| 质粒名称: | MSCV-hCD19 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 127889 |
| 存储实验室: | Anjana Rao |
|
载体骨架基本信息 |
|
| 载体名称: | MSCV |
| 载体出品公司: | Clontech |
| 空载体大小: | 5097bp |
| 完整质粒大小: | 6102 bp |
| 载体类型: | Retroviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | hCD19 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1005bp |
| 基因突变: | 无 |
| 标签/融合蛋白: | no tags |
|
文献引用方法 |
|
| 材料和方法部分: | MSCV-hCD19 was a gift from Anjana Rao (Addgene plasmid # 127889 ; http://n2t.net/addgene:127889 ; RRID:Addgene_127889) |
| 参考文献部分: | NR4A transcription factors limit CAR T cell function in solid tumours. Chen J, Lopez-Moyado IF, Seo H, Lio CJ, Hempleman LJ, Sekiya T, Yoshimura A, Scott-Browne JP, Rao A. Nature. 2019 Mar;567(7749):530-534. doi: 10.1038/s41586-019-0985-x. Epub 2019 Feb 27. 10.1038/s41586-019-0985-x PubMed 30814732 |
买家导航
MSCV-hCD19载体质粒基本信息
MSCV-hCD19质粒图谱载体图谱和MSCV-hCD19载体序列质粒序列多克隆位点信息
MSCV-hCD19质粒载体简介
MSCV-hCD19质粒序列载体序列
LOCUS MSCV-hCD19 6071 bp DNA circular SYN 14-APR-2025
DEFINITION Expresses hCD19.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6071)
AUTHORS Chen J, Lopez-Moyado IF, Seo H, Lio CJ, Hempleman LJ, Sekiya T,
Yoshimura A, Scott-Browne JP, Rao A
TITLE NR4A transcription factors limit CAR T cell function in solid
tumours.
JOURNAL Nature. 2019 Mar;567(7749):530-534. doi: 10.1038/s41586-019-0985-x.
Epub 2019 Feb 27.
PUBMED 30814732
REFERENCE 2 (bases 1 to 6071)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41586-019-0985-x"; journalName: "Nature"; date: "2019-03";
volume: "567"; issue: "7749"; pages: "530-534"
COMMENT Sequence Label: MSCV-hCD19
FEATURES Location/Qualifiers
source 1..6071
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 579..920
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 987..1403
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1333..1355
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1371..1387
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
RBS 1441..1452
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
LTR 2464..2978
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 3140..3156
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(3164..3170,3171..3188,3189..3194))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3209..3230
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3347..3364)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3518..4106)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3598..3617)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(4277..5068,5069..5137))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4900..4919
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5138..5242)
/gene="bla"
/label=AmpR promoter
primer_bind 5310..5328
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5366..5388)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5488..5507
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5701..5723
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5851..5870
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR join(6070..6071,1..515)
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
1381 ccctcactcc ttctctaggc gccggaatta gccaccatgc ctcccccacg actgttgttt
1441 tttctcctct ttctgacacc aatggaggtg cgcccagaag agcctctggt ggttaaggta
1501 gaagaagggg ataatgccgt actccaatgc cttaagggga catcagatgg accaactcaa
1561 cagttgacct ggagcaggga gtcccctctc aaaccttttc ttaagttgtc acttggcctt
1621 ccagggctcg gtatccatat gagacccctc gcaatctggt tgttcatctt taacgtctct
1681 cagcagatgg gagggtttta cctttgccag ccaggaccac ctagcgaaaa ggcttggcaa
1741 ccaggttgga ctgtcaatgt tgagggaagc ggtgagttgt ttcgctggaa cgtaagcgac
1801 ctgggggggc tcggatgtgg attgaaaaat cgatcctccg aagggccttc ttccccttca
1861 ggcaagttga tgtcccccaa actgtatgta tgggccaagg atcggcccga gatttgggaa
1921 ggagaacccc catgcttgcc tccaagagac agtcttaatc agtccctttc acaggacttg
1981 actatggccc caggtagtac cttgtggctg tcatgcggtg tgccccctga tagtgtgtct
2041 cggggtcctc tgagctggac ccacgtccat cccaaaggac caaaatccct tctttcactc
2101 gaattgaaag acgaccgccc tgcacgcgac atgtgggtga tggagactgg ccttttgctc
2161 ccccgagcca cagctcagga tgcaggaaag tactactgtc atcgaggtaa cctgactatg
2221 tcctttcacc tggaaatcac tgcacgccca gtgctttggc attggttgct cagaacagga
2281 ggctggaagg ttagcgcagt cacattggct tatctgatct tctgtctttg tagccttgtc
2341 ggaatactgc acctgcagcg agcccttgtg ctcagacgca agcggaagag aatgactgat
2401 ccaacaagaa ggttctgata aataaaataa aagattttat ttagtctcca gaaaaagggg
2461 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
2521 gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga acagagagac
2581 agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
2641 aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat
2701 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
2761 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
2821 acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtgtatcc
2881 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
2941 tctgagtgat tgactacccg tcagcggggg tctttcatgg gtaacagttt cttgaagttg
3001 gagaacaaca ttctgagggt aggagtcgaa tattaagtaa tcctgactca attagccact
3061 gttttgaatc cacatactcc aatactcctg aaatagttca ttatggacag cgcagaaaga
3121 gctggggaga attgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
3181 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
3241 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
3301 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
3361 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
3421 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
3481 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
3541 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
3601 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
3661 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
3721 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
3781 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
3841 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
3901 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
3961 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
4021 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
4081 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
4141 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
4201 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
4261 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
4321 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
4381 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
4441 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
4501 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
4561 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
4621 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
4681 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
4741 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
4801 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
4861 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
4921 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
4981 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
5041 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
5101 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
5161 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
5221 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
5281 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
5341 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
5401 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
5461 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
5521 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
5581 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
5641 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
5701 cccagtcacg acgttgtaaa acgacggcgc aaggaatggt gcatgcaagg agatggcgcc
5761 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
5821 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
5881 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagaggc gattagtcca
5941 atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca
6001 gctgaagcct atagagtacg agccatagat aaaataaaag attttattta gtctccagaa
6061 aaagggggga a
//
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