| 质粒名称: | pRS426_GFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002307 |
| 产品用途: | Expression of GFP in yeast cells |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pRS426 |
| 表达系统: | 酵母表达 |
| 启动子: | TDH3 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
|
插入基因信息 |
|
| 插入基因名称: | GFP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Boosting energy metabolism and biosynthesis in diverse organisms by a common bacterial salvage lipoylation protein
Runqing Yang, Yingying Wang, Minghua Kong, Zhijuan Hu,Zhe Zhang, Kun Shen, Jiali Meng & An-Ping Zeng
Nature Communications,16,20250809
|
pRS426_GFP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pRS426_GFP载体质粒基本信息
pRS426_GFP质粒图谱载体图谱和pRS426_GFP载体序列质粒序列多克隆位点信息
pRS426_GFP质粒载体简介
pRS426_GFP质粒序列载体序列
LOCUS pRS426_GFP 7408 bp DNA circular SYN 13-MAY-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7408)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7408
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 615..636
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 651..681
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 689..705
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 713..729
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 750..768
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 805..821
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
terminator 826..1297
/label=SSD1 terminator
CDS complement(1302..2015)
/label=GFP
promoter complement(2020..2307)
/gene="S. cerevisiae TDH3"
/label=TDH3 promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase; also known as the TDH3 promoter"
primer_bind complement(2548..2564)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(2590..2608)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2618..2634)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 2775..3230
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(3361..4164)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(4165..4380)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
rep_origin 4642..5984
/label=2u ori
/note="yeast 2u plasmid origin of replication"
protein_bind 4974..5021
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
promoter 6011..6115
/gene="bla"
/label=AmpR promoter
CDS 6116..6976
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin join(7147..7408,1..327)
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
61 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
121 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
181 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
241 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
301 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
361 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
421 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
481 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
541 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
601 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
661 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
721 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
781 gagctccacc gcggtggcgg ccgctctaga actagtggat ccccccgacc tcaaatgttt
841 acgcagtagt ttctgaagta cgagcaaaat tgctcgacga tgatttcgtt attgtttccg
901 gaaaaattac ccagcgcata aaaagtaagc tttcagtact tctctttgat gatatttgta
961 cagcaaaagg gtatttagta acggaataaa tttaatatat aagagtataa ataacaaaag
1021 acaatataaa ttgaaaagtt gtaaatattg aaaagaaggc tgcattattg ttttatgtat
1081 ttttgggaaa aaatatatga cttaacgagg tcgaataaaa aaatttggat gatgccttac
1141 cggaacgtgg attaaaagga gaagtacaaa attcatgcag ttgttaatgt tcgcaggaaa
1201 taaaaacaaa gggaagggat actgaggggt gaagcacaat ccaaagaacg aaagtgaaaa
1261 acaagaaaaa cagcaatgac gatattggta gaagagagct atttttacaa ttcatccata
1321 cccaaagtaa taccagcagc ggtaacaaat tccaataaaa ccatatgatc tctcttttca
1381 tttggatctt ttgacaactt tgattgagta gacaagtaat gattatctgg caacaaaact
1441 ggaccatcac caattggggt gttttgttga taatgatcag ccaattgaac tgaaccatct
1501 tcaatgttat gtctaatctt gaagttaact ttaataccgt tcttttgttt atcagccata
1561 atgtaaacgt tatgagaatt gtagttatat tccaacttat gacccaaaat attaccatct
1621 tccttgaaat caataccctt caattcaatt ctgttaacta aagtatcacc ttcaaactta
1681 acttcagctc tggtcttgta attaccatca tccttgaaga aaatagttct ttcttgaaca
1741 taaccttctg gcatagcaga tttgaagaaa tcatgttgct tcatatgatc tggatatcta
1801 gagaaacatt gaacaccata agttaaagta gtaaccaaag ttggccatgg aacaggcaat
1861 ttaccggtgg tacaaataaa cttcaaagtc aatttaccat aagtagcatc accttcacct
1921 tcaccggaaa cagaaaattt atgaccatta acatcaccat ccaattcaac caaaattgga
1981 acaacaccag taaacaattc ttcaccttta gaaaccatct ttgtttgttt atgtgtgttt
2041 attcgaaact aagttcttgg tgttttaaaa ctaaaaaaaa gactaactat aaaagtagaa
2101 tttaagaagt ttaagaaata gatttacaga attacaatca atacctaccg tctttatata
2161 cttattagtc aagtagggga ataatttcag ggaactggtt tcaacctttt ttttcagctt
2221 tttccaaatc agagagagca gaaggtaata gaaggtgtaa gaaaatgaga tagatacatg
2281 cgtgggtcaa ttgccttgtg tcatcattta ctccaggcag gttgcatcac tccattgagg
2341 ttgtgcccgt tttttgcctg tttgtgcccc tgttctctgt agttgcgcta agagaatgga
2401 cctatgaact gatggttggt gaagaaaaca atattttggt gctgggattc ttttttttct
2461 ggatgccagc ttaaaaagcg ggctccatta tatttagtgg atgccaggaa taaactgtgg
2521 gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac
2581 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
2641 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
2701 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
2761 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
2821 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
2881 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
2941 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
3001 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
3061 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
3121 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
3181 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg
3241 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagggtaata actgatataa
3301 ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt
3361 ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc
3421 accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc
3481 agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt
3541 gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc
3601 catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac
3661 agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat
3721 caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat
3781 acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac
3841 acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa
3901 gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga
3961 aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc
4021 aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg
4081 cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg
4141 ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg
4201 cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata
4261 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc
4321 aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa
4381 aaggtggtat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
4441 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
4501 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
4561 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
4621 ataataatgg tttcttagta tgatccaata tcaaaggaaa tgatagcatt gaaggatgag
4681 actaatccaa ttgaggagtg gcagcatata gaacagctaa agggtagtgc tgaaggaagc
4741 atacgatacc ccgcatggaa tgggataata tcacaggagg tactagacta cctttcatcc
4801 tacataaata gacgcatata agtacgcatt taagcataaa cacgcactat gccgttcttc
4861 tcatgtatat atatatacag gcaacacgca gatataggtg cgacgtgaac agtgagctgt
4921 atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc tttgaagttc
4981 ctattccgaa gttcctattc tctagaaagt ataggaactt cagagcgctt ttgaaaacca
5041 aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc accggactgt aacgagctac
5101 taaaatattg cgaataccgc ttccacaaac attgctcaaa agtatctctt tgctatatat
5161 ctctgtgcta tatccctata taacctaccc atccaccttt cgctccttga acttgcatct
5221 aaactcgacc tctacatttt ttatgtttat ctctagtatt actctttaga caaaaaaatt
5281 gtagtaagaa ctattcatag agtgaatcga aaacaatacg aaaatgtaaa catttcctat
5341 acgtagtata tagagacaaa atagaagaaa ccgttcataa ttttctgacc aatgaagaat
5401 catcaacgct atcactttct gttcacaaag tatgcgcaat ccacatcggt atagaatata
5461 atcggggatg cctttatctt gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg
5521 cgggaagtgg agtcaggctt tttttatgga agagaaaata gacaccaaag tagccttctt
5581 ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag actgcattat agagcgcaca
5641 aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa tagcgctctc gggatgcatt
5701 tttgtagaac aaaaaagaag tatagattct ttgttggtaa aatagcgctc tcgcgttgca
5761 tttctgttct gtaaaaatgc agctcagatt ctttgtttga aaaattagcg ctctcgcgtt
5821 gcatttttgt tttacaaaaa tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg
5881 ttgcatttct gttctgtaaa aatgcagctc agattctttg tttgaaaaat tagcgctctc
5941 gcgttgcatt tttgttctac aaaatgaagc acagatgctt cgttcaggtg gcacttttcg
6001 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
6061 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
6121 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
6181 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
6241 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
6301 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
6361 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
6421 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
6481 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
6541 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
6601 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
6661 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
6721 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
6781 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
6841 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
6901 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
6961 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
7021 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
7081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
7141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
7201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
7261 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttgggcca
7321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
7381 ggctgctgcc agtggcgata agtcgtgt
//
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