pCAG-mCherry-Tigre-target-array

LOCUS       pCAG-mCherry-Tigre-target-array               8203 bp DNA     circular SYN 04-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
     primer_bind     1769..1786
                     /label=mCherry Fwd Primer
     regulatory      1769..1778
                     /note="/label=Kozak sequence"
     CDS             1775..2485
                     /label=mCherry
     misc_feature    1855..1874
                     /label=qpcr mch fw
     misc_feature    1976..1999
                     /label=qpcr mch rev
                     
     misc_feature    2516..3091
                     /label=WPRE
     promoter        2565..2571
                     /label=lac promoter
     CDS             complement(2985..2996)
                     /label=Factor Xa site
     primer_bind     3068..3087
                     /label=RC1 fw
     primer_bind     3088..3103
                     /label=TC_NGS1_Bulk_R
     misc_feature    3114..3131
                     /label=mCherry forward
     misc_feature    3114..3131
                     /label=mch fw
     misc_feature    3137..3406
                     /label=Tigre CARLIN array
                     /label=mCherry array
     misc_feature    3137..3156
                     /label=TS9
     misc_feature    3164..3183
                     /label=TS2
     misc_feature    3191..3210
                     /label=TS4
     misc_feature    3218..3237
                     /label=TS8
     misc_feature    3245..3264
                     /label=TS3
     misc_feature    3272..3291
                     /label=TS5
     misc_feature    3299..3318
                     /label=TS7
     misc_feature    3326..3345
                     /label=TS1
     misc_feature    3353..3372
                     /label=TS10
     misc_feature    3380..3399
                     /label=TS6
     misc_feature    3408..3427
                     /label=mch rev
     misc_feature    3408..3427
                     /label=mCherry rev
     polyA_signal    3428..3549
                     /label=SV40 poly(A) signal
     primer_bind     complement(3538..3555)
                     /label=PolyA Rev primer
     protein_bind    3550..3583
                     /label=FRT (minimal)
     misc_feature    3585..3604
                     /label=2x HS4
     misc_feature    3629..4559
                     /label=TIGRE homology arm 1
     misc_feature    3629..3646
                     /label=gRNA
     primer_bind     complement(3821..3840)
                     /label=Genotype common
     primer_bind     complement(4567..4583)
                     /label=M13 fwd
     promoter        5057..5161
                     /label=AmpR promoter
     CDS             5162..5230
                     /label=AmpR
     CDS             5231..6022
                     /label=AmpR
     rep_origin      6193..6781
                     /label=ori
     protein_bind    7069..7090
                     /label=CAP binding site
     promoter        7105..7110
                     /label=lac promoter
     promoter        7111..7128
                     /label=lac promoter
     promoter        7129..7135
                     /label=lac promoter
     protein_bind    7143..7159
                     /label=lac operator
     primer_bind     7167..7183
                     /label=M13 rev
     misc_feature    7204..8187
                     /label=TIGRE homology 2
     primer_bind     8044..8063
                     /label=Genotype wt rev
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgagaattct
     1741 cacgcgtatt gggatattgc gatcgcgtgc caccatggtg agcaagggcg aggaggataa
     1801 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     1861 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     1921 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     1981 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     2041 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     2101 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     2161 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     2221 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     2281 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     2341 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     2401 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     2461 cggcatggac gagctgtaca agtaagaagg aattcgatat caagcttatc gataatcaac
     2521 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2581 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2641 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     2701 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     2761 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     2821 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     2881 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     2941 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3001 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3061 gccctcagac gagtcggatc tccctttggg cttcgagctc ggtacccggc cgcgctcggt
     3121 acctcgcgaa tcgccggagt cgagacgctg acgatatgga gtcgacacga ctcgcgcata
     3181 cgatggagtc gcgagcgcta tgagcgacta tggagtcgat agtatgcgta cacgcgatgg
     3241 agtcgactac agtcgctacg acgatggagt cgatacgata cgcgcacgct atggagtcgc
     3301 gactgtacgc acacgcgatg gagtcgactg cacgacagtc gacgatggag tcgatacgta
     3361 gcacgcagac gatgggagcg agagcgcgct cgtcgactat ggagtcttgt cggtgccttc
     3421 tagttgcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3481 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3541 tgtatcttag aagttcctat tctctagaaa gtataggaac ttcctaagct tgatatcgaa
     3601 ttcggcggcc gccctgcagg atttaaatgt taggtgttat ggcagtaact ttaattctag
     3661 cgatctggag gcagaggcag gtggatctat gagtttgagg ctggcttgct ctacacagtg
     3721 agttccagaa aagccagggc tacacagtga gaccctatct agaaacaaca acagcaacaa
     3781 aaaatataca catctctcct aaaaataaaa cacgcagtct ggaagtaagt gaccgttggg
     3841 gaagcactgg aaaggagtta gagaatgtag acctcttgtg cctgactggg tgtccggatt
     3901 caatagtgac ttctccggtg atctgggaat ctgtgaagag gttctggctc tctgtctagc
     3961 ctgaggctga taccccaact cattttcttg tcttctgtga ggaaggttac aactctagga
     4021 gtccccttcc tttggccagt gcttccctaa gaaagctatg gcttgattcc cacccccacc
     4081 ctacctccac tgccaagtct ctggacagat gtgcagcctc tggcagtggg agggaaggaa
     4141 ctggataaag tggtcctcat tcgtcagcct cactggcagg cccggaaagt gctgaactca
     4201 gggggctccg gagaggatga tgcagaggtg ggcctcttcc gaggccctga gtctagggtt
     4261 gccactgagt cttcctgagt cattttcttt gcccatggaa gaaaagaaaa gaaatttaaa
     4321 aaacaaaaac aaaacaaaaa accctaagtg gtttttggtg taaggatctg ggagttcctc
     4381 ctgttcgaag ctgttgggta ccttcctacc tcttcctacc acctcgccag atttcagatt
     4441 ccgaccttca gtggctcaaa tcccacttcc ctaacttcct agcagtctga cctcactttg
     4501 agtgataaca catatgagcc tcagtttcaa atgctagtat ccatctccta ggggcagtat
     4561 ttaaatactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4621 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     4681 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     4741 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     4801 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4861 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4921 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4981 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5041 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5101 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5161 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5221 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5281 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5341 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5401 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5461 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5521 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5581 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5641 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5701 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5761 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5821 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5881 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5941 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6001 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6061 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6121 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6181 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6241 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6301 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6361 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6421 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6481 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6541 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6601 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6661 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6721 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6781 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6841 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6901 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6961 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7021 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     7081 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7141 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccattta
     7201 aatgaaaagt ggagaggcgt gagtcggaga actaagaaga aacgcctcct ccagggctga
     7261 gctccgcccc taggctggcg tgctgcgtac tcccccagta ctcttggggg tgaggcgttt
     7321 gtcagaagga ggcgctcagc tactttatac acccggagga gggctggctc cgcccccagg
     7381 agggacggtt actagcagaa tgcagacggt gctctgcatc tttttgtcaa gggacggtgt
     7441 tctgatccac gaaaccctcc ctcggagagt tcgggcccct ccgggccagt gtcctgcaaa
     7501 caacctctcc tgagcttgag gaaagacaag aaccgtttcc aaacccaggt gattcggcca
     7561 cgcccccaag ttaaggttcc gccctgtacg ttcccaggta agctccgcct tgatcccgtc
     7621 ccactggtaa gaaaaggccc tgtgataggt gtgctctggt ttgcaggctc gcacccagct
     7681 ggggcccctt cccatgctgc tctgggataa aggcttttca agccgcgctc cagagcttgg
     7741 ccttcacaaa gaacttcaag gcagtaactt taattcttta gcttctccac taggacctct
     7801 ccttttcact agttaactct caggtccccc agtgaactca cagtggccag tcacttgttc
     7861 acttttgctg ggcctcttag aactctaatt tacgtacccc ctcctgggcc tccgcaagac
     7921 tcacaaaaga ctcaaccctt tcccaagtca tccctcccat ctaatggtgc aagcacacaa
     7981 tctcaggcag ccaggaccca caaagtgaag agtaaaaacg cactctgctg ggcagtggtg
     8041 gcacacacct ttaatcccga tgctcaggag gcagaggctg gcagtccttg agttccaggc
     8101 cagcctggtc tacagagtga gttctaggac agccaggact acactgaaaa accctatctc
     8161 aaaacaaaca gaaaacatcc caaaatttaa atggcgcgcc gtc
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