pCytERM-mScarlet3

LOCUS       pCytERM-mScarlet3          4782 bp DNA     circular SYN 18-OCT-2024
DEFINITION  TSN20241013-0571-00010_745_20241014-BAN4_D02_D02_i20.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..246
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             298..384
                     /codon_start=1
                     /label=cytochrome p450
                     /translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
     regulatory      430..439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             436..1122
                     /codon_start=1
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     polyA_signal    1248..1369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1376..1831)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1858..1962
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1964..2321
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2356..3150
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3382..3429
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3758..4346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        join(4521..4782,1..42)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
       61 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      121 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      181 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      241 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctccaccatg
      301 gatcctgtgg tggtgctggg gctctgtctc tcctgtttgc ttctcctttc actctggaaa
      361 cagagctatg ggggagggaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
      421 ccaccggtcg ccaccatgga tagcaccgag gcagtgatca aggagttcat gcggttcaag
      481 gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
      541 cgcccctacg agggcaccca gaccgccaag ctgagggtga ccaagggtgg ccccctgccc
      601 ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt cacgaagcac
      661 cccgccgaca tccccgacta ctggaagcag tccttccccg agggcttcaa gtgggagcgc
      721 gtgatgaact tcgaggacgg cggcgccgtg tccgtggccc aggacacctc cctggaggac
      781 ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta
      841 atgcagaaga agacaatggg ctgggaagca tccaccgagc ggttgtaccc cgaggacgtc
      901 gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg gtacctggcg
      961 gacttcaaga ccacctacag ggccaagaag cccgtgcaga tgcccggcgc cttcaacatc
     1021 gaccgcaagt tggacatcac atcccacaac gaggactaca ccgtggtgga acagtacgaa
     1081 cgctccgtgg cccgccactc caccggcggc tccggtggct cctaaagcgg ccgcgactct
     1141 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     1201 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     1261 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1321 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     1381 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     1441 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     1501 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     1561 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     1621 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     1681 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     1741 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     1801 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     1861 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1921 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     1981 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2041 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2101 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     2161 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2221 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     2281 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     2341 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     2401 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     2461 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     2521 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     2581 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     2641 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     2701 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     2761 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     2821 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     2881 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     2941 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     3001 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     3061 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     3121 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     3181 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     3241 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     3301 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     3361 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     3421 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     3481 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     3541 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     3601 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3661 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3721 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3781 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3841 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3901 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3961 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4021 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4081 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4141 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4201 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4261 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4321 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4381 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4441 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
     4501 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     4561 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4621 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4681 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     4741 gcctggcatt atgcccagta catgacctta tgggactttc ct
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