| 质粒名称: | pYK209 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000800 |
| 产品用途: | Mammalian PhCMV-driven constitutive NLS-FUS478-AG-NS3a(H1) expression vector (PhCMV-NLS-FUS478-AG-NS3a(H1)-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CMV
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NLS-FUS478-AG-NS3a(H1) |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK209
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK209载体质粒基本信息
pYK209质粒图谱载体图谱和pYK209载体序列质粒序列多克隆位点信息
pYK209质粒载体简介
pYK209质粒序列载体序列
LOCUS pYK209 8469 bp DNA circular SYN 18-MAY-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8469)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8469
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 213..232
/label=oYK403_MluI_F
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..984
/label=oCW60
primer_bind 881..989
/label=oYK209_FUS_F
primer_bind 881..928
/label=oYK449_NLS3_F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 881..900
/label=oYK30-NS3a_Head-F
promoter 881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 887..904
/label=oYK661_Nhe_F
primer_bind 902..921
/label=oYK809_NLS_F
misc_feature 905..964
/label=NLS
primer_bind 964..989
/label=oYK374_FUS_F
primer_bind 964..984
/label=oCW41
CDS 965..1609
/codon_start=1
/gene="FUS"
/gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2;
POMP75;TLS"
/product="RNA-binding protein FUS isoform 1"
/label=FUS
/note="isoform 1 is encoded by transcript variant 1;
heterogeneous nuclear ribonucleoprotein P2; fused in
sarcoma; fusion gene in myxoid liposarcoma; 75 kDa
DNA-pairing protein; RNA-binding protein FUS; oncogene TLS;
fus-like protein; oncogene FUS; translocated in liposarcoma
protein"
/db_xref="CCDS:CCDS10707.1"
/db_xref="GeneID:2521"
/db_xref="HGNC:HGNC:4010"
/db_xref="MIM:137070"
/protein_id="NP_004951.1"
/translation="MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQST
DTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQP
APSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNS
SSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGG"
primer_bind 965..991
/label=oYK397_FUS_F
primer_bind 965..989
/label=oYK525_FUS_F
primer_bind 968..993
/label=oYK339_FUS_F
misc_feature 1040..1042
/gene="FUS"
/gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2;
POMP75;TLS"
/note="Phosphoserine./evidence=ECO:0000269|PubMed:29897835;
propagated from UniProtKB/Swiss-Prot (P35637.1);
phosphorylation site"
misc_feature 1052..1054
/gene="FUS"
/gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2;
POMP75;TLS"
/note="Phosphoserine./evidence=ECO:0000269|PubMed:29897835;
propagated from UniProtKB/Swiss-Prot (P35637.1);
phosphorylation site"
misc_feature 1088..1090
/gene="FUS"
/gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2;
POMP75;TLS"
/note="Phosphoserine, by ATM.
/evidence=ECO:0000269|PubMed:18620545; propagated from
UniProtKB/Swiss-Prot (P35637.1); phosphorylation site"
misc_feature 1487..1489
/gene="FUS"
/gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2;
POMP75;TLS"
/note="Breakpoint for translocation to form
chimericFUS/ATF1 protein; propagated from
UniProtKB/Swiss-Prot (P35637.1); other site"
primer_bind complement(1579..1611)
/label=oCW42
primer_bind complement(1579..1611)
/label=oCW61
primer_bind 1590..1631
/label=oYK68_FUS2_F
primer_bind 1590..1609
/label=oYK69_AG_ANR1_F
primer_bind complement(1594..1609)
/label=oYK68_FUS1-R
misc_feature 1610..2398
/label=FUS215-478
primer_bind 1698..1712
/label=oYK68_FUS2_F
primer_bind complement(2377..2418)
/label=oYK88_FUS478_R
misc_feature 2399..2626
/label=Long_Linker
primer_bind 2399..2419
/label=oYK629_NS3a_F
primer_bind 2399..2418
/label=oYK78_AG_ANR1_F
primer_bind 2399..2416
/label=oYK69_AG_ANR1_F
primer_bind complement(2603..2623)
/label=oYK81_ANR_AG_R
misc_feature 2627..3301
/label=AG
primer_bind 3075..3085
/label=oSCL1104
primer_bind complement(3289..3309)
/label=oYK65-AG-R
misc_feature 3302..3352
/label=Short_Linker
primer_bind complement(3328..3352)
/label=oYK33-35-PB1-AG-R
primer_bind 3333..3373
/label=oYK153_NS3a_F
CDS 3353..3940
/codon_start=1
/label=NS3a H1
/translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
primer_bind 3353..3388
/label=oSCL1082
primer_bind 3353..3373
/label=oyk03-03-F
primer_bind 3353..3373
/label=oYK04-ns3a-F
primer_bind 3353..3371
/label=oYK25-NS3a-F
primer_bind 3353..3371
/label=oYK629_NS3a_F
primer_bind 3353..3370
/label=oYK30-NS3a_Head-F
primer_bind 3370..3428
/label=oSCL1083
primer_bind 3409..3467
/label=oSCL1084
primer_bind 3446..3504
/label=oSCL1085
primer_bind 3483..3541
/label=oSCL1086
primer_bind complement(3511..3530)
/label=oSCL1106
primer_bind 3512..3550
/label=oSCL1104
primer_bind complement(3747..3805)
/label=oSCL1105
primer_bind complement(3787..3858)
/label=oSCL1087
primer_bind 3789..3810
/label=oSCL1107
primer_bind 3840..3858
/label=oSCL1088
primer_bind complement(3922..3969)
/label=oSCL1089
primer_bind complement(3925..3972)
/label=oYK30-NS3a_head-R
primer_bind complement(3928..3946)
/label=oYK319_NS3a_R
primer_bind complement(3928..3946)
/label=oYK374_NS3a_R
primer_bind complement(3928..3946)
/label=oYK405_NS3a_R
/note="for 3XFLAG"
primer_bind complement(3928..3946)
/label=oYK809_NS3a_R
primer_bind complement(3941..3989)
/label=oYK03-03-R
primer_bind complement(3941..3960)
/label=oSCL1130
primer_bind complement(3949..3969)
/label=olyl382
primer_bind complement(3970..3992)
/label=oYK57-MCP-mCherry-R
primer_bind complement(3970..3989)
/label=oYK25-NS3a-R
primer_bind complement(3994..4013)
/label=oSCL1089
terminator 4066..4293
/label=bGH PA
misc_feature 4339..4767
/label=f1 origin
misc_feature 4772..5115
/label=SV40 early promoter and origin
misc_feature 4961..5038
/label=SV40 ORI
CDS 5180..5971
/label=Kan/neoR
misc_feature 6145..6275
/label=SV40 early polyadenylation signal
primer_bind 6249..6268
/label=olyl382
primer_bind complement(6311..6331)
/label=M13-rev
misc_binding complement(6337..6359)
/label=LacO
promoter complement(6364..6393)
/label=lac
primer_bind 6508..6519
/label=oYK69_AG_ANR1_F
misc_feature complement(6658..7328)
/label=pUC origin
CDS complement(7476..8135)
/label=AmpR
primer_bind 7792..7803
/label=oyk03-03-F
primer_bind 7792..7803
/label=oYK04-ns3a-F
primer_bind 7792..7803
/label=oYK153_NS3a_F
promoter complement(8375..8403)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
961 agtcatggcc tcaaacgatt atacccaaca agcaacccaa agctatgggg cctaccccac
1021 ccagcccggg cagggctatt cccagcagag cagtcagccc tacggacagc agagttacag
1081 tggttatagc cagtccacgg acacttcagg ctatggccag agcagctatt cttcttatgg
1141 ccagagccag aacacaggct atggaactca gtcaactccc cagggatatg gctcgactgg
1201 cggctatggc agtagccaga gctcccaatc gtcttacggg cagcagtcct cctaccctgg
1261 ctatggccag cagccagctc ccagcagcac ctcgggaagt tacggtagca gttctcagag
1321 cagcagctat gggcagcccc agagtgggag ctacagccag cagcctagct atggtggaca
1381 gcagcaaagc tatggacagc agcaaagcta taatccccct cagggctatg gacagcagaa
1441 ccagtacaac agcagcagtg gtggtggagg tggaggtgga ggtggaggta actatggcca
1501 agatcaatcc tccatgagta gtggtggtgg cagtggtggc ggttatggca atcaagacca
1561 gagtggtgga ggtggcagcg gtggctatgg acagcaggac cgtggaggca gaggcagagg
1621 aggaagcgga ggaggaggag ggggaggagg aggcggatac aacagaagca gcggaggata
1681 cgagccaaga ggaagaggag gaggaagagg agggagagga ggaatgggag gaagcgacag
1741 aggaggattc aacaagttcg gaggaccaag agaccaggga agcagacacg acagcgagca
1801 ggacaacagc gacaacaaca caatcttcgt gcagggactg ggcgagaatg tgaccatcga
1861 gagcgtggcc gactacttca agcagatcgg aatcatcaag acaaacaaga aaacaggaca
1921 gcccatgatc aacctgtaca ccgacagaga gactggcaag ctgaagggag aggccaccgt
1981 gagcttcgac gaccccccca gcgccaaagc cgccatcgac tggttcgacg gcaaggagtt
2041 cagcggcaac ccaatcaaag tgagctttgc caccagaaga gccgacttca acagaggcgg
2101 aggcaacgga agaggcggaa gaggaagagg ggggccaatg ggcagaggag ggtatggcgg
2161 aggaggaagc gggggaggag ggagaggggg attcccaagc ggaggagggg ggggaggagg
2221 ccagcagaga gctggagact ggaagtgccc aaacccaaca tgcgagaaca tgaacttcag
2281 ctggagaaac gagtgcaacc agtgcaaggc cccaaagcca gatggacccg gcggcggacc
2341 aggaggaagc catatgggcg gcaactacgg cgacgacaga aggggcggca gaggcggagg
2401 ttctgggagt ggcggtagtg gtgctggagg cagcgcaggc tctggagcag gcggcagtgc
2461 cggaagtggc gctggagggt ctgccggatc tggagccggt ggttctgccg gctccggcgc
2521 tggtgggagc gcttccggaa gtagtcccgg taattccgct gacggcggcg gaggatcggg
2581 tggtagtggt ggttcaggag gaggatcgac ccaaggagga tccatggtga gcgtgatcaa
2641 gcccgagatg aagatcaagc tgtgcatgag gggcaccgtg aacggccaca acttcgtgat
2701 cgagggcgag ggcaagggca acccctacga gggcacccag atcctggacc tgaacgtgac
2761 cgagggcgcc cccctgccct tcgcctacga catcctgacc accgtgttcc agtacggcaa
2821 cagggccttc accaagtacc ccgccgacat ccaggactac ttcaagcaga ccttccccga
2881 gggctaccac tgggagagga gcatgaccta cgaggaccag ggcatctgca ccgccaccag
2941 caacatcagc atgaggggcg actgcttctt ctacgacatc aggttcgacg gcgtgaactt
3001 cccccccaac ggccccgtga tgcagaagaa gaccctgaag tgggagccca gcaccgagaa
3061 gatgtacgtg agggacggcg tgctgaaggg cgacgtgaac atggccctgc tgctggaggg
3121 cggcggccac tacaggtgcg acttcaagac cacctacaag gccaagaagg acgtgaggct
3181 gcccgactac cacttcgtgg accacaggat cgagatcctg aagcacgaca aggactacaa
3241 caaggtgaag ctgtacgaga acgccgtggc caggtacagc atgctgccca gccaggccaa
3301 gggtaccgga actgcagcag agaattcggg aaactcgaga acaaagcttg aaaagaagaa
3361 gggcagcgtg gtgatcgtgg gcagaatcaa cctgagcggc gacaccgcct acagccagca
3421 gaccagaggc ctggagggct gccaggagac cagccagacc ggcagagaca agaaccaggt
3481 ggagggcgag gtgcaggtgg tgagcaccgc cacccagagc ttcctggcca ccagcatcaa
3541 cggcgtgctg tggaccgtgt accacggcgc cggcaccaga accatcgcca gccccaaggg
3601 ccccgtgacc cagatgtaca cgaacgtgga caaggactta gtgggctggc aggcccccca
3661 aggcagcaga agcctgaccc cctgcacctg cggcagcagc gacctgtacc tggtgaccag
3721 acacgccgac gtgatccccg tgagaggaag aggcgacagc agaggcagcc tgctgagccc
3781 cagacccatc agctatctga aaggctcaag cggcggcccc ctgttgtgtc ccgccggcca
3841 tgccgtgggc atcttcagag ccgccgtgag caccagaggc gtggccaagg ccgtggactt
3901 catccccgtg gagagcctgg agaccaccat gagaagcccc gctagctaga aattctgcag
3961 atatccagcg gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
4021 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
4081 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
4141 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
4201 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
4261 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
4321 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
4381 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
4441 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
4501 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
4561 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
4621 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
4681 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
4741 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
4801 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
4861 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
4921 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
4981 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
5041 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
5101 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
5161 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
5221 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
5281 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
5341 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
5401 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
5461 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
5521 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
5581 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
5641 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
5701 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
5761 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
5821 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
5881 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
5941 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
6001 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
6061 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
6121 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
6181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
6241 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
6301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
6361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
6421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
6481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
6541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
6601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
6661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
6721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
6781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
6841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
6901 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
6961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
7021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
7081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
7141 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
7201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt
7261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
7321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
7381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
7441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
7501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
7561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
7621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
7681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
7741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
7801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
7861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
7921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
7981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
8041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
8101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
8161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
8221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
8281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
8341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
8401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
8461 cctgacgtc
//
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