pCgCas9_recT

LOCUS       pCgCas9_recT               11170 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Double-plasmid-based CRISPR-Cas9 system in Corynebacterium 
            glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli 
            PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; 
            Kanr.
ACCESSION   .
VERSION     .
KEYWORDS    pCgCas9_recT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Zhang J, Qian F, Dong F, Wang Q, Yang J, Jiang Y, Yang S
  TITLE     De Novo Engineering of Corynebacterium glutamicum for l-Proline 
            Production.
  JOURNAL   ACS Synth Biol. 2020 Jul 17;9(7):1897-1906. doi: 
            10.1021/acssynbio.0c00249. Epub 2020 Jul 6.
  PUBMED    32627539
REFERENCE   2  (bases 1 to 11170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      2694..2916
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2964..3914
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      5136..5221
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5313..5340
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(5406..9509)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(9602..10396)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9713..9732)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10323..10342
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ctgcatgcct gcgcgcttca tgtcctcacg tagtgccaaa ggaacgcgtg cggtgaccac
       61 gacgggctta gcctttgcct gcgcttctag tgcttcgatg gtggcttgtg cctgcgcttg
      121 ctgcgcctgt agtgcctgtt gagcttcttg tagttgctgt tctagctgtg ccttggttgc
      181 catgctttaa gactctagta gctttcctgc gatatgtcat gcgcatgcgt agcaaacatt
      241 gtcctgcaac tcattcatta tgtgcagtgc tcctgttact agtcgtacat actcatattt
      301 acctagtctg catgcagtgc atgcacatgc agtcatgtcg tgctaatgtg taaaacatgt
      361 acatgcagat tgctgggggt gcagggggcg gagccaccct gtccatgcgg ggtgtggggc
      421 ttgccccgcc ggtacagaca gtgagcaccg gggcacctag tcgcggatac cccccctagg
      481 tatcggacac gtaaccctcc catgtcgatg caaatcttta acattgagta cgggtaagct
      541 ggcacgcata gccaagctag gcggccacca aacaccacta aaaattaata gtccctagac
      601 aagacaaacc cccgtgcgag ctaccaactc atatgcacgg gggccacata acccgaaggg
      661 gtttcaattg acaaccatag cactagctaa gacaacgggc acaacacccg cacaaactcg
      721 cactgcgcaa ccccgcacaa catcgggtct aggtaacact gaaatagaag tgaacacctc
      781 taaggaaccg caggtcaatg agggttctaa ggtcactcgc gctagggcgt ggcgtaggca
      841 aaacgtcatg tacaagatca ccaatagtaa ggctctggcg gggtgccata ggtggcgcag
      901 ggacgaagct gttgcggtgt cctggtcgtc taacggtgct tcgcagtttg agggtctgca
      961 aaactctcac tctcgctggg ggtcatctct ggctgaattg gaagtcatgg gcgaacgccg
     1021 cattgagctg gctattgcta ctaagaatca cttggcggcg ggtggcgcgc tcatgatgtt
     1081 tgtgggcact gttcgacaca accgctcaca gtcatttgcg caggttgaag cgggtattaa
     1141 gactgcgtac tcttcgatgg tgaaaacatc tcagtggaag aaagaacgtg cacggtacgg
     1201 ggtggagcac acctatagtg actatgaggt cacagactct tgggcgaacg gttggcactt
     1261 gcaccgcaac atgctgttgt tcttggatcg tccactgtct gacgatgaac tcaaggcgtt
     1321 tgaggattcc atgttttccc gctggtctgc tggtgtggtt aaggccggta tggacgcgcc
     1381 actgcgtgag cacggggtca aacttgatca ggtgtctacc tggggtggag acgctgcgaa
     1441 aatggcaacc tacctcgcta agggcatgtc tcaggaactg actggctccg ctactaaaac
     1501 cgcgtctaag gggtcgtaca cgccgtttca gatgttggat atgttggccg atcaaagcga
     1561 cgccggcgag gatatggacg ctgttttggt ggctcggtgg cgtgagtatg aggttggttc
     1621 taaaaacctg cgttcgtcct ggtcacgtgg ggctaagcgt gctttgggca ttgattacat
     1681 agacgctgat gtacgtcgtg aaatggaaga agaactgtac aagctcgccg gtctggaagc
     1741 accggaacgg gtcgaatcaa cccgcgttgc tgttgctttg gtgaagcccg atgattggaa
     1801 actgattcag tctgatttcg cggttaggca gtacgttctc gattgcgtgg ataaggctaa
     1861 ggacgtggcc gctgcgcaac gtgtcgctaa tgaggtgctg gcaagtctgg gtgtggattc
     1921 caccccgtgc atgatcgtta tggatgatgt ggacttggac gcggttctgc ctactcatgg
     1981 ggacgctact aagcgtgatc tgaatgcggc ggtgttcgcg ggtaatgagc agactattct
     2041 tcgcacccac taaaagcggc ataaaccccg ttcgatattt tgtgcgatga atttatggtc
     2101 aatgtcgcgg gggcaaacta tgatgggtct tgttgttggc gtcccggagc gtaaaggatc
     2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2221 cactgagcgt cagaccttga tgataccgct gccttactgg gtgcattagc cagtctgaat
     2281 gacctgtcac gggataatcc gaagtggtca gactggaaaa tcagagggca ggaactgctg
     2341 aacagcaaaa agtcagatag caccacatag cagacccgcc ataaaacgcc ctgagaagcc
     2401 cgtgacgggc ttttcttgta ttatgggtag tttccttgca tgaatccata aaaggcgcct
     2461 gtagtgccat ttacccccat tcactgccag agccgtgagc gcagcgaact gaatgtcacg
     2521 aaaaagacag cgactcaggt gcctgatggt cggagacaaa aggaatattc agcgatttgc
     2581 ccgagcttgc gagggtgcta cttaagcctt tagggtttta aggtctgttt tgtagaggag
     2641 caaacagcgt ttgcgacatc cttttgtaat actgcggaac tgactaaagt agtgagttat
     2701 acacagggct gggatctatt ctttttatct ttttttattc tttctttatt ctataaatta
     2761 taaccacttg aatataaaca aaaaaaacac acaaaggtct agcggaattt acagagggtc
     2821 tagcagaatt tacaagtttt ccagcaaagg tctagcagaa tttacagata cccacaactc
     2881 aaaggaaaag gactagtaat tatcattgac tagcccatct caattggtat agtgattaaa
     2941 atcacctaga ccaattgaga tgtatgtctg aattagttgt tttcaaagca aatgaactag
     3001 cgattagtcg ctatgactta acggagcatg aaaccaagct aattttatgc tgtgtggcac
     3061 tactcaaccc cacgattgaa aaccctacaa ggaaagaacg gacggtatcg ttcacttata
     3121 accaatacgc tcagatgatg aacatcagta gggaaaatgc ttatggtgta ttagctaaag
     3181 caaccagaga gctgatgacg agaactgtgg aaatcaggaa tcctttggtt aaaggctttg
     3241 agattttcca gtggacaaac tatgccaagt tctcaagcga aaaattagaa ttagttttta
     3301 gtgaagagat attgccttat cttttccagt taaaaaaatt cataaaatat aatctggaac
     3361 atgttaagtc ttttgaaaac aaatactcta tgaggattta tgagtggtta ttaaaagaac
     3421 taacacaaaa gaaaactcac aaggcaaata tagagattag ccttgatgaa tttaagttca
     3481 tgttaatgct tgaaaataac taccatgagt ttaaaaggct taaccaatgg gttttgaaac
     3541 caataagtaa agatttaaac acttacagca atatgaaatt ggtggttgat aagcgaggcc
     3601 gcccgactga tacgttgatt ttccaagttg aactagatag acaaatggat ctcgtaaccg
     3661 aacttgagaa caaccagata aaaatgaatg gtgacaaaat accaacaacc attacatcag
     3721 attcctacct acataacgga ctaagaaaaa cactacacga tgctttaact gcaaaaattc
     3781 agctcaccag ttttgaggca aaatttttga gtgacatgca aagtaagcat gatctcaatg
     3841 gttcgttctc atggctcacg caaaaacaac gaaccacact agagaacata ctggctaaat
     3901 acggaaggat ctgaggttct tatggctctt gtatctatca gtgaagcatc aagactaaca
     3961 aacaaaagta gaacaactgt tcacctgtta actttcaagt tggcggtgca ttacaccgcc
     4021 aggctgaatt attcctctga attatcgatt acactgtatt ccccggttaa tacagaggaa
     4081 tctgcaggat cgattgtcag tggttccttt tcatccattg atactgcacg ctggatctca
     4141 attgatacgg gcaaatattt gaacaggcga cgaatagccg ttttctttgc catttcttcc
     4201 cagtgagtta cccacggccc gttattacca gctttactca ggctgcgcac cagctcaatc
     4261 tgtttgcgcg tcataacttc aaactgagta cctccgtctt tcagtcttgc gacagcatag
     4321 acgtgggtaa ccggggcatc ttcgttttct cccgggcggt gtattaactt ttcatcaagg
     4381 ccaaattcga agctaaactc gtcaccttca cggacaacac gggctgacag gctggcgatt
     4441 tgaccagaac ggcgagccag atcaatcatg ccgcgatagc caatgattag ctgaacgttc
     4501 tttttaccgc tcttttcgtt tttattacca aaaggcagta aatatgcatg accgagggcg
     4561 ctacctggct caagtccgag ctgtgaacac tgtacgatcg cactgacaaa actcatagtg
     4621 tcacagtttc ctaacgccgg aactttacga atttctgtgg tggcgatacg gatcatacgt
     4681 tcagccgtca tatggcgtgg aagagctgct gccagttgct ctttcattga tggctggtta
     4741 ataaaactaa tcacgtcgct atttttaact gctgctggtg cacggtttcc ctgagttttt
     4801 tgcagatcgg cttttgcgat tggtggttgc ttagtcattg tatgtcctcc tggacttcgt
     4861 ggtggctacg actttcgcag ccacgaattg gttttgcttt cactgtgata agcagcctgg
     4921 aagactgagc acacagatct agcgcattac aaaatgtgcc agttactgaa tcctaagggc
     4981 aacggcgttg attttcaaac taccagctac cctgtggaca ttcgcagggt aacggccagc
     5041 taaatacatc gaaatcaatg ctgttgaaag ccgatttcaa gatatctaac aagccgctac
     5101 cccacttacc ctacgcgcct actgatctag aatttaaata aaacgaaagg ctcagtcgaa
     5161 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     5221 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     5281 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     5341 tgcgtttcta caaactctta agcttgtcta gattaagaaa taatcttcat ctaaaatata
     5401 cttcagtcgc cgcccagctg ggagaggtcg atgcgggtct cgtacaggcc cgtgatgctc
     5461 tggtggatca gggtggcgtc gagcacctcc ttcgtgctcg tgtaccgctt ccggtcgatc
     5521 gtggtgtcga agtacttgaa ggccgcgggg gcgccgaggt tggtcagcgt gaacaggtgg
     5581 atgatgttct cggcctgctc ccggatcggc ttgtcgcggt gcttgttgta cgcggacagg
     5641 accttgtcca ggttcgcgtc ggccaggatg acgcgcttgg agaactcgga gatctgctcg
     5701 atgatctcgt cgaggtagtg cttgtgctgc tccacgaaca gctgcttctg ctcgttgtcc
     5761 tccggcgagc ccttcagctt ctcgtagtgc gaggcgaggt acaggaagtt gacgtacttg
     5821 ctcggcagcg ccagctcgtt gcccttctgc agctcgcccg cgctggcgag catgcgcttc
     5881 cggccgttct ccagctcgaa cagggagtac ttggggagct tgatgatgag gtccttcttc
     5941 acctccttgt agcccttggc ctccaggaag tcgatggggt tcttctcgaa cgacgaccgc
     6001 tccatgatgg tgatgcccag cagctccttc acggacttca gcttcttgct cttgcccttc
     6061 tccaccttgg cgaccacgag gaccgagtag gcgacggtgg gggagtcgaa gccgccgtac
     6121 ttcttggggt cccagtcctt cttccgggcg atcagcttgt cgctgttccg cttgggcagg
     6181 atggactcct tgctgaagcc gccggtctgg acctccgtct tcttgacgat gttgacctgg
     6241 ggcatggaca gcaccttccg gacggtcgcg aagtcgcggc ccttgtccca cacgatctcg
     6301 cccgtctcgc cgttggtctc gatgagcggg cgcttgcgga tctcgccgtt cgccagggtg
     6361 atctcggtct tgaagaagtt catgatgttg ctgtagaaga agtacttcgc cgtggccttg
     6421 ccgatctcct gctcgctctt cgcgatcatc ttccgcacgt cgtacacctt gtagtcgccg
     6481 tagacgaact cgctctcgag cttcgggtac ttcttgatca gcgccgtgcc caccaccgcg
     6541 ttcaggtacg cgtcgtgggc gtggtggtag ttgttgatct cccgcacctt gtagaactgg
     6601 aagtccttgc ggaagtcgct caccagcttg gacttgaggg tgatgacctt gacctcgcgg
     6661 atcagcttgt cgttctcgtc gtacttggtg ttcatgcgcg agtccaggat ctgggccacg
     6721 tgcttcgtga tctgccgcgt ctcgaccagc tgccgcttga tgaagcccgc cttgtcgagc
     6781 tcgctgaggc cgccccgctc cgccttcgtc aggttgtcga acttgcgctg cgtgatgagc
     6841 ttggcgttga ggagctgccg ccagtagttc ttcatcttct tcacgacctc ctccgacggg
     6901 acgttgtcgg acttgccccg gttcttgtcc gagcgggtga ggaccttgtt gtcgatgctg
     6961 tcgtccttca ggaacgactg cgggacgatg tggtcgacgt cgtagtccga cagccggttg
     7021 atgtcgagct cctggtccac gtacatgtcc cggccgttct gcaggtagta gaggtagagc
     7081 ttctcgttct ggagctgggt gttctcgacg gggtgctcct tgaggatctg cgagccgagc
     7141 tccttgatgc cctcctcgat ccgcttcatg cgctcgcggc tgttcttctg gcccttctgc
     7201 gtggtctggt tctcccgggc catctcgatg acgatgttct cgggcttgtg gcggcccatc
     7261 accttcacca gctcgtcgac caccttgacc gtctgcagga tgcccttctt gatggccggg
     7321 ctgccggcca ggttcgcgat gtgctcgtgc agggagtcgc cctggcccga cacctgcgcc
     7381 ttctggatgt cctccttgaa cgtcaggctg tcgtcgtgga tcagctgcat gaagttgcgg
     7441 ttggcgaagc cgtcggactt caggaagtcg aggatggtct tgcccgactg cttgtcccgg
     7501 atgccgttga tgagcttgcg ggagagccgg ccccagccgg tgtaccgccg ccgcttgagc
     7561 tgcttcatga ccttgtcgtc gaacaggtgc gcgtaggtct tgaggcgctc ctcgatcatc
     7621 tcccggtcct cgaagagcgt cagggtcagc acgatgtcct ccaggatgtc ctcgttctcc
     7681 tcgttgtcga ggaagtcctt gtccttgatg atcttcagga ggtcgtggta cgtgcccagc
     7741 gacgcgttga agcggtcctc cacgccgctg atctcgaccg agtcgaagca ctcgatcttc
     7801 ttgaagtagt cctccttcag ctgcttgacg gtgaccttcc ggttcgtctt gaacagcagg
     7861 tccacgatgg ccttcttctg ctcgccggac aggaacgccg gcttccgcat gccctccgtc
     7921 acgtacttca ccttggtgag ctcgttgtag accgtgaagt actcgtacag gaggctgtgc
     7981 ttgggcagca ccttctcgtt cggcaggttc ttgtcgaagt tggtcatccg ctcgatgaac
     8041 gactgcgcgg aggcgccctt gtccaccacc tcctcgaagt tccacggggt gatcgtctcc
     8101 tccgacttgc gcgtcatcca cgcgaagcgg gagttgccgc gggcgagggg gccgacgtag
     8161 tacgggatcc ggaacgtgag gatcttctcg atcttctcgc ggttgtcctt gaggaacggg
     8221 tagaagtcct cctggcgccg gaggatggcg tgcagctcgc ccaggtggat ctggtggggg
     8281 atggagccgt tgtcgaacgt gcgctgcttg cgcaggaggt cctcccggtt cagcttgacc
     8341 agcagctcct cggtgccgtc catcttctcc aggatgggct tgatgaactt gtagaactcc
     8401 tcctgggacg cgccgccgtc gatgtagccc gcgtagccgt tcttggactg gtcgaagaag
     8461 atctccttgt acttctccgg gagctgctgg cggaccaggg ccttcagcag ggtgaggtcc
     8521 tggtggtgct cgtcgtagcg cttgatcatg ctcgcggaca ggggcgcctt ggtgatctcg
     8581 gtgttgacgc ggaggatgtc gctgagcagg atcgcgtcgg agaggttctt cgccgcgagg
     8641 aacaggtccg cgtactggtc gccgatctgc gccaggaggt tgtccaggtc gtcgtcgtac
     8701 gtgtccttgg acagctggag cttggcgtcc tcggcgaggt cgaagttgga cttgaagttc
     8761 ggcgtcaggc ccagggagag cgcgatcagg ttgccgaaga ggccgttctt cttctcgccc
     8821 ggcagctggg cgatcaggtt ctcgagccgc cgggacttcg agaggcgggc cgacaggatg
     8881 gccttcgcgt ccacgccgga ggcgttgatc gggttctcct cgaagagctg gttgtaggtc
     8941 tgcacgagct ggatgaacag cttgtcgacg tccgagttgt ccgggttcag gtcgccctcg
     9001 atgaggaagt ggccgcggaa cttgatcatg tgggcgaggg ccaggtagat caggcgcagg
     9061 tcggccttgt cggtcgagtc caccagcttc ttccggaggt ggtagatggt cgggtacttc
     9121 tcgtggtagg ccacctcgtc cacgatgttg ccgaagatgg ggtgccgctc gtgcttcttg
     9181 tcctcctcga ccaggaagga ctcctccagc cggtggaaga acgagtcgtc gaccttcgcc
     9241 atctcgttgg agaagatctc ctggaggtag cagatgcggt tcttgcggcg cgtgtaccgc
     9301 cgccgcgccg tgcgcttcag gcgggtcgcc tcggccgtct cgccgctgtc gaacaggagg
     9361 gcgccgatga ggttcttctt gatgctgtgg cggtccgtgt tgcccagcac cttgaacttc
     9421 ttcgacggga ccttgtactc gtccgtgatg acggcccagc cgacggagtt ggtgccgatg
     9481 tccaggccga tggagtactt cttgtccatc gttcaagtcc tttccaattc cacacatggt
     9541 accacacgat gattaattgt aaacagctca ggtcatgatt ccgcgaaccc cagagtcccg
     9601 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     9661 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     9721 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     9781 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     9841 cgacgagatc ctcgccgtcg ggcatccgcg ccttgagcct ggcgaacagt tcggctggcg
     9901 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     9961 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
    10021 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
    10081 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
    10141 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
    10201 gcgctgcctc gtcttggagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
    10261 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
    10321 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
    10381 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
    10441 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
    10501 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
    10561 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
    10621 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
    10681 gactggcttt ctacgtgttc cgcttccggt gaccgcctcg atgatcgccg ggtgggcgtg
    10741 gcccaaggag gatcatccag ccattcgggg tcgttcactg gttccccttt ctgatttctg
    10801 gcatagaaga acccccgtga actgtgtggt tccgggggtt gctgattttt gcgagacttc
    10861 tcgcgcaatt ccctagctta ggtgaaaaca ccatgaaaca ctagggaaac acccatgaaa
    10921 cacccattag ggcagtaggg cggcttcttc gtctagggct tgcatttggg cggtgatctg
    10981 gtctttagcg tgtgaaagtg tgtcgtaggt ggcgtgctca atgcactcga acgtcacgtc
    11041 atttaccggg tcacggtggg caaagagaac tagtgggtta gacattgttt tcctcgttgt
    11101 cggtggtggt gagcttttct agccgctcgg taaacgcggc gatcatgaac tcttggaggt
    11161 tttcaccgtt
//