| 质粒名称: | pAAV-CAG-eRFP1-P2A-EGFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000710 |
| 产品用途: | AAV, experess eRFP1 and EGFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | eRFP1, EGFP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
买家导航
pAAV-CAG-eRFP1-P2A-EGFP载体质粒基本信息
pAAV-CAG-eRFP1-P2A-EGFP质粒图谱载体图谱和pAAV-CAG-eRFP1-P2A-EGFP载体序列质粒序列多克隆位点信息
pAAV-CAG-eRFP1-P2A-EGFP质粒载体简介
pAAV-CAG-eRFP1-P2A-EGFP质粒序列载体序列
LOCUS pAAV-CAG-eRFP1-P2A-EGFP 6223 bp DNA circular SYN 02-FEB-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6223)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6223
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 919..941
/label=Seq-F
primer_bind 964..1004
/label=eRFP1-F
regulatory 977..982
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 983..1672
/codon_start=1
/label=eRFP1
/translation="MENVRRKTGIQTEMKTKLHMDGMVNGHSFEIKGEGKGSPYEGVQT
MKLKVTKGAPLPFSIDILLPQCMYGSKPFIKYPENIPDYLKLSFPEGITWERTMTFEDG
AVCDVSNDSRLVGNCFIYTVKFQGVNFPLDGPVMQKKTRGWEPSTEVLYECDGWMRGLV
DLAIKLENGGHYMCNFKTTYKSKKGLEVPPYHFVDHKLDLLSHNTDGATFEEFEQGEIA
HAHLSKLA"
primer_bind complement(1653..1689)
/label=eRFP1-R
CDS 1697..1753
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1766..2482
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(2459..2482)
/label=0731-XbaI-mScarlet-Rv
misc_feature 2507..3095
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2978..2989)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3138..3259
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3787..4375)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4546..5406)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5407..5511)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5537..5992)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggagaa cgtcaggaga aagacaggca ttcagacgga
1021 aatgaaaacg aagctccaca tggatggtat ggtgaatgga cacagctttg agatcaaagg
1081 ggaggggaag ggaagcccgt acgagggggt gcagacaatg aagctgaagg ttactaaggg
1141 cgccccactt cccttctcta tagacattct tttgcctcag tgcatgtatg gcagcaaacc
1201 tttcattaaa tatcctgaga acatccccga ttatttgaaa ctctcatttc cagagggtat
1261 cacttgggag aggacaatga cattcgagga cggcgcagta tgcgatgtat ctaatgattc
1321 cagactggtc ggcaattgct ttatctacac tgtgaagttt cagggagtca attttccact
1381 tgatggcccc gttatgcaaa agaagacccg aggctgggaa ccctccactg aagtgctgta
1441 cgaatgtgat gggtggatgc gcgggttggt ggacctggct atcaaactgg aaaacggtgg
1501 acattatatg tgtaatttta agaccaccta caaatccaag aaaggtcttg aggtgccacc
1561 ttaccatttc gtggatcata aactcgacct gctcagtcat aacaccgacg gagccacctt
1621 tgaggagttc gaacaaggtg aaatcgctca cgcgcacttg agtaaactgg cccgaccggt
1681 tcacgtggga agtggggcta caaacttctc actgctgaaa caggccgggg atgtggagga
1741 aaacccagga ccacacgtga ctagtgtgag caagggcgag gagctgttca ccggggtggt
1801 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
1861 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
1921 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
1981 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
2041 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
2101 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
2161 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
2221 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
2281 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
2341 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
2401 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
2461 catggacgag ctgtacaagt aagaattcga tatcaagctt atcgataatc aacctctgga
2521 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
2581 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
2641 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
2701 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
2761 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
2821 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
2881 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
2941 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
3001 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
3061 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ccgggcggcc
3121 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
3181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
3241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
3301 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
3361 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca
3421 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
3481 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
3541 gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
3601 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
3661 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
3721 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
3781 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
3841 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
3901 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
3961 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
4021 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
4081 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
4141 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
4201 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
4261 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
4321 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
4381 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
4441 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
4501 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
4561 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
4621 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
4681 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
4741 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
4801 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
4861 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
4921 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
4981 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
5041 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
5101 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
5161 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
5221 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
5281 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
5341 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
5401 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
5461 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
5521 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
5581 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
5641 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
5701 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
5761 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
5821 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
5881 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
5941 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
6001 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
6061 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
6121 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
6181 actaggggtt ccttgtagtt aatgattaac ccgccatgct act
//
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