| 质粒名称: | pAAVLINK-EF1alpha-HA-5'-CTTNBP2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001853 |
| 产品用途: | 5' AAVLINK plasmid for CTTNBP2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CTTNBP2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-CTTNBP2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-CTTNBP2载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-CTTNBP2质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-CTTNBP2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-CTTNBP2质粒载体简介
pAAVLINK-EF1alpha-HA-5'-CTTNBP2质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-CTTNBP2 6873 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6873)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 10, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6873
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4013
/codon_start=1
/gene="CTTNBP2"
/gene_synonym="C7orf8; CORTBP2; Orf4"
/product="cortactin-binding protein 2 isoform 1"
/label=CTTNBP2
/note="isoform 1 is encoded by transcript variant
1;CORTBP2, C7orf8"
/db_xref="GI:16975496"
/db_xref="CCDS:CCDS5774.1"
/db_xref="GeneID:83992"
/db_xref="HGNC:HGNC:15679"
/db_xref="MIM:609772"
/protein_id="NP_219499.1"
/translation="ATDGASCEPDLSRAPEDAAGAAAEAAKKEFDVDTLSKSELRMLLS
VMEGELEARDLVIEALRARRKEVFIQERYGRFNLNDPFLALQRDYEAGAGDKEKKPVCT
NPLSILEAVMAHCKKMQERMSAQLAAAESRQKKLEMEKLQLQALEQEHKKLAARLEEER
GKNKQVVLMLVKECKQLSGKVIEEAQKLEDVMAKLEEEKKKTNELEEELSAEKRRSTEM
EAQMEKQLSEFDTEREQLRAKLNREEAHTTDLKEEIDKMRKMIEQLKRGSDSKPSLSLP
RKTKDRRLVSISVGTEGTVTRSVACQTDLVTENADHMKKLPLTMPVKPSTGSPLVSANA
KGSVCTSATMARPGIDRQASYGDLIGASVPAFPPPSANKIEENGPSTGSTPDPTSSTPP
LPSNAAPPTAQTPGIAPQNSQAPPMHSLHSPCANTSLHPGLNPRIQAARFRFQGNANDP
DQNGNTTQSPPSRDVSPTSRDNLVAKQLARNTVTQALSRFTSPQAGAPSRPGVPPTGDV
GTHPPVGRTSLKTHGVARVDRGNPPPIPPKKPGLSQTPSPPHPQLKVIIDSSRASNTGA
KVDNKTVASTPSSLPQGNRVINEENLPKSSSPQLPPKPSIDLTVAPAGCAVSALATSQV
GAWPAATPGLNQPACSDSSLVIPTTIAFCSSINPVSASSCRPGASDSLLVTASGWSPSL
TPLLMSGGPAPLAGRPTLLQQAAAQGNVTLLSMLLNEEGLDINYSCEDGHSALYSAAKN
GHTDCVRLLLSAEAQVNAADKNGFTPLCAAAAQGHFECVELLISYDANINHAADGGQTP
LYLACKNGNKECIKLLLEAGTNRSVKTTDGWTPVHAAVDTGNVDSLKLLMYHRIPAHGN
SFNEEESESSVFDLDGGEESPEGISKPVVPADLINHANREGWTAAHIAASKGFKNCLEI
LCRHGGLEPERRDKCNRTVHDVATDDCKHLLENLNALKIPLRISVGEIEPSNYGSDDLE
CENTICALNIRKQTSWDDFSKAVSQALTNHFQAISSDGWWSLEDVTCNNTTDSNIGLSA
RSIRSITLGNVPWSVGQSFAQSPWDFMRKNKAEHITVLLS"
misc_feature 945..950
/label=SDS
misc_feature 1305..1310
/label=SDS
regulatory 1790..1799
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 2369..2374
/label=SDS
misc_feature 2372..2377
/label=SDS
misc_feature 2794..2799
/label=SDS
misc_feature 3372..3377
/label=SDS
intron 4014..4054
/label=Splicing Donor
misc_feature 4058..4091
/label=lox2272-JT1571
repeat_region 4136..4276
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4136..4265
/label=AAV2 ITR
repeat_region 4137..4277
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4351..4806
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5088..5192
/gene="bla"
/label=AmpR promoter
CDS 5193..6053
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6224..6812
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctgc gacggacggc gcgagctgcg agcccgactt
781 gtcccgggcc ccggaggacg cggcgggggc cgcggcggag gcggcgaaaa aagagtttga
841 tgtggatact ctcagtaaat ccgagctgcg gatgctcctc agcgtgatgg aaggggagct
901 ggaggccaga gaccttgtca tcgaggccct gcgggctcgc aggaaggagg tatttatcca
961 ggaacggtat gggagattta atctaaatga cccgttcctg gcactccaga gagactatga
1021 agcaggtgct ggtgacaaag agaagaagcc agtttgtacc aaccccctct ccatccttga
1081 agcagtcatg gcccactgca agaaaatgca agaaaggatg tccgcacagc tggctgctgc
1141 tgagagcaga caaaagaagc tggaaatgga gaagcttcag ctacaagccc ttgagcaaga
1201 gcacaagaag ctggctgccc gccttgagga agagcgtggc aagaacaagc aggtggtcct
1261 gatgctggtc aaagagtgca agcagctctc aggcaaagtc atagaggagg cccagaagct
1321 cgaagacgta atggccaaac tggaagagga aaagaaaaag acgaatgaat tagaagagga
1381 actctccgct gagaaacgaa gaagcacaga aatggaagct cagatggaaa aacaactctc
1441 tgagtttgac actgagcggg aacagcttcg tgccaagctg aaccgggaag aagcacacac
1501 cactgacctc aaagaggaga tagacaagat gaggaaaatg attgagcaac tgaaaagggg
1561 aagtgacagc aaaccaagcc tctctcttcc acggaagaca aaagataggc gtttggtttc
1621 catatctgtg ggaacagaag gaactgtgac aaggtctgtt gcatgccaga cagacctagt
1681 gacagaaaat gctgaccaca tgaaaaagtt gcctttaacc atgcctgtaa aaccttccac
1741 agggagtccc ctagtttctg caaatgcaaa agggagcgtg tgcaccagtg ccaccatggc
1801 cagaccaggt attgacaggc aggcttccta tggtgacttg attggcgctt ctgtacccgc
1861 tttcccacct ccaagtgcaa acaaaattga ggaaaatgga ccaagcactg gctcaacacc
1921 agatccaacc agtagcacac ccccacttcc cagtaacgct gcccctccca ccgctcaaac
1981 accaggcata gctcctcaga actcgcaagc tccacctatg cacagtttac attcaccatg
2041 tgccaacacc tctttgcatc caggtctaaa cccacgaatc caagcagcta gatttagatt
2101 tcagggcaat gctaacgacc cagaccaaaa tggaaatact acccaaagtc ctccgtcaag
2161 agatgtctcg cctacaagtc gtgacaacct agtggccaaa caactagctc ggaatactgt
2221 gacccaagca ctgtcaagat ttacaagccc tcaagcaggt gctccctcaa ggcctggagt
2281 gcccccaaca ggggatgttg gcacccaccc tccagttggt cggaccagtt taaagactca
2341 tggtgtagca cgagttgaca gaggaaatcc tcctcctatc cctccaaaaa agccagggct
2401 ctcccaaact ccttctccac cacaccccca actcaaggtt attatagaca gcagcagggc
2461 ctcgaacaca ggggccaaag ttgataacaa aactgtggct tcgactcctt ccagtttgcc
2521 acaagggaac agggtgatca atgaggagaa ccttcctaag tcatcctccc ctcagctgcc
2581 accaaaacca tccatagatt taactgtggc acctgcaggc tgtgccgttt cagccctggc
2641 cacgtctcag gtgggtgcct ggcctgctgc aacccccgga ctgaaccaac ctgcatgttc
2701 agacagttcc cttgtcattc ctaccaccat tgccttttgc tcttccataa accccgttag
2761 tgcctcatcc tgtagaccag gtgcctcaga cagcctcctg gtaacagcat caggctggtc
2821 accctcccta acccctttgc taatgagtgg tggtcctgcc cccctggctg gcaggcccac
2881 ccttcttcag caagctgctg cccagggaaa tgtcacttta ttatcaatgc tgcttaatga
2941 agaaggactg gacattaatt actcctgtga agatggccat tctgccttgt attctgctgc
3001 taagaatgga catacagact gtgtgagatt gctgctgagt gcagaagccc aagtcaatgc
3061 tgctgataaa aatggcttca cacccttgtg tgctgcagct gctcagggac atttcgagtg
3121 tgtagaatta ttaatttcat atgatgctaa cattaatcat gctgctgatg gaggacagac
3181 acctctatac ctggcctgta aaaatggaaa taaagaatgt attaaactct tgttggaagc
3241 tggaaccaat cgaagtgtaa aaaccacaga tggctggaca ccagttcacg cagctgtgga
3301 cactggtaat gtggacagcc tcaagcttct tatgtaccat agaataccag ctcatggaaa
3361 ttctttcaat gaggaggagt ccgagtcaag tgtctttgac ttggatggag gagaagagag
3421 tcctgaaggc atatccaagc ctgttgttcc tgcagacctc attaaccacg ccaacagaga
3481 aggctggact gctgcccaca ttgctgcttc caaaggtttt aagaactgcc tagaaatctt
3541 gtgtaggcac ggagggcttg agccagaaag gagagacaag tgcaatcgga ctgtgcatga
3601 tgttgccact gatgactgca agcatttgct ggagaatctg aatgctctta aaataccctt
3661 aaggatttca gtgggtgaga ttgaaccaag caactatggt tctgatgact tggaatgtga
3721 aaacacaata tgtgctttaa atatccgcaa acagacatca tgggatgatt tttcaaaagc
3781 agtgagtcaa gctctgacaa atcatttcca ggcaatctct tctgatggat ggtggagtct
3841 ggaagacgtg acttgcaata acaccactga ttccaacatc ggcctcagtg caagaagcat
3901 acgatccatc acgctaggaa atgtgccgtg gtcagtgggt cagagcttcg cgcagtcccc
3961 gtgggacttt atgaggaaga ataaggcaga gcacatcact gtgcttttgt caggtaagta
4021 tcaaggttac aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta
4081 tacgaagtta ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
4141 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
4201 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
4261 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
4321 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
4381 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
4441 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
4501 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
4561 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
4621 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
4681 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
4741 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
4801 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
4861 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
4921 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
4981 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
5041 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
5101 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
5161 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
5221 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
5281 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
5341 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
5401 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
5461 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
5521 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
5581 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
5641 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
5701 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
5761 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
5821 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
5881 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
5941 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
6001 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
6061 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
6121 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
6181 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
6241 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
6301 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
6361 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
6421 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
6481 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
6541 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
6601 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
6661 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
6721 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
6781 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
6841 tcctggcctt ttgctggcct tttgctcaca tgt
//
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