| 质粒名称: | pLM-CMV-R-Cre |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 27546 |
| 存储实验室: | Michel Sadelain |
|
载体骨架基本信息 |
|
| 载体名称: | pLM (Sadelain lab) |
| 载体出品公司: | -- |
| 空载体大小: | 6711 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体,Cre/Lox |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mCherry_P2A_Cre recombinase |
| 物种来源: | -- |
| 插入大小: | 1806 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AgeI（在克隆过程中销毁） |
| 3’克隆位点: | SalI (未破坏) |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLM-CMV-R-Cre was a gift from Michel Sadelain (Addgene plasmid # 27546 ; http://n2t.net/addgene:27546 ; RRID:Addgene_27546) |
| 参考文献部分: | Genomic safe harbors permit high I. Papapetrou EP, Lee G, Malani N, Setty M, Riviere I, Tirunagari LM, Kadota K, Roth SL, Giardina P, Viale A, Leslie C, Bushman FD, Studer L, Sadelain M. Nat Biotechnol. 2010 Dec 12. ():. 10.1038/nbt.1717 PubMed 21151124 |
买家导航
pLM-CMV-R-Cre载体质粒基本信息
pLM-CMV-R-Cre质粒图谱载体图谱和pLM-CMV-R-Cre载体序列质粒序列多克隆位点信息
pLM-CMV-R-Cre质粒载体简介
pLM-CMV-R-Cre质粒序列载体序列
LOCUS pLM-CMV-R-Cre 8522 bp ds-DNA circular SYN 10-JUN-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLM-CMV-R-Cre
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8522)
AUTHORS Papapetrou EP, Lee G, Malani N, Setty M, Riviere I, Tirunagari LM,
Kadota K, Roth SL, Giardina P, Viale A, Leslie C, Bushman FD, Studer
L, Sadelain M
TITLE Genomic safe harbors permit high I
JOURNAL Nat Biotechnol. 2010 Dec 12. ():.
PUBMED 21151124
REFERENCE 2 (bases 1 to 8522)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 10, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8522
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 40..57
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 174..195
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 210..240
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 248..264
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 253..275
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 272..288
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 272..288
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 307..327
/label=T3
/note="T3 promoter, forward primer"
promoter 309..327
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 355..581
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 582..762
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 809..934
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1427..1660
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1845..1889
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2156..2273
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2350..2653
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2654..2857
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2808..2828
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 2895..3602
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(3040..3058)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 3308..3327
/label=mCherry-F
/note="mCherry, forward primer"
CDS 3612..3668
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 3669..4700
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
misc_feature 4707..5295
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4760..4780)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5178..5189)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5448..5628
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 5700..5821
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5737..5756)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5791..5810
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5861..5996
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5923..5942
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6016..6035)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6017..6035)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6045..6062)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6045..6061)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6054..6076)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6203..6658
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6290..6309)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6500..6521
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6684..6788
/gene="bla"
/label=AmpR promoter
CDS 6789..7649
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7007..7026)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7820..8408
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8309..8328
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
61 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
121 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
181 agttagctca ctcattaggc accccaggtt ttacacttta tgcttccggc tcgtatgttg
241 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
301 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
361 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
421 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
481 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
541 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
601 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
721 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
781 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
841 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
901 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
961 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
1021 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
1081 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
1141 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
1201 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
1261 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
1321 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
1381 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
1441 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
1501 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
1561 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
1621 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
1681 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
1741 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
1801 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
1861 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
1921 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
1981 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
2041 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
2101 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta
2161 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
2221 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc
2281 acgagactag cctcgataat agtaatcaat tacggggtca ttagttcata gcccatatat
2341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
2401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
2461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
2521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
2581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
2641 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
2701 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
2761 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
2821 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
2881 ctagcgctac cggtatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
2941 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
3001 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
3061 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
3121 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
3181 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
3241 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
3301 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
3361 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
3421 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
3481 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
3541 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
3601 agggatctgg agcaacaaac ttctcactac tcaaacaagc aggtgacgtg gaggagaatc
3661 ccggccctat gtccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg
3721 caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt
3781 ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt
3841 tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc
3901 ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc
3961 ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc
4021 ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg
4081 aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc tgccaggata
4141 tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg
4201 ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg
4261 gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa
4321 ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc
4381 tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa
4441 ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg
4501 actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag
4561 atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg
4621 taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc
4681 tgctggaaga tggcgattag gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
4741 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
4801 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
4861 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
4921 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
4981 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
5041 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
5101 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
5161 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
5221 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
5281 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc
5341 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
5401 ccaacgaaga caagatatcc tgctagctcc ggatccggac ctgtactggg tctctctggt
5461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
5521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
5581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
5641 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
5701 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
5761 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
5821 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
5881 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
5941 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
6001 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
6061 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
6121 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
6181 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
6241 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
6301 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
6361 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
6421 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
6481 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
6541 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
6601 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
6661 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
6721 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
6781 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
6841 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
6901 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
6961 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
7021 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
7081 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
7141 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
7201 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
7261 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
7321 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
7381 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
7441 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
7501 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
7561 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
7621 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
7681 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
7741 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
7801 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
7861 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
7921 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
7981 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
8041 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
8101 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
8161 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
8221 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
8281 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
8341 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
8401 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
8461 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
8521 ag
//
