| 质粒名称: | pPGK-SB13 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 236078 |
| 存储实验室: | Masashi Narita |
|
载体骨架基本信息 |
|
| 载体名称: | pT |
| 载体出品公司: | Clontech |
| 空载体大小: | 4663bp |
| 完整质粒大小: | 4663bp |
| 载体类型: | Sleeping beauty (SB) transposase plasmid |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SB13 transposase |
| 物种来源: | Atlantic Salmon |
| 插入大小: | 1023 bp |
| 启动子: | PGK |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pPGK-SB13 was a gift from Masashi Narita (Addgene plasmid # 236078 ; http://n2t.net/addgene:236078 ; RRID:Addgene_236078) |
| 参考文献部分: | Titration of RAS alters senescent state and influences tumour initiation. Chan ASL, Zhu H, Narita M, Cassidy LD, Young ARJ, Bermejo-Rodriguez C, Janowska AT, Chen HC, Gough S, Oshimori N, Zender L, Aitken SJ, Hoare M, Narita M. Nature. 2024 Sep;633(8030):678-685. doi: 10.1038/s41586-024-07797-z. Epub 2024 Aug 7. 10.1038/s41586-024-07797-z PubMed 39112713 |
买家导航
pPGK-SB13载体质粒基本信息
pPGK-SB13质粒图谱载体图谱和pPGK-SB13载体序列质粒序列多克隆位点信息
pPGK-SB13质粒载体简介
pPGK-SB13质粒序列载体序列
LOCUS pPGK-SB13 4663 bp DNA circular SYN 17-APR-2025
DEFINITION SB13 transposase, for use with Sleeping Beauty transponson plasmids.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4663)
AUTHORS Chan ASL, Zhu H, Narita M, Cassidy LD, Young ARJ, Bermejo-Rodriguez
C, Janowska AT, Chen HC, Gough S, Oshimori N, Zender L, Aitken SJ,
Hoare M, Narita M
TITLE Titration of RAS alters senescent state and influences tumour
initiation.
JOURNAL Nature. 2024 Sep;633(8030):678-685. doi: 10.1038/s41586-024-07797-z.
Epub 2024 Aug 7.
PUBMED 39112713
REFERENCE 2 (bases 1 to 4663)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 17, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41586-024-07797-z"; journalName: "Nature"; date:
"2024-09"; volume: "633"; issue: "8030"; pages: "678-685"
COMMENT Sequence Label: pPGK-SB13
FEATURES Location/Qualifiers
source 1..4663
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 202..224
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 216..233
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 217..233
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 269..768
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind 701..720
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
intron 836..932
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 999..2021
/codon_start=1
/product="hyperactive variant of the Sleeping Beauty
transposase (Mates et al., 2009)"
/label=SB100X
/translation="MGKSKEISQDLRKKIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTAAKDLVKMLEETGTKVSISTVK
RVLYRHNLKGRSARKKPLLQNRHKKARLRFARAHGDKDRTFWRNVLWSDETKIELFGHN
DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMRKENYVDIL
KQHLKTSVRKLKLGRKWVFQMDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPTYCGKLVEGYPKRLTQVKQFKGNATKY
"
polyA_signal 2035..2169
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2059..2078)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2113..2132
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2280..2296)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2280..2296)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2293..2315)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2304..2320
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(2328..2334,2335..2352,2353..2358))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2373..2394
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2511..2528)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2682..3270)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2762..2781)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(3441..4232,4233..4301))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4064..4083
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4302..4406)
/gene="bla"
/label=AmpR promoter
primer_bind 4474..4492
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(4530..4552)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind join(4652..4663,1..8)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
61 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
121 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
181 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg
241 agctcggtac ccgggggcgc gccctaccgg gtaggggagg cgcttttccc aaggcagtct
301 ggagcatgcg ctttagcggc cccgctgggc acttggcgct acacaagtgg cctctggcct
361 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
421 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
481 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
541 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
601 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
661 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
721 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcatccat
781 ctagatcctt aattaagtct agagtcgagg aactgaaaaa ccagaaagtt aactggtaag
841 tttagtcttt ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac
901 tgctcctcag tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct
961 aaaagctgcg gaattgtacc cgcggccgat ccgacatcat gggaaaatca aaagaaatca
1021 gccaagacct cagaaaaaaa attgtagacc tccacaagtc tggttcatcc ttgggagcaa
1081 tttccaaacg cctggccgta ccacgttcat ctgtacaaac aatagtacgc aagtataaac
1141 accatgggac cacgcagccg tcataccgct caggaaggag acgcgttctg tctcctagag
1201 atgaacgtac tttggtgcga aaagtgcaaa tcaatcccag aacagccgca aaggaccttg
1261 tgaagatgct ggaggaaaca ggtacaaaag tatctatatc cacagtaaaa cgagtcctat
1321 atcgacataa cctgaaaggc cgctcagcaa ggaagaagcc actgctccaa aaccgacata
1381 agaaagccag actacggttt gcaagagcac atggggacaa agatcgtact ttttggagaa
1441 atgtcctctg gtctgatgaa acaaaaatag aactgtttgg ccataatgac catcgttatg
1501 tttggaggaa gaagggggag gcttgcaagc cgaagaacac catcccaacc gtgaagcacg
1561 ggggtggcag catcatgttg tgggggtgct ttgctgcagg agggactggt gcacttcaca
1621 aaatagatgg catcatgagg aaggaaaatt atgtggatat attgaagcaa catctcaaga
1681 catcagtcag gaagttaaag cttggtcgca aatgggtctt ccaaatggac aatgacccca
1741 agcatacttc caaagttgtg gcaaaatggc ttaaggacaa caaagtcaag gtattggagt
1801 ggccatcaca aagccctgac ctcaatccta tagaaaattt gtgggcagaa ctgaaaaagc
1861 gtgtgcgagc aaggaggcct acaaacctga ctcagttaca ccagctctgt caggaggaat
1921 gggccaaaat tcacccaact tattgtggga agcttgtgga aggctacccg aaacgtttga
1981 cccaagttaa acaatttaaa ggcaatgcta ccaaatacta gaattggccg cggggatcca
2041 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
2101 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
2161 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
2221 gaggtttttt cggatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg
2281 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
2341 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
2401 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
2461 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
2521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
2581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
2641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
2701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
2761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
2821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
2881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
2941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
3001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
3061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
3121 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
3181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
3241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
3301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
3361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
3421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
3481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
3541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
3601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
3661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
3721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
3781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
3841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
3901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
3961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
4021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
4081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
4141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
4201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
4261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
4321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
4381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
4441 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
4501 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
4561 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
4621 gttggcgggt gtcggggctg gcttaactat gcggcatcag agc
//
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