     NNKTLCLASANQQKKRSTPYEWLSGQTGDAWNAFVQRIETNKELRGFKKRNYLLKNAK
                     EAEEKFRSRNLNDTRYAARLFAEAVKLLYAFGERQEKGGNRRVFTRPGALTAALRQAW
                     GVESLKKQDGKRINDDRHHALDALTVAAVDEAEIQRLTKSFHEWEQQGLGRPLRRVEP
                     PWESFRADVEATYPEVFVARPERRRARGEGHAATIRQVKERECTPIVFERKAVSSLKE
                     ADLERIKDGERNEAIVEAIRSWIATGRPADAPPRSPRGDIITKIRLATTIKAAVPVRG
                     GTAGRGEMVRADVFSKPNRRGKDEWYLVPVYPHQIMNRKAWPKPPMRSIVANKDEDEW
                     TEVGPEHQFRFSLYPRSNIEIIRPSGEVIEGYFVGLHRNTGALTISAHNDPKSIHSGI
                     GTKTLLAISKYQVDRFGRKSPVRKEVRTWHGEACISPTPPG*"
     CDS             complement(1845..2327)
                     /label="ORF frame 1"
                     /translation="MFVAFLFAADLNPLRFAMWPRTTANSTGFSGLSRPRALSSQGSC
                     PGRFRASLSVDGSRPAACSSRRIAAIRFLLRRITRLSSLLDLRIWLVGVRSLDVSGTS
                     KHQEPIPSITPFRPKALGRLSTAHPQLPIPRSMPKVRLSSIIFSPMDASTLRFFFGEA
                     *"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MFVAFLFAADLNPLRFAMWPRTTANSTGFSGLSRPRALSSQGSC
                     PGRFRASLSVDGSRPAACSSRRIAAIRFLLRRITRLSSLLDLRIWLVGVRSLDVSGTS
                     KHQEPIPSITPFRPKALGRLSTAHPQLPIPRSMPKVRLSSIIFSPMDASTLRFFFGEA
                     *"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MFVAFLFAADLNPLRFAMWPRTTANSTGFSGLSRPRALSSQGSC
                     PGRFRASLSVDGSRPAACSSRRIAAIRFLLRRITRLSSLLDLRIWLVGVRSLDVSGTS
                     KHQEPIPSITPFRPKALGRLSTAHPQLPIPRSMPKVRLSSIIFSPMDASTLRFFFGEA
                     *"
     CDS             complement(2578..3402)
                     /label="ORF frame 3"
                     /translation="MDTSGQPPVVSHGPNLFQPLDQCLPSDWIGNGFADLSDQGVELC
                     LCHDLAMVRQAGGCVVIPCHCAGLIVAPALLQMWQQIPSGFGADVCSAFKLGEHSGIQ
                     CGQQDVQHGQSQANFPQLLSDCFVGLKEAQDLHDSVQLFRALLHISPQGLSQCLPQGG
                     SPCHCPACPSHNVSIFVLGADSCEPLIVLQSNQSSHPLDTQFCGLAQVPGDQSNFIHC
                     QGPLSVWGSDSQVQQPGQQSKALERGCQFGCPLFGSLKRAESHQALAVLHGPLMKC*"
     CDS             complement(4251..4934)
                     /label="ORF frame 1"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     misc_feature    complement(5070..5087)
                     /label="Sp6_primer"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     misc_feature    5092..5116
                     /label="attB2"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     terminator      5128..5594
                     /label="hGH_PA_terminator"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     terminator      5600..5635
                     /label="T7_terminator"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     rep_origin      5752..6058
                     /label="f1_origin"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     misc_feature    complement(6172..6192)
                     /label="pBABE_3_primer"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     misc_feature    complement(6178..6393)
                     /label="SV40_enhancer"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     promoter        6190..6458
                     /label="SV40_promoter"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     rep_origin      6357..6434
                     /label="SV40_origin"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     misc_feature    6419..6438
                     /label="SV40pro_F_primer"
                     /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                     MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                     FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                     QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                     SGRATNTSG*"
     CDS             6573..7367
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6576..7364
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(6882..7418)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7544..7663
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7632..7651
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7854..7889
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(7912..7929)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7919..7996
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8026..8643)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    8623..8656
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9424..10284)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9424..10284)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatggga gagaatatga ttgacgagag tctgaccttc
 1921 ggcattgacc tggggattgg gagttgtggg tgggcagtgc tgaggcggcc aagcgccttc
 1981 ggacggaagg gcgtgatcga gggaatgggc tcctggtgct ttgacgtccc cgagacatct
 2041 aaagaacgga ctcctaccaa ccagatccgg agatccaata gactgctgag gcgcgtgatc
 2101 cgacggagaa ggaaccggat ggccgctatt cgccgactgc tgcacgcagc aggcctgctg
 2161 ccatcaaccg acagcgatgc cctgaagcgg cccggacatg atccttggga actgagggca
 2221 cgaggcctgg acaagccact gaaacccgtg gagttcgctg tggtcctggg ccacatcgca
 2281 aagcggagag ggtttaaatc cgctgcaaag agaaaagcca caaacattag ctccgacgat
 2341 aagaaaatgc tgacagccct ggaggctact cgagaacggc tggggagata caggaccgtg
 2401 ggagaaatgt tcgccaggga ccctgatttt gcttctaggc gccgaaatcg cgagggcaag
 2461 tatgatagga ccacagctcg cgacgatctg gagcacgaag tgcacgccct gttcgcagct
 2521 cagcggagac tgggacaggg atttgccagt ccagaactgg aggaggcctt caccgcttca
 2581 gcatttcatc agaggcccat gcaggacagc gagcgcctgg tgggattctg cccttttgag
 2641 cgaaccgaaa agcgggcagc caaactgaca ccctctttcg agcgctttcg actgctggcc
 2701 cggctgctga acctgagaat cactacccca gacggagagc ggcccctgac agtggatgaa
 2761 attgctctgg tcacccggga cctgggcaag accgcaaaac tgagtatcaa gcgggtgaga
 2821 actctgattg gactggagga caatcagagg ttcacaacta tccgccccga ggacgaagat
 2881 cgagacattg tggctcggac aggcggggca atgacaggga ctgccaccct gaggaaggca
 2941 ctgggagagg ccctgtggac tgatatgcag gagcgccctg aacagctgga cgctatcgtg
 3001 caggtcctga gcttctttga ggccaacgaa acaatcactg agaagctgag ggaaattggc
 3061 ctgactctgg ccgtgctgga cgtcctgctg accgcactgg atgccggagt gttcgccaag
 3121 tttaaaggcg ctgcacacat cagcaccaaa gccgctagga atctgctgcc acatctggag
 3181 cagggcaggc gctacgatga ggcctgcaca atggcagggt atgaccacgc agcctcccgc
 3241 ctgtctcacc atggccagat cgtggcaaag acacagttca acgccctggt cactgagatc
 3301 ggcgaatcca ttgccaatcc aatcgctcgg aaggcactga tcgaggggct gaaacagatt
 3361 tgggccatga gaaaccactg ggggctgccc ggaagtatcc atgtggagct ggcccgggat
 3421 gtcggcaact caattgaaaa gcgacgggag attgaaaagc acatcgagaa aaatactgcc
 3481 ctgagggctc gcgagagaag ggaggtgcat gatctgctgg acctggaaga tgtcaatggc
 3541 gacaccctgc tgcgataccg gctgtggaag gagcagggag gcaaatgcct gtatacaggg
 3601 aaggccatcc acattcggca gatcgctgca actgacaact ccgtgcaggt cgatcatatt
 3661 ctgccttgga gccggttcgg cgacgatagt tttaacaaca agaccctgtg tctggcctct
 3721 gctaatcagc agaagaaaag gtcaacacca tacgagtggc tgagcggcca gactggggat
 3781 gcatggaacg ccttcgtgca gcgcatcgag acaaataagg aactgagagg gtttaagaaa
 3841 aggaactatc tgctgaagaa tgctaaagag gcagaggaaa aattcagaag caggaacctg
 3901 aatgacacca gatacgccgc taggctgttc gcagaggccg tgaagctgct gtatgccttt
 3961 ggggagagac aggaaaaagg gggaaaccgc cgagtgttta ctcggcctgg agcactgacc
 4021 gcagcactga gacaggcttg gggagtggag agcctgaaga aacaggatgg gaagcgcatc
 4081 aatgacgatc gacaccatgc cctggatgct ctgaccgtgg ctgcagtcga cgaggccgaa
 4141 attcagaggc tgacaaaatc attccacgag tgggaacagc agggcctggg gcggcctctg
 4201 cggagagtgg agccaccttg ggagagcttc cgggcagacg tcgaggctac ctaccctgaa
 4261 gtgtttgtcg cacggccaga gaggcgccga gcaagaggag aaggccatgc cgctaccatc
 4321 cggcaggtga aggagagaga atgcacacca attgtgtttg agagaaaggc tgtctctagt
 4381 ctgaaagagg cagacctgga acgaatcaaa gatggcgagc gcaacgaagc aattgtggag
 4441 gccatcagga gctggattgc cactggacgc ccagctgatg caccaccacg ctccccccga
 4501 ggcgacatca ttaccaagat caggctggcc accaccatca aggcagccgt gcctgtccgc
 4561 ggagggaccg caggaagggg agaaatggtg cgcgcagatg tgttcagcaa gccaaaccgg
 4621 agagggaaag acgagtggta tctggtgccc gtgtatccac accagatcat gaacaggaag
 4681 gcttggccca aacctccaat gcgctcaatt gtggccaata aggatgagga cgaatggacc
 4741 gaagtgggac ctgaacacca gttccggttt agcctgtacc ctagatccaa tatcgagatc
 4801 attaggccat ctggagaagt gatcgaagga tatttcgtcg gcctgcatcg caacactggc
 4861 gctctgacca tcagtgcaca caatgatccc aagagtatcc attcaggcat tgggaccaag
 4921 acactgctgg ccatttccaa ataccaggtg gacagattcg gcagaaagtc tccagtgcgc
 4981 aaagaggtcc gaacttggca cggggaagcc tgtatctctc ccaccccccc tggataagaa
 5041 ttcggtaccg agctcaagct tgcggccgcc tatagtgtca cctaaatcgt aacccagctt
 5101 tcttgtacaa agtggtcggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
 5161 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
 5221 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
 5281 ggcaagttgg gatgacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
 5341 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
 5401 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
 5461 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
 5521 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
 5581 ccttccctgt ccttctagca taaccccttg gggcctctaa acgggtcttg aggggttttt
 5641 tgtctagaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
 5701 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
 5761 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
 5821 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
 5881 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
 5941 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
 6001 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
 6061 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
 6121 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
 6181 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
 6241 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
 6301 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
 6361 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
 6421 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
 6481 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
 6541 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
 6601 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
 6661 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
 6721 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
 6781 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
 6841 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
 6901 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
 6961 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
 7021 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
 7081 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
 7141 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
 7201 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
 7261 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
 7321 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
 7381 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
 7441 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
 7501 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
 7561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
 7621 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gttcatgaag
 7681 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
 7741 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
 7801 cagaatgagt atttggttta gagtttggca acatatgccc atatgtaact agcataaccc
 7861 cttggggcct ctaaacgggt cttgaggggt tttttgctga aagcatgcgg aggaaattct
 7921 ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac
 7981 tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc
 8041 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
 8101 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
 8161 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
 8221 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
 8281 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
 8341 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
 8401 agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg
 8461 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
 8521 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
 8581 cgcgatcaaa tttgtagttg agctttttgg aattgcgatc gcataacttc gtatagcata
 8641 cattatacga agttataagc tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt
 8701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
 8761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
 8821 gtttttccat aggctccgcc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
 8881 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
 8941 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
 9001 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
 9061 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
 9121 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
 9181 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
 9241 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
 9301 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
 9361 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
 9421 ttgttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
 9481 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
 9541 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
 9601 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
 9661 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
 9721 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
 9781 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
 9841 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
 9901 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
 9961 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
10021 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
10081 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
10141 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
10201 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
10261 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
10321 gggttattgt ctcatgtccg cgcgtt
//

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