pHGZ-ZKG

LOCUS       pHGZ-ZKG               6814 bp DNA     circular SYN 22-JUL-2026
DEFINITION  2762_TSM20260703-0571-00691_20260704-0571-BAN17-1_D09.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        21..96
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      100..119
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     CDS             505..1365
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1536..2124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2462..2481
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     misc_RNA        complement(2485..2560)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2906..3211
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADPSHRENDIKNAINLMFWGI"
     promoter        complement(3569..3837)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     promoter        3844..4187
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        4188..4235
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     terminator      4653..4850
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      5353..6230
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6546..6814
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
ORIGIN
        1 gccgcgatta aattccaaca gttttagagc tagaaatagc aagttaaaat aaggctagtc
       61 cgttatcaac ttgaaaaagt ggcaccgagt cggtggtgct ttttttgttt tttatgtctc
      121 gtcacatcgg ataataatga tggcagccat tgtagaagtg ccttttgcat ttctagtctc
      181 tttctcggtc tagctagttt tactacatcg cgaagataga atcttagatc acactgcctt
      241 tgctgagctg gatcaataga gtaacaaaag agtggtaagg cctcgttaaa ggacaaggac
      301 ctgagcggaa gtgtatcgta cagtagacgg agtatactag gtatagtcta tagtccgtgg
      361 aattaattct catgtttgac agcttatcat cgataagcta gcttttcaaa aaactgtatt
      421 ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt
      481 attacccatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1021 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1201 tctggagccg gtgagcgtgg gtcacgcggt atcattgcag cactggggcc agatggtaag
     1261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1681 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2281 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2341 tggccgattc attaatgcag ggcctgcggc cctctagatg catgctcgag cggccgccag
     2401 tgtgatggat atctgcagaa ttcgtcgacg agctcggtac caagcttgga tcctaggatc
     2461 tagacataaa aaacaaaaaa agcaccaccg actcggtgcc actttttcaa gttgataacg
     2521 gactagcctt attttaactt gctatttcta gctctaaaac cgagacccag ggatccggct
     2581 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     2641 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
     2701 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     2761 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     2821 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     2881 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     2941 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     3001 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     3061 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     3121 ggtctccgtt atcggggaag aagtggctga tcccagccac cgcgaaaatg acatcaaaaa
     3181 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     3241 tgcaggtcga tacagtagaa attacagaaa ctttatcacg tttagtaagt atagaggctg
     3301 aaaatccaga tgaagccgaa cgacttgtaa gagaaaagta taagagttgt gaaattgttc
     3361 ttgatgcaga tgattttcag gactatgaca ctagcgtata tgaataggta gatgttttta
     3421 ttttgtcaca caaaaaagag gctcgcacct ctttttctta tttcttttta tgatttaata
     3481 cggcattgag gacaatagcg agtaggctgg atacgacgat tccgtttgag aagaacattt
     3541 ggaaggctgt cggtcgacct gggtctcaga tcatttatct ttcactgcgg agaagtttcg
     3601 aacgccgaaa catgcgcacc aactttcact tctacagcgt ttgaccaaaa tcttttgaac
     3661 agaacattgt agggtgtgaa aaaatgcgca cctttaccgc tagcccaaga gggcactaca
     3721 aaatctagag ttgtacttca aacgtacatg taatcacctt gtatatactc gaaagaaaac
     3781 atcaagtttc tgtataaata tgagtgaaag cataatcata cattatcttt tcaaagaact
     3841 agtgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     3901 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     3961 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcggacctgc
     4021 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
     4081 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
     4141 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccgtt gacaattaat
     4201 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     4261 attgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     4321 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     4381 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     4441 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     4501 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     4561 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     4621 ggactgagaa ttcccgggga tcctctatca gtactgacaa taaaaagatt cttgttttca
     4681 agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta
     4741 gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc
     4801 agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ggatctctca
     4861 cttaatcttc tgtactctga agaggagtgg gaaataccaa gaaaaacatc aaactcgaat
     4921 gattttccca aacccctacc acaagatatt catcagggct ttccccgtca agctctaaat
     4981 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5041 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5101 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5161 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5221 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5281 atttaaatat ttgcttatac aatcttcctg tttttggggc ttttctgatt atcaaccggg
     5341 gtggagcttc ccattgcgaa taccgcttcc acaaacattg ctcaaaagta tctctttgct
     5401 atatatctct gtgctatatc cctatataac ctacccatcc acctttcgct ccttgaactt
     5461 gcatctaaac tcgacctcta cattttttat gtttatctct agtattactc tttagacaaa
     5521 aaaattgtag taagaactat tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt
     5581 tcctatacgt agtatataga gacaaaatag aagaaaccgt tcataatttt ctgaccaatg
     5641 aagaatcatc aacgctatca ctttctgttc acaaagtatg cgcaatccac atcggtatag
     5701 aatataatcg gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag
     5761 taaacgcggg aagtggagtc aggctttttt tatggaagag aaaatagaca ccaaagtagc
     5821 cttcttctaa ccttaacgga cctacagtgc aaaaagttat caagagactg cattatagag
     5881 cgcacaaagg agaaaaaaag taatctaaga tgctttgtta gaaaaatagc gctctcggga
     5941 tgcatttttg tagaacaaaa aagaagtata gattctttgt tggtaaaata gcgctctcgc
     6001 gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct
     6061 cgcgttgcat ttttgtttta caaaaatgaa gcacagattc ttcgttggta aaatagcgct
     6121 ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
     6181 gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt aacaaagata
     6241 tgctattgaa gtgcaagatg gaaacgcaga aaatgaaccg gggatgcgac gtgcaagatt
     6301 acctatgcaa tagatgcaat agtttctcca ggaaccgaaa tacatacatt gtcttccgta
     6361 aagcgctaga ctatatatta ttatacaggt tcaaatatac tatctgtttc agggaaaact
     6421 cccaggttcg gatgttcaaa attcaatgat gggtaacaag tacgatcgta aatctgtaaa
     6481 acagtttgtc ggatattagg ctgtatctcc tcaaagcgta ttcgaatatc attgagaagc
     6541 tgcagtcttt gaaaagataa tgtatgatta tgctttcact catatttata cagaaacttg
     6601 atgttttctt tcgagtatat acaaggtgat tacatgtacg tttgaagtac aactctagat
     6661 tttgtagtgc cctcttgggc tagcggtaaa ggtgcgcatt ttttcacacc ctacaatgtt
     6721 ctgttcaaaa gattttggtc aaacgctgta gaagtgaaag ttggtgcgca tgtttcggcg
     6781 ttcgaaactt ctccgcagtg aaagataaat gatc
//