plx304-CAG-attP-EBFP2-P2A-Bxb1-T2A-BSD

LOCUS       plx304-CAG-attP-EBFP2-P2A-Bxb1-T2A-BSD            10716 bp DNA     circular SYN 03-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    40..402
                     /label=CMV enhancer
     misc_feature    40..44
     misc_feature    378..383
     promoter        403..679
     misc_feature    590..596
     misc_feature    653..660
     exon            680..772
     misc_feature    680
     intron          773..1651
     intron          1652..1696
     primer_bind     1690..1708
     exon            1697..1750
     misc_feature    1751..1799
                     /label=INSERT
                     /label=INSERT(1)
     misc_feature    1752..1799
                     /label=Bxb1_attP
     misc_feature    1806..1808
                     /standard_name="emiRFP670"
                     /label=START
                     /note="Geneious type: ORF"
     CDS             1809..2522
                     /codon_start=1
                     /product="blue variant of GFP"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIKV
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLLE
                     FRTAAGITLGMDELYK"
     misc_feature    2523..2588
                     /label=P2A
                     /note="Geneious type: motif"
     primer_bind     2523..2541
     misc_feature    2575..2588
                     /label=Gibson
     primer_bind     2579..2588
     CDS             2601..2621
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2622..2648
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2655..4151
                     /codon_start=1
                     /label=Bxb1
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     misc_feature    4155..4217
                     /label=2A
     misc_feature    4155..4217
                     /label=2A_peptide
     CDS             4218..4610
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4614..4619
                     /label=Nhe1
     misc_feature    4615..4629
                     /label=Gibson
     misc_feature    4645
                     /label=p_overlap
                     /label=p_overlap(1)
     misc_feature    4646..5234
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    complement(4767..4786)
                     /label=WPRE-R
     CDS             complement(5117..5128)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5306..5539
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5311..5335
                     /label=Sequencing primer_fwd_3059
     misc_feature    5359..5539
                     /label=HIV1 3' LTR
     polyA_signal    5617..5738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5778..5913
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5933..5953)
                     /label=T7
     promoter        complement(5934..5952)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5962..5979)
                     /label=M13-fwd
     primer_bind     complement(5962..5978)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6050..6118
                     /label=LacZ alpha
     rep_origin      6120..6575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      6137..6443
                     /label=F1 ori
     promoter        6601..6705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6706..7566
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             6904..7563
                     /label=AmpR
     rep_origin      7661..8343
                     /label=ColE1 origin
     rep_origin      7737..8325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8613..8634
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8649..8679
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_binding    8683..8705
                     /label=LacO
     protein_bind    8687..8703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8711..8731
                     /label=M13-rev
     primer_bind     8711..8727
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8748..8766
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     8749..8768
                     /label=T3
     promoter        8792..9020
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    8972..8997
                     /label=Fwd sequencing primer_6720
     misc_feature    9021..9201
                     /label=HIV1 5'LTR
     LTR             9021..9201
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9248..9373
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9312..9356
                     /label=Psi Packaging Signal
     misc_feature    9866..10099
                     /label=RRE
     misc_feature    10123..10145
                     /label=Sequencing primer_Rev_7893
     misc_feature    10547..10664
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttacctttcg tcttcaagaa ttcctcgagc tagttattaa tagtaatcaa
       61 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      121 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      181 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      241 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      301 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      361 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      421 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      481 ttttttaatt attttgtgca gcgatggggg cggggggggg gggcgcgcgc caggcggggc
      541 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
      601 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
      661 gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc
      721 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
      781 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
      841 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
      901 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
      961 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     1021 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     1081 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     1141 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     1201 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     1261 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg
     1321 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     1381 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     1441 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     1501 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     1561 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     1621 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     1681 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     1741 caaagaattg gggtttgtct ggtcaaccac cgcggtctca gtggtgtacg gtacaaaccg
     1801 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1861 tggacggcga cgtaaacggc cacaagttca gcgtgagggg cgagggcgag ggcgatgcca
     1921 ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1981 ccaccctcgt gaccaccctg agccacggcg tgcagtgctt cgcccgctac cccgaccaca
     2041 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2101 tcttcttcaa ggacgacggc acctacaaga cccgcgccga ggtgaagttc gagggcgaca
     2161 ccctggtgaa ccgcatcgag ctgaagggcg tcgacttcaa ggaggacggc aacatcctgg
     2221 ggcacaagct ggagtacaac ttcaacagcc acaacatcta tatcatggcc gtcaagcaga
     2281 agaacggcat caaggtgaac ttcaagatcc gccacaacgt ggaggacggc agcgtgcagc
     2341 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2401 gccactacct gagcacccag tccgtgctga gcaaagaccc caacgagaag cgcgatcaca
     2461 tggtcctgct ggagttccgc accgccgccg ggatcactct cggcatggac gagctgtaca
     2521 agggctctgg agccaccaac ttcagcctgc tgaagcaggc aggcgacgtg gaagagaacc
     2581 ctggcccttc cgccaccatg ccaaaaaaga aaagaaaagt gtatccctat gatgtccccg
     2641 attatgccgg ttcaagagcc ctggtcgtga ttagactgag ccgagtgaca gacgccacca
     2701 caagtcccga gagacagctg gaatcatgcc agcagctctg tgctcagcgg ggttgggatg
     2761 tggtcggcgt ggcagaggat ctggacgtga gcggggccgt cgatccattc gacagaaaga
     2821 ggaggcccaa cctggcaaga tggctcgctt tcgaggaaca gccctttgat gtgatcgtcg
     2881 cctacagagt ggaccggctg acccgctcaa ttcgacatct ccagcagctg gtgcattggg
     2941 ctgaggacca caagaaactg gtggtcagcg caacagaagc ccacttcgat actaccacac
     3001 cttttgccgc tgtggtcatc gcactgatgg gcactgtggc ccagatggag ctcgaagcta
     3061 tcaaggagcg aaacaggagc gcagcccatt tcaatattag ggccggtaaa tacagaggct
     3121 ccctgccccc ttggggatat ctccctacca gggtggatgg ggagtggaga ctggtgccag
     3181 accccgtcca gagagagcgg attctggaag tgtaccacag agtggtcgat aaccacgaac
     3241 cactccatct ggtggcacac gacctgaata gacgcggcgt gctctctcca aaggattatt
     3301 ttgctcagct gcagggaaga gagccacagg gaagagaatg gagtgctact gcactgaaga
     3361 gatctatgat cagtgaggct atgctgggtt acgcaacact caatggcaaa actgtccggg
     3421 acgatgacgg agcccctctg gtgagggctg agcctattct caccagagag cagctcgaag
     3481 ctctgcgggc agaactggtc aagactagtc gcgccaaacc tgccgtgagc accccaagcc
     3541 tgctcctgag ggtgctgttc tgcgccgtct gtggagagcc agcatacaag tttgccggcg
     3601 gagggcgcaa acatccccgc tatcgatgca ggagcatggg gttccctaag cactgtggaa
     3661 acgggacagt ggccatggct gagtgggacg ccttttgcga ggaacaggtg ctggatctcc
     3721 tgggtgacgc tgagcggctg gaaaaagtgt gggtggcagg atctgactcc gctgtggagc
     3781 tggcagaagt caatgccgag ctcgtggatc tgacttccct catcggatct cctgcatata
     3841 gagctgggtc cccacagaga gaagctctgg acgcacgaat tgctgcactc gctgctagac
     3901 aggaggaact ggagggcctg gaggccaggc cctctggatg ggagtggcga gaaaccggac
     3961 agaggtttgg ggattggtgg agggagcagg acaccgcagc caagaacaca tggctgagat
     4021 ccatgaatgt ccggctcaca ttcgacgtgc gcggtggcct gactcgaacc atcgattttg
     4081 gcgacctgca ggagtatgaa cagcacctga gactggggtc cgtggtcgaa agactgcaca
     4141 ctgggatgtc ctctggcagc ggagagggca gaggaagtct tctaacatgc ggtgacgtgg
     4201 aggagaatcc cggccctgcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     4261 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     4321 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     4381 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     4441 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     4501 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     4561 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagctagcg
     4621 ctaaccggtg gcgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     4681 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4741 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4801 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4861 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4921 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4981 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5041 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5101 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5161 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5221 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     5281 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5341 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5521 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     5581 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     5641 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5701 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     5761 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5821 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5881 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     5941 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     6001 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     6061 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     6121 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6181 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6241 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6301 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6361 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6421 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6481 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6541 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     6601 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6661 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6721 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6781 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6841 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6901 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6961 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7021 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7081 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7141 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7201 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7261 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7321 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7381 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7441 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7501 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7561 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7621 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7681 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7741 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7801 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7861 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7921 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7981 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8041 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8101 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8161 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8221 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8281 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8341 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8401 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8461 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8521 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8581 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8641 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8701 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     8761 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     8821 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     8881 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     8941 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     9001 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     9061 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9121 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9181 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     9241 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     9301 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     9361 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     9421 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     9481 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     9541 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     9601 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     9661 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     9721 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     9781 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9841 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9901 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9961 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    10021 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    10081 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    10141 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    10201 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    10261 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    10321 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    10381 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    10441 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    10501 cctaagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    10561 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    10621 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    10681 acagcagaga tccactttgg cgccggctcg aggggg
//