| 质粒名称: | pIU210 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002915 |
| 产品用途: | Mammalian constitutive expression vector for Type-C TMP-inducible ssTNK secretion (PhCMV-ssTNK-FCS-ecDHFRN18T, A19V-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pcDNA3.1
|
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ssTNK, ecDHFRN18T, A19V |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pIU210
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pIU210载体质粒基本信息
pIU210质粒图谱载体图谱和pIU210载体序列质粒序列多克隆位点信息
pIU210质粒载体简介
pIU210质粒序列载体序列
LOCUS pIU210 8651 bp DNA circular SYN 22-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8651)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8651
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 147..166
/label=olyl254
primer_bind 214..250
/label=oSCL2300
primer_bind 214..235
/label=oIU199-1
misc_feature 232
/label=CMV
misc_feature 234..1300
/label=P_EF1a
primer_bind 234..258
/label=P_EF1a F
primer_bind 234..257
/label=oIU216-1
primer_bind 234..250
/label=olyl254
primer_bind 869..878
/label=oIU54-3
primer_bind 1150..1170
/label=P-EF1a-SEQ-F
primer_bind 1248..1268
/label=pEF-F
primer_bind complement(1271..1306)
/label=P_EF1a R
primer_bind complement(1272..1348)
/label=oIU97-2
primer_bind complement(1272..1306)
/label=oIU67-2
primer_bind complement(1283..1306)
/label=oIU199-2
primer_bind complement(1283..1306)
/label=oIU216-2
primer_bind 1287..1330
/label=oIU216-3
primer_bind 1287..1328
/label=oIU148-1
primer_bind 1307..1345
/label=PLAT-FP oYS606
primer_bind 1307..1328
/label=oIU181-1
primer_bind 1307..1328
/label=oIU200-1
primer_bind 1307..1328
/label=oIU216-5
primer_bind 1307..1328
/label=oIU221-1
primer_bind 1307..1323
/label=oYX527
primer_bind 1307..1323
/label=pYX561
gene 1310..1373
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/label=PLAT
/note="plasminogen activator, tissue type"
/db_xref="GeneID:5327"
/db_xref="HGNC:HGNC:9051"
/db_xref="MIM:173370"
exon 1310..1373
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
CDS 1310..1373
/codon_start=1
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/product="tissue-type plasminogen activator isoform 1
preproprotein"
/EC_number="3.4.21.68"
/label=PLAT
/note="isoform 1 preproprotein is encoded by transcript
variant 1; t-plasminogen activator; alteplase; reteplase;
plasminogen/activator kringle"
/db_xref="CCDS:CCDS6126.1"
/db_xref="GeneID:5327"
/db_xref="HGNC:HGNC:9051"
/db_xref="MIM:173370"
/protein_id="NP_000921.1"
/translation="DAMKRGLCCVLLLCGAVFVSP"
sig_peptide 1310..1372
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="COORDINATES:same species:SignalP"
primer_bind 1310..1330
/label=197-P3
primer_bind complement(1353..1426)
/label=oIU210-2
misc_feature 1373..2074
/label=Fc
misc_feature 1407..2074
primer_bind 1407..1427
/label=oIU210-3
primer_bind complement(2064..2086)
/label=oIU210-4
primer_bind 2067..2110
/label=oIU210-5
gene 2087..3706
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/label=PLAT
/note="plasminogen activator, tissue type"
/db_xref="GeneID:5327"
/db_xref="HGNC:HGNC:9051"
/db_xref="MIM:173370"
CDS 2087..3706
/codon_start=1
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/product="tissue-type plasminogen activator isoform 1
preproprotein"
/EC_number="3.4.21.68"
/label=PLAT
/note="isoform 1 preproprotein is encoded by transcript
variant 1; t-plasminogen activator; alteplase; reteplase;
plasminogen/activator kringle"
/db_xref="CCDS:CCDS6126.1"
/db_xref="GeneID:5327"
/db_xref="HGNC:HGNC:9051"
/db_xref="MIM:173370"
/protein_id="NP_000921.1"
/translation="SQEIHARFRRGARSYQVICRDEKTQMIYQQHQSWLRPVLRSNRVE
YCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRAT
CYEDQGISYRGNWSTAESGAECTNWQSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRD
SKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMIL
IGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQ
YSQPQFRIKGGLFADIASHPWQAAIFAAAAASPGERFLCGGILISSCWILSAAHCFQER
FPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQ
ESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQH
LLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQ
KDVPGVYTKVTNYLDWIRDNMRP"
exon 2087..2092
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
exon 2093..2135
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
mat_peptide 2126..3706
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/product="Tissue-type plasminogen activator.
/id=PRO_0000028350"
/note="propagated from UniProtKB/Swiss-Prot (P00750.1)"
mat_peptide 2126..2950
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/product="Tissue-type plasminogen activator chain A.
/id=PRO_0000028351"
/note="propagated from UniProtKB/Swiss-Prot (P00750.1)"
primer_bind 2126..2147
/label=222-P2
exon 2136..2273
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
misc_feature 2144..2176
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/note="propagated from UniProtKB/Swiss-Prot (P00750.1);
Region: Important for binding to annexin A2"
exon 2274..2384
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
misc_feature 2306..2308
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/note="O-linked (Fuc) threonine.
/evidence=ECO:0000269|PubMed:1900431. /id=CAR_000029;
propagated from UniProtKB/Swiss-Prot (P00750.1);
glycosylation site"
misc_feature 2324..2326
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/note="Important for binding to LRP1; propagated from
UniProtKB/Swiss-Prot (P00750.1); other site"
exon 2385..2559
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
primer_bind complement(2415..2466)
/label=198-P7
primer_bind complement(2415..2432)
/label=198-P5
primer_bind complement(2430..2464)
/label=198-P1
misc_feature 2432..2434
/label=T103N
primer_bind 2445..2479
/label=198-P2
primer_bind 2449..2496
/label=198-P8
misc_feature 2474..2476
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000269|PubMed:2513186; propagated from
UniProtKB/Swiss-Prot (P00750.1); glycosylation site"
misc_feature 2474..2476
/label=N117Q
primer_bind 2477..2496
/label=198-P6
exon 2560..2651
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
exon 2652..2823
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
misc_feature 2675..2677
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/note="N-linked (GlcNAc...) asparagine, partial.
/evidence=ECO:0000269|PubMed:2513186. /id=CAR_000030;
propagated from UniProtKB/Swiss-Prot (P00750.1);
glycosylation site"
primer_bind 2715..2732
/label=oIU148-5
misc_feature 2777..2779
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/note="Not glycosylated; propagated from
UniProtKB/Swiss-Prot (P00750.1); other site"
exon 2824..2909
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
primer_bind complement(2886..2905)
/label=TNK-Mid
primer_bind 2888..2908
/label=oIU210-T
exon 2910..3105
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
mat_peptide 2951..3706
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/product="Tissue-type plasminogen activator chain B.
/id=PRO_0000028352"
/note="propagated from UniProtKB/Swiss-Prot (P00750.1)"
primer_bind complement(2993..3022)
/label=198-P3
primer_bind 3005..3040
/label=198-P4
variation 3011..3022
/label=KHRR 296-299 AAAA
exon 3106..3242
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
exon 3243..3383
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
exon 3384..3550
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
misc_feature 3410..3412
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/note="Important for single-chain activity; propagated
from UniProtKB/Swiss-Prot (P00750.1); other site"
misc_feature 3467..3469
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000269|PubMed:2513186. /id=CAR_000031;
propagated from UniProtKB/Swiss-Prot (P00750.1);
glycosylation site"
exon 3551..3706
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/inference="alignment:same species:2.1.0"
misc_feature 3554..3556
/gene="PLAT"
/gene_synonym="T-PA; TPA"
/note="Important for single-chain activity; propagated
from UniProtKB/Swiss-Prot (P00750.1); other site"
primer_bind complement(3685..3727)
/label=oIU148-2
primer_bind complement(3685..3706)
/label=oIU181-2
primer_bind complement(3685..3706)
/label=oIU209-2
primer_bind complement(3686..3706)
/label=oYX528
primer_bind complement(3688..3706)
/label=197-P4
primer_bind complement(3706..3747)
/label=oIU210-6
CDS 3706
/label=EGFP
misc_feature 3707..3718
/label=FCS
primer_bind 3719..3751
/label=OGT150-2-F
CDS 3734..4212
/codon_start=1
/label=ecDHFR(N18T/A19V)
/translation="MISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRH
TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFL
PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
primer_bind complement(4188..4233)
/label=oIU216-6
primer_bind complement(4188..4213)
/label=oIU221-2
primer_bind complement(4188..4210)
/label=OGT150-2-R
primer_bind complement(4221..4256)
/label=oIU216-4
terminator 4248..4475
/label=bGH PA
misc_feature 5143..5220
/label=SV40 ORI
CDS 5362..6153
/label=Kan/neoR
primer_bind complement(6493..6513)
/label=M13-rev
misc_binding complement(6519..6541)
/label=LacO
promoter complement(6546..6575)
/label=lac
CDS complement(7658..8317)
/label=AmpR
promoter complement(8557..8585)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgggaaag
241 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
301 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
361 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
421 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga aagcttatgg atgcaatgaa
1321 gagagggctc tgctgtgtgc tgctgctgtg tggagcagtc ttcgtttcgc ccatatcggc
1381 catggttaga tctgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg
1441 gggaccgtca gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac
1501 ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa
1561 ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta
1621 caacagcacg taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg
1681 caaggagtac aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat
1741 ctccaaagcc aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga
1801 ggagatgacc aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga
1861 catcgccgtg gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc
1921 cgtgctggac tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag
1981 gtggcagcag gggaacgtct tctcatgctc cgtgatgcac gaggctctgc acaaccacta
2041 cacgcagaag agcctctccc tgtctccggg taaagccagc ggaagtagcc aggaaatcca
2101 tgcccgattc agaagaggag ccagatctta ccaagtgatc tgcagagatg aaaaaacgca
2161 gatgatatac cagcaacatc agtcatggct gcgccctgtg ctcagaagca accgggtgga
2221 atattgctgg tgcaacagtg gcagggcaca gtgccactca gtgcctgtca aaagttgcag
2281 cgagccaagg tgtttcaacg ggggcacctg ccagcaggcc ctgtacttct cagatttcgt
2341 gtgccagtgc cccgaaggat ttgctgggaa gtgctgtgaa atagatacca gggccacgtg
2401 ctacgaggac cagggcatca gctacagggg caattggagc acagcggaga gtggcgccga
2461 gtgcaccaac tggcaaagca gcgcgttggc ccagaagccc tacagcgggc ggaggccaga
2521 cgccatcagg ctgggcctgg ggaaccacaa ctactgcaga aacccagatc gagactcaaa
2581 gccctggtgc tacgtcttta aggcggggaa gtacagctca gagttctgca gcacccctgc
2641 ctgctctgag ggaaacagtg actgctactt tgggaatggg tcagcctacc gtggcacgca
2701 cagcctcacc gagtcgggtg cctcctgcct cccgtggaat tccatgatcc tgataggcaa
2761 ggtttacaca gcacagaacc ccagtgccca ggcactgggc ctgggcaaac ataattactg
2821 ccggaatcct gatggggatg ccaagccctg gtgccacgtg ctgaagaacc gcaggctgac
2881 gtgggagtac tgtgatgtgc cctcctgctc cacctgcggc ctgagacagt acagccagcc
2941 tcagtttcgc atcaaaggag ggctcttcgc cgacatcgcc tcccacccct ggcaggctgc
3001 catctttgcc gctgcggccg cctcgcccgg agagcggttc ctgtgcgggg gcatactcat
3061 cagctcctgc tggattctct ctgccgccca ctgcttccag gagaggtttc cgccccacca
3121 cctgacggtg atcttgggca gaacataccg ggtggtccct ggcgaggagg agcagaaatt
3181 tgaagtcgaa aaatacattg tccataagga attcgatgat gacacttacg acaatgacat
3241 tgcgctgctg cagctgaaat cggattcgtc ccgctgtgcc caggagagca gcgtggtccg
3301 cactgtgtgc cttcccccgg cggacctgca gctgccggac tggacggagt gtgagctctc
3361 cggctacggc aagcatgagg ccttgtctcc tttctattcg gagcggctga aggaggctca
3421 tgtcagactg tacccatcca gccgctgcac atcacaacat ttacttaaca gaacagtcac
3481 cgacaacatg ctgtgtgctg gagacactcg gagcggcggg ccccaggcaa acttgcacga
3541 cgcctgccag ggcgattcgg gaggccccct ggtgtgtctg aacgatggcc gcatgacttt
3601 ggtgggcatc atcagctggg gcctgggctg tggacagaag gatgtcccgg gtgtgtacac
3661 caaggttacc aactacctag actggattcg tgacaacatg cgaccgagga tcaagagggg
3721 ttccggagaa ttcatgatca gcctgatcgc cgccctggcc gtggaccacg tgatcggcat
3781 ggagaccgtg atgccctgga acctgcccgc cgacctggcc tggttcaaga gaaacaccct
3841 gaacaagccc gtgatcatgg gcagacacac ctgggagagc atcggcagac ccctgcccgg
3901 cagaaagaac atcatcctga gcagccagcc cagcaccgac gacagagtga cctgggtgaa
3961 gagcgtggac gaggccatcg ccgcctgcgg cgacgtgccc gagatcatgg tgatcggcgg
4021 cggcagagtg tacgagcagt tcctgcccaa ggcccagaag ctgtacctga cccacatcga
4081 cgccgaggtg gagggcgaca cccacttccc cgactacgag cccgacgact gggagagcgt
4141 gttcagcgag ttccacgacg ccgacgccca gaacagccac agctactgct tcgagatcct
4201 ggagagaaga tagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
4261 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
4321 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
4381 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
4441 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
4501 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
4561 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
4621 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
4681 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
4741 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
4801 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
4861 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
4921 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
4981 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
5041 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
5101 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
5161 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
5221 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
5281 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
5341 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
5401 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
5461 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
5521 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
5581 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
5641 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
5701 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
5761 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
5821 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
5881 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
5941 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
6001 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
6061 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
6121 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
6181 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
6241 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
6301 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
6361 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
6421 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
6481 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
6541 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
6601 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
6661 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
6721 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
6781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
6841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
6901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
6961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
7021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
7081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
7141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
7201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
7261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
7321 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
7381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt
7441 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
7501 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
7561 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
7621 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
7681 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
7741 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
7801 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
7861 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
7921 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
7981 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
8041 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
8101 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
8161 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
8221 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
8281 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
8341 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
8401 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
8461 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
8521 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
8581 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
8641 cacctgacgt c
//
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