pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA3-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA3-FLAG               7005 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3718
                     /codon_start=1
                     /gene="PLXNA3"
                     /gene_synonym="6.3; HSSEXGENE; PLXN3; PLXN4; XAP-6"
                     /product="plexin-A3 precursor"
                     /label=PLXNA3
                     /note="Sex chromosome X transmembrane protein of 
                     HGFreceptor family 3; plexin-4; semaphorin receptor SEX"
                     /db_xref="GI:533112479"
                     /db_xref="CCDS:CCDS14752.1"
                     /db_xref="GeneID:55558"
                     /db_xref="HGNC:HGNC:9101"
                     /db_xref="MIM:300022"
                     /protein_id="NP_059984.3"
                     /translation="GKNLIPAAAGSSRLNYTVLIGGQPCSLTVSDTQLLCDSPSQTGRQ
                     PVMVLVGGLEFWLGTLHISAERALTLPAMMGLAAGGGLLLLAITAVLVAYKRKTQDADR
                     TLKRLQLQMDNLESRVALECKEAFAELQTDINELTNHMDEVQIPFLDYRTYAVRVLFPG
                     IEAHPVLKELDTPPNVEKALRLFGQLLHSRAFVLTFIHTLEAQSSFSMRDRGTVASLTM
                     VALQSRLDYATGLLKQLLADLIEKNLESKNHPKLLLRRTESVAEKMLTNWFTFLLHKFL
                     KECAGEPLFLLYCAIKQQMEKGPIDAITGEARYSLSEDKLIRQQIDYKTLTLHCVCPEN
                     EGSAQVPVKVLNCDSITQAKDKLLDTVYKGIPYSQRPKAEDMDLEWRQGRMTRIILQDE
                     DVTTKIECDWKRLNSLAHYQVTDGSLVALVPKQVSAYNMANSFTFTRSLSRYESLLRTA
                     SSPDSLRSRAPMITPDQETGTKLWHLVKNHDHADHREGDRGSKMVSEIYLTRLLATKGT
                     LQKFVDDLFETVFSTAHRGSALPLAIKYMFDFLDEQADQRQISDPDVRHTWKSNCLPLR
                     FWVNVIKNPQFVFDIHKNSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIP
                     NYKSWVERYYRDIAKMASISDQDMDAYLVEQSRLHASDFSVLSALNELYFYVTKYRQEI
                     LTALDRDASCRKHKLRQKLEQIISLVSSDS"
     misc_feature    1606..1611
                     /label=SDS
     regulatory      2208..2217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3725..3790
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3794..4225
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3938..3943
                     /label=SDS
     repeat_region   4268..4408
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4268..4397
                     /label=AAV2 ITR
     repeat_region   4269..4409
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4483..4938
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5220..5324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5325..6185
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6356..6944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca agaacctgat
     1561 tcccgcggca gccggcagct cccgcctcaa ctacactgtg ctgataggag gccagccgtg
     1621 ttcgctcact gtctcggaca cacaactcct gtgcgactca cccagccaga ctggccggca
     1681 gcctgtcatg gtgctggtgg gtggcctgga gttctggctg ggcaccctgc acatctcggc
     1741 agagcgggcg ctgaccctac cggccatgat ggggctggcg gcggggggtg ggctcctgct
     1801 gctggccatc acagccgtgc tggtggcgta caagcgcaag actcaggacg cggaccgtac
     1861 cctcaagcgt ctgcagctgc agatggacaa cctggagtcc cgtgtggccc tggagtgcaa
     1921 ggaagctttt gcagagctgc agacggacat caatgagctg actaaccaca tggacgaggt
     1981 gcagatcccc ttcctggact accggactta cgccgtgcgc gtgctcttcc cgggcatcga
     2041 ggcccacccg gtgctcaagg agctggatac gccacccaac gtggagaagg ccctgcgcct
     2101 cttcgggcag ctgctgcaca gccgcgcgtt cgtgcttacc ttcatccaca cgctggaggc
     2161 ccagagcagc ttctccatgc gcgaccgcgg caccgtggcc tcgctcacca tggtggccct
     2221 gcagagccgg ctcgactatg ccacggggct gctcaagcaa ctgctggccg acctcatcga
     2281 gaagaacctc gagagcaaga accaccccaa gctgctgcta cgcaggacag agtcagtggc
     2341 tgagaagatg cttaccaact ggttcacgtt cctgctgcat aagtttctga aggagtgtgc
     2401 tggggagcct ctcttcctgc tttactgtgc catcaagcag cagatggaga agggccccat
     2461 tgatgccatc acgggcgagg cacgatactc cctgagcgag gacaagctca tccgtcagca
     2521 gatcgactac aagacactga cccttcactg cgtgtgtccg gagaacgagg gcagcgccca
     2581 ggtcccagtg aaggttctca actgtgacag catcacccag gccaaagata agctgctgga
     2641 cactgtgtac aagggcattc cgtactccca gcgtcccaaa gctgaggaca tggacctgga
     2701 gtggcgccag ggccgcatga ctcgcatcat cctccaggat gaggatgtca ccaccaagat
     2761 cgagtgtgac tggaagaggc tcaactcact ggcccactac caggtgacag acggttcctt
     2821 ggtggcattg gtgcccaaac aagtgtctgc ctataacatg gccaactcct tcaccttcac
     2881 ccgctccctc agccgctacg agagcttgct ccgcacggcc agcagccctg atagcctccg
     2941 ctcacgggca cccatgatta cgcctgacca ggagacaggc accaaattgt ggcacctggt
     3001 gaaaaaccac gaccatgccg accatcgcga gggggaccgt ggcagcaaga tggtctccga
     3061 gatctacctg acacggctgc tggccaccaa gggcacactg cagaagttcg tggatgacct
     3121 ctttgagaca gtgttcagca cagcccaccg gggctcggcc ctgcccctgg ccatcaagta
     3181 catgttcgac ttcctggatg agcaggcgga ccagcgccag atcagcgacc ccgatgtgcg
     3241 ccacacctgg aagagcaact gcctgccgct gcgcttctgg gtgaatgtga tcaagaaccc
     3301 gcagttcgtg ttcgacatcc acaagaacag catcacggat gcctgcctgt cggtggtagc
     3361 ccagaccttc atggactcct gctctacatc cgagcaccgc ctggggaagg actcgccctc
     3421 caacaaactg ctctacgcca aggacatccc caactacaag agctgggtgg agaggtatta
     3481 tcgagacatt gcaaagatgg catccatcag cgaccaggac atggatgcct acctggtgga
     3541 gcagtcccgc ctccacgcca gcgacttcag cgtcctgagt gcgctcaacg agctgtattt
     3601 ctatgtcacc aagtaccgcc aggagattct cacggctctg gaccgagatg cctcttgtcg
     3661 gaagcataag ttgcggcaga aactggaaca gatcatcagc ctcgtgtcca gcgacagctg
     3721 tacagactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     3781 cgatgacaag taactcgaga taatcaacct ctggattaca aaatttgtga aagattgact
     3841 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3901 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta
     3961 gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4021 ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta
     4081 tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4141 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4201 gggggagatg tgggaggttt tttaaagatc tggagaccgg tgtggcacgt gcggaccgag
     4261 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     4321 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4381 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4441 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     4501 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     4561 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4621 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4681 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4741 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4801 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4861 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4921 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4981 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5041 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5101 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5161 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5221 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5281 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5341 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5401 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5461 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5521 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5581 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5641 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5701 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5761 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5821 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5881 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5941 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6001 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6061 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6121 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6181 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6241 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6301 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6361 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6421 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6481 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6541 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6601 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6661 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6721 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6781 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6841 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6901 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6961 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//